Effects of Temperature on Determination of Protein Concentration with Coomassie Brilliant Blue Method

[Objective] This study aimed to investigate the effects of temperature on determination of protein concentration with Coomassie Brilliant Blue method,thus proving advice and guidance for accurate determination of protein concentration.[Method] With Coomassie Brilliant Blue method,the concentrations of different bovine serum albumin samples were determined under different temperatures and incubation time.[Result] According to the standard curve,when the determination range of protein concentration was 0-100 mg/ml,the determined protein concentration was relatively stable after incubation at 20 ℃ for 20-30 min.Furthermore,the determination result of higher protein concentration with Coomassie Brilliant Blue method was less affected by various factors.[Conclusion] In determination of protein concentration with Coomassie Brilliant Blue method,temperature,sample concentration and incubation time were important factors affecting the accuracy of experimental results.

Optical simulation of in-plane-switching blue phase liquid crystal display using the finite-difference time-domain method

The finite-difference time-domain method is used to simulate the optical characteristics of an in-plane switching blue phase liquid crystal display.Compared with the matrix optic methods and the refractive method,the finite-difference timedomain method,which is used to directly solve Maxwell＇s equations,can consider the lateral variation of the refractive index and obtain an accurate convergence effect.The simulation results show that e-rays and o-rays bend in different directions when the in-plane switching blue phase liquid crystal display is driven by the operating voltage.The finitedifference time-domain method should be used when the distribution of the liquid crystal in the liquid crystal display has a large lateral change.

Chemical Analysis Method for Magnesia and Magnesia-Alumina Refractories——Gravimetric-Molybdenum Blue Photometric Method for Determination of Silicon Dioxide Content

This standard specifies the gravimetric-molybdenum blue photometric method for determination of silicon dioxide content.

A Method to Obtain Auger Recombination Coefficient in an InGaN-Based Blue Light-Emitting Diode

We propose and demonstrate to derive the Auger recombination coefficient by fitting efficiency-current and carrier lifetime-current curves simultaneously, which can minimize the uncertainty of fitting results. The obtained Auger recombination coefficient is 1.0x10(-31) cm(6)s(-1) in the present sample, which contributes slightly to efficiency droop effect.

一种加强的主动队列管理算法——EBLUE

作为一种典型的主动队列管理算法,BLUE明显不同于其它方法,它使用丢包和连接空闲事件来控制拥塞。试验表明BLUE的丢包率明显小于RED,但是其参数设置仍然存在一些不足之处。本文在BLUE算法的基础之上,通过引进自适应的思想对其进行了改进,提出了一种加强的BLUE队列算法——EBLUE。大量的仿真实验表明本文的改进算法能够进一步提高BLUE的性能。

BV Blue法在细菌性阴道病中的诊断价值评估

目的探讨BV Blue法在细菌性阴道病(BV)中的诊断价值,为临床快速诊断BV提供可靠的方法。方法将560例患者按年龄分为4组(<30岁、31～40岁、41～50岁、>50岁),同时应用BV Blue法和Amsel标准法检测,比对分析结果。结果 BV Blue法BV阳性率为20.89%,与Amsel标准法阳性率(24.82%)比较差异无统计学意义(P>0.05)。BV Blue法诊断BV的特异性为97.86%、敏感度为77.79%、准确度为92.85%。BV阳性率随着年龄增长有上升趋势,以>50岁组的阳性率为最高(28%)。结论 BV Blue法操作简便、快速,具有较高的特异性和准确度,适用于快速诊断BV。

一种基于速率的BLUE改进方法

连接数量的显著变化会使得BLUE队列管理机制的参数失效并导致队列在丢包和空队列之间波动 分析和实验表明BLUE对于动态网络环境的适应性仍然存在不足之处 为了解决这一问题 ,通过引进自适应思想提出了一种改进方法 其主要思想就是依据数据包的到达速率自适应地调整其标记概率 大量仿真实验表明所提出的方法能够明显增强BLUE在动态网络环境下的鲁棒性 和BLUE相比 。

大鼠中枢神经向松果腺的投射──FastBlue逆行追踪研究

用荧光素Ｆａｓｔｂｌｕｅ逆行追踪法研究了大鼠松果腺的中枢性支配。结果发现标记细胞出现于脑内丘脑下部室旁核、丘脑下部前区、缰内侧核和缰外侧核等处，说明大鼠松果腺也接受来自中枢的神经纤维、受中枢的直接支配。

High efficient oxygen reduction performance of Fe/Fe3C nanoparticles in situ encapsulated in nitrogen-doped carbon via a novel microwave-assisted carbon bath method

Fe-based carbon materials are widely considered promising to replace Pt/C as next-generation electrocatalysts towards oxygen reduction reaction (ORR). However, the preparation of Fe-based carbon materials is still carried out by conventional heating method (CHM). Herein, a novel microwave-assisted carbon bath method (MW-CBM) was proposed, which only took 35 min to synthesize Fe/Fe3C nanoparticles encapsulated in N-doped carbon layers derived from Prussian blue (PB). The catalyst contained large specific surface area and mesoporous structure, abundant Fe-Nx and C–N active sites, unique core-shell structure. Due to the synergistic effects of these features, the as-prepared Fe/Fe3C@NC-2 displayed outstanding ORR activity with onset potential of 0.98 VRHE and halfwave potential of 0.87 VRHE, which were more positive than 20 wt.% Pt/C (0.93 VRHE and 0.82 VRHE). Besides, Fe/Fe3C@NC-2 gave a better stability and methanol tolerance than Pt/C towards ORR in alkaline media, too.

Spectrophotometric Determination of Fluoride in Groundwater Using Resorcin Blue Complexes

New simple and sensitive spectrophotometric determination of fluoride in drinking groundwater has been developed using aluminium-resorcin blue complex. The method is based on the reaction of fluoride with the coloured complex to produce colourless aluminium fluoride complex and releasing of the free ligand. The relationship of the reaction of flu-oride with the complex is sixth-order polynomial function. The reaction reaches equilibrium at fluoride concentration of 0.054 mM. The equilibrium constant (Keq) was calculated as 1.12 × 1014. Beer-Lambert law is obeyed in the range 0.0 - 0.024 mM of fluoride (0.0 - 1.0 mg·L-1). The molar absorptivity at 502 nm is 6.45 × 103 L·mol-1·cm-1. Fluoride concentration higher than 1.0 mg·L-1 can be measured after proper dilution. The sensitivity, detection limit, quantitation limit, and the percentage recovery of 0.75 mg·L-1 fluoride for the method were found to be 0.357 μg·ml-1., 0.07 mg·L-1, 0.2 mg·L-1, and 101.1 respectively.

Characterization and ultraviolet-visible shielding property of samarium-cerium compounds containing Sm_(2)O_(2)S prepared by co-precipitation method

Since ultraviolet(UV)light,as well as blue light,which is part of visible light,is harmful to skin,samarium-cerium compounds containing Sm_(2)O_(2)S were synthesized by co-precipitation method.This kind of compounds blocks not only UV light,but also blue light.The minimum values of average transmittance(360-450 nm)and band gap of samarium-cerium compounds were 8.90%and 2.76 eV,respectively,which were less than 13.96%and 3.01 eV of CeO_(2).Elemental analysis(EA),X-ray diffraction(XRD),Fourier transformation infrared(FTIR),and Raman spectra determined that the samples contained Ce_(4)O_(7),Sm_(2)O_(2)S,Sm_(2)O_(3),and Sm_(2)O_(2)SO_(4).The microstructure of samples was analyzed by scanning and transmission electron microscopies(SEM and TEM).X-ray photoelectron spectrum(XPS)showed that cerium had Ce^(3+)and Ce^(4+) valence states,and oxygen was divided into lattice oxygen and oxygen vacancy,which was the direct cause of the decrease of average transmittance and band gap.

Differences in Modified Morgan Phosphorus Levels Determined by Colorimetric and Inductively Coupled Plasma Methods

Phosphorus (P) fertilization is frequently needed for profitable crop production. Modified Morgan P (MMP) is a soil test P used to estimate plant available P in soils. The critical values of MMP for P fertilization and maintenance recommendations are based on the P concentrations measured by a common colorimetric molybdenum blue method although other P quantification methods have also been used for MMP measurements. In this study, we collected 120 surface soil samples of Caribou Sandy loam under potato cultivation or its rotation crops from Maine, USA, and 72 soil samples of Cecil sandy loam with cotton/corn crops under conventional tillage and no-till management with chemical and poultry litter fertilization in Georgia, USA. The MMP levels in all 192 dry samples were greater when they were measured by an inductively coupled plasma (ICP)-based method, compared to the corresponding data produced from colorimetry. Our results show the two sets of data were positively and significantly correlated (r = 0.93, P –1 with standard deviation of 12.9, compared to the average of colorimetric MMP level of 14.9 mg P kg–1 with standard deviation of 8.8. Based on the observations in this work, both colorimetric and ICP-based methods can be used for P fertilizer recommendation, but a conversion factor should be applied for ICP data as the current recommendation systems are based on colorimetric M&R data.

Electrochemical determination of sulphide at multi-walled carbon nanotubes-dihexadecyl hydrogen phosphate composite film modified electrodes based on in situ synthesis of methylene blue

A novel electrochemical method for the determination of sulphide at a multi-walled carbon nanotube-dihexadecyl hydrogen phosphate composite film coated glassy carbon electrode （MWNTs-DHP/GCE） based on in situ synthesis of methylene blue （MB） was established. 2007 Sheng Shui Hu. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

A Comparative Study on Four Staining Methods for Antitumor Active Fraction of Periplaneta americana

[ Objective ] This study aimed to compare four staining methods for proteins of SDS-polyacrylamide gel dectrophoresis （SDS-PAGE） and explore a suit- able staining method for the antitumor active fraction of P. americana after polyacrylamide gel electrophoresis. [ Method ] BSA was used as the standard for the comparison of Coomassie brilliant blue staining method, potassium staining method, calcium staining method and silver staining method, on the basis, antitumor ac- tive fraction samples of P. americana were used for SDS-PAGE electrophoresis and staining. [ Result] The results showed that silver staining method could be ac- curately, quickly and easily used for SDS-PAGE staining of the antitumor active fraction of P. amer/cana. [ Conclusion] This study laid the foundation for explo- ring the medicinal value of P. americana.

Structural, Optical and Photocatalytic Properties of Cu2+ Doped ZnO Nanorods with Using HMTA Solvent Prepared by Hydrothermal Method

In this experiment, Cu2+ doped ZnO (Cu-ZnO) nanorods materials have been fabricated by hydrothermal method. Cu2+ ions were doped into ZnO with ratios of 2, 5 and 7 mol.% (compared to the mole’s number of Zn2+). The hexamethylenetetramine (HMTA) solvent used for the fabrication of Cu-ZnO nanorods with the mole ratio of Zn2+:HMTA = 1:4. The characteristics of the materials were analyzed by techniques, such as XRD, Raman shift, SEM and UV-vis diffuse reflectance spectra (DRS). The photocatalytic properties of the materials were investigated by the decomposition of the methylene blue (MB) dye solution under ultraviolet light. The results show that the size of Cu-ZnO nanorods was reduced when the Cu2+ doping ratio increased from 2 mol.% to 7 mol.%. The decomposition efficiency of the MB dye solution reached 92% - 97%, corresponding to the Cu2+ doping ratio changed from 2 - 7 mol.% (after 40 minutes of ultraviolet irradiation). The highest efficiency for the decomposition of the MB solution was obtained at a Cu2+ doping ratio of 2 mol.%.

亚甲蓝分光光度法测定水中阴离子表面活性剂含量的方法改进

对亚甲蓝分光光度法(GB 7494—1987)测定水中阴离子表面活性剂含量进行了方法改进。以毒性相对较小的二氯甲烷取代三氯甲烷作为萃取剂,试管取代分液漏斗,利用振荡器进行萃取。结果表明:改进方法的最佳萃取时间为1.5 min,单个样品的分析时间在10 min以内;十二烷基苯磺酸钠标准曲线的线性范围在2.0 mg·L^(-1)以内,检出限(3s/k)为0.0297 mg·L^(-1);对十二烷基苯磺酸钠标准溶液重复测定6次,测定值的相对标准偏差(n=6)均小于9.0%;对空白样品进行加标回收试验,回收率为95.0%~99.1%。

NaYSiO_(4)∶Ce^(3+)蓝色荧光粉的发光性质及其在白光发光二极管上的应用

采用高温固相法合成了NaYSiO_(4)∶xCe^(3+)(0.01≤x≤0.05)系列蓝色荧光粉。NaYSiO_(4)∶xCe^(3+)荧光粉在250~360 nm之间的宽带吸收能与紫外LED芯片很好地匹配。NaYSiO_(4)∶xCe^(3+)荧光粉中存在多个Ce^(3+)离子荧光中心,且在紫外光激发下表现出峰值波长位于414 nm附近的宽带蓝光发射。NaYSiO_(4)∶0.02Ce^(3+)荧光粉在300~350 nm紫外光激发下量子效率在25%以上。NaYSiO_(4)∶0.02Ce^(3+)荧光粉表现出优良的化学稳定性,在水中浸泡14 d后荧光强度和量子效率几乎不变。将NaYSiO_(4)∶0.02Ce^(3+)蓝色荧光粉、商用(Sr,Ba)_2SiO_(4)∶Eu^(2+)绿色荧光粉和商用(Ca,Sr)AlSiN_(3)∶Eu^(2+)红色荧光粉涂覆于310 nm紫外LED芯片上制备得到了显色指数高达95的LED器件。当驱动电流从50 mA逐渐增大到300 mA时,制备的LED器件表现出稳定的暖白光发射,其色坐标几乎不变。上述结果说明,本研究报道的NaYSiO_(4)∶0.02Ce^(3+)蓝色荧光粉在紫外LED芯片驱动的白光发光二极管照明上有着潜在应用价值。

Li-Ce共掺杂TiO_(2)纳米材料制备及光催化性能的研究

选用六水合硝酸铈(Ce(NO_(3))_(3)·6H_(2)O)为掺杂剂,以钛酸四丁酯(TBOT)为原料,采用溶胶-凝胶法制备出铈掺杂改性的TiO_(2)光催化剂,并利用XRD、SEM、Uv-vis等表征方法研究Ce/TiO_(2)催化剂的结构、形貌和其对亚甲基蓝的光催化降解性能。结果表明:在500℃退火2 h,Ti与Ce物质的量比为1∶0.01(TiO_(2):0.01Ce)时,样品光催化效率最高,对亚甲基蓝降解率可达93.8%。同时还对TiO_(2):0.01Ce进行Li共掺改良,其光降解率可达95.4%,大于相同条件下单掺杂Ce的光降解率。

带诱芯的蓝板放置位置和方式对番茄潜叶蛾的诱捕效果

番茄潜叶蛾是一种重大外来入侵性有害生物,给番茄产业带来严重威胁。为明确蓝板放置方式对其诱蛾量的影响,开展了带有性诱芯的蓝板不同放置角度和不同高度对番茄潜叶蛾的诱杀效果试验。结果表明,蓝板不同放置角度和放置高度对诱蛾量影响较大。蓝板平放处理的平均诱蛾量分别是与地面角度为45°斜放和90°竖放(蓝板垂直于地面)处理的1.438倍和8.590倍,其中平放和斜放均与竖放差异达极显著水平;蓝板放置高度为0 cm处理的平均诱蛾量分别是高度30~35 cm和高度60~65cm处理的1.491倍和2.485倍,且与后者之间差异达显著水平。因此,田间采用蓝板对番茄潜叶蛾进行诱杀时,将蓝板平放在地面上的诱蛾效果最好。

ICG荧光示踪法联合亚甲蓝染色在乳腺癌患者前哨淋巴结活检中的应用价值

目的探讨乳腺癌患者前哨淋巴结活检(SLNB)中应用吲哚菁绿(ICG)荧光示踪法联合亚甲蓝染色的临床价值。方法选择118例乳腺癌患者,以随机数字表法分为2组,对照组、观察组各59例。2组均进行SLNB,术中对照组使用亚甲蓝染色法,观察组使用ICG荧光示踪法联合亚甲蓝染色。比较2组SLN检出情况及血压下降、注射部位感染、坏死、发热等并发症发生情况。结果观察组示踪成功率为98.31%(58/59),高于对照组的86.44%(51/59),差异有统计学意义(P<0.05)。观察组共检出246枚SLN,每位患者平均检出(4.17±0.36)枚;对照组共检出162枚SLN,每位患者平均检出(2.75±0.32)枚;组间对比差异有统计学意义(P<0.05)。2组患者术后均未发生明显并发症。结论与单用亚甲蓝染色相比,联合ICG荧光示踪法在乳腺癌患者SLNB中的应用效果更佳,其示踪成功率更高,检出数量更多,临床应用价值较高。