Objective : To investigate the effect of Huan shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor β 2 (TGF β 2 ) in the remnant kidney of rats. Methods : Male Wistar ra...Objective : To investigate the effect of Huan shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor β 2 (TGF β 2 ) in the remnant kidney of rats. Methods : Male Wistar rats, after 4 weeks of 5/6 nephrectomy, were divided into two groups at random, and treated with water and Huanshuai Recipe (HSR) respectively. Serum creatinine (SCr) and urinary protein of 24 hours were observed at beginning, 6th week and 12th week of the experiment, and FN and TGF β 2 were assayed by immunohistochemical technique and computer assisted image analysis as well. Results : Serum SCr and urinary protein of 24 hours were increased significantly in the animals after 5/6 nephrectomy. The increased parameters decreased in the HSR group after treatment, as compared with those in the control group, and the difference was significant ( P <0.05 ); Immunohistochemical measurement and computer assisted image analysis showed a marked suppression of FN and TGF β 2 in animals of the HSR group with significant difference from those in the control group ( P <0.05). Conclusion : HSR not only plays the role of renal function protection, but could also alleviate the glomerular pathological injury. The effect on TGF β 2 and FN may be the mechanism of HSR in slowing down the progression of chronic renal failure.展开更多
文摘Objective : To investigate the effect of Huan shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor β 2 (TGF β 2 ) in the remnant kidney of rats. Methods : Male Wistar rats, after 4 weeks of 5/6 nephrectomy, were divided into two groups at random, and treated with water and Huanshuai Recipe (HSR) respectively. Serum creatinine (SCr) and urinary protein of 24 hours were observed at beginning, 6th week and 12th week of the experiment, and FN and TGF β 2 were assayed by immunohistochemical technique and computer assisted image analysis as well. Results : Serum SCr and urinary protein of 24 hours were increased significantly in the animals after 5/6 nephrectomy. The increased parameters decreased in the HSR group after treatment, as compared with those in the control group, and the difference was significant ( P <0.05 ); Immunohistochemical measurement and computer assisted image analysis showed a marked suppression of FN and TGF β 2 in animals of the HSR group with significant difference from those in the control group ( P <0.05). Conclusion : HSR not only plays the role of renal function protection, but could also alleviate the glomerular pathological injury. The effect on TGF β 2 and FN may be the mechanism of HSR in slowing down the progression of chronic renal failure.
