A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes s...A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.展开更多
In order to reveal the biochemical basis for the pharmacological use of the antler velvet of Père David’s deer (Eluphurus davidianus), we analyzed the estradiol concentrations of velvet tissue samples. We collec...In order to reveal the biochemical basis for the pharmacological use of the antler velvet of Père David’s deer (Eluphurus davidianus), we analyzed the estradiol concentrations of velvet tissue samples. We collected velvet tissue from Père David’s deer, sika deer (Cervus nippon) and fallow deer (Dama dama). After extracting steroids from velvet tissue with ether, we analyzed the estrdiol concentrations using radioimmunoassay technique. We found that: (1) estradiol concentrations were significantly different in the velvet tissue of these three cervids (P<0.05) and (2) Père David’s deer stags had the highest velvet tissue estradiol concentrations, 105.29±27.53 pg/g. [Acta Zoologica Sinica 49(1):124-127,2003].展开更多
文摘A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.
文摘In order to reveal the biochemical basis for the pharmacological use of the antler velvet of Père David’s deer (Eluphurus davidianus), we analyzed the estradiol concentrations of velvet tissue samples. We collected velvet tissue from Père David’s deer, sika deer (Cervus nippon) and fallow deer (Dama dama). After extracting steroids from velvet tissue with ether, we analyzed the estrdiol concentrations using radioimmunoassay technique. We found that: (1) estradiol concentrations were significantly different in the velvet tissue of these three cervids (P<0.05) and (2) Père David’s deer stags had the highest velvet tissue estradiol concentrations, 105.29±27.53 pg/g. [Acta Zoologica Sinica 49(1):124-127,2003].
