Curative effect of hydrogen peroxide combined with silver ion disinfection on pelvic floor dysfunction

BACKGROUND Pelvic floor dysfunction(PFD)is related to muscle fiber tearing during childbirth,negatively impacting postpartum quality of life of parturient.Appropriate and effective intervention is necessary to promote PFD recovery.AIM To analyze the use of hydrogen peroxide and silver ion disinfection for vaginal electrodes in conjunction with comprehensive rehabilitation therapy for postpartum women with PFD.METHODS A total of 59 women with PFD who were admitted to the hospital from May 2019 to July 2022 were divided into two groups:Control group(n=27)received comprehensive rehabilitation therapy and observation group(n=32)received intervention with pelvic floor biostimulation feedback instrument in addition to comprehensive rehabilitation therapy.The vaginal electrodes were disinfected with hydrogen peroxide and silver ion before treatment.Intervention for both groups was started 6 weeks postpartum,and rehabilitation lasted for 3 months.Pelvic floor muscle voltage,pelvic floor muscle strength,vaginal muscle voltage,vaginal muscle tone,pelvic floor function,quality of life,and incidence of postpartum PFD were compared between the two groups.RESULTS Before comprehensive rehabilitation treatment,basic data and pelvic floor function were not significantly different between the two groups.After treatment,the observation group showed significant improvements in the maximum voltage and average voltage of pelvic floor muscles,contraction time of type I and type II fibers,pelvic floor muscle strength,vaginal muscle tone,vaginal muscle voltage,and quality of life(GQOLI-74 reports),compared with the control group.The observation group had lower scores on the pelvic floor distress inventory(PFDI-20)and a lower incidence of postpartum PFD,indicating the effectiveness of the pelvic floor biostimulation feedback instrument in promoting the recovery of maternal pelvic floor function.CONCLUSION The combination of the pelvic floor biostimulation feedback instrument and comprehensive rehabilitation nursing can improve pelvic floor muscle strength,promote the recovery of vaginal muscle tone,and improve pelvic floor function and quality of life.The use of hydrogen peroxide and silver ion disinfectant demonstrated favorable antibacterial efficacy and is worthy of clinical application.

Effect of Hydrogen Reduction of Silver Ions on the Performance and Structure of New Solid Polymer Electrolyte PEI/Pebax2533/AgBF4 Composite Membranes

In this paper, the effect of hydrogen reduction of silver ions on the performance and structure of new solid polymer electrolyte polyetherimide （PEI）/Pebax2533 （Polynylonl2/tetramethylene oxide block copolymer, PA12-PTMO）/AgBF4 composite membranes is investigated. For PEI/Pebax2533/AgBF4 composite membranesprepared with dillerent AgBF4 concentration, the permeances of propylene and ethylene increase with the increase of AgBF4 concentration due to the carrier-facilitated transport, resulting in a high selectivity. But for propyl- ene/propane mixture, the mixed-gas selectivity is lower than its ideal selectivity. The hydrogen reduction strongly influences the membrane performance, which causes the decrease of propylene permeance and the increase of pro-pane permeance. With the increase of hydrogen reduction time, the membranes show a clearly color change from white to brown, yielding a great selectivity loss. The data of X-ray diffraction and FT-IR prove that silver ions are reduced to Ago after hydrogen reduction, and aggregated on the surface of PEI/Pebax2533/AgBF4 composite mem- branes.

Selective capture of silver ions from aqueous solution by series of azole derivatives-functionalized silica nanosheets

The introduction of bifunctional groups into low-cost adsorbents for selective adsorption of Ag(I) through synergistic effect will have a profound impact on the recovery of precious metals. Organo silica nanosheets(organo-Si NSs) functionalized by series of azole derivatives(2-mercaptoimidazole(MI), 2-mercaptobenzimidazole(MBT) and 1H-1,2,4-triazole-3-thiol(MTT)), are fabricated and employed for selective removal of Ag(I). The structures of the organo-Si NSs are investigated using several characterization methods. The results of batch adsorption experiments display that the maximum adsorption amounts are 70.3, 103.2 and 139.5 mg·g^(-1) on MI-Si NSs, MBI-Si NSs and MTT-Si NSs for Ag(I) ions, and reach rapid equilibrium within 10–30 min. The adsorption processes are chemisorption and fit pseudo-second-order kinetic model and Langmuir adsorption isotherm model. Notably, MTT-Si NSs is greatly selective for Ag(I) in multicomponent system, and the distribution coefficient value of Ag(I) ions reaches 2331.26 ml·g^(-1). The reusability of organo-Si NSs is verified by four cycles of regeneration tests with 0.1 mol·L^(-1) HNO_(3) as the eluent. A combination of experimental, structural along with theoretical analysis is conducted to proclaim the structure-adsorptivity relationship:(i) The adsorption mechanisms are attributed to complexation.(ii) The amino group and sulfhydryl group of MTT-Si NSs as well as MBISi NSs may have synergistic impacts on Ag(I) capture.(iii) The differences in adsorption behavior and selectivity of the three organo-Si NSs are mainly related to the form of function groups, charge density and steric hindrance of adsorbent. This work not only sheds light on the promise of functionalized organo-Si NSs for the rapid and selective removal/enrichment of Ag(I) ions in complex water systems,but also provides new insights for designing cost-effective Si NSs-based adsorbents.

DETERMINATION OF SERUM ALBUMIN USING A SILVER ION SELECTIVE ELECTRODE

In this paper is reported a new method of determining serum albumin with potentiometry. The silver ion selective electrode was used as working electrode and S. C. E as reference electrode. By controlling the ionic strength, PH and temperature constant, a good linear relationship was obtained between the cell potential and albumin concentration. This method .possessed characters-of operating simplicity, rapidity and directness. The samples could be detected directly and the results were in accordance with the values detected by classic K method.

Research progress of DNA aptamer-based silver ions detection

Silver ions are regarded as one of the most hazardous metal contaminants,endangering both the ecological environment and human health.Traditional silver ions detection technologies are hampered by their high cost,time-consuming nature,and labor-intensive operation.DNA aptamers play an essential part in the field of biosensors due to their ease of synthesis,ease of modification,and low cost.This paper focuses on reviewing the research progress of DNA aptamer-based biosensors in silver ions detection.According to the types of signal transduction,they are classified into four forms of signal transduction:fluorescent biosensors,colorimetric biosensors,electrochemical biosensors,and surface-enhanced Raman spectroscopy biosensors.In addition,this paper gives a perspective on the application prospects outlook and development directions of DNA aptamer-based biosensors,in order to provide theoretical ideas for the future development of more sensitive DNA aptamer biosensors for rapid detection of silver ions.

Silver-ion HPLC测定1,3-二油酸-2-棕榈酸三酰甘油的含量

准确测定1,3-二油酸-2-棕榈酸三酰甘油(OPO)的含量,建立了一种以高效液相色谱结合银离子交换柱(Silver-ion HPLC)的测定方法。以ChromSpher 5 Lipid(25 cm×4.6 mm,5μm)银离子交换柱通过HPLC正相系统分离三酰甘油,配合蒸发光散射检测器(ELSD),以毒性较小的二氯甲烷和丙酮为流动相。流速0.6 mL/min,柱温30℃。漂移管温度30℃,空气压力0.35 MPa。在此条件下,1,3-二油酸-2-棕榈酸三酰甘油和1,2-二油酸-3-棕榈酸三酰甘油两种同分异构体基本达到分离,样品出峰完整,峰形良好。OPO色谱峰面积的自然对数与浓度的自然对数呈良好的线性关系(R2=0.998 8)。用该方法测定了母乳脂肪、实验室合成结构油脂样品、商品结构油脂、棕榈油和山茶油中OPO的含量。结果表明该方法的灵敏度高、重现性良好。

Selective adsorption of silver ions from aqueous solution using polystyrene-supported trimercaptotriazine resin

Tdmercaptotriazine-functionalized polystyrene chelating resin was prepared and employed for the adsorption of Ag（I） from aqueous solution. The adsorbent was characterized according to the following techniques： Fourier transform infrared spectroscopy, elemental analysis, scanning electron microscopy and the Brunauer-Emmet-Teller method. The effects of initial Ag（I） concentration, contact time, solution pH and coexisting ions on the adsorption capacity of Ag（I） were systematically investigated. The maximum adsorption capacity of AgO） was up to 187.1 mg/g resin at pH 0.0 and room temperature. The kinetic experiments indicated that the adsorption rate of Ag（I） onto the chelating resin was quite fast in the first 60 rain and reached adsorption equilibrium after 360 min. The adsorption process can be well described by the pseudo second-order kinetic model and the equilibrium adsorption isotherm was closely fitted by the Langmuir model. Moreover, the chelating resin could selectively adsorb more Ag（I） ions than other heavy metal ions including： Cu（Ⅱ）, Zn（Ⅱ）, Ni（Ⅱ）, Pb（Ⅱ） and Cr（Ⅲ） during competitive adsorption in the binary metal species systems, which indicated that it was a highly selective adsorbent of Ag（I） from aqueous solution.

Deep Desulfurization via Adsorption by Silver Modified Bentonite

In order to further reduce the sulfur content in liquid hydrocarbon fuels,a desulfurization process by adsorption for removing alkyl dibenzothiophenes was investigated.Desulfurization of model gasoline by bentonite adsorbents loaded with silver nitrate was studied.The test results indicated that the bentonite adsorbents loaded with Ag + ions were effective for adsorbing the alkyl dibenzothiophenes.The crystal structure of bentonite adsorbents was characterized by X-ray diffraction (XRD) and their acidity was measured by Fourier transform infrared (FT-IR) spectroscopy.Several factors influencing the desulfurization capability,including the Ag + loading,the baking temperature,as well as the reaction temperature,were investigated.The desulfurization efficiency was enhanced by increasing the Ag + loading and the best result was obtained at a silver loading of 7 m%.It was found that the adsorption capacity of the alkyl dibenzothiophenes on bentonite loaded with Ag + ions increased with a decreasing temperature.Baking of the adsorbent could also improve the desulfurization capacity,and the optimum baking temperature was 423 K.

Sunlight-driven reduction of silver ion to silver nanoparticle by organic matter mitigates the acute toxicity of silver to Daphnia magna

Due to the unique antibacterial activities, silver nanoparticles（AgNPs） have been extensively used in commercial products. Anthropogenic activities have released considerable AgNPs as well as highly toxic silver ion（Ag^＋） into the aquatic environment.Our recent study revealed that ubiquitous natural organic matter（NOM） could reduce Ag^＋to Ag NP under natural sunlight. However, the toxic effect of this process is not well understood. In this work, we prepared mixture solution of Ag^＋and AgNPs with varied Ag^＋% through the sunlight-driven reduction of Ag^＋by NOM and investigated the acute toxicity of the solutions on Daphnia magna. Formation of AgNPs was demonstrated and characterized by comprehensive techniques and the fraction of unconverted Ag^＋was determined by ultrafiltration-inductively coupled plasma mass spectrometry determination. The formation of AgNPs enhanced significantly with the increasing of solution p H and cumulative photosynthetically active radiation of sunlight. The toxicity of the resulting solution was further investigated by using freshwater crustacean D. magna as a model and an 8 hr-median lethal concentration（LC50） demonstrated that the reduction of Ag^＋by NOM to AgNPs significantly mitigated the acute toxicity of silver. These results highlight the importance of sunlight and NOM in the fate, transformation and toxicity of Ag^＋and AgNPs,and further indicate that the acute toxicity of AgNPs should be mainly ascribed to the dissolved Ag^＋from AgNPs.

Comparative toxicity of silver nanoparticles and silver ions to Escherichia coli

With the increase in silver（Ag）-based products in our lives, it is essential to test the potential toxicity of silver nanoparticles（Ag NPs） and silver ions（Ag ions） on living organisms under various conditions. Here, we investigated the toxicity of Ag NPs with Ag ions to Escherichia coli K-12 strain under various conditions. We observed that both Ag NPs and Ag ions display antibacterial activities, and that Ag ions had higher toxicity to E. coli K-12 strain than Ag NPs under the same concentrations. To understand the toxicity of Ag NPs at a cellular level, reactive oxygen species（ROS） enzymes were detected for use as antioxidant enzymatic biomarkers. We have also studied the toxicity of Ag NPs and Ag ions under various coexistence conditions including： fixed total concentration, with a varied the ratio of Ag NPs to Ag ions; fixed the Ag NPs concentration and then increased the Ag ions concentration; fixed Ag ions concentration and then increasing the Ag NPs concentration.Exposure to Ag NPs and Ag ions clearly had synergistic toxicity; however, decreased toxicity（for a fixed Ag NPs concentration of 5 mg/L, after increasing the Ag ions concentration） to E. coli K-12 strain. Ag NPs and Ag ions in the presence of L-cysteine accelerated the bacterial cell growth rate, thereby reducing the bioavailability of Ag ions released from Ag NPs under the single and coexistence conditions. Further works are needed to consider this potential for Ag NPs and Ag ions toxicity across a range of environmental conditions.Environmental Significance Statement： As silver nanoparticles（Ag NPs）-based products are being broadly used in commercial industries, an ecotoxicological understanding of the Ag NPs being released into the environment should be further considered. Here, we investigate the comparative toxicity of Ag NPs and silver ions（Ag ions） to Escherichia coli K-12 strain, a representative ecotoxicological bioreporter. This study showed that toxicities of Ag NPs and Ag ions to E. coli K-12 strain display different relationships when existing individually or when coexisting, and in the presence of L-cysteine materials. These findings suggest that the toxicology research of nanomaterials should consider conditions when NPs coexist with and without their bioavailable ions.

A Novel Highly Selective“Turn-On”Fluorescence Sensor for Silver Ions Based on Schiff Base

A novel optical chemical sensor L was designed and synthesized for the determination of silver ions.The sensor L was derived from 1-naphthaldehyde and 3,4,5-tris(hexadecyloxy)benzohydrazide.The sensor L shows high sensitivity and selectivity for Ag+detection in comparison to other metal cations(Mg^(2+),Ca^(2+),Al^(3+),Cr^(3+),Fe^(3+),Co^(2+),Ni^(2+),Cu^(2+),Zn^(2+),Cd^(2+),Hg^(2+),Pb^(2+))and has no significant response to other common metal cations.Upon addition of Ag+,the fluorescent emission of the sensor L was enhanced dramatically but other metal cations had no same response.The detection limit for Ag+was 1.2×10^(−7) mol/L.Therefore,the sensor L is useful for Ag+detection with some advantages including sensitivity,selectivity,simplicity and low-cost.

Photocatalytic Inhibition of Cyanobacterial Growth Using Silver-doped TiO_2 under UV-C Light

Capacity of the silver-doped TiO2 under UV-C light to restrain cyanobacterial growth was explored with Anabaena sp. PCC 7120 and Microcystis aeruginosa as test species. The survival, chlorophyll bleaching, photosynthetic activity, DNA breakages, antioxidant enzyme activities, lipid peroxidation, and cellular morphologic structure of test cyanobacteria were analyzed. The results indicate that the test cyanobacteria with UV-C photocatalysis by silver-doped TiO2 sufferd from effects of reactive oxygen species, which promote the damage of the cell wall and the peroxidation of cell membranes, and subsequently, aggravate the losses of cell organelle and viability. The results suggest that UV-C photocatalysis by the silver ions doped TiO2 could be a promising method to prevent fast and excessive growth of cyanobacteria in eutrophic water sources.

Synthesis of polymer protected Au NPs for silver ions detection

Herein, a novel probe based on poly(N,N′-methylenebisacylamide) protected Au NPs(PDMAM-Au NPs) was developed for determination of silver ions. The thiol-terminated PDMAM was synthesized by the reversible addition fragmentation chain transfer radical polymerization. Then, the PDMAM-Au NPs were prepared by a simple, one-pot and green process. It has been observed that existence of the silver ions evoked a small red-shift to the emission peak of PDMAM-Au NPs and an obvious increase in UV absorption. The detection limit of the present method for silver ions was 0.5 ?mol/L, with a linear range of 1.0–1000 ?mol/L. Moreover, the possible mechanism for enhanced UV absorption intensity with enlarged diameter of PDMAM-Au NPs was explored and discussed briefly. The results demonstrated that silver ions could be detected by the PDMAM-Au NPs based colorimetric assays, further opening up new ways for the visualization and quantification of silver ions involved in our daily life.

Silver Ion-Induced Formation of Unprecedented Thorium Nonamer Clusters via Lacuna-Construction Strategy

Herein,we report the synthesis and structures of two novel mixed-metal clusters denoted as Th_(9)Ag_(6)and Th_(9)Ag_(12).Both clusters feature unprecedented Th_(9)cores.The cores are tricapped trigonal prism moieties that are novel among actinides.Attempted alternative synthesis routes indicate that the Th_(9)clusters are accessible only through slow introduction of Ag_(+)into a solution containing a Th6 cluster modified with 2-picolinic acid.Alternative rapid addition of Ag_(+)leads to dissociation of the Th6 cluster with formation of a high-purity(ThAg)_(∞)two-dimensional layered structure material.A mechanism for cluster dissociation and reassembly to yield Th_(9)from Th6 is proposed that is consistent with spectroscopic observations and computational results.Because of Ag⋯Ag andπ–πinteractions,the Th_(9)Ag_(12)cluster exhibits high stability in air,at elevated temperature,underγ-irradiation,and in common solvents.

Target-triggered inhibiting oxidase-mimicking activity of platinum nanoparticles for ultrasensitive colorimetric detection of silver ion

The development of efficient methods for the detection of hazardous and toxic elements is extremely important for environmental security and public health. In this work, we developed a facile colorimetric assaying system for Ag+ detection in aqueous solution. Chitosan-stabilized platinum nanoparticles(ChPtNPs) were synthesized and severed as an artificial oxidase to catalyze the oxidation of the substrate3,30,5,50-tetramethylbenzidine(TMB) and generate color signal. In the presence of Ag+, due to the strong metallophilic interactions between Ag+ and Pt2+ on the surface of Ch-PtNPs, Ag+ can weaken the affinity to the substrates and inactivate the catalytic activity of Ch-PtNPs, leading to decreased absorbance signal to varying degrees depending on Ag+ amount. Combing the specific binding between Ch-PtNPs and Ag+ with signal amplification procedure based on the Ch-PtNPs-catalyzed TMB oxidation, a sensitive,selective, simple, cost-effective, and rapid detection method for Ag+ can be realized. Ag+ ions in tap and lake waters have been successfully detected. We ensured that the proposed method can be a potential alternative for Ag+ determination in environmental samples.

A Novel Method for the Selective Determination of Silver (I) Ion

Water-soluble CdSe quantum dots (QDs) capped by bovine serum albumin (BSA) is described as selective silver (I) ion probe based on their fluorescence (FL) quenched by silver ion at pH 5.7. The detection limit is 3×10-7 mol L-1 for silver ion. The interference was also investigated.

Ground-based Investigations of Atomic Oxygen Erosion Behaviors of Silver and Ion-implanted Silver

Silver foils and ion-implanted silver foils exposed to atomic oxygen （AO） generated in a ground simulation facility were investigated by the quartz crystal microbalance （QCM）, the scanning electron microscopy （SEM） and the X-ray photoelectron spectroscopy （XPS）. The experimental results show the presence of Ag2O and AgO in an oxidation process of the silver foil having exposure to AO. As soon as silver comes under the bombardment of atomic oxygen, the oxidation process starts with a thick film forming on the silver surface. Because of the development of stresses, the oxide layer gets cracked and spalled, which leads to appearance of a new silver surface intensifying further oxidation. At last, AgO begins to form on the outer surface of the oxide film. The analytical results of the XPS and the AES attest to formation of a continuous high-quality protective oxide-based layer on the surface of ion-implanted silver films after exposure to AO. This layer can well protect materials in question from erosion.

A comparative study of four 20-membered N_2S_4-crown ethers as ionophores for polymeric membrane silver selective electrodes

Four 20-membered N_2S_4-monoazathiacrown ethers have been synthesized and explored as neutral ionophores for Ag^+-selective electrodes.Potentiometric responses reveal that the flexibility of the ligands has great effect on the selectivity and sensitivity to Ag^+ ions.The electrode based on ionophore 9,10,20,25-tetrahydro-5H,12H-tribenzo[b,n,r][1,7,10,16,4,13]tetrathiadiaza cycloicosine 6,13-(7H,14H)-dione(C) with 2-nitrophenyl octyl ether(o-NPOE) as solvent in a poly(vinyl chloride)(PVC) membrane matrix sho...

Photo-Induced DNA-Dependent Conformational Changes in Silver Nanoparticles

The goal of the present investigation is to study the interaction of AgNPs with thymus DNA by traditional and original spectra-photometric and thermo-dynamic methods and approaches in darkness and under photo-irradiation. 1) At the interaction of DNA with nano-particles hypsochromic shift of 6 nm and hypochromic effect of 20% of AgNPs absorption band are observed. 2) At photo-irradiation (λ = 436 nm or full spectrum of visible band) of AgNPs-DNA complexes absorption spectra band width is changed from 140 nm to 360 nm at half-height. Besides, isosbestic point is observed. 3) Kinetic study of photo-diffusion has made it possible to determine desorption rate constant and desorption reaction activation energy that are equal respectively to kd @ 9 ′ 10-5 s-1, Ed @ 80 kJ/M Ag0 for AgNPs bound with DNA. It is shown that AgNPs represent liquid drops which moisture the DNA surface at interaction. At photo-irradiation of AgNPs-DNA complex DNA dependant conformational transition takes place due to fast and intensive heating.

In vitro microbiologically-induced concrete corrosion behavior of Ag^(+) loaded zeolite-polyurethane coating for concrete sewer applications

Microbiologically-induced concrete corrosion(MICC)refers to chemical reactions between biologically produced sulphuric acid and with hydration products in the hardened concrete paste,resulting in an early reduction of strength,deterioration,and very severe circumstances,structural failure.This paper explores the bactericidal characteristics of cementitious materials with surface coated with modified zeolite-polyurethane.The zeolite-polyurethane coating incorporated with silver was studied in environments inoculated with A.thiooxidans bacteria for 8 consecutive weeks.The antibacterial characteristics were evaluated in terms of pH,optical density(OD),sulphate production and bacteria count to determine the effectiveness of the zeolite-polyurethane coatings in environments inoculated with A.thiooxidans bacteria producing the sulphuric acid.The results revealed that the samples incorporated with silver modified zeolites generally showed antibacterial performance(regardless of the zeolite type)compared with unmodified polyurethane coating.This was evaluated by the lack of bacteria attachment and the deformed microcolonies on the sample surface,lag in pH reduction,increase in OD,and sulphate production.The silver zeolites gained their antibacterial performance from the release of silver ions(Ag^(+))when the sample comes into contact with aqueous solutions.This results in the inhibition of cell functions of the bacteria and subsequently causes cell damage.