Abnormally activated wingless/integrated signaling modulates tumor-associated macrophage polarization and potentially promotes hepatocarcinoma cell growth

In this article,we comment on the article by Huang et al.The urgent development of new therapeutic strategies targeting macrophage polarization is critical in the fight against liver cancer.Tumor-associated macrophages(TAMs),primarily of the M2 subtype,are instrumental in cellular communication within the tumor microenvironment and are influenced by various signaling pathways,including the wingless/integrated(Wnt)pathway.Activation of the Wnt signaling pathway is pivotal in promoting M2 TAMs polarization,which in turn can exacerbate hepatocarcinoma cell proliferation and migration.This manuscript emphasizes the burgeoning significance of the Wnt signaling pathway and M2 TAMs polarization in the pathogenesis and progression of liver cancer,highlighting the potential therapeutic benefits of inhibiting the Wnt pathway.Lastly,we point out areas in Huang et al’s study that require further research,providing guidance and new directions for similar studies.

Design of high-speed signal acquisition system and analysis of signal integrity

The device is used for the test on the fuze detonating time according to the initial velocity of the projectile and the altitude and speed of enemy aircraft flight. For the special requirements of the high-speed signal acquisition in the process, the characteristics of the measured signal are analyzed. The system is investigated in chip selection, signal transmission, signal processing, signal storage, post-production PCB design, etc. The appropriate measures and solutions which affect the integrity and accuracy of the signal in each process are proposed. The rules for the layout of the device and wiring are made. The result show that the measurement values are accurate without loss of data.

Gga-miRNA-181-5p family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-βsignaling

MicroRNAs(miRNAs)have been demonstrated to control chicken skeletal muscle growth,however,the potential function of the miR-181-5p family in chicken myogenesis remains largely unknown.Here,our study identified the two chicken(Gallus gallus;Gga)miR-181-5p family members widely expressed in various tissues,specifically miR-181a-5p and miR-181b-5p.Besides,the breast muscles of fast-growing broilers expressed higher levels of miR-181a-5p and miR-181b-5p than those of slow-growing layers.Functionally,miR-181a-5p and miR-181b-5p both promote the expression level of myogenic factors including myogenin(MyoG),myogenic differentiation 1(MyoD1),and myosin heavy chain(MyHC),meanwhile accelerating the myotube formation of skeletal muscle satellite cells(SMSCs).Mechanistically,miR-181a-5p and miR-181b-5p directly bind to the 3′untranslated region(UTR)of the transforming growth factor beta receptor 1(TGFBR1)mRNA,further reducing the expression of TGFBR1.TGFBR1 is a key Transforming growth factor beta(TGF-β)signaling transduction receptor and had a negative function in muscle cell differentiation.Furthermore,knockdown of TGFBR1 facilitated the expression of chicken myogenic factors,boosted myotube formation,and decreased the SMAD family member 2/3(SMAD2/3)phosphorylation in chicken SMSCs.SMAD2/3 are downstream of TGF-βsignaling,and miR-181a-5p and miR-181b-5p could reduce the expression of TGFBR1 to further diminish the SMAD2/3 phosphorylation.Our findings revealed that the miR-181-5p family targets TGFBR1 to break the TGF-βsignaling transduction,which resulted in promoting chicken skeletal muscle development.

Obstacles to the Integrated Care of Children Aged 6 to 59 Months Suffering from Acute and Severe Malnutrition at the Chu-Me and HATC of N’Djamena/Chad

Introduction: the problem of Severe Acute Malnutrition (SAM) and its consequences concern and challenge all social actors worldwide. The objective of the study was to identify the real obstacles that actors face in the integrated management of children aged 6 - 59 months suffering from SAM at the University Hospital of Mother and Child (CHUME) and the Chad-China Friendship Hospital (HATC) of N’Djamena. Methodology: this is a cross-sectional descriptive and interpretative study conducted from January to October 2023 at the CHUME and HATC of N’Djamena. It is based on questionnaires and direct observation of two hundred and thirty-six (236) parents or guardians of malnourished children admitted to these health facilities. All 236 agreed to participate freely in this study. Results: it appears from this study that the obstacles to the integrated management of malnourished children were: poor care-care relationship (26.7%) permanent product breaks (22.9%), the direct costs of services ranged from 30,000 to 60,000 FCFA (41.53%), drugs used for the treatment of patients came from the street (66.10%), malaria and anemia occupy an important place (27.5%), 57.2% respondents had monthly income below 60,000 CFAF. Conclusion: the present study carried out has identified the real bottlenecks to the integrated management of children suffering from SAM in the therapeutic nutritional units of the city of N’Djamena. .

ROS-hormone interaction in regulating integrative défense signaling of plant cell

The elaborate redox network of the cell,comprising of events like turnover of reactive oxygen species(ROS),redox sensing,signaling,expression of redox-sensitive genes,etc.,often orchestrates with other bonafide hormonal signaling pathways through their synergistic or antagonistic action in the plant cell.The redox cue generated in plant cells under fluctuating environmental conditions can significantly influence other hormonal biosynthetic or signaling mechanisms,thereby modulating physiology towards stress acclimation and defense.There is also strong evidence of the recruitment of ROS as a‘second messenger’in different hormonal signaling pathways under stress.Moreover,the retrograde signaling initiated by ROS also found to strongly influence hormonal homeostasis and signaling.The present review,in this aspect,is an effort towards understanding the regulatory roles of ROS in integrating and orchestrating other hormonal signaling pathways or vice versa so as to unfold the relationship between these two signaling episodes of plant cells under environmental odds.We also accentuate the significance of understanding the utterly complex interactions,which occur both at metabolic and genetic levels between ROS and phytohormones during stress combinations.Furthermore,the significant and decisive role of ROS turnover,particularly the contribution of RBOH(respiratory burst oxidase homologs)in the synergism of redox and hormone signaling during systemic acquired acclimation under stress is also discussed.

Quick System-Level DDR3 Signal Integrity Simulation Research

---Double data rate synchronous dynamic random access memory （DDR3） has become one of the most mainstream applications in current server and computer systems. In order to quickly set up a system-level signal integrity （SI） simulation flow for the DDR3 interface, two system-level SI simulation methodologies, which are board-level S-parameter extraction in the frequency-domain and system-level simulation assumptions in the time domain, are introduced in this paper. By comparing the flow of Speed2000 and PowerSI/Hspice, PowerSI is chosen for the printed circuit board （PCB） board-level S-parameter extraction, while Tektronix oscilloscope （TDS7404） is used for the DDR3 waveform measurement. The lab measurement shows good agreement between simulation and measurement. The study shows that the combination of PowerSI and Hspice is recommended for quick system-level DDR3 SI simulation.

Signal and Power Integrity Challenges for High Density System-on-Package

As the increasing desire for more compact,portable devices outpaces Moore’s law,innovation in packaging and system design has played a significant role in the continued miniaturization of electronic systems.Integrating more active and passive components into the package itself,as the case for system-on-package(SoP),has shown very promising results in overall size reduction and increased performance of electronic systems.With this ability to shrink electrical systems comes the many challenges of sustaining,let alone improving,reliability and performance.The fundamental signal,power,and thermal integrity issues are discussed in detail,along with published techniques from around the industry to mitigate these issues in SoP applications.

Signal Integrity for 0.18μm CMOS Technology

The signal integrity problem in 0.18μm CMOS technology is analyzed from simulation.Several rules in this phenomenon are found by analyzing the crosstalk delay and noise,which are helpful for the future circuit design.

粉碎机齿轮箱EEMD-DWT故障信号降噪及诊断分析

为了提高粉碎机齿轮箱故障诊断能力,综合运用集成经验模态(EEMD)算法和离散小波变换(DWT)的降噪技术,设计了一种通过EEMD-DWT进行降噪的新技术,确保在去除噪声的前提下可以保留有用特征。以EEMD-DWT方法降噪处理得到了光滑的信号,对信号波形的特征也实现理想复原,实现了优异降噪性能。研究结果表明:经过EEMD-DWT降噪后形成了具有明显冲击特征的波形,对幅值在零附近的噪声分量起到显著抑制效果,实现在去除噪声的条件下保留原有的振动特征。本设计的EEMD-DWT降噪方法与其它单独降噪方法相比具备更优降噪性能,能够满足粉碎机齿轮箱振动过程的实际降噪分析要求。该研究能够有效弥补EEMD在振动信号降噪方面的缺陷,提高齿轮箱的故障识别效率,也可应用到其它传动机构上,具有很高的推广价值。

研究生专业技术课程科教融合的创新实践探索--以微弱信号检测与处理课程为例

研究生教育是我国高等教育体系的重要组成部分,肩负着培养具备创新能力的科技领域人才的神圣使命。在新工科背景下,如何实现课堂教学与科学研究融合、达到学术素养和工程能力共同提升的培养目标,已成为高校教育教学改革面临的新课题。该文选取北京航空航天大学仪器科学与技术和光学工程两个一级学科开设的专业技术课微弱信号检测与处理,阐述在研究生专业技术课程教学中,通过融入本学科在国内具有影响力和技术优势的光纤陀螺、光纤电流互感器、空芯光纤微弱散射光检测三个典型科研实例,进行科教融合、提高教学质量的创新实践探索。

Inhibition of Notch 1 signaling in the subacute stage after stroke promotes striatal astrocyte-derived neurogenesis

Inhibition of Notch1 signaling has been shown to promote astrocyte-derived neurogenesis after stroke.To investigate the regulatory role of Notch1 signaling in this process,in this study,we used a rat model of stroke based on middle cerebral artery occlusion and assessed the behavior of reactive astrocytes post-stroke.We used theγ-secretase inhibitor N-[N-(3,5-diuorophenacetyl)-1-alanyl]-S-phenylglycine t-butylester(DAPT)to block Notch1 signaling at 1,4,and 7 days after injury.Our results showed that only administration of DAPT at 4 days after stroke promoted astrocyte-derived neurogenesis,as manifested by recovery of white matter fiber bundle integrity on magnetic resonance imaging,which is consistent with recovery of neurologic function.These findings suggest that inhibition of Notch1 signaling at the subacute stage post-stroke mediates neural repair by promoting astrocyte-derived neurogenesis.

基于功率控制环扰动的DC-DC变换器能量信息一体化研究

针对实现DC-DC变换器之间的信息交互较为复杂的问题,提出了一种基于功率控制环扰动实现其能量信息一体化的方法。通过在DC-DC变换器的传统功率控制环中叠加二进制频移键控(binary frequency shift keying,2FSK)信号作为扰动,将数据信息叠加到传统脉冲宽度调制(pulse-width modulation,PWM)信号中,使得该PWM信号同时包含数据信息,实现能量信息一体化。首先,给出基于2FSK的功率/数据双载波调制以及实时的滑动离散傅里叶变换(sliding discrete Fourier transform,SDFT)解调方案。然后,通过对Buck变换器的能量信息一体化模型进行小信号建模与分析可知,降低输入阻抗可提升通信的信噪比。最后搭建一个由两个Buck变换器并联的实验装置进行验证。实验结果表明在稳态和负载突变的工况下均可实现3 kb/s的稳定通信,验证了所提方法的可行性。该方法使得DC-DC变换器能同时进行功率变换和数据传输,提升数字化和智能化水平。

Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility

Background Comparative and comprehensive omics studies have recently been conducted to provide a comprehensive understanding of the biological mechanisms underlying infertility.However,because these huge omics datasets often contain irrelevant information,editing strategies for summarizing and filtering the data are necessary prerequisite steps for identifying biomarkers of male fertility.Here,we attempted to integrate omics data from spermatozoa with normal and below-normal fertility from boars and bulls,including transcriptomic,proteomic,and metabolomic data.Pathway enrichment analysis was conducted and visualized using g:Profiler,Cytoscape,EnrichmentMap,and AutoAnnotation to determine fertility-related biological functions according to species.Results In particular,gamete production and protein biogenesis-associated pathways were enriched in bull spermatozoa with below-normal fertility,whereas mitochondrial-associated metabolic pathways were enriched in boar spermatozoa with normal fertility.These results indicate that below-normal fertility may be determined by aberrant regulation of protein synthesis during spermatogenesis,and the modulation of reactive oxygen species generation to maintain capacitation and the acrosome reaction governs boar sperm fertility.Conclusion Overall,this approach demonstrated that distinct molecular pathways drive sperm fertility in mammals in a species-dependent manner.Moreover,we anticipate that searching for species-specific signaling pathways may aid in the discovery of fertility-related biomarkers within large omics datasets.

Novel non-coherent integration method using binary phase-coded radar signal

The m series with 511 bits is taken as an example being applied in non-coherent integra- tion algorithm. A method to choose the bi-phase code is presented, which is 15 kinds of codes are picked out of 511 kinds of m series to do non-coherent integration. It is indicated that the power in- creasing times of larger target sidelobe is less than the power increasing times of smaller target main- lobe because of the larger target＇ s pseudo-randomness. Smaller target is integrated from larger tar- get sidelobe, which strengthens the detection capability of radar for smaller targets. According to the sidelobes distributing characteristic, a method is presented in this paper to remove the estimated sidelobes mean value for signal detection after non-coherent integration. Simulation results present that the SNR of small target can be improved approximately 6. 5 dB by the proposed method.

Integrated system of traditional THz time-domain spectroscopy and asynchronous optical sampling

Terahertz time-domain spectroscopy(THz-TDS)system,as a new means of spectral analysis and detection,plays an increasingly pivotal role in basic scientific research.However,owing to the long scanning time of the traditional THz-TDS system and the complex control of the asynchronous optical scanning(ASOPS)system,which requires frequent calibration,we combine traditional THz-TDS and ASOPS systems to form a composite system and propose an all-fiber trigger signal generation method based on the time overlapping interference signal generated by the collinear motion of two laser pulses.Finally,the time-domain and frequency-domain spectra are obtained by using two independent systems in the integrated systems.It is found that the full width at half maximum(FWHM)of the time-domain spectra and the spectral width of the frequency-domain spectra are almost the same,but the sampling speed of the ASOPS system is significantly faster than that of the traditional THz-TDS system,which conduces to the study of the transient characteristics of substances.

Reduction of signal reflection along through silicon via channel in high-speed three-dimensional integration circuit

The through silicon via （TSV） technology has proven to be the critical enabler to realize a three-dimensional （3D） gigscale system with higher performance but shorter interconnect length. However, the received digital signal after trans- mission through a TSV channel, composed of redistribution layers （RDLs）, TSVs, and bumps, is degraded at a high data-rate due to the non-idealities of the channel. We propose the Chebyshev multisection transformers to reduce the signal reflec- tion of TSV channel when operating frequency goes up to 20 GHz, by which signal reflection coefficient （$11） and signal transmission coefficient （$21） are improved remarkably by 150% and 73.3%, respectively. Both the time delay and power dissipation are also reduced by 4% and 13.3%, respectively. The resistance-inductance-conductance-capacitance （RLGC） elements of the TSV channel are iterated from scattering （S）-parameters, and the proposed method of weakening the signal reflection is verified using high frequency simulator structure （HFSS） simulation software by Ansoft.

Study on key technologies of GNSS-based train state perception for traincentric railway signaling

The application of Global Navigation Satellite Systems(GNSSs)in the intelligent railway systems is rapidly developing all over the world.With the GNSs-based train positioning and moving state perception,the autonomy and flexibility of a novel train control system can be greatly enhanced over the existing solutions relying on the track-side facilities.Considering the safety critical features of the railway signaling applications,the GNSS stand-alone mode may not be sufficient to satisfy the practical requirements.In this paper,the key technologies for applying GNSS in novel train-centric railway signaling systems are investigated,including the multi-sensor data fusion,Virtual Balise(VB)capturing and messaging,train integrity monitoring and system performance evaluation.According to the practical characteristics of the novel train control system under the moving block mode,the details of the key technologies are introduced.Field demonstration results of a novel train control system using the presented technologies under the practical railway operation conditions are presented to illustrate the achievable performance feature of autonomous train state perception using BeiDou Navigation Satellite System(BDS)and related solutions.It reveals the great potentials of these key technologies in the next generation train control system and other GNSS-based railway implementations.

18β-glycyrrhetinic acid promotes gastric cancer cell autophagy and inhibits proliferation by regulating miR-328-3p/signal transducer and activator of transcription 3

BACKGROUND Gastric cancer(GC)is one of the most common cancer types worldwide,and its prevention and treatment methods have garnered much attention.As the active ingredient of licorice,18β-glycyrrhetinic acid(18β-GRA)has a variety of pharmacological effects.The aim of this study was to explore the effective target of 18β-GRA in the treatment of GC,in order to provide effective ideas for the clinical prevention and treatment of GC.AIM To investigate the mechanism of 18β-GRA in inhibiting cell proliferation and promoting autophagy flux in GC cells.METHODS Whole transcriptomic analyses were used to analyze and screen differentially expressed microRNAs(miRNAs)in GC cells after 18β-GRA intervention.Lentivirus-transfected GC cells and the Cell Counting Kit-8 were used to detect cell proliferation ability,cell colony formation ability was detected by the clone formation assay,and flow cytometry was used to detect the cell cycle and apoptosis.A nude mouse transplantation tumor model of GC cells was constructed to verify the effect of miR-328-3p overexpression on the tumorigenicity of GC cells.Tumor tissue morphology was observed by hematoxylin and eosin staining,and microtubule-associated protein light chain 3(LC3)expression was detected by immunohistochemistry.TransmiR,STRING,and miRWalk databases were used to predict the relationship between miR-328-3p and signal transducer and activator of transcription 3(STAT3)-related information.Expression of STAT3 mRNA and miR-328-3p was detected by quantitative polymerase chain reaction(qPCR)and the expression levels of STAT3,phosphorylated STAT3(p-STAT3),and LC3 were detected by western blot analysis.The targeted relationship between miR-328-3p and STAT3 was detected using the dual-luciferase reporter gene system.AGS cells were infected with monomeric red fluorescent protein-green fluorescent protein-LC3 adenovirus double label.LC3 was labeled and autophagy flow was observed under a confocal laser microscope.RESULTS The expression of miR-328-3p was significantly upregulated after 18β-GRA intervention in AGS cells(P=4.51E-06).Overexpression of miR-328-3p inhibited GC cell proliferation and colony formation ability,arrested the cell cycle in the G0/G1 phase,promoted cell apoptosis,and inhibited the growth of subcutaneous tumors in BALB/c nude mice(P<0.01).No obvious necrosis was observed in the tumor tissue in the negative control group(no drug intervention or lentivirus transfection)and vector group(the blank vector for lentivirus transfection),and more cells were loose and necrotic in the miR-328-3p group.Bioinformatics tools predicted that miR-328-3p has a targeting relationship with STAT3,and STAT3 was closely related to autophagy markers such as p62.After overexpressing miR-328-3p,the expression level of STAT3 mRNA was significantly decreased(P<0.01)and p-STAT3 was downregulated(P<0.05).The dual-luciferase reporter gene assay showed that the luciferase activity of miR-328-3p and STAT33’untranslated regions of the wild-type reporter vector group was significantly decreased(P<0.001).Overexpressed miR-328-3p combined with bafilomycin A1(Baf A1)was used to detect the expression of LC3 II.Compared with the vector group,the expression level of LC3 II in the overexpressed miR-328-3p group was downregulated(P<0.05),and compared with the Baf A1 group,the expression level of LC3 II in the overexpressed miR-328-3p+Baf A1 group was upregulated(P<0.01).The expression of LC3 II was detected after intervention of 18β-GRA in GC cells,and the results were consistent with the results of miR-328-3p overexpression(P<0.05).Additional studies showed that 18β-GRA promoted autophagy flow by promoting autophagosome synthesis(P<0.001).qPCR showed that the expression of STAT3 mRNA was downregulated after drug intervention(P<0.05).Western blot analysis showed that the expression levels of STAT3 and p-STAT3 were significantly downregulated after drug intervention(P<0.05).CONCLUSION 18β-GRA promotes the synthesis of autophagosomes and inhibits GC cell proliferation by regulating the miR-328-3p/STAT3 signaling pathway.

Antitumor activity of miR-188-3p in gastric cancer is achieved by targeting CBL expression and inactivating the AKT/mTOR signaling

BACKGROUND Altered miR-188-3p expression has been observed in various human cancers.AIM To investigate the miR-188-3p expression,its roles,and underlying molecular events in gastric cancer.METHODS Fifty gastric cancer and paired normal tissues were collected to analyze miR-188-3p and CBL expression.Normal and gastric cancer cells were used to manipulate miR-188-3p and CBL expression through different assays.The relationship between miR-188-3p and CBL was predicted bioinformatically and confirmed using a luciferase gene reporter assay.A Kaplan-Meier analysis was used to associate miR-188-3p or CBL expression with patient survival.A nude mouse tumor cell xenograft assay was used to confirm the in vitro data.RESULTS MiR-188-3p was found to be lower in the plasma of gastric cancer patients,tissues,and cell lines compared to their healthy counterparts.It was associated with overall survival of gastric cancer patients(P<0.001),tumor differentiation(P<0.001),lymph node metastasis(P=0.033),tumor node metastasis stage(I/II vs III/IV,P=0.024),and American Joint Committee on Cancer stage(I/II vs III/IV,P=0.03).Transfection with miR-188-3p mimics reduced tumor cell growth and invasion while inducing apoptosis and autophagy.CBL was identified as a direct target of miR-188-3p,with its expression antagonizing the effects of miR-188-3p on gastric cancer(GC)cell proliferation by inducing tumor cell apoptosis and autophagy through the inactivation of the Akt/mTOR signaling pathway.The in vivo data confirmed antitumor activity via CBL downregulation in gastric cancer.CONCLUSION The current data provides ex vivo,in vitro,and in vivo evidence that miR-188-3p acts as a tumor suppressor gene or possesses antitumor activity in GC.

Chirp-DS-BPSK雷达通信一体化波形设计

针对线性调频-二进制相移键控(Chirp-Binary Phase Shift Keying,Chirp-BPSK)雷达通信一体化信号存在模糊函数受通信数据严重影响的问题,分析了传统周期扩频、混沌序列扩频及Logistic映射混沌序列的自相关性能,设计了一种扩频信号与线性调频脉冲相结合的雷达通信一体化共用波形,即Chirp-DS-BPSK一体化信号。该一体化波形分别采用混沌序列和BPSK方式对通信数据进行直接序列扩频与调制,以线性调频信号作为载波,并采用脉冲成型滤波器来提高频谱利用率,降低脉压旁瓣。最后,通过仿真分析证明了所提波形具有良好的模糊函数特性和误码率性能。在扩频比为7的条件下,与传统的Chirp-BPSK波形相比,Chirp-DS-BPSK波形的脉压峰值旁瓣比降低了约2.69 dB,通信误码率下降约8.45 dB,表明Chirp-DS-BPSK比Chirp-BPSK更适合作为雷达通信一体化共用波形。