Winter jujube(Ziziphus jujuba'Dongzao')is greatly appreciated by consumers for its excellent quality,but brand infringement frequently occurs in the market.Here,we first determined a total of 38 elements in 16...Winter jujube(Ziziphus jujuba'Dongzao')is greatly appreciated by consumers for its excellent quality,but brand infringement frequently occurs in the market.Here,we first determined a total of 38 elements in 167 winter jujube samples from the main winter jujube producing areas of China by inductively coupled plasma mass spectrometer(ICP-MS).As a result,16 elements(Mg,K,Mn,Cu,Zn,Mo,Ba,Be,As,Se,Cd,Sb,Ce,Er,Tl,and Pb)exhibited significant differences in samples from different producing areas.Supervised linear discriminant analysis(LDA)and orthogonal projection to latent structures discriminant analysis(OPLS-DA)showed better performance in identifying the origin of samples than unsupervised principal component analysis(PCA).LDA and OPLS-DA had a mean identification accuracy of 87.84 and 94.64%in the testing set,respectively.By using the multilayer perceptron(MLP)and C5.0,the prediction accuracy of the models could reach 96.36 and 91.06%,respectively.Based on the above four chemometric methods,Cd,Tl,Mo and Se were selected as the main variables and principal markers for the origin identification of winter jujube.Overall,this study demonstrates that it is practical and precise to identify the origin of winter jujube through multi-element fingerprint analysis with chemometrics,and may also provide reference for establishing the origin traceability system of other fruits.展开更多
建立一种采用液相色谱法同时测定亚麻荠植株中8种酚酸和黄酮化合物含量的方法,并探讨亚麻荠植株醇提物的体外抗氧化活性。试验确定采用体积分数75%乙醇回流提取样品,选用Angilent Eclipse XDB plus C_(18)柱为色谱柱。结果表明:8种化合...建立一种采用液相色谱法同时测定亚麻荠植株中8种酚酸和黄酮化合物含量的方法,并探讨亚麻荠植株醇提物的体外抗氧化活性。试验确定采用体积分数75%乙醇回流提取样品,选用Angilent Eclipse XDB plus C_(18)柱为色谱柱。结果表明:8种化合物的标准曲线回归方程线性关系良好,相关系数均大于0.999;8种化合物的加标回收率为95.26%~102.61%,建立的方法可以对不同产地的亚麻荠植株样品进行区分。抗氧化试验表明,亚麻荠植株醇提取物对羟基自由基与DPPH自由基具有较好的清除能力。展开更多
目的:建立一种分析葆宫止血颗粒中13个成分的高效液相色谱法,并结合化学计量学及灰色关联度分析对葆宫止血颗粒进行质量评价。方法:采用50%甲醇超声提取样品,CAPCELL PAK C_(18)色谱柱,乙腈-0.2%磷酸水溶液为流动相梯度洗脱,同时测定葆...目的:建立一种分析葆宫止血颗粒中13个成分的高效液相色谱法,并结合化学计量学及灰色关联度分析对葆宫止血颗粒进行质量评价。方法:采用50%甲醇超声提取样品,CAPCELL PAK C_(18)色谱柱,乙腈-0.2%磷酸水溶液为流动相梯度洗脱,同时测定葆宫止血颗粒中梓醇、地黄苷D、地黄苷A、益母草苷、三七皂苷R_(1)、人参皂苷Rg_(1)、人参皂苷Rb_(1)、柴胡皂苷a、柴胡皂苷d、芍药内酯苷、芍药苷、苯甲酰芍药苷和鹤草酚的含量。采用化学计量学挖掘影响葆宫止血颗粒产品质量的差异标志物,结合灰色关联度法计算各指标的关联度并对13批葆宫止血颗粒的质量进行排序。结果:13个成分的线性范围分别为2.68~134.00、1.64~82.00、0.88~44.00、0.57~28.50、2.35~117.50、11.93~596.50、9.71~485.50、2.05~102.50、1.86~93.00、9.62~481.00、19.05~952.50、1.78~89.00、0.66~33.00µg·mL^(–1)(r>0.999),3个质量浓度水平加样回收率为96.78%~100.12%(RSD<2.0%)。化学计量学分析显示,芍药苷、人参皂苷Rg_(1)、芍药内酯苷、人参皂苷Rb_(1)、梓醇、柴胡皂苷a和三七皂苷R_(1)是影响葆宫止血颗粒产品质量的主要标志物。灰色关联度分析相对关联度为0.3446~0.5742。结论:该方法揭示了影响葆宫止血颗粒质量的主要潜在标志物,综合评价了葆宫止血颗粒的质量,可为其内在整体质量控制提供参考。展开更多
基金This work was supported by the Scientific Research Foundation for High Level Talents of Qingdao Agricultural University,China(665-1120015)the National Program for Quality and Safety Risk Assessment of Agricultural Products of China(GJFP2019011)the National Natural Science Foundation of China(42207017).
文摘Winter jujube(Ziziphus jujuba'Dongzao')is greatly appreciated by consumers for its excellent quality,but brand infringement frequently occurs in the market.Here,we first determined a total of 38 elements in 167 winter jujube samples from the main winter jujube producing areas of China by inductively coupled plasma mass spectrometer(ICP-MS).As a result,16 elements(Mg,K,Mn,Cu,Zn,Mo,Ba,Be,As,Se,Cd,Sb,Ce,Er,Tl,and Pb)exhibited significant differences in samples from different producing areas.Supervised linear discriminant analysis(LDA)and orthogonal projection to latent structures discriminant analysis(OPLS-DA)showed better performance in identifying the origin of samples than unsupervised principal component analysis(PCA).LDA and OPLS-DA had a mean identification accuracy of 87.84 and 94.64%in the testing set,respectively.By using the multilayer perceptron(MLP)and C5.0,the prediction accuracy of the models could reach 96.36 and 91.06%,respectively.Based on the above four chemometric methods,Cd,Tl,Mo and Se were selected as the main variables and principal markers for the origin identification of winter jujube.Overall,this study demonstrates that it is practical and precise to identify the origin of winter jujube through multi-element fingerprint analysis with chemometrics,and may also provide reference for establishing the origin traceability system of other fruits.
文摘建立一种采用液相色谱法同时测定亚麻荠植株中8种酚酸和黄酮化合物含量的方法,并探讨亚麻荠植株醇提物的体外抗氧化活性。试验确定采用体积分数75%乙醇回流提取样品,选用Angilent Eclipse XDB plus C_(18)柱为色谱柱。结果表明:8种化合物的标准曲线回归方程线性关系良好,相关系数均大于0.999;8种化合物的加标回收率为95.26%~102.61%,建立的方法可以对不同产地的亚麻荠植株样品进行区分。抗氧化试验表明,亚麻荠植株醇提取物对羟基自由基与DPPH自由基具有较好的清除能力。
文摘目的:建立一种分析葆宫止血颗粒中13个成分的高效液相色谱法,并结合化学计量学及灰色关联度分析对葆宫止血颗粒进行质量评价。方法:采用50%甲醇超声提取样品,CAPCELL PAK C_(18)色谱柱,乙腈-0.2%磷酸水溶液为流动相梯度洗脱,同时测定葆宫止血颗粒中梓醇、地黄苷D、地黄苷A、益母草苷、三七皂苷R_(1)、人参皂苷Rg_(1)、人参皂苷Rb_(1)、柴胡皂苷a、柴胡皂苷d、芍药内酯苷、芍药苷、苯甲酰芍药苷和鹤草酚的含量。采用化学计量学挖掘影响葆宫止血颗粒产品质量的差异标志物,结合灰色关联度法计算各指标的关联度并对13批葆宫止血颗粒的质量进行排序。结果:13个成分的线性范围分别为2.68~134.00、1.64~82.00、0.88~44.00、0.57~28.50、2.35~117.50、11.93~596.50、9.71~485.50、2.05~102.50、1.86~93.00、9.62~481.00、19.05~952.50、1.78~89.00、0.66~33.00µg·mL^(–1)(r>0.999),3个质量浓度水平加样回收率为96.78%~100.12%(RSD<2.0%)。化学计量学分析显示,芍药苷、人参皂苷Rg_(1)、芍药内酯苷、人参皂苷Rb_(1)、梓醇、柴胡皂苷a和三七皂苷R_(1)是影响葆宫止血颗粒产品质量的主要标志物。灰色关联度分析相对关联度为0.3446~0.5742。结论:该方法揭示了影响葆宫止血颗粒质量的主要潜在标志物,综合评价了葆宫止血颗粒的质量,可为其内在整体质量控制提供参考。
