A Non-linear Non-ideal Model of Simulated Moving Bed Chromatography for Chiral Separations

A non-linear non-ideal model,taking into account non-linear competitive isotherms,axial disperison,film mass transfer,intraparticle diffusion,and port periodic switching,was developed to simulate the dynamics of simulated moving bed chromatography(SMBC),The model equations were solved by a new efficient numerical technique of orthogonal collocation on finite elements with periodical movement of conceantration vector,The simulated SMBC performance is in accordance with the experimental results reported in the literature for separation of 1,1''''''''-bi-2-naphtol enantiomers using SMBC,This model is useful for design,operation ,optimization and scale-up of non-linear SMBC for chiral separations with significant non-ideal effects,especially for high solute concentration and small intraparticle diffusion coefficient or large chiral stationary phase particle.

Numerical Simulation of Asynchronous Simulated Moving Bed Chromatography

Asynchronous simulated moving bed chromatography (ASMBC), known also as the 'VARICOL' process, is more efficient and flexible than the well-known and traditional simulated moving bed chromatography (SMBC). A detailed model of ASMBC, taking account of non-linear competitive isotherms, mass transfer parameters, and complex port switching schedule parameters, was developed to simulate the complex dynamics of ASMBC.The simulated performance is in close agreement with the experimental data of chiral separation reported in the literature. The simulation results show that ASMBC can achieve the performance similar to SMBC with fewer columns and can achieve better performance than SMBC with the same total column number. All design and operation parameters can be chosen correctly by numerical simulation. This detailed ASMBC model and the numerical technique are useful for design, operation, optimization and scale-up of ASMBC.

Purification of L-Lysine in Simulated Moving Bed and Fixed-Bed Chromatography

L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum （ATCC 21799） strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed （SMB） chromatography. The separation conditions including pH, eluent concentration and Lys+ and Lys2+ adsorption isotherms were studied in batch adsorption. The column capacity, eluent flow rate and eluent concentration have been studied in fixed-bed chromatography. Maximum purification rate of lysine was obtained as 0.066 （g/（g·h）） （per gram resin and per hour） at an eluent flow rate of 10 （mL/min） in fixed-bed chromatography. The results obtained from SMB were 0.11 （g/（g·h）） for L-lysine purification rate and 96% for L-lysine recovery.

Mathematical modeling of salt-gradient ion-exchange simulated moving bed chromatography for protein separations

The salt-gradient operation mode used in ion-exchange simulated moving bed chromatography (SMBC) can improve the efficiency of protein separations. A detailed model that takes into account any kind of adsorption/ion-exchange equilibrium, salt gradient, size exclusion, mass transfer resistance, and port periodic switching mechanism, was developed to simulate the complex dynamics. The model predictions were verified by the experimental data on upward and downward gradients for protein separations reported in the literature. All design and operating parameters (number, configuration, length and diameter of columns, particle size, switching period, flow rates of feed, raffinate, desorbent and extract, protein concentrations in feed, different salt concentrations in desorbent and feed) can be chosen correctly by numerical simulation. This model can facilitate the design, operation, optimization, control and scale-up of salt-gradient ion-exchange SMBC for protein separations.

Separation of phosphatidylcholine from soybean phospholipids by simulated moving bed

A simulated moving bed (SMB), equipped with eight silica-gel columns, was used to separate phosphatidylcholine (PC) from soybean phospholipids. The effects of flow rate in Sections 2 (Q2) and 3 (Q3), switching time, feed flow rate and feed concentration on the operating performance parameters: purity, recovery, productivity and desorbent consumption were studied. Operating conditions leading to more than 90% purity in both outlet streams have been identified, together with those achieving optimal performance. Regions leading to complete separation are observed and explained theoretically. As the mass-transfer effect was not considered, the triangle theory only gives initial guesses for the optimal operating conditions.

SMB色谱分离过程的建模与优化

基于物料平衡理论建立了 SMB色谱分离过程的理想数学模型。以提高产品纯度和收率为目标 ,依据 Massimo三角形理论 ,研究了主要操作参数对 SMB性能的影响。针对具体的分离对象进行了仿真和操作条件寻优 ,并以此指导实际分离实验 ,得到满意的结果。说明了简化的模型在操作优化。

SMB技术分离糖与糖醇的应用

本文简述了模拟移动床技术的原理,并着重介绍了其在分高糖类和糖醇类等食品添加剂混合物中的应用,运用该技术分离分子结构相近的物质有独特的分离效果。

SMB两组份色谱分离过程建模与优化

基于TMB建模方法建立了二组份SMB色谱分离过程的理想数学模型。以提高产品纯度和收率为目标 ,依据Massimo三角形理论 ,研究了主要操作参数对SMB性能的影响。针对具体的分离对象进行了操作条件寻优仿真 ,并以此指导实际分离实验 。

SMB色谱分离过程的Matlab仿真

基于考虑因素全面的综合速率模型 ,通过对柱向和吸附颗粒径向模型采用有限元法和正交配点法进行离散化 ,利用MatlabODE求解器对离散化得到的常微分方程进行数值求解 ,在此基础上设计了仿真软件。以具体仿真实例 ,验证了所用方法的可行和有效性。

模拟移动床色谱分离技术研究进展

模拟移动床色谱(Simulated moving bed chromatography,SMBC)是一种新型分离手段,具有分离效果好、投资成本少以及适合连续性大规模生产等特点,近些年来备受关注,研究热度居高不下,在食品、化工和制药等行业均有广泛应用。SMBC由模拟移动床技术与色谱分离技术相结合产生,通过回顾色谱分离技术的起源,介绍模拟移动床色谱分离技术的原理及应用领域,列举模拟移动床色谱在高效分离淀粉糖、低聚糖、糖醇及有机酸等混合物的实际应用场景,为该技术的工业应用提供参考。

SMB色谱分离过程简化模型辨识

在线性等温线条件下针对葡萄糖和果糖两组分SMB色谱分离过程,依据机理模型设计了恰当的仿真实验,基于MIMO-ARX模型类对其进行了简化模型辨识。根据模型验证结果,分析了不同样本数据和模型结构参数对模型精度的影响。获得的简化模型对机理模型的动态行为具有较好的逼近能力．对基于模型的控制器设计具有一定的指导意义。

SMB色谱分离过程的动态矩阵控制

针对SMB色谱分离过程的工作原理,分析了现有系统开环不加控制时过程运行的缺陷,在机理模型的基础上通过辨识得到了对象局部简化模型。根据此模型设计了多变量动态矩阵控制器,实现了过程的预测控制。通过仿真实验分别比较了系统在不加控制和DMC控制时在轨迹跟踪和扰动抑制方面的性能,结果表明采用DMC控制算法的SMB过程,比不加控制时的系统有良好的性能和优越性,从而为系统的实际运行提供理论基础。

传统脱盐方法及新型DSSMB脱盐技术

脱盐是将溶液中"化学盐"脱除的精制提纯过程。简述了传统脱盐技术中离子交换法、膜分离法、电渗析法的技术特点和应用现状。着重介绍模拟移动床DSSMB色谱脱盐新技术、设备及其特点。DSSMB脱盐技术具有无需添加酸碱、清洁生产、脱盐效率显著、自动化程度高、适用范围广、同时实现脱盐脱色及多组分分离等优点,尤其适用于复杂物料的分离,极具应用前景和推广价值。

Continuous size fractionation of magnetic nanoparticles by using simulated moving bed chromatography

The size fractionation of magnetic nanoparticles is a technical problem,which until today can only be solved with great effort.Nevertheless,there is an important demand for nanoparticles with sharp size distributions,for example for medical technology or sensor technology.Using magnetic chromatography,we show a promising method for fractionation of magnetic nanoparticles with respect to their size and/or magnetic properties.This was achieved by passing magnetic nanoparticles through a packed bed of fine steel spheres with which they interact magnetically because single domain ferro-/ferrimagnetic nanoparticles show a spontaneous magnetization.Since the strength of this interaction is related to particle size,the principle is suitable for size fractionation.This concept was transferred into a continuous process in this work using a so-called simulated moving bed chromatography.Applying a suspension of magnetic nanoparticles within a size range from 20 to 120 nm,the process showed a separation sharpness of up to 0.52 with recovery rates of 100%.The continuous feed stream of magnetic nanoparticles could be fractionated with a space-time-yield of up to 5 mg/(L∙min).Due to the easy scalability of continuous chromatography,the process is a promising approach for the efficient fractionation of industrially relevant amounts of magnetic nanoparticles.

Simulated moving bed separation of tocopherol homologues:simulation and experiments

Chromatograms of tocopherol homologues were obtained by a column of analytical size (inner diameter (ID) 0.46 cm cm×10 cm) packed with silica gel. Adsorption isotherms and film mass-transfer coefficient were estimated from the chroma-tograms by using a general rate model, which considers axial dispersion, external mass-transfer and intraparticle diffusion. Based on the obtained isotherms and mass-transfer coefficient, the separation process of tocopherol homologues on simulated moving bed (SMB) was simulated using the same model. According to the simulated results, a mixture of α-, γ-, δ-tocopherols and other impurities was separated on an SMB equipment. The SMB equipment was composed of 8 columns of ID 2 cm×10 cm, with 2 columns in each section. The solid phase was silica gel, and the mobile phase was n-hexane/2-propanol (99/1 by volume). γ-and δ-tocopherols of purity greater than 98% were obtained with recovery greater than 98%. The effects of operating conditions (flow rates and switching time) on the performance of SMB were studied by both simulation and experiments. It was found that all the simulation results were quite close to the experimental results. We conclude that process development and optimization of operating conditions of SMB by simulation are feasible.

模拟移动床色谱技术及其应用

详细地介绍了一种现代化分离技术模拟移动床色谱技术的发展历史及其工作原理,同时介绍了模拟移动床色谱在石油化工、糖醇分离、手性化合物及其他方面的主要应用。

生产银杏黄酮的模拟移动床色谱工艺

Separation of Ginkgo flavonoids using simulated moving bed chromatography to replace the batch chromatography is discussed.The product of higher purity is obtained,and in this process,the yield of product increased and cost decreased.Furthermore,this process is very clean.

银杏内酯B的纯化

报道了具有生产规模的银杏内酯B(GB)纯化工艺。该工艺包括萃取、模拟移动床、结晶3个步骤。其核心是用模拟移动床色谱对银杏内酯B进行规模化精细分离。所得产品纯度w(GB)≥90%,收率≥70%,生产能力20g/h。生产1g银杏内酯B净消耗乙酸乙酯29L,乙醇50mL,甲醇40mL。产物用高效液相色谱、液质联用及核磁共振做了鉴定。

模拟移动床色谱分离替考拉宁条件研究

探讨了用模拟移动床色谱分离提纯替考拉宁的可行性 ,且优化出最佳分离条件为 ts=1 0min,υF=0 .0 8m L/min,υp=1 .5 m L/min,υD=0 .8m L/min,CF=8mg/m L。