Aim To investigate the chemical composition of Ranunculus sieboldii Miq..Methods Repeated column chromatography over silica gel, polyamide and RP-18 followed by gelfiltration on sephadex LH-20 were used to isolate che...Aim To investigate the chemical composition of Ranunculus sieboldii Miq..Methods Repeated column chromatography over silica gel, polyamide and RP-18 followed by gelfiltration on sephadex LH-20 were used to isolate chemical constituents, and their structures wereelucidated by extensive spectroscopic methods (UV, IR, MS, ~1H NMR, ^(13)C NMR) including 2D NMR(COSY, HMQC, HMBC, NOESY) techniques and by direct comparing spectral data with those reported inliterature. Results Five flavonoid glycosides named apigenin-4'-O-α-L-rhamnopyranoside (1),apigenin-7-O-β-D-glucopyranosyl-4'-O-α-L-rhamnopyranoside (2), apigenin-8-C-α-L-arabinopyranoside(3), apigenin-8-C-β-D-ga-lactopyranoside (4) , tricin-7-O-β-D-glucopyranoside (5), together withtricin (6), luteolin (7), scopoletin (8), esculetin (9), scoparone (10), ferulic acid (11),protocatechuic acid (12) , and tematolide (13) were isolated from the 95% etha-nolic extract of itswhole plant, and their cytotoxic activities were preliminarily tested. Conclusion Compounds 1-12were obtained from this genus and compound 13 from this species for the first time. Furthermore,compound 1 was for the first time isolated from nature while the ^(13)C NMR data of compounds 2 and3 are reported for the first time. The bioassay revealed that compound 1 was active against BEL-7407and A549 cell lines (IC_(45) 43, 77 μg·mL^(-1)), 8 and 10 showed inhibitory activities on KB celllines (IC_(50) 78, 44 μg·mL^(-1)) and HL-60 cell lines (Ic_(50) 85, 85 μg·mL^(-1)), while 7exerted moderate cytotoxic activities on KB, BFL-7407, A549 and HL-60 cell lines with their IC_(50)being 51, 55, 44 and 10 μg·mL^(-1) , respectively.展开更多
[Objective] The aim of this study was to investigate spontaneous self- pollination mechanism of Asarum sieboldii. [Methed] Through field observation and manual controlling test under the cultivated condition, the whol...[Objective] The aim of this study was to investigate spontaneous self- pollination mechanism of Asarum sieboldii. [Methed] Through field observation and manual controlling test under the cultivated condition, the whole flowering process of Asarum sieboldii belonging to Asarum subgenus Heterotropa was thoroughly ob- served to investigate its pollination mechanism. [Result] Asarum sieboldii is a kind of species dominated by spontaneous self-pollination. Its female flower became mature firstly, and the duration of single flower ranged from 3 to 4 d. The first day was fe- male phase duration, and the stigmas could receive foreign pollen, while stamen fila- ments bent down towards the base of calyx tube with 90°, and anther had no crack. During the following 2 days, two cycles of stamens erected subsequently, while an- thers became dehiscent and naturally contacted with stigma at the same time, so spontaneous self-pollination was finished. Artificial self-pollination and out-crossing test had proved that the out-crossing of Asarum sieboldii were all fertile with seed setting rate of 100%. Emasculation by bagging test had suggested that Asarum sieboldii had no parthenocarpy and apomixes. [Conclusion] Asarum sieboldii belongs to sub- genus Heterotropa, but its pollination mechanism is similar to that of subgenus Asarum.展开更多
Magnoline（1）,a new isoquinoline derivative,was isolated from the leaves of Magnolia sieboldii K.Koch（Magnoliaceae）.Its structure was elucidated on the basis of spectral analysis including 1D,2D NMR and HR-TOF-MS.
Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic...Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic DNA oxidation in HT-1080 cells. Methods: The ROS generation induced by H2 O2 was measured by the dichlorofluorescein-diacetate assay. GSH levels were measured using a fluorescent method with mBBr. Genomic DNA oxidative damage was measured with levels of oxidative DNA induced by the reaction of ferritin with H2 O2. Results: The n-hexane, 85% aqueous methanol and n-butanol fractions(0.05 mg/mL concentrations) inhibited H2 O2-induced ROS generation by 63%, 35% and 45%, respectively. GSH levels were significantly increased in both acetone+methylene chloride and methanol extracts(P<0.05). Supplementation of cells with n-hexane significantly increased GSH levels at concentrations of 0.05 mg/mL(P<0.05). Both the acetone+methylene chloride and methanol extracts, as well as all fractions significantly inhibited oxidative DNA damage(P<0.05). Conclusions: These results indicate that cellular oxidation was inhibited by the n-hexane fraction and this fraction may contain valuable active compounds.展开更多
Magnolia sieboldii K. Koch seed is characterized with having deep dormancy. The inner molecular regulation mechanism has not been investigated because of the absence of a protocol for total RNA(ribonucleic acid)extrac...Magnolia sieboldii K. Koch seed is characterized with having deep dormancy. The inner molecular regulation mechanism has not been investigated because of the absence of a protocol for total RNA(ribonucleic acid)extraction. The extraction of high-quality RNA is important and can be a limiting factor in plant molecular biology experiments. Sufficient high-quality total RNA is required to elucidate the molecular regulation mechanism of germination. However, M. sieboldii seeds with large amounts of secondary metabolites also contain recalcitrant tissues for RNA isolation. We found two simple and low-cost RNA extraction methods for M. sieboldii seeds by evaluating and selecting eight types of methods and further optimizing these methods. The two methods were not only suitable for extracting M. sieboldii seed RNA but also applicable to RNAs from several other tissues. Total RNA extracted through these approaches was applicable for general molecular biology experiments such as qRT-PCR(quantitative real-time polymerase chain reactions). The protocols also meet the strict harsh requirements for transcriptome sequencing and small RNA sequencing. This study provides a powerful approach for future studies at the transcription level.展开更多
收集Web of Science^(TM)和CNKI文献检索数据库中的天女木兰资料,利用Citespace可视化文献计量工具分析1980—2023年天女木兰研究领域的202篇有效文献,根据关键词共现和关键词聚类时间轴知识图谱,总结当前天女木兰的热门研究话题——繁...收集Web of Science^(TM)和CNKI文献检索数据库中的天女木兰资料,利用Citespace可视化文献计量工具分析1980—2023年天女木兰研究领域的202篇有效文献,根据关键词共现和关键词聚类时间轴知识图谱,总结当前天女木兰的热门研究话题——繁育手段、种子休眠解除机制、药用价值、开花生物学特性和抗寒机制.其中,种子变温层积催芽技术是天女木兰种子繁殖研究的主要内容,而无性繁殖技术的研究则侧重于筛选能够获得最佳繁殖效果的处理方法;关键基因鉴定是种子休眠解除机制的探索重点;精油成分功效以及木香烃内酯导致细胞凋亡的途径是天女木兰药用价值的研究热点;通过了解天女木兰开花生物学特性研究濒危树种保育对策;抗寒机制的研究集中于抗逆生理响应.制订科学的繁殖技术方案,加强生态适应机制和适应能力研究,保护与开发有机结合应成为今后天女木兰研究的主攻方向.展开更多
文摘Aim To investigate the chemical composition of Ranunculus sieboldii Miq..Methods Repeated column chromatography over silica gel, polyamide and RP-18 followed by gelfiltration on sephadex LH-20 were used to isolate chemical constituents, and their structures wereelucidated by extensive spectroscopic methods (UV, IR, MS, ~1H NMR, ^(13)C NMR) including 2D NMR(COSY, HMQC, HMBC, NOESY) techniques and by direct comparing spectral data with those reported inliterature. Results Five flavonoid glycosides named apigenin-4'-O-α-L-rhamnopyranoside (1),apigenin-7-O-β-D-glucopyranosyl-4'-O-α-L-rhamnopyranoside (2), apigenin-8-C-α-L-arabinopyranoside(3), apigenin-8-C-β-D-ga-lactopyranoside (4) , tricin-7-O-β-D-glucopyranoside (5), together withtricin (6), luteolin (7), scopoletin (8), esculetin (9), scoparone (10), ferulic acid (11),protocatechuic acid (12) , and tematolide (13) were isolated from the 95% etha-nolic extract of itswhole plant, and their cytotoxic activities were preliminarily tested. Conclusion Compounds 1-12were obtained from this genus and compound 13 from this species for the first time. Furthermore,compound 1 was for the first time isolated from nature while the ^(13)C NMR data of compounds 2 and3 are reported for the first time. The bioassay revealed that compound 1 was active against BEL-7407and A549 cell lines (IC_(45) 43, 77 μg·mL^(-1)), 8 and 10 showed inhibitory activities on KB celllines (IC_(50) 78, 44 μg·mL^(-1)) and HL-60 cell lines (Ic_(50) 85, 85 μg·mL^(-1)), while 7exerted moderate cytotoxic activities on KB, BFL-7407, A549 and HL-60 cell lines with their IC_(50)being 51, 55, 44 and 10 μg·mL^(-1) , respectively.
基金Supported by Scientific Research Plan from Shaanxi Provincial Education Department(03jk095)~~
文摘[Objective] The aim of this study was to investigate spontaneous self- pollination mechanism of Asarum sieboldii. [Methed] Through field observation and manual controlling test under the cultivated condition, the whole flowering process of Asarum sieboldii belonging to Asarum subgenus Heterotropa was thoroughly ob- served to investigate its pollination mechanism. [Result] Asarum sieboldii is a kind of species dominated by spontaneous self-pollination. Its female flower became mature firstly, and the duration of single flower ranged from 3 to 4 d. The first day was fe- male phase duration, and the stigmas could receive foreign pollen, while stamen fila- ments bent down towards the base of calyx tube with 90°, and anther had no crack. During the following 2 days, two cycles of stamens erected subsequently, while an- thers became dehiscent and naturally contacted with stigma at the same time, so spontaneous self-pollination was finished. Artificial self-pollination and out-crossing test had proved that the out-crossing of Asarum sieboldii were all fertile with seed setting rate of 100%. Emasculation by bagging test had suggested that Asarum sieboldii had no parthenocarpy and apomixes. [Conclusion] Asarum sieboldii belongs to sub- genus Heterotropa, but its pollination mechanism is similar to that of subgenus Asarum.
文摘Magnoline（1）,a new isoquinoline derivative,was isolated from the leaves of Magnolia sieboldii K.Koch（Magnoliaceae）.Its structure was elucidated on the basis of spectral analysis including 1D,2D NMR and HR-TOF-MS.
基金supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2017R1A2B4005915)
文摘Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic DNA oxidation in HT-1080 cells. Methods: The ROS generation induced by H2 O2 was measured by the dichlorofluorescein-diacetate assay. GSH levels were measured using a fluorescent method with mBBr. Genomic DNA oxidative damage was measured with levels of oxidative DNA induced by the reaction of ferritin with H2 O2. Results: The n-hexane, 85% aqueous methanol and n-butanol fractions(0.05 mg/mL concentrations) inhibited H2 O2-induced ROS generation by 63%, 35% and 45%, respectively. GSH levels were significantly increased in both acetone+methylene chloride and methanol extracts(P<0.05). Supplementation of cells with n-hexane significantly increased GSH levels at concentrations of 0.05 mg/mL(P<0.05). Both the acetone+methylene chloride and methanol extracts, as well as all fractions significantly inhibited oxidative DNA damage(P<0.05). Conclusions: These results indicate that cellular oxidation was inhibited by the n-hexane fraction and this fraction may contain valuable active compounds.
基金supported by the National Natural Science Foundation of China(No.31570621)
文摘Magnolia sieboldii K. Koch seed is characterized with having deep dormancy. The inner molecular regulation mechanism has not been investigated because of the absence of a protocol for total RNA(ribonucleic acid)extraction. The extraction of high-quality RNA is important and can be a limiting factor in plant molecular biology experiments. Sufficient high-quality total RNA is required to elucidate the molecular regulation mechanism of germination. However, M. sieboldii seeds with large amounts of secondary metabolites also contain recalcitrant tissues for RNA isolation. We found two simple and low-cost RNA extraction methods for M. sieboldii seeds by evaluating and selecting eight types of methods and further optimizing these methods. The two methods were not only suitable for extracting M. sieboldii seed RNA but also applicable to RNAs from several other tissues. Total RNA extracted through these approaches was applicable for general molecular biology experiments such as qRT-PCR(quantitative real-time polymerase chain reactions). The protocols also meet the strict harsh requirements for transcriptome sequencing and small RNA sequencing. This study provides a powerful approach for future studies at the transcription level.
文摘收集Web of Science^(TM)和CNKI文献检索数据库中的天女木兰资料,利用Citespace可视化文献计量工具分析1980—2023年天女木兰研究领域的202篇有效文献,根据关键词共现和关键词聚类时间轴知识图谱,总结当前天女木兰的热门研究话题——繁育手段、种子休眠解除机制、药用价值、开花生物学特性和抗寒机制.其中,种子变温层积催芽技术是天女木兰种子繁殖研究的主要内容,而无性繁殖技术的研究则侧重于筛选能够获得最佳繁殖效果的处理方法;关键基因鉴定是种子休眠解除机制的探索重点;精油成分功效以及木香烃内酯导致细胞凋亡的途径是天女木兰药用价值的研究热点;通过了解天女木兰开花生物学特性研究濒危树种保育对策;抗寒机制的研究集中于抗逆生理响应.制订科学的繁殖技术方案,加强生态适应机制和适应能力研究,保护与开发有机结合应成为今后天女木兰研究的主攻方向.
