Single cell trapping in vitro by microfluidic device is an emerging approach for the study of the relationship between single cells and their dynamic biochemical microenvironments. In this paper, a hydrodynamic-based ...Single cell trapping in vitro by microfluidic device is an emerging approach for the study of the relationship between single cells and their dynamic biochemical microenvironments. In this paper, a hydrodynamic-based microfluidic device for single cell trapping is designed using a combination of stagnation point flow and physical barrier.The microfluidic device overcomes the weakness of the traditional ones, which have been only based upon either stagnation point flows or physical barriers, and can conveniently load dynamic biochemical signals to the trapped cell. In addition, it can connect with a programmable syringe pump and a microscope to constitute an integrated experimental system.It is experimentally verified that the microfluidic system can trap single cells in vitro even under flow disturbance and conveniently load biochemical signals to the trapped cell. The designed micro-device would provide a simple yet effective experimental platform for further study of the interactions between single cells and their microenvironments.展开更多
Analyzed the relation between time delay difference and time delay estimation errors, based on the principles of three-point locating, a reformed threshold method for time delay difference estimation of impulse signal...Analyzed the relation between time delay difference and time delay estimation errors, based on the principles of three-point locating, a reformed threshold method for time delay difference estimation of impulse signals, called as amendment estimation for short, is developed by introducing channel equalization technique to its conventional version, named as direct estimation in this paper, to improve the estimation stability. After inherent relationship between time delay and phase shift of signals is analyzed, an integer period error compensation method utilized the diversities of both contribution share and contribution mode of concerned estimates is proposed under the condition of high precision phase lag estimation. Finally, a cooperative multi-threshold estimation method composed of amendment and direct estimations to process impulse signals with three thresholds is established. In sea trials data tests of passive locating, this method improves the estimation precision of time delay difference efficiently. The experiments verify the theoretical predictions.展开更多
A point-of-care test system has been studied in this paper.It was used to determine substances in blood such as Hemoglobin (HB),Aspartate Aminotransferase (AST),Creatine Kinase (CK) and so on.Based on the principle ...A point-of-care test system has been studied in this paper.It was used to determine substances in blood such as Hemoglobin (HB),Aspartate Aminotransferase (AST),Creatine Kinase (CK) and so on.Based on the principle of amperometric determination,the research on detecting weak current signals was carried on.At the same time as to the weak signals (nA level),magnifying,sampling and processing the signals were also studied.Controlled by ADUC824 and assisted by other units, every substance could be determined automatically and rapidly integrated with the corresponding biosensor.In the experiment, the minimum detectable current of the instrument (YT2005-1) is 0.2 nA.With regard to the 1 nA which the experiment demanded,it could be up to the mustard.And the system can provide results in 180 s with a long term stability.展开更多
A Wi-Fi fingerprinting localization approach has attracted increasing attention in recent years due to the ubiquity of Access Point( AP). However,typical fingerprinting localization methods fail to resist accidental e...A Wi-Fi fingerprinting localization approach has attracted increasing attention in recent years due to the ubiquity of Access Point( AP). However,typical fingerprinting localization methods fail to resist accidental environmental changes,such as AP movement. In order to address this problem,a robust fingerprinting indoor localization method is initiated. In the offline phase,three attributes of Received Signal Strength Indication( RSSI) —average,standard deviation and AP's response rate—are computed to prepare for the subsequent computation. In this way,the underlying location-relevant information can be captured comprehensively. Then in the online phase, a three-step voting scheme-based decision mechanism is demonstrated, detecting and eliminating the part of AP where the signals measured are severely distorted by AP 's movement. In the following localization step,in order to achieve accuracy and efficiency simultaneously,a novel fingerprinting localization algorithm is applied. Bhattacharyya distance is utilized to measure the RSSI distribution distance,thus realizing the optimization of MAximum Overlapping algorithm( MAO). Finally,experimental results are displayed,which demonstrate the effectiveness of our proposed methods in eliminating outliers and attaining relatively higher localization accuracy.展开更多
In this paper, conditions for the existence of equilibrium points and global stability are emphatically discussed for Bidirectional Associative Memory(BAM) models with axonal signal transmission delays, and the discus...In this paper, conditions for the existence of equilibrium points and global stability are emphatically discussed for Bidirectional Associative Memory(BAM) models with axonal signal transmission delays, and the discussed methods are more general. The correctness of obtained conclusions is verified by use of some examples. The obtained results have primary significance in the design and application of BAM.展开更多
Here, we administered repeated-pulse transcranial magnetic stimulation to healthy people at the left Guangming （GB37） and a mock point, and calculated the sample entropy of electroencephalo- gram signals using nonli...Here, we administered repeated-pulse transcranial magnetic stimulation to healthy people at the left Guangming （GB37） and a mock point, and calculated the sample entropy of electroencephalo- gram signals using nonlinear dynamics. Additionally, we compared electroencephalogram sample entropy of signals in response to visual stimulation before, during, and after repeated-pulse tran- scranial magnetic stimulation at the Guangming. Results showed that electroencephalogram sample entropy at left （F3） and right （FP2） frontal electrodes were significantly different depending on where the magnetic stimulation was administered. Additionally, compared with the mock point, electroencephalogram sample entropy was higher after stimulating the Guangming point. When visual stimulation at Guangming was given before repeated-pulse transcranial magnetic stimula- tion, significant differences in sample entropy were found at five electrodes （C3, Cz, C4, P3, T8） in parietal cortex, the central gyrus, and the right temporal region compared with when it was given after repeated-pulse transcranial magnetic stimulation, indicating that repeated-pulse transcranial magnetic stimulation at Guangming can affect visual function. Analysis of electroencephalogram revealed that when visual stimulation preceded repeated pulse transcranial magnetic stimulation, sample entropy values were higher at the C3, C4, and P3 electrodes and lower at the Cz and T8 electrodes than visual stimulation followed preceded repeated pulse transcranial magnetic stimula- tion. The findings indicate that repeated-pulse transcranial magnetic stimulation at the Guangming evokes different patterns of electroencephalogram signals than repeated-pulse transcranial mag- netic stimulation at other nearby points on the body surface, and that repeated-pulse transcranial magnetic stimulation at the Guangrning is associated with changes in the complexity of visually evoked electroencephalogram signals in parietal regions, central gyrus, and temporal regions.展开更多
为了提高量子数字信号表示的灵活性,本文提出了一种新的基于补码浮点表示的一维有限长度量子数字信号表示模型(Complement Floating-point Representation of Digital Signals,CFRDS)。该模型使用两组量子比特序列分别表示位置信息与幅...为了提高量子数字信号表示的灵活性,本文提出了一种新的基于补码浮点表示的一维有限长度量子数字信号表示模型(Complement Floating-point Representation of Digital Signals,CFRDS)。该模型使用两组量子比特序列分别表示位置信息与幅值信息。其中,位置信息采用有符号定点整数补码形式表示,保证了信号位置的精确性和负数处理能力;幅值信息则采用浮点数形式表示,浮点数的阶码和尾数均采用补码形式,能够更灵活地应对不同幅值的信号,确保在极端数值条件下依然保持高精度,同时,这种表示方法也简化了数学运算,能够处理更广泛的信号类型。该模型不仅在信号幅值的表示范围与精度上取得了显著提升,还在数学运算的便捷性方面展现了优越性,使得各种信号处理算法更加高效和可靠,适用于更加复杂的信号处理算法,提高了信号处理的效率。本文提出了CFRDS模型,设计了该模型的量子制备线路与基于该模型的量子数字信号基本运算线路,包括两个量子数字信号的序列加法、序列乘法以及自相关函数序列运算,并深入分析了线路复杂度,最后通过计算机仿真实验验证了所提出方案的可行性和有效性。展开更多
点云的处理、传输、语义分割等是3维计算机视觉领域重要的分析任务.现如今,图神经网络和图结构在点云研究方面的有效性已被证实,基于图的点云(graph-based point cloud,GPC)研究不断涌现.因此,一种统一的研究角度、框架和方法论亟待形成...点云的处理、传输、语义分割等是3维计算机视觉领域重要的分析任务.现如今,图神经网络和图结构在点云研究方面的有效性已被证实,基于图的点云(graph-based point cloud,GPC)研究不断涌现.因此,一种统一的研究角度、框架和方法论亟待形成.系统性梳理了GPC研究的各种应用场景,包括配准、降噪、压缩、表示学习、分类、分割、检测等任务,概括出GPC研究的一般性框架,提出了一条覆盖当前GPC全域研究的技术路线.具体来说,给出了GPC研究的分层概念范畴,包括底层数据处理、中层表示学习、高层识别任务;综述了各领域中的GPC模型或算法,包括静态和动态点云的处理算法、有监督和无监督的表示学习模型、传统或机器学习的GPC识别算法;总结了其中代表性的成果及其核心思想,譬如动态更新每层特征空间对应的最近邻图、分层以及参数共享的动态点聚合模块,结合图划分和图卷积提高分割精度;对比了模型性能,包括总体精度(overall accuracy,OA)、平均精度(mean accuracy,mAcc)、平均交并比(mean intersection over union,mIoU);在分析比较现有模型和方法的基础上,归纳了GPC目前面临的主要挑战,提出相应的研究问题,并展望未来的研究方向.建立的GPC研究框架具有一般性和通用性,为后续研究者从事GPC这个新型交叉领域研究提供了领域定位、技术总结及宏观视角.点云研究的出现,是探测器硬件技术长足进步后应运而生的结果;点云研究的现状表明在理论和实践之间存在一些挑战,一些关键问题还有待解决.同时,点云研究的发展将推动人工智能进入新的时代.展开更多
基金supported by the National Natural Science Foundation of China (Grants 11172060 and 31370948)
文摘Single cell trapping in vitro by microfluidic device is an emerging approach for the study of the relationship between single cells and their dynamic biochemical microenvironments. In this paper, a hydrodynamic-based microfluidic device for single cell trapping is designed using a combination of stagnation point flow and physical barrier.The microfluidic device overcomes the weakness of the traditional ones, which have been only based upon either stagnation point flows or physical barriers, and can conveniently load dynamic biochemical signals to the trapped cell. In addition, it can connect with a programmable syringe pump and a microscope to constitute an integrated experimental system.It is experimentally verified that the microfluidic system can trap single cells in vitro even under flow disturbance and conveniently load biochemical signals to the trapped cell. The designed micro-device would provide a simple yet effective experimental platform for further study of the interactions between single cells and their microenvironments.
文摘Analyzed the relation between time delay difference and time delay estimation errors, based on the principles of three-point locating, a reformed threshold method for time delay difference estimation of impulse signals, called as amendment estimation for short, is developed by introducing channel equalization technique to its conventional version, named as direct estimation in this paper, to improve the estimation stability. After inherent relationship between time delay and phase shift of signals is analyzed, an integer period error compensation method utilized the diversities of both contribution share and contribution mode of concerned estimates is proposed under the condition of high precision phase lag estimation. Finally, a cooperative multi-threshold estimation method composed of amendment and direct estimations to process impulse signals with three thresholds is established. In sea trials data tests of passive locating, this method improves the estimation precision of time delay difference efficiently. The experiments verify the theoretical predictions.
文摘A point-of-care test system has been studied in this paper.It was used to determine substances in blood such as Hemoglobin (HB),Aspartate Aminotransferase (AST),Creatine Kinase (CK) and so on.Based on the principle of amperometric determination,the research on detecting weak current signals was carried on.At the same time as to the weak signals (nA level),magnifying,sampling and processing the signals were also studied.Controlled by ADUC824 and assisted by other units, every substance could be determined automatically and rapidly integrated with the corresponding biosensor.In the experiment, the minimum detectable current of the instrument (YT2005-1) is 0.2 nA.With regard to the 1 nA which the experiment demanded,it could be up to the mustard.And the system can provide results in 180 s with a long term stability.
基金Sponsored by the National High Technology Research and Development Program of China(Grant No.2014AA123103)
文摘A Wi-Fi fingerprinting localization approach has attracted increasing attention in recent years due to the ubiquity of Access Point( AP). However,typical fingerprinting localization methods fail to resist accidental environmental changes,such as AP movement. In order to address this problem,a robust fingerprinting indoor localization method is initiated. In the offline phase,three attributes of Received Signal Strength Indication( RSSI) —average,standard deviation and AP's response rate—are computed to prepare for the subsequent computation. In this way,the underlying location-relevant information can be captured comprehensively. Then in the online phase, a three-step voting scheme-based decision mechanism is demonstrated, detecting and eliminating the part of AP where the signals measured are severely distorted by AP 's movement. In the following localization step,in order to achieve accuracy and efficiency simultaneously,a novel fingerprinting localization algorithm is applied. Bhattacharyya distance is utilized to measure the RSSI distribution distance,thus realizing the optimization of MAximum Overlapping algorithm( MAO). Finally,experimental results are displayed,which demonstrate the effectiveness of our proposed methods in eliminating outliers and attaining relatively higher localization accuracy.
基金Supported by Visiting Researcher Foundation Granted by the National Key Laboratory of Pattern Recognition, Institute of Automation, Academic Sinicathe National Key Laboratory of Intelligence System and Technology, TsingHua University and Young Faculty
文摘In this paper, conditions for the existence of equilibrium points and global stability are emphatically discussed for Bidirectional Associative Memory(BAM) models with axonal signal transmission delays, and the discussed methods are more general. The correctness of obtained conclusions is verified by use of some examples. The obtained results have primary significance in the design and application of BAM.
基金supported by the National Natural Science Foundation of China,No.31100711,51377045,31300818the Natural Science Foundation of Hebei Province,No.H2013202176
文摘Here, we administered repeated-pulse transcranial magnetic stimulation to healthy people at the left Guangming （GB37） and a mock point, and calculated the sample entropy of electroencephalo- gram signals using nonlinear dynamics. Additionally, we compared electroencephalogram sample entropy of signals in response to visual stimulation before, during, and after repeated-pulse tran- scranial magnetic stimulation at the Guangming. Results showed that electroencephalogram sample entropy at left （F3） and right （FP2） frontal electrodes were significantly different depending on where the magnetic stimulation was administered. Additionally, compared with the mock point, electroencephalogram sample entropy was higher after stimulating the Guangming point. When visual stimulation at Guangming was given before repeated-pulse transcranial magnetic stimula- tion, significant differences in sample entropy were found at five electrodes （C3, Cz, C4, P3, T8） in parietal cortex, the central gyrus, and the right temporal region compared with when it was given after repeated-pulse transcranial magnetic stimulation, indicating that repeated-pulse transcranial magnetic stimulation at Guangming can affect visual function. Analysis of electroencephalogram revealed that when visual stimulation preceded repeated pulse transcranial magnetic stimulation, sample entropy values were higher at the C3, C4, and P3 electrodes and lower at the Cz and T8 electrodes than visual stimulation followed preceded repeated pulse transcranial magnetic stimula- tion. The findings indicate that repeated-pulse transcranial magnetic stimulation at the Guangming evokes different patterns of electroencephalogram signals than repeated-pulse transcranial mag- netic stimulation at other nearby points on the body surface, and that repeated-pulse transcranial magnetic stimulation at the Guangrning is associated with changes in the complexity of visually evoked electroencephalogram signals in parietal regions, central gyrus, and temporal regions.
文摘为了提高量子数字信号表示的灵活性,本文提出了一种新的基于补码浮点表示的一维有限长度量子数字信号表示模型(Complement Floating-point Representation of Digital Signals,CFRDS)。该模型使用两组量子比特序列分别表示位置信息与幅值信息。其中,位置信息采用有符号定点整数补码形式表示,保证了信号位置的精确性和负数处理能力;幅值信息则采用浮点数形式表示,浮点数的阶码和尾数均采用补码形式,能够更灵活地应对不同幅值的信号,确保在极端数值条件下依然保持高精度,同时,这种表示方法也简化了数学运算,能够处理更广泛的信号类型。该模型不仅在信号幅值的表示范围与精度上取得了显著提升,还在数学运算的便捷性方面展现了优越性,使得各种信号处理算法更加高效和可靠,适用于更加复杂的信号处理算法,提高了信号处理的效率。本文提出了CFRDS模型,设计了该模型的量子制备线路与基于该模型的量子数字信号基本运算线路,包括两个量子数字信号的序列加法、序列乘法以及自相关函数序列运算,并深入分析了线路复杂度,最后通过计算机仿真实验验证了所提出方案的可行性和有效性。
文摘点云的处理、传输、语义分割等是3维计算机视觉领域重要的分析任务.现如今,图神经网络和图结构在点云研究方面的有效性已被证实,基于图的点云(graph-based point cloud,GPC)研究不断涌现.因此,一种统一的研究角度、框架和方法论亟待形成.系统性梳理了GPC研究的各种应用场景,包括配准、降噪、压缩、表示学习、分类、分割、检测等任务,概括出GPC研究的一般性框架,提出了一条覆盖当前GPC全域研究的技术路线.具体来说,给出了GPC研究的分层概念范畴,包括底层数据处理、中层表示学习、高层识别任务;综述了各领域中的GPC模型或算法,包括静态和动态点云的处理算法、有监督和无监督的表示学习模型、传统或机器学习的GPC识别算法;总结了其中代表性的成果及其核心思想,譬如动态更新每层特征空间对应的最近邻图、分层以及参数共享的动态点聚合模块,结合图划分和图卷积提高分割精度;对比了模型性能,包括总体精度(overall accuracy,OA)、平均精度(mean accuracy,mAcc)、平均交并比(mean intersection over union,mIoU);在分析比较现有模型和方法的基础上,归纳了GPC目前面临的主要挑战,提出相应的研究问题,并展望未来的研究方向.建立的GPC研究框架具有一般性和通用性,为后续研究者从事GPC这个新型交叉领域研究提供了领域定位、技术总结及宏观视角.点云研究的出现,是探测器硬件技术长足进步后应运而生的结果;点云研究的现状表明在理论和实践之间存在一些挑战,一些关键问题还有待解决.同时,点云研究的发展将推动人工智能进入新的时代.
