期刊文献+
共找到2,512篇文章
< 1 2 126 >
每页显示 20 50 100
Dietary fat supplementation relieves cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis in pigs
1
作者 Wei He Xinyu Liu +4 位作者 Ye Feng Hongwei Ding Haoyang Sun Zhongyu Li Baoming Shi 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第4期1669-1687,共19页
Background Cold stress has negative effects on the growth and health of mammals, and has become a factor restricting livestock development at high latitudes and on plateaus. The gut-liver axis is central to energy met... Background Cold stress has negative effects on the growth and health of mammals, and has become a factor restricting livestock development at high latitudes and on plateaus. The gut-liver axis is central to energy metabolism, and the mechanisms by which it regulates host energy metabolism at cold temperatures have rarely been illustrated. In this study, we evaluated the status of glycolipid metabolism and oxidative stress in pigs based on the gut-liver axis and propose that AMP-activated protein kinase(AMPK) is a key target for alleviating energy stress at cold temperatures by dietary fat supplementation.Results Dietary fat supplementation alleviated the negative effects of cold temperatures on growth performance and digestive enzymes, while hormonal homeostasis was also restored. Moreover, cold temperature exposure increased glucose transport in the jejunum. In contrast, we observed abnormalities in lipid metabolism, which was characterized by the accumulation of bile acids in the ileum and plasma. In addition, the results of the ileal metabolomic analysis were consistent with the energy metabolism measurements in the jejunum, and dietary fat supplementation increased the activity of the mitochondrial respiratory chain and lipid metabolism. As the central nexus of energy metabolism, the state of glycolipid metabolism and oxidative stress in the liver are inconsistent with that in the small intestine. Specifically, we found that cold temperature exposure increased glucose transport in the liver, which fully validates the idea that hormones can act on the liver to regulate glucose output. Additionally, dietary fat supplementation inhibited glucose transport and glycolysis, but increased gluconeogenesis, bile acid cycling, and lipid metabolism. Sustained activation of AMPK, which an energy receptor and regulator, leads to oxidative stress and apoptosis in the liver;dietary fat supplementation alleviates energy stress by reducing AMPK phosphorylation.Conclusions Cold stress reduced the growth performance and aggravated glycolipid metabolism disorders and oxidative stress damage in pigs. Dietary fat supplementation improved growth performance and alleviated cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis. In this study, we high-light the importance of AMPK in dietary fat supplementation-mediated alleviation of host energy stress in response to environmental changes. 展开更多
关键词 Apoptosis cold temperature Energy stress FAT Glucolipid metabolism Mitochondrial homeostasis
下载PDF
Genome-Wide Identification of the GST Gene Family in Loquat (Eriobotrya japonica Lindl.) and Their Expression under Cold Stress with ALA Pretreatment
2
作者 Guanpeng Huang Ti Wu +4 位作者 Yinjie Zheng Qiyun Gu Qiaobin Chen Shoukai Lin Jincheng Wu 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第11期2715-2735,共21页
Loquat(Eriobotrya japonica Lindl.),a rare fruit native to China,has a long history of cultivation in China.Low temperature is the key factor restricting loquat growth and severely affects yield.Low temperature induces... Loquat(Eriobotrya japonica Lindl.),a rare fruit native to China,has a long history of cultivation in China.Low temperature is the key factor restricting loquat growth and severely affects yield.Low temperature induces the regeneration and metabolism of reduced glutathione(GSH)to alleviate stress damage via the participation of glu-tathione S-transferases(GSTs)in plants.In this study,16 GSTs were identified from the loquat genome according to their protein sequence similarity with Arabidopsis GSTs.On the basis of domain characteristics and phyloge-netic analysis of AtGSTs,these EjGSTs can be divided into 4 subclasses:Phi,Theta,Tau and Zeta.The basic prop-erties,subcellular localization,structures,motifs,chromosomal distribution and collinearity of the EjGST proteins or genes were further analyzed.Tandem and segmental gene duplications play pivotal roles in EjGST expansion.Cis-elements that respond to various hormones and stresses,especially those associated with low-temperature responsiveness,were predicted to be present in the promoters of EjGSTs.Moreover,analysis of gene expression profiles revealed that 9 of 16 EjGSTs may be involved in the low-temperature responsiveness of loquat leaves.In agriculture,5-aminolevulinic acid(ALA),a potential multifunctional plant growth regulator,can improve the stress response of plants.Among the 9 low-temperature-responsive EjGSTs,the expression of EjGSTU1 and EjGSTF1 significantly differed under cold stress in response to exogenous 5-aminolevulinic acid(ALA)pretreat-ment.The remarkable increase in GST activity and GSH/GSSG ratio reflected the increase in the cold response ability of loquat plants caused by exogenous ALA,thereby alleviating H2O2 accumulation and membrane lipid preoxidation.Overall,this study provides an initial exploration of the cold tolerance function of GSTs in loquat,offering a theoretical foundation for the development of cold-resistant loquat cultivars and new antifreeze agents. 展开更多
关键词 LOQUAT GST gene family identification gene expression cold stress ALA
下载PDF
Effects ofγ-aminobutyric Acid on Nitrogen Metabolism in Roots and Leaves of Cold-stressed Rice(Oryza sativa L.)During Early Vegetative Growth
3
作者 Jia Yan Gong Weibin +9 位作者 Ma Huimiao Liu Ge Zhang Can Liu Aixin Han Yiming Dang Yuxiang Bai Xu Wang Haixing Wu Yulong Xin Junying 《Journal of Northeast Agricultural University(English Edition)》 CAS 2024年第3期1-19,共19页
Cold stress adversely affects rice growth,particularly at the early vegetative growth stage.In higher plants,nitrogen metabolism plays a central role in amino acid metabolism,plant defense mechanisms and productivity.... Cold stress adversely affects rice growth,particularly at the early vegetative growth stage.In higher plants,nitrogen metabolism plays a central role in amino acid metabolism,plant defense mechanisms and productivity.This report investigated the effects of cold stress and supplementalγ-aminobutyric acid(GABA)under cold stress on nitrogen metabolism in rice seedlings.Cold stress resulted in a greater increase in the transformation to NH_(4)^(+)by nitrate reductase(NR)in roots,it further resulted in lower levels of NO_(3)^(-)content in roots,weakened glutamine glutamate(GOGAT/GS)pathway and elevated glutamate dehydrogenase(GDH)pathway of rice seedlings.Whereas,compared with cold stress,supplementation of GABA(2.5 mmol·L^(-1))could increase relative water content(79.43%)and biomass(34.15%)of rice seedlings.GABA could act as an amplifier of stress signal conduction/transduction to increase NR activity and promote NO_(3)^(-)assimilation in leaves.In addition,GABA elicited the Ca^(2+)signaling pathway which could promote the GDH pathway and GABA shunt,increase the activities of GS and GDH,and the expression of OsGAD2 and OsGDH family.The GABA might increase the ratio of the Glu family and avoid NH4+toxicity in order to raise the concentration of organic compounds and alleviate the harmful consequences of cold stress.Based on these observations,this study proposed that GABA mediated cold tolerance in rice seedlings by activating Ca^(2+)burst and subsequent crosstalk among Ca^(2+)signaling,GDH pathway and GABA shunt. 展开更多
关键词 cold stress γ-aminobutyric acid RICE nitrogen metabolism
下载PDF
Effects of stress state on texture and microstructure in cold drawing-bulging of CP-Ti sheet 被引量:3
4
作者 李宏伟 张下陆 +1 位作者 陈甲元 李金山 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第1期23-31,共9页
Three different stress states of the combination of tensile(t) stress and compressive(c) stress,t t,t c and t c c,exist in the deformed commercially pure titanium(CP-Ti) sheet during cold drawing-bulging.The tex... Three different stress states of the combination of tensile(t) stress and compressive(c) stress,t t,t c and t c c,exist in the deformed commercially pure titanium(CP-Ti) sheet during cold drawing-bulging.The textures and microstructures in the different stress state regions were investigated by means of XRD and TEM analysis.Similar development of texture and microstructure is achieved with less thickness strain under multiaxial stresses in drawing-bulging than in cold rolling.The results show that texture and microstructure are much sensitive to multiaxial stresses.Twinning is more easily activated under compressive stress than tensile stress.Prism a slip is heavily affected by tensile stress,resulting in a remarkable change of the intensity of(0°,35°,0°) texture,while pyramidal c+a slip,forming(20°,35°,30°) texture,weakens with the increase of thickness strain in spite of stress state. 展开更多
关键词 titanium alloy TEXTURE twinning MICROSTRUCTURE DRAWING stress state cold rolling drawing-bulging
下载PDF
Study on Change Laws of Cold Tolerance in Wheat under Drought Stress 被引量:3
5
作者 曹文昕 万映秀 +2 位作者 张琪琪 李炎 张平治 《Agricultural Science & Technology》 CAS 2016年第8期1862-1865,1932,共5页
In order to study the change laws of cold tolerance in wheat under drought stress, 11 wheat varieties planted in Huang-Huai wheat area and one variety representative in middle-lower Yangtze River wheat area were selec... In order to study the change laws of cold tolerance in wheat under drought stress, 11 wheat varieties planted in Huang-Huai wheat area and one variety representative in middle-lower Yangtze River wheat area were selected, and the their change laws of cold tolerance under normal moisture condition and drought stress condition. The results showed that under drought condition, the cold tolerance of wheat in various growth stages was remarkably lower than that under nondrought condition, and the decreasing amplitudes were the largest in overwintering stage with an average of 4.91 ℃, the smallest in regreening stage, and in the middle in the jointing stage. Under non-drought condition, the cold tolerance of semi- winter varieties in various growth stages was better than that of spring varieties, there were significant differences between different varieties, and the cold tolerance of all varieties in overwintering stage was improved compared with before winter, and rapidly decreased after overwintering. Semiwinter varieties showed cold tolerance remarkably enhanced in overwintering stage, and the cold tolerance of spring varieties was also improved to a certain degree. 展开更多
关键词 WHEAT Drought stress cold tolerance
下载PDF
Effect of Cold Stress on Some Physiological Indexes of Plants in Malvaceae 被引量:1
6
作者 王玉勤 李健 +2 位作者 田浩人 蒋昌华 胡永红 《Agricultural Science & Technology》 CAS 2010年第11期68-69,123,共3页
The paper was to study the change trend of the physiological indexes in plants of Malvaceae under normal and low temperature condition.The result showed that the increase extent of electrical conductivity of plants wi... The paper was to study the change trend of the physiological indexes in plants of Malvaceae under normal and low temperature condition.The result showed that the increase extent of electrical conductivity of plants with cold tolerance in Malvaceae was significantly lower than the plant without cold tolerance under the cold stress condition,while their proline contents and soluble protein contents were all higher than the plants without cold tolerance.The above physiological indicators all can be used for the screening of varieties with cold tolerance in Malvaceae,which will also provide theoretical guidance for the cold tolerance screening of other garden plants. 展开更多
关键词 MALVACEAE cold stress Physiological indexes
下载PDF
Effects of Reducing Mn^(2+),Cu^(2+) and Zn^(2+) Concentrations in Nutrient Solution on SOD Activity in Grafted Cucumber Seedling Leaves Under Cold Stress
7
作者 李涛 高俊杰 于贤昌 《Agricultural Science & Technology》 CAS 2011年第11期1625-1627,1679,共4页
[Objective] The research aimed to study the effects of reducing the concentrations of Mn2+, Cu2+ or Zn2+ in nutrient solution on the contents of Mn, Cu and Zn of grafted cucumber leaves as well as on the SOD and is... [Objective] The research aimed to study the effects of reducing the concentrations of Mn2+, Cu2+ or Zn2+ in nutrient solution on the contents of Mn, Cu and Zn of grafted cucumber leaves as well as on the SOD and isozymes activities and the rate of electrolytic leakage under cold stress. [Method] Nutrient solution containing low concentrations of Mn2+, Cu2+ or Zn2+ were used for cultivating the grafted cucumber seedlings for the cold treatment. [Result] The results indicated that reducing the concentrations of Mn2+, Cu2+ or Zn2+ in nutrient solution had declined the SOD activities in grafted cucumber leaves and increased the rates of electrolytic leakage. Mn2+ had shown the maximum influences, followed by Cu2+, and Zn2+ had shown the minimum influences. [Conclusion] Cold resistance of cucumber can be improved by changing the contents of Mn2+, Cu2+ or Zn2+ in nutrient solution. 展开更多
关键词 Grafted cucumber Microelement cold stress SOD Rate of electrolytic leakage
下载PDF
Study on the physiological indices of Pinus sibirica and Pinus koraiensis seedlings under cold stress 被引量:13
8
作者 Fang Wang Deyang Liang +7 位作者 Xiaona Pei Qinhui Zhang Peng Zhang Jianqiu Zhang Zhimin Lu Yuchun Yang Guifeng Liu Xiyang Zhao 《Journal of Forestry Research》 SCIE CAS CSCD 2019年第4期1255-1265,共11页
Although Pinus sibirica and Pinus koraiensis are resistant to cold or low temperatures in the cold temperate regions of the northern hemisphere,the former has a stronger cold resistance.Research has been limited to th... Although Pinus sibirica and Pinus koraiensis are resistant to cold or low temperatures in the cold temperate regions of the northern hemisphere,the former has a stronger cold resistance.Research has been limited to the comparison of physiological responses of the two species to cold stress.In this study,5-year-old seedlings of P.sibirica and P.koraiensis were subjected to six temperature treatments,[20℃(control),0℃,-20℃,-40℃,-60℃,and-80℃],under different stress periods(6,12,24,and 48 h).The results showed that differences in each physiological index were significant between P.sibirica and P.koraiensis,except for the permeability of cell membranes,reactive oxygen species,proline and soluble proteins.An ANOVA test indicated that there were extreme differences among the temperatures for each index,stress time and temperature 9 time for most indices.All indices showed a similar trend for P.sibirica and P.koraiensis with decreasing temperature or the extension of stress time.Soluble sugars and proline increased at 0 to-20℃and then remained unchanged with temperature decline.Other indices showed an increase from 20 to-20℃,stable from-20 to-40℃and a decrease from-40 to-80℃.All the indices increased and then declined along with the prolonged cold stress time,except for the control.From 0 to-40℃,the permeability of cell membranes,relative conductivity,reactive oxygen species and malonaldehyde of P.koraiensis seedlings were higher than in P.sibirica,but superoxide dismutase,peroxidase,catalase activity and soluble sugars,proline,and soluble proteins content emerged as opposite.This study compared the physiological mechanism responses to cold stress between P.sibirica and P.koraiensis to provide the basis for the introduction,distribution,and genetic improvement of these coniferous species. 展开更多
关键词 PINUS sibirica P koraiensis cold stress MEMBRANE system ANTIOXIDANT ENZYMES
下载PDF
Application of brassinolide alleviates cold stress at the booting stage of rice 被引量:13
9
作者 WANG Shi-qiang ZHAO Hai-hong +5 位作者 ZHAO Li-ming GU Chun-mei NA Yong-guang XIE Bao-sheng CHENG Shi-hua PAN Guo-jun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第4期975-987,共13页
The objective of the study was to determine the physiological mechanisms of plants in response to brassinolide(BR) alleviating cold water stress on rice. In this study, physiological responses of rice to exogenous BR ... The objective of the study was to determine the physiological mechanisms of plants in response to brassinolide(BR) alleviating cold water stress on rice. In this study, physiological responses of rice to exogenous BR and cold water submergence were investigated using the chilling-tolerant cultivar Kongyu 131(KY131) and the chilling-sensitive cultivar Kenjiandao 6(KJD6). A total of 2 mg L^-1 BR increased activities of superoxide dismutase(SOD) and peroxidase(POD) and the contents of soluble sugar, soluble protein, and chlorophyll, but decreased the malondialdehyde(MDA) content in KY131 and KJD6 under cold water stress. The observed decreases in SOD and POD activities and MDA content recovered quickly after plants were returned to irrigation with water at temperatures of about 23.0°C in 2014. Additionally, the contents of nitrogen(N), phosphorous(P), and potassium(K) were increased by BR treatment under cold water stress. Exposure to BR also raised the percentage of high effective leaf area and leaf area index at the heading stage. Furthermore, it promoted soluble sugar synthesis, increased the rate of dry matter accumulation, and enhanced the export and translocation rates of the stem-sheath. The yield in KJD6 was significantly(P≤0.01 and P≤0.05) higher than that of the control in 2013 and 2014, respectively. The effect of BR treatment on rice leaf SOD and POD activities, MDA, chlorophyll, P, and stem-sheath K contents were more significant in KJD6 than in KY131. In conclusion, exogenous BR effectively reduced the physiological and metabolic damage in rice due to cold stress at the booting stage, promoted functional recovery in plants that received irrigation with water at a normal temperature following cold stress, and mitigated the effects of cold water stress on yield.The two varieties exhibited differential responses to BR;the weaker cold-resistant variety was more sensitive to BR and displayed stronger responses to exogenous BR. 展开更多
关键词 RICE cold WATER stress BRASSINOLIDE PHYSIOLOGICAL characteristics
下载PDF
Physiological and molecular responses to cold stress in rapeseed(Brassica napus L.) 被引量:9
10
作者 YAN Lei Tariq Shah +3 位作者 CHENG Yong Lü Yan ZHANG Xue-kun ZOU Xi-ling 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第12期2742-2752,共11页
Low temperature is one of the most important abiotic factors inhibiting growth, productivity, and distribution of rapeseed(Brassica napus L.). Therefore, it is important to identify and cultivate cold-tolerant germpla... Low temperature is one of the most important abiotic factors inhibiting growth, productivity, and distribution of rapeseed(Brassica napus L.). Therefore, it is important to identify and cultivate cold-tolerant germplasm. The objective of this study was to figure out the mechanism of chilling(4 and 2°C) and freezing(–2 and –4°C) stresses along with a control(22°C) in B. napus cultivars(1801 and C20) under controlled environment(growth chamber). The experiment was arranged in a complete randomized design with three replications. Our results exhibited that under chilling and freezing stresses, the increment of proline accumulation, soluble sugar and protein contents, and antioxidant enzyme activity were enhanced more in 1801 cultivar compared with C20 cultivar. At –2 and –4°C, the seedlings of C20 cultivar died completely compared with 1801 cultivar. Hydrogen peroxide(H2 O2) and malondialdehyde contents(MDA) increased in both cultivars, but when the temperature was decreased up to –2 and –4°C, the MDA and H2 O2 contents continuously dropped in 1801 cultivar. Moreover, we found that leaf abscisic acid(ABA) was enhanced in 1801 cultivar under chilling and freezing stresses. Additionally, the transcriptional regulations of cold-tolerant genes(COLD1, CBF4, COR6.6, COR15, and COR25) were also determined using real-time quantitative PCR(RT-q PCR). RT-q PCR showed that higher expression of these genes were found in 1801 as compared to C20 under cold stress(chilling and freezing stresses). Therefore, it is concluded from this experiment that 1801 cultivar has a higher ability to respond to cold stress(chilling and freezing stresses) by maintaining hormonal, antioxidative, and osmotic activity along with gene transcription process than C20. The result of this study will provide a solid foundation for understanding physiological and molecular mechanisms of cold stress signaling in rapeseed(B. napus). 展开更多
关键词 BRASSICA NAPUS L. cold stress MORPHOLOGICAL features molecular regulation PHYSIOLOGICAL INDICATORS
下载PDF
Effects of Acute and Chronic Cold Stress on Antioxidant Function in Intestinal Tracts of Chickens 被引量:10
11
作者 Xu Ming Yu Xian-yi +3 位作者 Li Jin-long Han Yan-hui Li Shu Xu Shi-wen 《Journal of Northeast Agricultural University(English Edition)》 CAS 2012年第2期54-61,共8页
This study was to investigate the effects of cold stress on the contents of total antioxidant capacity (T-AOC) malondialdehyde (MDA) and superoxide dismutase (SOD) in duodenum, jejunum and ileum of chickens. A t... This study was to investigate the effects of cold stress on the contents of total antioxidant capacity (T-AOC) malondialdehyde (MDA) and superoxide dismutase (SOD) in duodenum, jejunum and ileum of chickens. A total of 80 15-dayold male chickens were treated by cold stress with the duration of the acute cold stress being 1, 3, 6, 12, and 24 h, and the chronic cold stress was 5, 10, and 20 days, respectively. Cold stress temperature was (12±1)℃. The chemical colorimetric method was used to detect the changes of the T-AOC, SOD activities and MDA contents. The results showed that compared with the corresponding control group, effects of acute cold stress on the T-AOC in duodenum, jejunum and ileum of chickens significantly (P〈0.05) increased firstly and then decreased. Under chronic cold stress, the T-AOC significantly (P〈0.05) decreased. Under acute cold stress and chronic cold stress, the MDA contents significantly (P〈0.05) increased in duodenum, jejunum and ileum of chickens. The effects of acute cold stress on the SOD activities in duodenum, jejunum and ileum of chickens significantly (P〈0.05) increased firstly and then decreased Under chronic cold stress the SOD activities significantly (P〈0.05) decreased in jejunum and ileum, but significantly (P〈0.05) decreased firstly and then increased in duodenum. 展开更多
关键词 cold stress INTESTINES CHICKEN ANTIOXIDANT
下载PDF
Seasonal variations of phytoplankton phosphorus stress in the Yellow Sea Cold Water Mass 被引量:7
12
作者 WANG Dan HUANG Bangqin +2 位作者 LIU Xin LIU Guimei WANG Hui 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2014年第10期124-135,共12页
The Yellow Sea is located between the China Mainland and the Korean Peninsula, representing a typical shallow epicontinental sea. The Yellow Sea Cold Water Mass(YSCWM) is one of the most important physical features ... The Yellow Sea is located between the China Mainland and the Korean Peninsula, representing a typical shallow epicontinental sea. The Yellow Sea Cold Water Mass(YSCWM) is one of the most important physical features in the Yellow Sea. The characteristics of vertical profiles and seasonal variations of biogenic elements in the YSCWM may lead the variations of nutrient availability(e.g., phosphorus) and phosphorus stress of phytoplankton. In this study, the authors surveyed the seasonal variations of phytoplankton phosphorus stress with emphasis on the effect of the YSCWM during the four cruises in April and October 2006, March and August 2007. Using both bulk and single-cell alkaline phosphatase activity(APA) assays, this study evaluated phosphorus status of phytoplankton community, succession of phytoplankton community and ecophysiological responses of phytoplankton to phosphorus in the typical region of the YSCWM. With the occurrence of the YSCWM, especially the variations of concentration of dissolved inorganic phosphorus(DIP), the results of bulk APA appeared corresponding seasonal variations. Along Transects A and B, the mean APA in August was the highest, and that in March was the lowest. According to the ELF-labeled assay's results, seasonal variations of the ELF-labeled percentages within dominant species indicated that diatoms were dominant in March, April and October, while dinoflagellates were dominant in August. During the four cruises, the ELF-labeled percentages of diatoms except Paralia sulcata showed that diatoms were not phosphorus deficient in April 2006 at all, but suffered from severe phosphorus stress in August 2007. In comparison, the ELF-labeled percentages of dinoflagellates were all above 50% during the four time series, which meant dinoflagellates such as Alexandrium and Scrippsiella, sustained perennial phosphorus stress. 展开更多
关键词 alkaline phosphatase phosphorus stress PHYTOPLANKTON Yellow Sea cold Water Mass Yellow Sea
下载PDF
Functional characterization of MdMYB73 reveals its involvement in cold stress response in apple calli and Arabidopsis 被引量:5
13
作者 ZHANG Quan-yan YU Jian-qiang +2 位作者 WANG Jia-hui HU Da-gang HAO Yu-jin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第10期2215-2221,共7页
Recent studies have shown that the R2R3-MYB transcription factor MdMYB73 is involved in salt stress response in apple. However, no research was done whether MdMYB73 mediated cold tolerance in apple or not. In this stu... Recent studies have shown that the R2R3-MYB transcription factor MdMYB73 is involved in salt stress response in apple. However, no research was done whether MdMYB73 mediated cold tolerance in apple or not. In this study, we found that the expression of MdMYB73 was obviously induced by cold stress. Functional analysis showed that MdMYB73 significantly increased cold tolerance in transgenic apple calli and Arabidopsis. Quantitative real-time PCR (qRT-PCR) assay indicated that the expression levels of cold-responsive genes including MdCBF2, MdCBF3, MdCBF4, and MdCBF5 were obviously enhanced in MdMYB73 transgenic calli, suggesting that MdMYB73 increased cold tolerance via C-repeat binding factor (CBF) cold response pathway. Finally, we found that soluble sugar, which provides an osmoticum for cells, was increased in MdMYB73 transgenic calli compared to that in the wild type control. These findings provide a new insights into how MdMYB73 is involved in cold stress response. 展开更多
关键词 APPLE MdMYB73 cold stress CBF soluble sugar
下载PDF
Effects of acupuncturing Tsusanli (S_T36) on expression of nitric oxide synthase in hypothalamus and adrenal gland in rats with cold stress ulcer 被引量:10
14
作者 Jin-Ping Sun Hai-Tao Pei +3 位作者 Xiang-Lan Jin Ling Yin Qing-Hua Tian Shu-Jun Tian 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第32期4962-4966,共5页
AIM: To study the protective effect of acupuncturing Tsusanli (ST36) on cold stress ulcer, and the expression of nitric oxide synthase (NOS) in hypothalarnus and adrenal gland. METHODS: Ulcer index in rats and R... AIM: To study the protective effect of acupuncturing Tsusanli (ST36) on cold stress ulcer, and the expression of nitric oxide synthase (NOS) in hypothalarnus and adrenal gland. METHODS: Ulcer index in rats and RT-PCR were used to study the protective effect of acupuncture on cold stress ulcer, and the expression of NOS in hypothalamus and adrenal gland. Images were analyzed with semi-quantitative method. RESULTS: The ulcer index significantly decreased in rats with stress ulcer. Plasma cortisol concentration was up regulated during cold stress, which could be depressed by pre-acupuncture. The expression of NOS1 in hypothallamus increased after acupuncture. The increased expression of NOS2 was related with stress ulcer, which could be decreased by acupuncture. The expression of NOS3 in hypothalamus was similar to NOS2, but the effect of acupuncture was limited. The expression of NOS2 and NOS3 in adrenal gland increased after cold stress, only the expression of NOS1 could be repressed with acupuncture. There was no NOS2 expression in adrenal gland in rats with stress ulcer. CONCLUSION: The protective effect of acupuncturing Tsusanli (ST36) on the expression of NOS in hypothalamus and adrenal gland can be achieved. 展开更多
关键词 ACUPUNCTURE cold stress ULCER RATS NOS
下载PDF
p38 MAPK is a Component of the Signal Transduction Pathway Triggering Cold Stress Response in the MED Cryptic Species of Bemisia tabaci 被引量:6
15
作者 LI Fang-fang XIA Jun +2 位作者 LI Jun-min LIU Shu-sheng WANG Xiao-wei 《Journal of Integrative Agriculture》 SCIE CSCD 2012年第2期303-311,共9页
Cold stress responses help insects to survive under low temperatures that would be lethal otherwise.This phenomenon might contribute to the invasion of some Bemisia tabaci cryptic species from subtropical areas to tem... Cold stress responses help insects to survive under low temperatures that would be lethal otherwise.This phenomenon might contribute to the invasion of some Bemisia tabaci cryptic species from subtropical areas to temperate regions.However,the molecular mechanisms regulating cold stress responses in whitefly are yet unclear.Mitogen-activated protein kinases(MAPKs)which including p38,ERK,and JNK,are well known for their roles in regulating metabolic responses to cold stress in many insects.In this study,we explored the possible roles of the MAPKs in response to low temperature stresses in the Mediterranean cryptic species(the Q-biotype)of the B.tabaci species complex.First,we cloned the p38 and ERK genes from the whitefly cDNA library.Next,we analyzed the activation of MAPKs during cold stress in the Mediterranean cryptic species by immuno-blotting.After cold stress,the level of phospho-p38 increased but no significant change was observed in the phosphorylation of ERK and JNK,thus suggesting that the p38 might be responsible for the defense response to low temperature stress.Furthermore,we demonstrated that:i)3 min chilling at 0°C was sufficient for the activation of p38 MAPK pathway in this whitefly;and ii)the amount of phosphorylated p38 increased significantly in the first 20 min of chilling,reversed by 60 min,and then returned to the original level by 120 min.Taken together,our results suggest that the p38 pathway is important during response to low temperature stress in the Mediterranean cryptic species of the B.tabaci species complex. 展开更多
关键词 Bemisia tabaci cold stress MAPK P38 PHOSPHORYLATION signal transduction
下载PDF
Investigation of the short-term stress distribution in stopes and drifts backfilled with cemented paste backfill 被引量:7
16
作者 Pengyu Yang Li Li 《International Journal of Mining Science and Technology》 SCIE EI CSCD 2015年第5期721-728,共8页
Cemented paste backfill(CPB) is largely used in underground mines worldwide.A key issue associated with application of CPB is to estimate the stresses in backfilled stopes and on barricades.Recent numerical and experi... Cemented paste backfill(CPB) is largely used in underground mines worldwide.A key issue associated with application of CPB is to estimate the stresses in backfilled stopes and on barricades.Recent numerical and experimental results show that arching effect is absent shortly after the placement of CPB in stopes.However,stress decreases in barricade drift with increasing distance between the measurement points and drawpoint have also been observed,demonstrating arching effect shortly after the pouring of CPB.To explain these paradoxes,CPB is considered as Bingham fluid having a yield shear stress.Three dimensional analytical solutions are proposed to evaluate the short-term total stresses in backfilled stopes and on barricades,accounting for the CPB's yield shear stress-induced arching effect.Stress diminution due to such arching effect in the backfilled stopes and on barricades is indeed obtained.But the reduction becomes insignificant using typical yield shear stress and stope geometry.More analyses indicate that the typical yield shear stress values do not fully correspond to field conditions where the yield shear stress would increase exponentially due to apparent consolidation(loss of water by drainage,a phenomenon similar to the desiccation of overly saturated fine-grained materials). 展开更多
关键词 Mines Cemented paste backfill Yield shear stress short-term total stresses Arching effect Analytical solutions
下载PDF
Effects of cold plasma treatment on alfalfa seed growth under simulated drought stress 被引量:4
17
作者 冯金奎 王德成 +2 位作者 邵长勇 张丽丽 唐欣 《Plasma Science and Technology》 SCIE EI CAS CSCD 2018年第3期129-135,共7页
The effect of different cold plasma treatments on the germination and seedling growth of alfalfa(Medicago sativa L.) seeds under simulated drought stress conditions was investigated.Polyethyleneglycol-6000(PEG 6000... The effect of different cold plasma treatments on the germination and seedling growth of alfalfa(Medicago sativa L.) seeds under simulated drought stress conditions was investigated.Polyethyleneglycol-6000(PEG 6000)with the mass fraction of 0%(purified water), 5%, 10%,and 15% were applied to simulate the drought environment. The alfalfa seeds were treated with15 different power levels ranged between 0–280 W for 15 s. The germination potential,germination rate, germination index, seedling root length, seedling height, and vigor index were investigated. Results indicated significant differences between treated with proper power and untreated alfalfa seeds. With the increase of treatment power, these indexes mentioned above almost presented bimodal curves. Under the different mass fractions of PEG 6000, results showed that the lower power led to increased germination, and the seedlings presented good adaptability to different drought conditions. Meanwhile, higher power levels resulted in a decreased germination rate. Seeds treated with 40 W resulted in higher germination potential,germination rate, seedling height, root length, and vigor index. Vigor indexes of the treated seeds under different PEG 6000 stresses increased by 38.68%, 43.91%, 74.34%, and 39.20%respectively compared to CK_(0-0), CK_(5-0), CK_(10-0), and CK_(15-0)(the control sample under 0%, 5%,10%, and 15% PEG 6000). Therefore, 40 W was regarded as the best treatment in this research.Although the trend indexes of alfalfa seeds treated with the same power were statistically the same under different PEG 6000 stresses, the cold plasma treatment had a significant effect on the adaptability of alfalfa seeds in different drought environments. Thus, this kind of treatment is worth implementing to promote seed growth under drought situations. 展开更多
关键词 cold plasma ALFALFA PEG 6000 stress GERMINATION SEEDLING plant growth
下载PDF
Genetic Control of Germination Ability under Cold Stress in Rice 被引量:4
18
作者 WANG Zhou-fei WANG Jian-fei +3 位作者 WANG Fu-hua BAO Yong-mei Wu Yun-yu ZHANG Hong-sheng 《Rice science》 SCIE 2009年第3期173-180,共8页
An F9 recombinant inbred lines (RIL) population, derived from a cross between IR28 (Oryza sativa L. spp. indica) and Daguandao (O. sativa L. spp. japonica), was used to construct a molecular linkage map and to i... An F9 recombinant inbred lines (RIL) population, derived from a cross between IR28 (Oryza sativa L. spp. indica) and Daguandao (O. sativa L. spp. japonica), was used to construct a molecular linkage map and to identify germination ability including the traits of imbibition rate, germination rate, germination index, root length, shoot length and seed vigor at 14℃ for 23 d. A composite interval mapping approach was applied to conduct genetic analysis for germination ability. The frequency distributions of the germination ability traits under the cold stress in the RIL population showed continuous segregation, suggesting they were quantitative traits controlled by several genes. A total of seven QTLs were identified on chromosomes 4 6 and 9, including two for imbibition rate (qIR-6, qIR-9), one for germination rate (qGR-4), two for germination index (qGI-4-1, qGI-4-2) and two for root length (qRL-4-1, qRL-4-2). There were no detected QTLs controlling shoot length and seed vigor. The phenotypic variance explained by a single QTL ranged from 9.1% to 37.0%, and two major QTLs, qlR-6 and qGI-4-2, accounted for over 30% of the phenotypic variance. The expressions of QTLs were developmentally regulated and growth stage-specific. Most of the QTLs observed here were located in the regions similar to the QTLs for rice cold tolerance reported previously, indicating that these QTLs were reliable. However, qRL-4-2 is not reported before. 展开更多
关键词 RICE cold stress germination ability quantitative trait locus
下载PDF
Comparison of transcriptome and metabolome analysis revealed differences in cold resistant metabolic pathways in different apple cultivars under low temperature stress 被引量:6
19
作者 Gongxun Xu Lijie Li +3 位作者 Jia Zhou Deguo Lyu Deying Zhao Sijun Qin 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第2期183-198,共16页
Freezing injury in winter is an important abiotic stress that seriously affects plant growth and development.Deciduous fruit trees resist freezing injury by inducing dormancy.However,different cultivars of the same sp... Freezing injury in winter is an important abiotic stress that seriously affects plant growth and development.Deciduous fruit trees resist freezing injury by inducing dormancy.However,different cultivars of the same species have different cold resistance strategies.Little is known about the molecular mechanism of apple trees in response to freezing injury during winter dormancy.Therefore,in this study,1-year-old branches of the cold-resistant cultivar‘Hanfu’(HF)and the cold-sensitive cultivar‘Changfuji No.2’(CF)were used to explore their cold resistance through physiological,biochemical,transcriptomics,and metabolomics analyses.Combining physiological and biochemical data,we found that HF had a stronger osmotic regulation ability and antioxidant enzyme activity than CF,as well as stronger cold resistance.The functional enrichment analysis showed that both cultivars were significantly enriched in pathways related to signal transduction,hormone regulation,and sugar metabolism under freezing stress.In addition,the differentially expressed genes(DEGs)encoding galactinol synthase,raffinose synthase,and stachyose synthetase in raffinose family oligosaccharides(RFOs)metabolic pathways were upregulated in HF,and raffinose and stachyose were accumulated,while their contents in CF were lower.HF accumulated 4-aminobutyric acid,spermidine,and ascorbic acid to scavenge reactive oxygen species(ROS).While the contents of oxidized glutathione,vitamin C,glutathione,and spermidine in CF decreased under freezing stress,consequently,the ability to scavenge ROS was low.Furthermore,the transcription factors apetala 2/ethylene responsive factor(AP2/ERF)and WRKY were strongly induced under freezing stress.In summary,the difference in key metabolic components of HF and CF under freezing stress is the major factor affecting their difference in cold resistance.The obtained results deepen our understanding of the cold resistance mechanism in apple trees in response to freezing injury during dormancy. 展开更多
关键词 APPLE Freezing stress cold resistance Galactinol synthase TRANSCRIPTOME METABOLOME
下载PDF
Cold Stress-induced Glucosyltransferase CsUGT78A15 is Involved in the Formation of Eugenol Glucoside in Camellia sinensis 被引量:7
20
作者 Mingyue Zhao Binbin Cai +12 位作者 Jieyang Jin Na Zhang Tingting Jing Jingming Wang Yuting Pan Zixiang Zhou Yifan Zhao Yingying Feng Feng Yu Mengting Zhang Yating Li Zhonghua Liu Chuankui Song 《Horticultural Plant Journal》 SCIE 2020年第6期439-449,共11页
Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributi... Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality.However,the details of their biosynthesis and metabolism in tea plants are still unknown.Here,eugenol glucoside was unambiguously identified as a native metabolite in the tea plant,and its biosynthesis was shown to be induced by low temperature treatment.Through the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78A15 was identified in tea,and its encoded protein was shown to catalyze the glucosylation of eugenol.Vmax/Km ratios showed that eugenol was the most suitable substrate for CsUGT78A15.Sugar donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose,followed by galactose and glucuronic acid.The expression of CsUGT78A15was correlatedwith the accumulation of eugenol glucoside in different tissues and genotypes of tea.Down-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress,indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress.The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside. 展开更多
关键词 Camellia sinensis EUGENOL GLUCOSYLTRANSFERASE cold stress
下载PDF
上一页 1 2 126 下一页 到第
使用帮助 返回顶部