A fully automatic setup for on-wafer contact probing will be presented. This setup consists of six automatable nano positioning axes used as tool holder and a sample holder. With this setup a fully automatic one-port ...A fully automatic setup for on-wafer contact probing will be presented. This setup consists of six automatable nano positioning axes used as tool holder and a sample holder. With this setup a fully automatic one-port SOL calibration for a Vector Network Analyzer is done. Furthermore a fully automated on-wafer contact probing is performed. Afterwards, the effects of a misalignment of the three tips of a GSG-probe are examined. Additionally the error on the calibration is calculated to determine its effect on the measurement. The results show, that a misalignment of the probe has a high impact on the measurement of the VNA. Hence a fully automated on-wafer probing presented in this paper is a good way to detect these misalignments and correct them if necessary.展开更多
A promoter\|signal peptide probe vector of lactic acid bacteria,designated pZLB based on pMG36e has been constructed.The vector contained a reporterd gene encoding for β lactamase,which lacked a promoter and a functi...A promoter\|signal peptide probe vector of lactic acid bacteria,designated pZLB based on pMG36e has been constructed.The vector contained a reporterd gene encoding for β lactamase,which lacked a promoter and a functional signal sequence.Sau3A restriction fragments (80-400bp) from L.lactis ATCC 7962 total DNA were cloned into Bam HI site of pZLB.A lot of different size framents were selected.These fragments promoted transcription and secretion of β lactamase in L.lac tis. Sequence analysis revealed that inserted fragment in pZLBe7 contained typical promoter,SD sequence and atypical signal sequence.展开更多
文摘A fully automatic setup for on-wafer contact probing will be presented. This setup consists of six automatable nano positioning axes used as tool holder and a sample holder. With this setup a fully automatic one-port SOL calibration for a Vector Network Analyzer is done. Furthermore a fully automated on-wafer contact probing is performed. Afterwards, the effects of a misalignment of the three tips of a GSG-probe are examined. Additionally the error on the calibration is calculated to determine its effect on the measurement. The results show, that a misalignment of the probe has a high impact on the measurement of the VNA. Hence a fully automated on-wafer probing presented in this paper is a good way to detect these misalignments and correct them if necessary.
文摘A promoter\|signal peptide probe vector of lactic acid bacteria,designated pZLB based on pMG36e has been constructed.The vector contained a reporterd gene encoding for β lactamase,which lacked a promoter and a functional signal sequence.Sau3A restriction fragments (80-400bp) from L.lactis ATCC 7962 total DNA were cloned into Bam HI site of pZLB.A lot of different size framents were selected.These fragments promoted transcription and secretion of β lactamase in L.lac tis. Sequence analysis revealed that inserted fragment in pZLBe7 contained typical promoter,SD sequence and atypical signal sequence.
