In this paper, the model of the online real-time information transmission network, such as wechat, micro-blog, and QQ network, is proposed and built, based on the connection properties between users of the online real...In this paper, the model of the online real-time information transmission network, such as wechat, micro-blog, and QQ network, is proposed and built, based on the connection properties between users of the online real-time information transmission network, and combined with the local world evolving characteristics in complex network, then the statistical topological properties of the network is obtained by numerical simulation. Furthermore, we simulated the process of information transmission on the network, according to the actual characteristics of the online real-time information transmission. Statistics show that the degree distribution presents the characteristics of scale free network, presenting power law distribution, while the average path length, the average clustering coefficient and the average size of the network also has a power-law relationship, moreover, the model parameters has no effect on power-law exponent. The spread of information on the network represents obvious fluctuation scaling, reflecting the characteristics that information transmission fluctuates over time.展开更多
To evaluate and improve the real-time performance of Ethernet for plant automation(EPA) industrial Ethernet,the real-time performance of EPA periodic data transmission was theoretically and experimentally studied.By...To evaluate and improve the real-time performance of Ethernet for plant automation(EPA) industrial Ethernet,the real-time performance of EPA periodic data transmission was theoretically and experimentally studied.By analyzing information transmission regularity and EPA deterministic scheduling mechanism,periodic messages were categorized as different modes according to their entering-queue time.The scheduling characteristics and delivery time of each mode and their interacting relations were studied,during which the models of real-time performance of periodic information transmission in EPA system were established.On this basis,an experimental platform is developed to test the delivery time of periodic messages transmission in EPA system.According to the analysis and the experiment,the main factors that limit the real-time performance of EPA periodic data transmission and the improvement methods were proposed.展开更多
Distributed speech recognition (DSR) applications have certain QoS (Quality of service) requirements in terms of latency, packet loss rate, etc. To deliver quality guaranteed DSR application over wirelined or wireless...Distributed speech recognition (DSR) applications have certain QoS (Quality of service) requirements in terms of latency, packet loss rate, etc. To deliver quality guaranteed DSR application over wirelined or wireless links, some QoS mechanisms should be provided. We put forward a RTP/RSVP transmission scheme with DSR-specific payload and QoS parameters by modifying the present WAP protocol stack. The simulation result shows that this scheme will provide adequate network bandwidth to keep the real-time transport of DSR data over either wirelined or wireless channels.展开更多
For the convenience of people with disability and for normal people, a demand for intelligent interfaces is ever increasing and therefore related studies are actively being conducted. Recently a study is being conduct...For the convenience of people with disability and for normal people, a demand for intelligent interfaces is ever increasing and therefore related studies are actively being conducted. Recently a study is being conducted to develop an interface through face expression, movement of the body and eye movements, and further more active attempts to use electrical signals(brainwave, electrocardiogram, electromyogram) measured from the human body is also actively being progressed. In addition, the development and the usage of mobile devices and smart devices are promoting these research activities even more. The brainwave is measured by electrical activities between nerve cells in the cerebral cortex using scalp electrodes. The brainwave is mainly used for diagnosis and treatment of diseases such as epilepsy, encephalitis, brain tumors and brain damage. As a result, the brainwave measurement methods and analytical methods were developed. Interface using the brainwave will not go through language or body behavior which is the result of the information processed by the brain but will pass directly to the system providing a brain-computer interface (BCI). This is possible because a variety of the brainwave appears depending on the human’s physical and mental state. Using the brainwave with the intelligent brain-computer interface or combining it with mobile devices and smart devices, regardless of space constraints, the brainwave measurement should be possible.[4,7] In this study, in order to measure the brainwave without spatial constraint, 16 channel compact brainwave measurements system using a high-speed wireless communications were designed. It was designed with a 16 channel to classify the various brainwave patterns that appear and for estimating the location of the nerve cells that triggered the brainwave. And in order to transmit the brainwave data within the channel without loss, a high-speed wireless communication must be possible that can enable a high-speed wireless transmission more sufficient than the Bluetooth, therefore, 802.11 compliant Wi-Fi communication methods were used to transfer the data to the PC. In addition, by using an analog front-end IC having a single-chip configuration with real-time digital filters, the miniaturization of the system was implemented and in order to verify the system Eye-blocking was used to observe the changes in the EEG signal.展开更多
[Objective] This study aimed to establish a TaqMan-based real-time PCR assay for detecting transmissible gastroenteritis virus (TGEV). [Method] Primers and a probe were designed according to the conserved sequence o...[Objective] This study aimed to establish a TaqMan-based real-time PCR assay for detecting transmissible gastroenteritis virus (TGEV). [Method] Primers and a probe were designed according to the conserved sequence of N gene in TGEV genome. After gradient dilution, the recombinant plasmid harboring the N gene was used as a standard for real-time PCR assay to establish the standard curve. [Re- sult] The results showed that the established real-time PCR assay exhibited a good linear relationship within the range of 102-10^10 copies/ul; the correlation coefficient was above 0.99 and the amplification efficiency ranged from 90% to 110%. The de- tection limit of real-time PCR assay for TGEV was 10 copies/μl, suggesting a high sensitivity; there was no cross reaction with other porcine viruses, indicating a good specificity; coefficients of variation within and among batches were lower than 3%, suggesting a good repeatability. The established real-time PCR method could be ap- plied in quantitative analysis and evaluation of the immune efficacy of TGEV vac- cines and detection of TGEV in clinical samples. [Conclusion] The TaqMan-based real-time PCR assay established in this study is highly sensitive and specific, which can provide technical means for the epidemiological survey of TGEV, development of TGEV vaccines and investigation of the pathogenesis of TGE.展开更多
This work presents an optimal design method of antenna aperture illumination for microwave power transmission with an annular collection area.The objective is to maximize the ratio of the power radiated on the annular...This work presents an optimal design method of antenna aperture illumination for microwave power transmission with an annular collection area.The objective is to maximize the ratio of the power radiated on the annular collection area to the total transmitted power.By formulating the aperture amplitude distribution through a summation of a special set of series,the optimal design problem can be reduced to finding the maximum ratio of two real quadratic forms.Based on the theory of matrices,the solution to the formulated optimization problem is to determine the largest characteristic value and its associated characteristic vector.To meet security requirements,the peak radiation levels outside the receiving area are considered to be extra constraints.A hybrid grey wolf optimizer and Nelder–Mead simplex method is developed to deal with this constrained optimization problem.In order to demonstrate the effectiveness of the proposed method,numerical experiments on continuous apertures are conducted;then,discrete arrays of isotropic elements are employed to validate the correctness of the optimized results.Finally,patch arrays are adopted to further verify the validity of the proposed method.展开更多
Abts ract A wireless mutl i-hop videot ransmission experiment system is designed and implemented for vehiculra ad-hoc networks VANET and the rt ansm ission control protocol and routing protocol are proposed. This syst...Abts ract A wireless mutl i-hop videot ransmission experiment system is designed and implemented for vehiculra ad-hoc networks VANET and the rt ansm ission control protocol and routing protocol are proposed. This system in tegrates the embedded Linux system witha n ARM kernel and oc ns ists of a S3C6410 main control module a wirel ss local arean etwork WLAN card a LCD screne and so on.In the scenario of a wireless multi-hop video transmission both the H.264 and JPEG are used and their performances such as the compression rate delay and frame loss rate are analyzed in theory andc ompared in the experiment.The system is tested in the real indoor and outdoor environment.The results show that the scheme of the multi-hop video transmission experiment system can be applicable for VANET and multiple scenes and the transmission control protocol and routing protocol proposed can achieve real-time transmission and meet multi-hop requirements.展开更多
A rapidly deployable dense seismic monitoring system which is capable of transmitting acquired data in real time and analyzing data automatically is crucial in seismic hazard mitigation after a major earthquake.Howeve...A rapidly deployable dense seismic monitoring system which is capable of transmitting acquired data in real time and analyzing data automatically is crucial in seismic hazard mitigation after a major earthquake.However,it is rather difficult for current seismic nodal stations to transmit data in real time for an extended period of time,and it usually takes a great amount of time to process the acquired data manually.To monitor earthquakes in real time flexibly,we develop a mobile integrated seismic monitoring system consisting of newly developed nodal units with 4G telemetry and a real-time AI-assisted automatic data processing workflow.The integrated system is convenient for deployment and has been successfully applied in monitoring the aftershocks of the Yangbi M_(S) 6.4 earthquake occurred on May 21,2021 in Yangbi County,Dali,Yunnan in southwest China.The acquired seismic data are transmitted almost in real time through the 4G cellular network,and then processed automat-ically for event detection,positioning,magnitude calculation and source mechanism inversion.From tens of seconds to a couple of minutes at most,the final seismic attributes can be presented remotely to the end users through the integrated system.From May 27 to June 17,the real-time system has detected and located 7905 aftershocks in the Yangbi area before the internal batteries exhausted,far more than the catalog provided by China Earthquake Networks Center using the regional permanent stations.The initial application of this inte-grated real-time monitoring system is promising,and we anticipate the advent of a new era for Real-time Intelligent Array Seismology(RIAS),for better monitoring and understanding the subsurface dynamic pro-cesses caused by Earth's internal forces as well as anthropogenic activities.展开更多
Multi-channel can be used to provide higher transmission ability to the bandwidth-intensive and delay-sensitive real-time streams. However, traditional channel capacity theories and coding schemes are seldom designed ...Multi-channel can be used to provide higher transmission ability to the bandwidth-intensive and delay-sensitive real-time streams. However, traditional channel capacity theories and coding schemes are seldom designed for the real-time streams with strict delay constraint, especially in multi-channel context. This paper considers a real-time stream system, where real-time messages with different importance should be transmitted through several packet erasure channels, and be decoded by the receiver within a fixed delay. Based on window erasure channels and i.i.d.(identically and independently distributed) erasure channels, we derive the Multi-channel Real-time Stream Transmission(MRST) capacity models for Symmetric Real-time(SR) streams and Asymmetric Real-time(AR) streams respectively. Moreover, for window erasures, a Maximum Equilibrium Intra-session Code(MEIC) is presented for SR and AR streams, and is shown able to asymptotically achieve the theoretical MRST capacity. For i.i.d. erasures, we propose an Adaptive Maximum Equilibrium Intra-session Code(AMEIC), and then prove AMEIC can closely approach the MRST transmission capacity. Finally, the performances of the proposed codes are verified by simulations.展开更多
Accuracy measurement of the Non-soluble Deposit Density (NSDD) on the insulator surface is very important for the transmission line anti-pollution flashover works. A method to measure the NSDD on double sheds porcelai...Accuracy measurement of the Non-soluble Deposit Density (NSDD) on the insulator surface is very important for the transmission line anti-pollution flashover works. A method to measure the NSDD on double sheds porcelain insulator surface based on laser transmission principle is proposed in this paper. Laser unit and luminous intensity sensor are installed between the up and down surface of the double sheds porcelain insulators, two glass tablets are put between the double sheds. The contamination on the glass tablets will influence the luminous intensity that reaches the intensity sensor. The luminous signal is changed to electrical signal, and the insulator’s NSDD could be obtained based on the difference of luminous intensity. The device can be used in online monitoring of the insulator's NSDD condition on the insulator surface.展开更多
In more than half of infertile men, the cause of their infertility is unknown. Several studies revealed the role of viral infections in male infertility. The aim of the present study was to determine the prevalence of...In more than half of infertile men, the cause of their infertility is unknown. Several studies revealed the role of viral infections in male infertility. The aim of the present study was to determine the prevalence of herpes simplex virus-1 (HSV-1) and HSV-2 in semen from asymptomatic infertile male patients, and its association with altered semen parameters. A total of 70 semen samples were collected from infertile men who attended the Research and Clinical Center for Infertility in Yazd, Iran. Semen analysis and diagnostic real-time PCR using specific primers and probes for HSV-1 and HSV-2 DNA were performed. Comparison of semen parameters between virally in- fected and non-infected samples were performed with independent t-test and Mann-Whitney test. Semen analysis showed that infertile men fell into two groups, the male factor group and the unexplained group. HSV-1 and HSV-2 DNA was detected in 16 (22.9%) and 10 (14.3%) of 70 semen samples, respectively. All HSV-positive samples had abnormal semen parameters (the male factor group). Although HSV infection was not associated with sperm motility and morphological defects, it was correlated with lower sperm count in the seminal fluid. The findings suggest that asymptomatic seminal infection of HSV plays an important role in male infertility by adversely af- fecting sperm count.展开更多
Objective: To explore the functions and mechanisms of herpes simplex virus type I(HSV-1) while infecting human oral epithelial cells in vitro(being similar to the infection in vivo). Methods:An abundance of HSV-...Objective: To explore the functions and mechanisms of herpes simplex virus type I(HSV-1) while infecting human oral epithelial cells in vitro(being similar to the infection in vivo). Methods:An abundance of HSV-1 strains amplified in Vero cells were used to infect human oral epithelial cells. The culture supernatant was collected to infect Vero cells again. Morphology of HSV-1 was identified by inverted microscope and transmission electron microscope. Nucleic acid of the virus was detected by PCR. Results:The infected human oral epithelial cells didn' t display an obvious cytopathic effect(CPE) under inverted microscope(while Vero cells which were infected by the culture supernatant showed typical(CPE). The virus particles were not observed in the cytoplasm nor in nucleus of human oral epithelial cells, however under transmission electron microscope in the cytoplasm of Vero cells, the nucleic acid of HSV-1 could be detected in infected human oral epithelial cells, by PCR. Conclusion-HSV-1 can successfully infect human oral epithelial cells. This model may provide a useful approach for studying the pathogenesis of herpes virus-associated periodontal disease.展开更多
In-situ observation of the growth and decomposition processes is significantly important for guiding the fabrication of upconversion nanocrystals(UCNCs)with high performance.However,the high crystallization energy and...In-situ observation of the growth and decomposition processes is significantly important for guiding the fabrication of upconversion nanocrystals(UCNCs)with high performance.However,the high crystallization energy and rapid nucleation rate of the corresponding crystals make real-time observation still a huge challenge.Herein,the in-situ nucleation-growth-degradation processes of the UCNCs are investigated by employing tailored metastable intermediates,which possess a slowing-down nucleation rate under electron beam irradiation.The non-classical nucleation processes of the UCNCs,containing the coalescence of clusters and the subsequent crystallization,are demonstrated.Moreover,the Ostwald ripening and oriented attachment processes,which determine the particle size and morphology,are unambiguously recorded.Furthermore,the degrading process of the UCNCs is observed to be triggered by surface defects.Our work provides an insight into the real-time evolution dynamics of the UCNCs,which further sheds light on the fabrication of nano-sized up-conversion phosphors.展开更多
文摘In this paper, the model of the online real-time information transmission network, such as wechat, micro-blog, and QQ network, is proposed and built, based on the connection properties between users of the online real-time information transmission network, and combined with the local world evolving characteristics in complex network, then the statistical topological properties of the network is obtained by numerical simulation. Furthermore, we simulated the process of information transmission on the network, according to the actual characteristics of the online real-time information transmission. Statistics show that the degree distribution presents the characteristics of scale free network, presenting power law distribution, while the average path length, the average clustering coefficient and the average size of the network also has a power-law relationship, moreover, the model parameters has no effect on power-law exponent. The spread of information on the network represents obvious fluctuation scaling, reflecting the characteristics that information transmission fluctuates over time.
基金Supported by the National High Technology Research and Development Program of China (2006AA040301-4,2007AA041301-6)
文摘To evaluate and improve the real-time performance of Ethernet for plant automation(EPA) industrial Ethernet,the real-time performance of EPA periodic data transmission was theoretically and experimentally studied.By analyzing information transmission regularity and EPA deterministic scheduling mechanism,periodic messages were categorized as different modes according to their entering-queue time.The scheduling characteristics and delivery time of each mode and their interacting relations were studied,during which the models of real-time performance of periodic information transmission in EPA system were established.On this basis,an experimental platform is developed to test the delivery time of periodic messages transmission in EPA system.According to the analysis and the experiment,the main factors that limit the real-time performance of EPA periodic data transmission and the improvement methods were proposed.
文摘Distributed speech recognition (DSR) applications have certain QoS (Quality of service) requirements in terms of latency, packet loss rate, etc. To deliver quality guaranteed DSR application over wirelined or wireless links, some QoS mechanisms should be provided. We put forward a RTP/RSVP transmission scheme with DSR-specific payload and QoS parameters by modifying the present WAP protocol stack. The simulation result shows that this scheme will provide adequate network bandwidth to keep the real-time transport of DSR data over either wirelined or wireless channels.
文摘For the convenience of people with disability and for normal people, a demand for intelligent interfaces is ever increasing and therefore related studies are actively being conducted. Recently a study is being conducted to develop an interface through face expression, movement of the body and eye movements, and further more active attempts to use electrical signals(brainwave, electrocardiogram, electromyogram) measured from the human body is also actively being progressed. In addition, the development and the usage of mobile devices and smart devices are promoting these research activities even more. The brainwave is measured by electrical activities between nerve cells in the cerebral cortex using scalp electrodes. The brainwave is mainly used for diagnosis and treatment of diseases such as epilepsy, encephalitis, brain tumors and brain damage. As a result, the brainwave measurement methods and analytical methods were developed. Interface using the brainwave will not go through language or body behavior which is the result of the information processed by the brain but will pass directly to the system providing a brain-computer interface (BCI). This is possible because a variety of the brainwave appears depending on the human’s physical and mental state. Using the brainwave with the intelligent brain-computer interface or combining it with mobile devices and smart devices, regardless of space constraints, the brainwave measurement should be possible.[4,7] In this study, in order to measure the brainwave without spatial constraint, 16 channel compact brainwave measurements system using a high-speed wireless communications were designed. It was designed with a 16 channel to classify the various brainwave patterns that appear and for estimating the location of the nerve cells that triggered the brainwave. And in order to transmit the brainwave data within the channel without loss, a high-speed wireless communication must be possible that can enable a high-speed wireless transmission more sufficient than the Bluetooth, therefore, 802.11 compliant Wi-Fi communication methods were used to transfer the data to the PC. In addition, by using an analog front-end IC having a single-chip configuration with real-time digital filters, the miniaturization of the system was implemented and in order to verify the system Eye-blocking was used to observe the changes in the EEG signal.
基金Supported by Jiangsu Agricultural Science and Technology Independent Innovation Fund[CX(13)3069]~~
文摘[Objective] This study aimed to establish a TaqMan-based real-time PCR assay for detecting transmissible gastroenteritis virus (TGEV). [Method] Primers and a probe were designed according to the conserved sequence of N gene in TGEV genome. After gradient dilution, the recombinant plasmid harboring the N gene was used as a standard for real-time PCR assay to establish the standard curve. [Re- sult] The results showed that the established real-time PCR assay exhibited a good linear relationship within the range of 102-10^10 copies/ul; the correlation coefficient was above 0.99 and the amplification efficiency ranged from 90% to 110%. The de- tection limit of real-time PCR assay for TGEV was 10 copies/μl, suggesting a high sensitivity; there was no cross reaction with other porcine viruses, indicating a good specificity; coefficients of variation within and among batches were lower than 3%, suggesting a good repeatability. The established real-time PCR method could be ap- plied in quantitative analysis and evaluation of the immune efficacy of TGEV vac- cines and detection of TGEV in clinical samples. [Conclusion] The TaqMan-based real-time PCR assay established in this study is highly sensitive and specific, which can provide technical means for the epidemiological survey of TGEV, development of TGEV vaccines and investigation of the pathogenesis of TGE.
基金supported in part by the National Key Research and Development Program of China(2021YFB3900300)in part by the National Natural Science Foundation of China(62201416)+2 种基金in part by the Fundamental Research Funds for the Central Universities(QTZX23070)in part by the Qin Chuang Yuan High-Level Innovative and Entrepreneurial Talents Project(QCYRCXM-2022-314)in part by Singapore Ministry of Education Academic Research Fund Tier 1。
文摘This work presents an optimal design method of antenna aperture illumination for microwave power transmission with an annular collection area.The objective is to maximize the ratio of the power radiated on the annular collection area to the total transmitted power.By formulating the aperture amplitude distribution through a summation of a special set of series,the optimal design problem can be reduced to finding the maximum ratio of two real quadratic forms.Based on the theory of matrices,the solution to the formulated optimization problem is to determine the largest characteristic value and its associated characteristic vector.To meet security requirements,the peak radiation levels outside the receiving area are considered to be extra constraints.A hybrid grey wolf optimizer and Nelder–Mead simplex method is developed to deal with this constrained optimization problem.In order to demonstrate the effectiveness of the proposed method,numerical experiments on continuous apertures are conducted;then,discrete arrays of isotropic elements are employed to validate the correctness of the optimized results.Finally,patch arrays are adopted to further verify the validity of the proposed method.
基金The National Natural Science Foundation of China(No.61201175,61171081)Transformation Program of Science and Technology Achievements of Jiangsu Province(No.BA2010023)
文摘Abts ract A wireless mutl i-hop videot ransmission experiment system is designed and implemented for vehiculra ad-hoc networks VANET and the rt ansm ission control protocol and routing protocol are proposed. This system in tegrates the embedded Linux system witha n ARM kernel and oc ns ists of a S3C6410 main control module a wirel ss local arean etwork WLAN card a LCD screne and so on.In the scenario of a wireless multi-hop video transmission both the H.264 and JPEG are used and their performances such as the compression rate delay and frame loss rate are analyzed in theory andc ompared in the experiment.The system is tested in the real indoor and outdoor environment.The results show that the scheme of the multi-hop video transmission experiment system can be applicable for VANET and multiple scenes and the transmission control protocol and routing protocol proposed can achieve real-time transmission and meet multi-hop requirements.
基金supported by the National Natural Science Foundation of China (under grants 41874048,41790464,41790462).
文摘A rapidly deployable dense seismic monitoring system which is capable of transmitting acquired data in real time and analyzing data automatically is crucial in seismic hazard mitigation after a major earthquake.However,it is rather difficult for current seismic nodal stations to transmit data in real time for an extended period of time,and it usually takes a great amount of time to process the acquired data manually.To monitor earthquakes in real time flexibly,we develop a mobile integrated seismic monitoring system consisting of newly developed nodal units with 4G telemetry and a real-time AI-assisted automatic data processing workflow.The integrated system is convenient for deployment and has been successfully applied in monitoring the aftershocks of the Yangbi M_(S) 6.4 earthquake occurred on May 21,2021 in Yangbi County,Dali,Yunnan in southwest China.The acquired seismic data are transmitted almost in real time through the 4G cellular network,and then processed automat-ically for event detection,positioning,magnitude calculation and source mechanism inversion.From tens of seconds to a couple of minutes at most,the final seismic attributes can be presented remotely to the end users through the integrated system.From May 27 to June 17,the real-time system has detected and located 7905 aftershocks in the Yangbi area before the internal batteries exhausted,far more than the catalog provided by China Earthquake Networks Center using the regional permanent stations.The initial application of this inte-grated real-time monitoring system is promising,and we anticipate the advent of a new era for Real-time Intelligent Array Seismology(RIAS),for better monitoring and understanding the subsurface dynamic pro-cesses caused by Earth's internal forces as well as anthropogenic activities.
基金supported by National Key Technology Research and Development Program of China under Grant No.2015BAH08F01the joint fund of the Ministry of Education of People's Republic of China and China Mobile Communications Corporation under Grant No.MCM20160304
文摘Multi-channel can be used to provide higher transmission ability to the bandwidth-intensive and delay-sensitive real-time streams. However, traditional channel capacity theories and coding schemes are seldom designed for the real-time streams with strict delay constraint, especially in multi-channel context. This paper considers a real-time stream system, where real-time messages with different importance should be transmitted through several packet erasure channels, and be decoded by the receiver within a fixed delay. Based on window erasure channels and i.i.d.(identically and independently distributed) erasure channels, we derive the Multi-channel Real-time Stream Transmission(MRST) capacity models for Symmetric Real-time(SR) streams and Asymmetric Real-time(AR) streams respectively. Moreover, for window erasures, a Maximum Equilibrium Intra-session Code(MEIC) is presented for SR and AR streams, and is shown able to asymptotically achieve the theoretical MRST capacity. For i.i.d. erasures, we propose an Adaptive Maximum Equilibrium Intra-session Code(AMEIC), and then prove AMEIC can closely approach the MRST transmission capacity. Finally, the performances of the proposed codes are verified by simulations.
文摘Accuracy measurement of the Non-soluble Deposit Density (NSDD) on the insulator surface is very important for the transmission line anti-pollution flashover works. A method to measure the NSDD on double sheds porcelain insulator surface based on laser transmission principle is proposed in this paper. Laser unit and luminous intensity sensor are installed between the up and down surface of the double sheds porcelain insulators, two glass tablets are put between the double sheds. The contamination on the glass tablets will influence the luminous intensity that reaches the intensity sensor. The luminous signal is changed to electrical signal, and the insulator’s NSDD could be obtained based on the difference of luminous intensity. The device can be used in online monitoring of the insulator's NSDD condition on the insulator surface.
基金supported by Tehran University of Medical Sciences(No.P/943)
文摘In more than half of infertile men, the cause of their infertility is unknown. Several studies revealed the role of viral infections in male infertility. The aim of the present study was to determine the prevalence of herpes simplex virus-1 (HSV-1) and HSV-2 in semen from asymptomatic infertile male patients, and its association with altered semen parameters. A total of 70 semen samples were collected from infertile men who attended the Research and Clinical Center for Infertility in Yazd, Iran. Semen analysis and diagnostic real-time PCR using specific primers and probes for HSV-1 and HSV-2 DNA were performed. Comparison of semen parameters between virally in- fected and non-infected samples were performed with independent t-test and Mann-Whitney test. Semen analysis showed that infertile men fell into two groups, the male factor group and the unexplained group. HSV-1 and HSV-2 DNA was detected in 16 (22.9%) and 10 (14.3%) of 70 semen samples, respectively. All HSV-positive samples had abnormal semen parameters (the male factor group). Although HSV infection was not associated with sperm motility and morphological defects, it was correlated with lower sperm count in the seminal fluid. The findings suggest that asymptomatic seminal infection of HSV plays an important role in male infertility by adversely af- fecting sperm count.
文摘Objective: To explore the functions and mechanisms of herpes simplex virus type I(HSV-1) while infecting human oral epithelial cells in vitro(being similar to the infection in vivo). Methods:An abundance of HSV-1 strains amplified in Vero cells were used to infect human oral epithelial cells. The culture supernatant was collected to infect Vero cells again. Morphology of HSV-1 was identified by inverted microscope and transmission electron microscope. Nucleic acid of the virus was detected by PCR. Results:The infected human oral epithelial cells didn' t display an obvious cytopathic effect(CPE) under inverted microscope(while Vero cells which were infected by the culture supernatant showed typical(CPE). The virus particles were not observed in the cytoplasm nor in nucleus of human oral epithelial cells, however under transmission electron microscope in the cytoplasm of Vero cells, the nucleic acid of HSV-1 could be detected in infected human oral epithelial cells, by PCR. Conclusion-HSV-1 can successfully infect human oral epithelial cells. This model may provide a useful approach for studying the pathogenesis of herpes virus-associated periodontal disease.
基金This work was financially supported by the National Natural Science Foundation of China(NSFC)(No.61965012)Science and Technology Major Project of Yunnan Province(No.202202AG050004)+5 种基金Technological Innovation Fund Project of Small and Medium-sized Enterprises of Yunnan Province(No.202104AP080068)Yunnan Provincial Natural Science Foundation(Nos.202001AS070008 and 202101AT070126)Yunnan Ten Thousand Talents Plan Young&Elite Talents Project(No.YNWR-QNBJ-2018-295)the Excellent Youth Project of Yunnan Province Applied Basic Research Project(No.2019FI001)Rare and Precious Metal Materials Genome Engineering Project of Yunnan Province(No.202002AB080001)Sichuan Natural Science Foundation(Nos.2022YFH0108 and 2022JDJQ0030).
文摘In-situ observation of the growth and decomposition processes is significantly important for guiding the fabrication of upconversion nanocrystals(UCNCs)with high performance.However,the high crystallization energy and rapid nucleation rate of the corresponding crystals make real-time observation still a huge challenge.Herein,the in-situ nucleation-growth-degradation processes of the UCNCs are investigated by employing tailored metastable intermediates,which possess a slowing-down nucleation rate under electron beam irradiation.The non-classical nucleation processes of the UCNCs,containing the coalescence of clusters and the subsequent crystallization,are demonstrated.Moreover,the Ostwald ripening and oriented attachment processes,which determine the particle size and morphology,are unambiguously recorded.Furthermore,the degrading process of the UCNCs is observed to be triggered by surface defects.Our work provides an insight into the real-time evolution dynamics of the UCNCs,which further sheds light on the fabrication of nano-sized up-conversion phosphors.