Advances in single-cell sequencing technology in microbiome research

With the rapid development of histological techniques and the widespread applica-tion of single-cell sequencing in eukaryotes,researchers desire to explore individual microbial.genotypes and functional expression,which deepens our understanding of microorganisms.In this review,the history of the development of microbial detection technologies was revealed and the difficulties in the application of single-cell sequencing in microorganisms were dissected as well.Moreover,the characteristics of the currently emerging microbial single-cell sequencing(Microbe-seq)technology were summarized,and the prospects of the application of Microbe-seq in microorganisms were distilled based on the current development status.Despite its mature development,the Microbe-seq technology was still in the optimization stage.A retrospective study was conducted,aiming to promote the widespread application of single-cell sequencing in microorganisms and facilitate further improvement in the technol-ogy.

Recent progress in co-detection of single-cell transcripts and proteins

Cellular heterogeneity is a universal property of living systems,and the interrogation of single cells facilitates in-depth understanding of distinct cellular states and functions in various biological processes.Co-analysis of transcripts and proteins from the same single cells opens the way to decipher complex RNA regulatory frameworks and phenotypes,facilitating the understanding of cellular fate and function regulations,discovery of novel cell types,and construction of a high-resolution cell atlas.Herein,we review the state-of-art advances in the development of methodologies for co-analysis of single-cell transcripts and proteins.First,imaging-based methods are summarized with particular emphasis on optical and mass spectrometry imaging.Next,sequencing-based approaches for high-throughput and sensitive co-analysis of single-cell transcripts and proteins are described,including droplet-,microwell-,and split-pool-based platforms.Subsequently,combined methods with more flexibility and universality are discussed.These methods commonly employ different strategies or reactions to convert transcripts and proteins of single cells into distinct signals simultaneously,which can be detected by different instruments or platforms.Lastly,some perspectives on the future challenges and development trends in this field are presented.

单细胞尺度评价造血干细胞移植术的研究进展

造血干细胞移植术(HSCT)是救治血液系统辐射损伤、恶性血液病和部分重型良性血液病的有效手段之一,但供者移植物的构成以及造血干细胞在受者体内的归巢、植入和分化等关键生物学行为尚需深入解析。近年来,随着单细胞技术的发展,研究人员绘制了造血系统的单细胞图谱。除此之外,单细胞RNA测序(scRNA-seq)已开始用于追踪移植后造血系统的动态变化,为优化HSCT策略及改善移植预后提供了帮助。基于此,本文对单细胞尺度评价HSCT的研究进展及相关领域今后值得关注的方向作一综述。