The present work studied the application of AgNOR count to differential diagnosis between cutaneous T cell lymphoma (CTCL) and cutaneous pseudolymphoma (CPL). Paraffin sections from 50 mycosis fungoides (22 MFI-Premyc...The present work studied the application of AgNOR count to differential diagnosis between cutaneous T cell lymphoma (CTCL) and cutaneous pseudolymphoma (CPL). Paraffin sections from 50 mycosis fungoides (22 MFI-Premycotic stage, 24 MF Ⅰ infiltrative stage and 4 MF Ⅲ - tumor stage), 2 nonepidermotropic cutaneous T cell lymphoma (NECTCL) and 9 CPL were investigated. In each case, 200 cells randomly selected were examined using a × 100 oil immersion lens. The mean number, standard deviation and standard error of the mean of AgNOR counts were as follows: MFⅠ 1.17±0.09, SEM = 0.01; MⅡ 1.17±0.01, SEM = 0.01; MF Ⅲ. 3.55±0.87, SEM = 0.43; NECTCL 4.5±0.28, SEM -0.199; CPL 1.17±0.1, SEM ± 0.03. The results revealed a highly significant difference between CTCL (MFⅢ+NECTCL) and CPL (t = 4.75, P<0.001), tumor stage (MF Ⅲ) and pretumor stage (MFI, MF Ⅱ) of mycosis fungoides (t = 4.75, P<0.001). Thus. AgNOR count is valuable in differential diagnosis.展开更多
The argyrophil method for nucleolar organizer regions (AgNOR) known as the AgNor technique and mucin histochemical stain were applied to Investigate the dysplasia and cancaration in ulcerative colitis Including 58 cas...The argyrophil method for nucleolar organizer regions (AgNOR) known as the AgNor technique and mucin histochemical stain were applied to Investigate the dysplasia and cancaration in ulcerative colitis Including 58 cases of biopsy specimens and three cases operative specimens. The numbers of AgNOR have been gradully increased with the grades of dasplasls. Similarly the percentage of sialomucin having vicinal diols on side chain of sialic acid also gradully Increased with the grades of dysplasia. The AgNOR reflects rDNA transcriptional activity responsible for degrees of differentiation of cell. Epithelial cells secreting a heterogeneous mucin, could be taken as a signal of abnormal cellular differentiation. AgNOR and mucln chages might be assumed as a criteria In representing malignant transformation.展开更多
Objective:To evaluate the relation between argyrophilic nucleolar organizer region(Ag NOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer(NSCLC).Methods:A total of 20...Objective:To evaluate the relation between argyrophilic nucleolar organizer region(Ag NOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer(NSCLC).Methods:A total of 207 surgical specimens diagnosed as NSCLC were included in this study.Double-staining procedures were performed using antigen Ki-67(clone MIB-1)and silver nitrate by immunohistochemical and Ag NOR-staining methods.Results:The Ag NOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM(tumor,node,and metastasis)system.The survival of patients with small Ag NOR area in MIB-1-positive cells is better than that of patients with large Ag NOR area.Molecular,biological(Ag NOR area in MIB-1-positive cells),and clinicopathological(greatest tumor dimension,metastases to regional lymph nodes,histology,and differentiation)parameters are independent prognostic factors of NSCLC.Conclusion:The Ag NOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.展开更多
NOR is a chromosomal segment which contains several copies of the genes coding for 18 s and 28 s rNRA. This segment is found in humans on the secondary constriction site of the acrocentric chromosome (AC). A variant o...NOR is a chromosomal segment which contains several copies of the genes coding for 18 s and 28 s rNRA. This segment is found in humans on the secondary constriction site of the acrocentric chromosome (AC). A variant of NOR, double NOR (dNOR), has been found in some people. Deletion, repetition and elongated variants have also been found in previous studies. Light microscopic observations and satellite association (SA) silver staining techniques were used to measure展开更多
用银胶染色技术,对15例前列腺癌的石蜡切片标本进行了银染色核仁组成区的定量研究。以15例前列腺增生和10例良性增生伴上皮增生显著病例作对照。结果表明,前列腺癌胞核内 NORs 数增多,其均值为5.05±1.49(±S),显著高于前列...用银胶染色技术,对15例前列腺癌的石蜡切片标本进行了银染色核仁组成区的定量研究。以15例前列腺增生和10例良性增生伴上皮增生显著病例作对照。结果表明,前列腺癌胞核内 NORs 数增多,其均值为5.05±1.49(±S),显著高于前列腺增生的2.04±0.53(±S)和良性增生伴上皮增生显著的2.08±0.51(±S),P<0.001。作者认为,Ag-NOR 技术对前列腺良恶性病变的鉴别诊断有一定意义。展开更多
文摘The present work studied the application of AgNOR count to differential diagnosis between cutaneous T cell lymphoma (CTCL) and cutaneous pseudolymphoma (CPL). Paraffin sections from 50 mycosis fungoides (22 MFI-Premycotic stage, 24 MF Ⅰ infiltrative stage and 4 MF Ⅲ - tumor stage), 2 nonepidermotropic cutaneous T cell lymphoma (NECTCL) and 9 CPL were investigated. In each case, 200 cells randomly selected were examined using a × 100 oil immersion lens. The mean number, standard deviation and standard error of the mean of AgNOR counts were as follows: MFⅠ 1.17±0.09, SEM = 0.01; MⅡ 1.17±0.01, SEM = 0.01; MF Ⅲ. 3.55±0.87, SEM = 0.43; NECTCL 4.5±0.28, SEM -0.199; CPL 1.17±0.1, SEM ± 0.03. The results revealed a highly significant difference between CTCL (MFⅢ+NECTCL) and CPL (t = 4.75, P<0.001), tumor stage (MF Ⅲ) and pretumor stage (MFI, MF Ⅱ) of mycosis fungoides (t = 4.75, P<0.001). Thus. AgNOR count is valuable in differential diagnosis.
文摘The argyrophil method for nucleolar organizer regions (AgNOR) known as the AgNor technique and mucin histochemical stain were applied to Investigate the dysplasia and cancaration in ulcerative colitis Including 58 cases of biopsy specimens and three cases operative specimens. The numbers of AgNOR have been gradully increased with the grades of dasplasls. Similarly the percentage of sialomucin having vicinal diols on side chain of sialic acid also gradully Increased with the grades of dysplasia. The AgNOR reflects rDNA transcriptional activity responsible for degrees of differentiation of cell. Epithelial cells secreting a heterogeneous mucin, could be taken as a signal of abnormal cellular differentiation. AgNOR and mucln chages might be assumed as a criteria In representing malignant transformation.
文摘Objective:To evaluate the relation between argyrophilic nucleolar organizer region(Ag NOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer(NSCLC).Methods:A total of 207 surgical specimens diagnosed as NSCLC were included in this study.Double-staining procedures were performed using antigen Ki-67(clone MIB-1)and silver nitrate by immunohistochemical and Ag NOR-staining methods.Results:The Ag NOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM(tumor,node,and metastasis)system.The survival of patients with small Ag NOR area in MIB-1-positive cells is better than that of patients with large Ag NOR area.Molecular,biological(Ag NOR area in MIB-1-positive cells),and clinicopathological(greatest tumor dimension,metastases to regional lymph nodes,histology,and differentiation)parameters are independent prognostic factors of NSCLC.Conclusion:The Ag NOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.
文摘NOR is a chromosomal segment which contains several copies of the genes coding for 18 s and 28 s rNRA. This segment is found in humans on the secondary constriction site of the acrocentric chromosome (AC). A variant of NOR, double NOR (dNOR), has been found in some people. Deletion, repetition and elongated variants have also been found in previous studies. Light microscopic observations and satellite association (SA) silver staining techniques were used to measure
文摘用银胶染色技术,对15例前列腺癌的石蜡切片标本进行了银染色核仁组成区的定量研究。以15例前列腺增生和10例良性增生伴上皮增生显著病例作对照。结果表明,前列腺癌胞核内 NORs 数增多,其均值为5.05±1.49(±S),显著高于前列腺增生的2.04±0.53(±S)和良性增生伴上皮增生显著的2.08±0.51(±S),P<0.001。作者认为,Ag-NOR 技术对前列腺良恶性病变的鉴别诊断有一定意义。
