A small signal coupling model for predicting the coupling between LNAs

A small signal coupling model is developed to analyze the coupling between two LNAs. The mutual inductance between the adjacent on-chip inductors is considered responsible for this coupling. A set of formulas have been derived to quantitatively predict the coupling effects. Based on our analysis, a quick estimation can be made to see which pair of inductors plays a key role in evaluating the coupling between the LNAs. Source inductors of two LNAs are placed closely while the load inductors are far apart according to the analysis. To validate the proposed theory, two 2 GHz LNAs are fabricated. The LNAs have a peak gain of 18 dB and NF of 1.4 dB. The coupling between the LNAs is –30 dB.

Symbolic transfer entropy-based premature signal analysis

In this paper, we use symbolic transfer entropy to study the coupling strength between premature signals. Numerical experiments show that three types of signal couplings are in the same direction. Among them, normal signal coupling is the strongest, followed by that of premature ventricular contractions, and that of atrial premature beats is the weakest. The T test shows that the entropies of the three signals are distinct. Symbolic transfer entropy requires less data, can distinguish the three types of signals and has very good computational efficiency.

Broadband optical frequency comb generation based on single electro‑absorption modulation driven by radio frequency coupled signals

Broadband optical frequency comb(OFC)generation based on a single electro-absorption modulator(EAM)is proposed.The EAM is driven by a radio frequency(RF)multi-frequency signal generated by a multiplication coupler composed of an electrical power splitter and an arithmetic circuit.Thus the number of comb-lines of the generated OFC can be increased.A complete theoretical model of OFC generation by an EAM driven by nth power of the RF source is established,and the performance of the OFC is analyzed by using OptiSystem software.The results show that,the number of comb-lines of the OFC is positively correlated with the number of multiplication of the RF source signal.The frequency spacing of the comb-lines is twice the frequency of the RF source signal and is tunable by adjusting the frequency of the RF source signal.Increasing chirp factor and modulation index of EAM could increase the number of comb-lines of the generated OFC.The amplitude of the RF source signal had little impact on the fatness of the OFC and the average OFC power.The scheme developed is not only simple and low-cost,but also can produce a large number of comb-lines.

Jigsaw-like mini-pillar platform for multi-mode biosensing

The multiple sensing provides booming options to eliminate interference and ensure the accuracy of detection by mutually coupling and validating multiple data sets.Here,we integrate the jigsaw-like multifunctional mini-pillar platform to perform multi-mode(electrochemical,fluorescence,surface-enhanced Raman scattering(SERS)and colorimetric)sensing in individual microdroplets.Each mini-pillar connector can parallelize together by specific concave-convex interface to form integrated jigsaw-like platform for multi-mode sensing,and each specific mini-pillar can be modified into the individual sensing unit to read the prescribed signals.We successfully implemented electrochemical,fluorescence,SERS and colorimetric detection by multiple signals coupling to reduce the false positive analysis.Such platform brings a promising clue of in-situ analysis and point-of-care testing for disease diagnosis and health monitoring.

G protein b_1λ_2 subunits purification and their interaction with adenylyl cyclase

A preliminary study on the interaction of G protein (guanine triphosphate binding pro- tein) b1g2 subunits and their coupled components in cell signal transduction was conducted in vitro. The insect cell lines, Sf9 (Spodoptera frugiperda) and H5 (Trichoplusia ni ) were used to express the recombinant protein Gb1g2. The cell membrane containing Gb1g2 was isolated through affinity chromatography column with Ni-NTA agarose by FPLC method, and the highly purified protein was obtained. The adenylyl cyclase 2 (AC2) activity assay showed that the purified Gb1g2 could signifi-cantly stimulate AC2 activity. The interaction of b1g2 subunits of G protein with the cytoplasmic tail of various mammalian adenylyl cyclases was monitored by BIAcore technology using NTA sensor chip, which relies on the phenomenon of surface plasmon resonance (SPR). The experiments showed the direct binding of Gb1g2 to the cytoplasmic tail C2 domain of AC2. The specific binding domain of AC2 with Gb1g2 was the same as AC2 activity domain which was stimulated by Gb1g2.