Effects of the simulated acid rain（AR） and ultraviolet-B（UV-B, 280-320 nm） radiation with a single or two ways simultaneously （AR ＋ UV-B） on the antioxidant enzyme and photosynthesis of the rape seedlings were ...Effects of the simulated acid rain（AR） and ultraviolet-B（UV-B, 280-320 nm） radiation with a single or two ways simultaneously （AR ＋ UV-B） on the antioxidant enzyme and photosynthesis of the rape seedlings were investigated by the hydroponic culture. The results of static experiment indicated that the tolerance of rape seedling to single stress（AR or UV-B） is stronger than that to dual stresses（AR ＋ UV-B）. Furthermore, the dual stresses had additive effect on catalase activity, and a synergistic effect on MDA content, net photosynthesis rate, water use efficiency as well as intercellular CO2 concentration. Meanwhile, it has an independent effect on chlorophyll content, stomatal conductance, and transpiration rate as well as membrane permeability. During 64 h restoration course, the dynamic change in the curves of physiological and biochemical indices were not identical, and none of them show a simple linear variation. According to the static and dynamic experiments, it was found that a responsive sequence of catalase activity, membrane permeability, MDA content and photosynthetic characteristics to the above-mentioned stresses was as follows： AR ＋ UV-B 〉 UV-B 〉 AR.展开更多
Seed germination of plants with various acid-resis tance display different responses to acid rain.To understand the reason why such differences occur,the effects of sim ulated acid rain(pH 2.5-5.0)on the activities of...Seed germination of plants with various acid-resis tance display different responses to acid rain.To understand the reason why such differences occur,the effects of sim ulated acid rain(pH 2.5-5.0)on the activities of peroxidase(POD)and catalase(CAT)during sced ger-mination of rice(O.sativa),wheat(T.aestivurm),and rape(B.chinensis var.oleifera)were investigated.R cesults indi-cated that the maximum change in activities of CAT and POD by acid rain treatment with different acidity and time in relation to the referent treatment without acid rain,was in the order:rice(28.8%,31.7%)<wheat(34.7%,48.3%)<rape(79.3%,50.0%).The pH level for which the treatment with acid rain did not cause signif-cant difference(p<0.05)was in the order:rice(3.5)>wheat(4.0)>rape(5.0).Moreover,the change in activity of POD was higher than that of CAT,which showed that POD was more sensitive to acid rain stress than CAT.The difference in the ability of POD and CAT in removing free radicals was one reason why the germina-tion indexes of these three species behaved differently.展开更多
文摘Effects of the simulated acid rain（AR） and ultraviolet-B（UV-B, 280-320 nm） radiation with a single or two ways simultaneously （AR ＋ UV-B） on the antioxidant enzyme and photosynthesis of the rape seedlings were investigated by the hydroponic culture. The results of static experiment indicated that the tolerance of rape seedling to single stress（AR or UV-B） is stronger than that to dual stresses（AR ＋ UV-B）. Furthermore, the dual stresses had additive effect on catalase activity, and a synergistic effect on MDA content, net photosynthesis rate, water use efficiency as well as intercellular CO2 concentration. Meanwhile, it has an independent effect on chlorophyll content, stomatal conductance, and transpiration rate as well as membrane permeability. During 64 h restoration course, the dynamic change in the curves of physiological and biochemical indices were not identical, and none of them show a simple linear variation. According to the static and dynamic experiments, it was found that a responsive sequence of catalase activity, membrane permeability, MDA content and photosynthetic characteristics to the above-mentioned stresses was as follows： AR ＋ UV-B 〉 UV-B 〉 AR.
基金This work was supported by the Foundation of State Planning Committee(No.GJX01100626)the Foundation of Science and Technology Committee of Jiangsu Province(Grant No.BG2001045)the Foundation from Key Laboratory of Industrial Biotechnology of Ministry of Education,Jiangnan University of China.
文摘Seed germination of plants with various acid-resis tance display different responses to acid rain.To understand the reason why such differences occur,the effects of sim ulated acid rain(pH 2.5-5.0)on the activities of peroxidase(POD)and catalase(CAT)during sced ger-mination of rice(O.sativa),wheat(T.aestivurm),and rape(B.chinensis var.oleifera)were investigated.R cesults indi-cated that the maximum change in activities of CAT and POD by acid rain treatment with different acidity and time in relation to the referent treatment without acid rain,was in the order:rice(28.8%,31.7%)<wheat(34.7%,48.3%)<rape(79.3%,50.0%).The pH level for which the treatment with acid rain did not cause signif-cant difference(p<0.05)was in the order:rice(3.5)>wheat(4.0)>rape(5.0).Moreover,the change in activity of POD was higher than that of CAT,which showed that POD was more sensitive to acid rain stress than CAT.The difference in the ability of POD and CAT in removing free radicals was one reason why the germina-tion indexes of these three species behaved differently.
