Study on the Treatment of Acid Mine Drainage Containing Fe^(2+) and Mn^(2+) Using Modified Spontaneous Combustion Gangue

The high concentrations of Fe^(2+) and Mn^(2+) in acid mine drainage make it difficult and expensive to treat.It is urgent that we find a cheap and efficient adsorption material to treat Fe^(2+) and Mn^(2+).As a solid waste in mining areas,coal gangue occupies a large area and pollutes the surrounding environment during the stacking process.Developing a method of resource utilization is thus a research hotspot.In this study,we modified spontaneous combustion gangue using NaOH,NaCl,and HCl by chemically modifying the minerals.We determined the optimal conditions for treating Fe^(2+) and Mn^(2+) in acid mine drainage with spontaneous combustion gangue and modified coal gangue using the single factor test method.Based on results of the static test,two dynamic test columns,column No.1(spontaneous combustion gangue)and column No.2(NaOH modified spontaneous combustion gangue),were constructed,and the repair effects of acid mine drainage were compared and analyzed using dynamic experiments.The results show that overall,NaOH modified spontaneous combustion gangue is the most efficient at removing the Fe^(2+) and Mn^(2+) in acid mine drainage.The optimal conditions for NaOH modification are an NaOH concentration of 3 mol/L,a liquid to solid ratio of 2 L/kg,and a modification time of 8 h.The overall efficiency of column No.2 at removing Fe^(2+) and Mn^(2+) from acid mine drainage is better than that of column No.1.Among them,the average removal efficiency of Fe^(2+)and Mn^(2+) from acid mine drainage in column No.2 were 97.73%and 44.82%,respectively.The above results show that NaOH modified spontaneous combustion gangue is a good adsorbent,which has application potential in wastewater remediation,as it can achieve the purpose of“treating dust with waste”.

Establishment and Optimization of ISSR Reaction System for Elymus sibiricus L.

[ Objective ] This study aimed to establish and optimize the ISSR-PCR reaction system and amplification process for Elymus sibiricus L. , to provide sci- entific basis for exploring the genetic diversity of E. sibiricus germplasm resources. [ Method] Orthogonal design and single factor test were applied to establish the ISSR-PCR reaction system of E. sibiricus, optimize the influencing factors including Taq DNA polymerase, DNA template concentration, Mg2 ＋ , dNTP, primer concentration, and screen the annealing temperature, number of cycles and extension time. [ Result ] The optimal reaction system for ISSR analysis contains 0.2 mmol/L dNTPs, 0.2 μmol/L ISSR primers, 1.5 U of Taq DNA polymerase, 2.5 μl of 10 × PCR Buffer, 1.5 mmol/L MgC12 and 40 ng of template DNA in 25 μl total volume; the amplification was conducted with 35 cycles and extension time of 90 s. [ Conclusion] ISSR-PCR reaction system for E. sibiricus was established and optimized, and then verified using two E. sibiricus germplasrns, demonstrating that the ISSR-PCR reaction system is stable and can be used for the genetic analysis of E. sibiricus.