A novel integrated nanobiocatalyst system based on an enzyme@silica nanoflower@metal-organic framework （enzyme@SNF@ZIF-8） structure with improved stability is fabricated for the first time. The versatility of this s...A novel integrated nanobiocatalyst system based on an enzyme@silica nanoflower@metal-organic framework （enzyme@SNF@ZIF-8） structure with improved stability is fabricated for the first time. The versatility of this system is validated using penicillin G acylase （PGA） and catalase （CAT） as model enzymes. The microporous ZIF-8 layer can be controlled by varying the number of ZIF-8 coating cycles, which produces PGA@SNF@ZIF-8 nanobiocatalysts with different ZIF-8 layer thicknesses. After the second ZIF-8 coating cycle, a PGA@SNF@ZIF-8（2） structure with a homogeneous and well-intergrown ZIF-8 layer is formed, which possesses excellent mechanical and chemical stability. Moreover, PGA@SNF@ZIF-8（2） shows improved thermal/storage stability and reusability compared with free PGA and PGA immobilized on silica nanoflowers （PGA@SNF）. The obtained CAT-based nanobiocatalysts （CAT@SNF@ZIF-8（2）） also show excellent catalytic performance.展开更多
Here,we reported a cancer nanovaccine based on SiO_(2)nanoflowers with a special radial pore structure,which greatly enhanced cross-presentation and induced the production of cytotoxic T lymphocyte cells secreting gra...Here,we reported a cancer nanovaccine based on SiO_(2)nanoflowers with a special radial pore structure,which greatly enhanced cross-presentation and induced the production of cytotoxic T lymphocyte cells secreting granzymes B and interferon-γ.The antigen ovalbumin was covalently conjugated onto the as-synthesized hierarchical SiO_(2)nanoflowers,and the adjuvant cytosine-phosphate-guanine was electrostatically adsorbed into their radial pore by simple mixing before use.The nanovaccine exhibited excellent storage stability without antigen release after 27 days of incubation,negligible cytotoxicity to dendritic cells,and a high antigen loading capacity of 430±66 mg·g^(−1)support.Besides,the nanovaccine could be internalized by dendritic cells via multiple pathways.And the enhancement of antigen/adjuvant uptake and lysosome escape of antigen were observed.Noteworthy,in vitro culture of bone marrow-derived dendritic cells in the presence of nanovaccine proved the activation of dendritic cells and antigen cross-presentation as well as secretion of proinflammatory cytokines.Besides,in vivo study verified the targeting of nanovaccine to draining lymph nodes,the complete suppression of tumor in six out of ten mice,and the triggering of notable tumor growth delay.Overall,the present results indicated that the nanovaccine can be served as a potential therapeutic vaccine to treat cancer.展开更多
文摘A novel integrated nanobiocatalyst system based on an enzyme@silica nanoflower@metal-organic framework （enzyme@SNF@ZIF-8） structure with improved stability is fabricated for the first time. The versatility of this system is validated using penicillin G acylase （PGA） and catalase （CAT） as model enzymes. The microporous ZIF-8 layer can be controlled by varying the number of ZIF-8 coating cycles, which produces PGA@SNF@ZIF-8 nanobiocatalysts with different ZIF-8 layer thicknesses. After the second ZIF-8 coating cycle, a PGA@SNF@ZIF-8（2） structure with a homogeneous and well-intergrown ZIF-8 layer is formed, which possesses excellent mechanical and chemical stability. Moreover, PGA@SNF@ZIF-8（2） shows improved thermal/storage stability and reusability compared with free PGA and PGA immobilized on silica nanoflowers （PGA@SNF）. The obtained CAT-based nanobiocatalysts （CAT@SNF@ZIF-8（2）） also show excellent catalytic performance.
基金the National Natural Science Foundation of China(Grant No.81972899)Natural Science Foundation of Tianjin City(Grant No.18JCQNJC14500)+1 种基金CAMS Innovation Fund for Medical Sciences(Grant No.2017-I2M-3-022)Specific Program for High-Tech Leader&Team of Tianjin Government,Tianjin innovation and promotion plan key innovation team of immunoreactive biomaterials.
文摘Here,we reported a cancer nanovaccine based on SiO_(2)nanoflowers with a special radial pore structure,which greatly enhanced cross-presentation and induced the production of cytotoxic T lymphocyte cells secreting granzymes B and interferon-γ.The antigen ovalbumin was covalently conjugated onto the as-synthesized hierarchical SiO_(2)nanoflowers,and the adjuvant cytosine-phosphate-guanine was electrostatically adsorbed into their radial pore by simple mixing before use.The nanovaccine exhibited excellent storage stability without antigen release after 27 days of incubation,negligible cytotoxicity to dendritic cells,and a high antigen loading capacity of 430±66 mg·g^(−1)support.Besides,the nanovaccine could be internalized by dendritic cells via multiple pathways.And the enhancement of antigen/adjuvant uptake and lysosome escape of antigen were observed.Noteworthy,in vitro culture of bone marrow-derived dendritic cells in the presence of nanovaccine proved the activation of dendritic cells and antigen cross-presentation as well as secretion of proinflammatory cytokines.Besides,in vivo study verified the targeting of nanovaccine to draining lymph nodes,the complete suppression of tumor in six out of ten mice,and the triggering of notable tumor growth delay.Overall,the present results indicated that the nanovaccine can be served as a potential therapeutic vaccine to treat cancer.
