Comprehensive integration of single-cell transcriptomic data illuminates the regulatory network architecture of plant cell fate specification

Plant morphogenesis relies on precise gene expression programs at the proper time and position which is orchestrated by transcription factors(TFs)in intricate regulatory networks in a cell-type specific manner.Here we introduced a comprehensive single-cell transcriptomic atlas of Arabidopsis seedlings.This atlas is the result of meticulous integration of 63 previously published scRNA-seq datasets,addressing batch effects and conserving biological variance.This integration spans a broad spectrum of tissues,including both below-and above-ground parts.Utilizing a rigorous approach for cell type annotation,we identified 47 distinct cell types or states,largely expanding our current view of plant cell compositions.We systematically constructed cell-type specific gene regulatory networks and uncovered key regulators that act in a coordinated manner to control cell-type specific gene expression.Taken together,our study not only offers extensive plant cell atlas exploration that serves as a valuable resource,but also provides molecular insights into gene-regulatory programs that varies from different cell types.

A new integration algorithm for finite deformation of thermo-elasto-viscoplastic single crystals

An algorithm for integrating the constitutive equations in thermal framework is presented, in which the plastic deformation gradient is chosen as the integration variable. Compared with the classic algorithm, a key feature of this new approach is that it can describe the finite deformation of crystals under thermal conditions. The obtained plastic deformation gradient contains not only plastic defor- mation but also thermal effects. The governing equation for the plastic deformation gradient is obtained based on ther- mal multiplicative decomposition of the total deformation gradient. An implicit method is used to integrate this evo- lution equation to ensure stability. Single crystal 1 100 aluminum is investigated to demonstrate practical applications of the model. The effects of anisotropic properties, time step, strain rate and temperature are calculated using this integration model.

Heavy ion-induced single event upset sensitivity evaluation of 3D integrated static random access memory

Heavy ion-induced single event upsets(SEUs)of static random access memory(SRAM), integrated with three-dimensional integrated circuit technology, are evaluated using a Monte Carlo simulation method based on the Geant4 simulation toolkit. The SEU cross sections and multiple cell upset(MCU) susceptibility of 3D SRAM are explored using different types and energies of heavy ions.In the simulations, the sensitivities of different dies of 3D SRAM show noticeable discrepancies for low linear energy transfers(LETs). The average percentage of MCUs of 3D SRAM increases from 17.2 to 32.95%, followed by the energy of ^(209)Bi decreasing from 71.77 to 38.28 MeV/u. For a specific LET, the percentage of MCUs presents a notable difference between the face-to-face and back-toface structures. In the back-to-face structure, the percentage of MCUs increases with a deeper die, compared with the face-to-face structure. The simulation method and process are verified by comparing the SEU cross sections of planar SRAM with experimental data. The upset cross sections of the planar process and 3D integrated SRAM are analyzed. The results demonstrate that the 3D SRAM sensitivity is not greater than that of the planar SRAM. The 3D process technology has the potential to be applied to the aerospace and military fields.

Influence of Tilted Angle on Effective Linear Energy Transfer in Single Event Effect Tests for Integrated Circuits at 130 nm Technology Node

A heavy-ion irradiation experiment is studied in digital storage cells with different design approaches in 130？nm CMOS bulk Si and silicon-on-insulator （SOI） technologies. The effectiveness of linear energy transfer （LET） with a tilted ion beam at the 130？nm technology node is obtained. Tests of tilted angles θ=0 ° , 30 ° and 60 ° with respect to the normal direction are performed under heavy-ion Kr with certain power whose LET is about 40？MeVcm 2 /mg at normal incidence. Error numbers in D flip-flop chains are used to determine their upset sensitivity at different incidence angles. It is indicated that the effective LETs for SOI and bulk Si are not exactly in inverse proportion to cosθ , furthermore the effective LET for SOI is more closely in inverse proportion to cosθ compared to bulk Si, which are also the well known behavior. It is interesting that, if we design the sample in the dual interlocked storage cell approach, the effective LET in bulk Si will look like inversely proportional to cosθ very well, which is also specifically explained.

Design and dynamic analysis of single-axis integrated inertia measurement device

The structure and measurement theory of a single-axis integrated inertia measurement device are discussed in this paper.The acceleration and angle velocity can be detected by the proposed sensor at the same time.The ki- netic model of the device is also established.In addition,the signal generation of the single-axis integrated inertia measurement device is analyzed and simulated.The results of the model are consistent with simulation result.

The analysis of the integral gated mode single photon detector

This paper critically analyses and simulates the circuit configuration of the integral gated mode single photon detector which is proposed for eliminating the transient spikes problem of conventional gated mode single photon detector. The relationship between the values of the circuit elements and the effect of transient spikes cancellation has been obtained. With particular emphasis, the bias voltage of the avalanche photodiode and the output signal voltage of the integrator have been calculated. The obtained analysis results indicate that the output signal voltage of the integrator only relates to the total quantity of electricity of the avalanche charges by choosing the correct values of the circuit elements and integral time interval. These results can be used to optimize the performance of single photon detectors and provide guides for the design of single photon detectors.

New Numerical Integration Formulations for Ordinary Differential Equations

An entirely new framework is established for developing various single- and multi-step formulations for the numerical integration of ordinary differential equations. Besides polynomials, unconventional base-functions with trigonometric and exponential terms satisfying different conditions are employed to generate a number of formulations. Performances of the new schemes are tested against well-known numerical integrators for selected test cases with quite satisfactory results. Convergence and stability issues of the new formulations are not addressed as the treatment of these aspects requires a separate work. The general approach introduced herein opens a wide vista for producing virtually unlimited number of formulations.

The Blue Stragglers and the Integrated Spectral Energy Distribution of Single Stellar Populations

Blue stragglers are a common observational fact for the Galactic clusters. Single Stellar Populations (SSPs) are basic to the studies of galaxy structre and evolution. SSPs are mainly based either on the observation of the integrated properties of star clusters, or on the theoretical understandings of single star evolution. Both of the two ways of making SSPs suffer from either observational uncertainties concerning field contaminations or lack of good models for close binary systems. Based on the photometry of the classical open cluster M67 and the thorough membership survey, we made a color-magnitude diagram (CMD) of high membership stars for the cluster. We will show that by including the contributions of the bright blue stragglers that is common to open clusters, the integrated properties of the clusters are quite different from tranditional SSP models. We further conclude that these blue light contributors are very important to SSP models, and may cast new lights on its applications in the studies of galaxies.

Single Layer Centrifugation with Androcoll-E^(TM) improved progressive motility and percentage of live spermatozoa with intact acrosome of chilled stallion semen but did not have an effect on DNA integrity

A significant number of stallions produce low quality ejaculates with high sensibility to chilling. Single Layer Centrifugation (SLC) with Andro-coll-ETM has been presented as an efficient method of selecting good quality spermatozoa. In the current study, changes in sperm quality (motility, viability, acrosome integrity and DNA damage) occurring during storage at 5?C for a maximum of 72 h, were investigated. For that, one ejaculate from 12 stallions was split in two aliquots: control and SLC-selected. Both aliquots were chilled and stored at 5?C and spermatozoa were evaluated for motility, viability and acrosome integrity at 24, 48 and 72 h post collection. DNA damage was evaluated at 48 h post collection using the comet assay. In the SLC-selected aliquots, there was a significant improvement in terms of progressive motility (0 h: P = 0.005;24 h: P 0.05). SLC with Androcoll-ETM improved semen quality prolonging sperm longevity of chilled semen (P = 0.012). This positive effect was more evident in ejaculates most sensitive to chilling that had a sharp decrease in motility in the first 24 h of refrigeration and for all ejaculates at 72 h post-chilling. Therefore, this method reveals to be a useful technique for selecting spermatozoa and maintain sperm quality during storage.

Building Integrated Photovoltaic Systems for Single Family Dwellings: Innovation Concepts

Growing consumer interest in distributed Building Integrated Photovoltaic (BIPV) Systems and industry competition to reduce installation costs are stimulating the development of deploying these materials to the residential sector of the building industry. This emerging market continues to attract the attention of many stakeholders, yet cohesive opportunities to deploy in residential sectors, specifically detached single-family dwellings, is scattered. As a result, this study of literature and implementation strategies through simple examples looks to identify several characteristics related to BIPV. Characteristics that were studied in this initial pilot study were design considerations for system selection, applicability to residential construction, and system and material options and enhancements. A case-study home was analyzed demonstrating opportunity for implementation of BIPV on an existing residence. Strategies for maximizing the energy-generating capacity of the system to achieve net-zero energy performance, including all building surfaces and landscaping were also explored. This body of work provides a state-of-the-art review on common materials as well as the more customizable types.

Micro/nano Indentation and Single Grit Diamond Grinding Mechanism on Ultra Pure Fused Silica

The existing research about ductile grinding of fused silica glass was mainly focused on how to carry out ductile regime material removal for generating very ＂smoothed＂ surface and investigate the machining-induced damage in the grinding in order to reduce or eliminate the subsurface damage.The brittle/ductile transition behavior of optical glass materials and the wear of diamond wheel are the most important factors for ductile grinding of optical glass.In this paper,the critical brittle/ductile depth,the influence factors on brittle/ductile transition behavior,the wear of diamond grits in diamond grinding of ultra pure fused silica（UPFS） are investigated by means of micro/nano indentation technique,as well as single grit diamond grinding on an ultra-stiff machine tool,Tetraform ＂C＂.The single grit grinding processes are in-process monitored using acoustic emission（AE） and force dynamometer simultaneously.The wear of diamond grits,morphology and subsurface integrity of the machined groves are examined with atomic force microscope（AFM） and scanning electron microscope（SEM）.The critical brittle/ductile depth of more than 0.5 μm is achieved.When compared to the using roof-like grits,by using pyramidal diamonds leads to higher critical depths of scratch with identical grinding parameters.However,the influence of grit shapes on the critical depth is not significant as supposed.The grinding force increased linearly with depth of cut in the ductile removal regime,but in brittle removal regime,there are large fluctuations instead of forces increase.The SEM photographs of the cross-section profile show that the median cracks dominate the crack patterns beneath the single grooves.Furthermore,The SEM photographs show multi worn patterns of diamond grits,indicating an inhomogeneous wear mechanism of diamond grits in grinding of fused silica with diamond grinding wheels.The proposed research provides the basal technical theory for improving the ultra-precision grinding of UPFS.

Electrically driven single-photon sources

Single-photon sources are building blocks for photonic quantum information processes. Of the many single-photon generation schemes, electrically driven single-photon sources have the advantages of realizing monolithic integration of quantum light sources and detectors without optical filtering, thus greatly simplify the integrated quantum photonic circuits. Here, we review recent advances on electrically driven single-photon sources based on solid-state quantum emitters, such as semiconductor epitaxial quantum dots, colloidal quantum dots, carbon nanotubes, molecules, and defect states in diamond, SiC and layered semiconductors. In particular, the merits and drawbacks of each system are discussed. Finally, the article is concluded by discussing the challenges that remain for electrically driven single-photon sources.

14位Single-slope ADC行为级建模与仿真

单斜率型模/数转换器以其简单的结构、较高的分辨率和易于集成的优势,在红外焦平面读出电路设计中被广泛应用。基于Matlab软件环境下的Simulink工具,建立了一个14位Single-slope ADC的系统模型。其充分讨论Simulink工具下电路各单元模块的具体实现和信号间的时序关系,给出电路的行为级仿真结果,为Single-slope ADC的集成电路设计与实现提供参考。

Integration Possibility of Urban Public Bus System and Cable Car in Maribor

Combination of a bus system and cable car system can reduce the overall congestion of traffic in urban areas, where surrounding hills or mountains hold larger settlements or tourist and recreational infrastructure. With this kind of integration number of individual car trips can be significantly reduced. In this paper, the authors present an analysis of the pilot project implementation, which was held in Maribor. The authors conducted a limited test trial of two means of transportation, combining them into a single operating transport offer for inhabitants and tourists. Combined transport option proved to be a good starting point for reduction of traffic and parking congestion during winter tourist season and beyond. Method used in the research, in order to gain actual potential of integrating two systems and improving public transport offer, was establishment and implementation of the pilot project in Maribor during January 2011. Data was gathered through interviews of two interest groups. The first covered the users who were brought to the foothills of Pohorje＇s ski center by bus. The second covered the cable car users that were traveling to the top of Pohorje. For a limited time period, a trial principle of a single ticket was established, which gave ski-pass holders free bus ride on bus line No. 6. With the aim of reducing CO2, test drives of hybrid bus and compressed natural gas bus were conducted alongside many promotional activities with which users were informed of importance of environmentally friendly mobility options.

Improved single-neuron PID controller for DC motor applied in robot system

An improved single-neuron proportional integral derivative （ PID ） controller and a new method to build the DC motor system were presented in the article. In the simulation, the robot arm is considered as an external load to DC motor. Both the motor module and the load module are crea- ted in Simulink to achieve simulation results closer to real robot system. In this way, it can well veri- fy the performance of the improved single-neuron PID controller, which is a combined controller of normal PID controller and single-neuron PID controller. Besides, an intelligent switcher can help to realize the function of choosing a better control algorithm according to motor＇ s velocity output. Sim- ulated results confirm the rapid and stable response of the improved PID controller. Moreover, the improved single-neuron PID controller has an excellent ability to overcome the load impact and su- press the jamming signals. At last, a GUI interface platform is built to make the controller easier to be applied in other robot systems.

Design and application of single-antenna GPS/accelerometers attitude determination system

In view of the problem that the current single-antenna GPS attitude determination system can only determine the body attitude when the sideslip angle is zero and the multiantenna GPS/SINS integrated navigation system is of large volume, high cost, and complex structure, this approach is presented to determine the attitude based on vector space with single-antenna GPS and accelerometers in the micro inertial measurement unit （MIMU）. It can provide real-time and accurate attitude information. Subsequently, the single-antenna GPS/SINS integrated navigation system is designed based on the combination of position, velocity, and attitude. Finally the semi- physical simulations of single-antenna GPS attitude determination system and single-antenna GPS/SINS integrated navigation system are carried out. The simulation results, based on measured data, show that the single-antenna GPS/SINS system can provide more accurate navigation information compared to the GPS/SINS system, based on the combination of position and velocity. Furthermore, the single-antenna GPS/SINS system is characteristic of lower cost and simpler structure. It provides the basis for the application of a single-antenna GPS/SINS integrated navigation system in a micro aerial vehicle （MAV）.

A Single-Chip UHF RFID Reader Transceiver IC

A single-chip UHF RFID reader transceiver IC has been implemented in 0.18 μm SiGe BiCMOS technology. The chip includes all transceiver blocks as RX/TX RF front-end, RX/TX analog baseband, frequency synthesizer and I2C with fully-compliant China 800/900 MHz RFID draft, ISO/IEC 18000-6C protocol and ETSI 302 208-1 local regulation. The normal mode receiver in the presence of -3 dBm self-jammer achieves -75 dBm 1% PER sensitivity. The linear class-A PA integrated in transmitter has 25 dBm OP1 dB output power for CW. The fully-integrated fractional-N fre-quency synthesizer is designed based on MASH 1-1-1 sigma-delta modulator and 1.8 GHz fundamental frequency LC-VCO for lower in-band and out-of-band phase noise. The measured phase noise is up to -106 dBc/Hz@200 kHz and -131 dBc/Hz@1 MHz offset from center frequency and the integrated RMS jitter from 10 kHz to 10 MHz is less than 1.6 pS. The chip dissipates 330 mA from 3.3 V power supply when transmitting 22.4 dBm CW signal and the PAE of linear PA is up to 26%. The chip die area is 16.8 mm2.

Integrating Color and Spatial Feature for Content-Based Image Retrieval

In this paper, we present a novel and efficient scheme for extracting, indexing and retrieving color images. Our motivation was to reduce the space overhead of partition-based approaches taking advantage of the fact that only a relatively low number of distinct values of a particular visual feature is present in most images. To extract color feature and build indices into our image database we take into consideration factors such as human color perception and perceptual range, and the image is partitioned into a set of regions by using a simple classifying scheme. The compact color feature vector and the spatial color histogram, which are extracted from the seqmented image region, are used for representing the color and spatial information in the image. We have also developed the region-based distance measures to compare the similarity of two images. Extensive tests on a large image collection were conducted to demonstrate the effectiveness of the proposed approach.

Integrative Analysis Confirmed the Association between Osteoprotegerin and Osteoporosis

Objective This study aimed to verify the association between osteoprotegerin gene (OPG) and its variants with osteoporosis (OP) by performing integrative analysis.Methods We used the KGG software to perform gene-based association analysis,which integrated all publicly available single-nucleotide polymorphism (SNP)-based P values and obtained an overall P value for the OPG.The significant SNPs were screened for expression quantitative trait loci (eQTLs).Meta-analysis was used to combine the associations between the variants of OPG and bone mineral density (BMD) reported in the literatures.Then we performed dual-luciferase reporter gene systems for the functional verification of the variants of OPG in vitro.Results In the gene-based association analysis,the over all P value of OPG was 6.24×10^-13 for BMD at femoral neck (FN) and 7.37×10^-17 for BMD at lumbar spine (LS),indicating the importance of OPG for OP.The publicly available eQTL database identified 5 eQTLs which exert cis-regulation effects on OPG at FN and LS.Literature searching found that rs2073617 (known as T950C) was the hot spot SNP.There were 13 relevant studies on rs2073617 besides the GEFOS-2 study identified from the PubMed.Significant differences among TT,TC and CC genotypes at FN (P= 0.047) and LS (P= 0.025) were shown by meta-analysis,demonstrating the associations between T950C polymorphism and BMD.Luciferase gene expression was significantly higher at the presence of allele C than allele T in the 293T cells (t=-9.47,P<0.01).Conclusion The integrative analysis further confirmed the importance of OPG in OP and the correlation of T950C polymorphism with BMD of OP.The strategy can be used as a reference for functional interpretation of other disease-related genes.

Integrated Control System of Vehicle ABS/ASR/ACC Based onMC9S12DP256

The integrated control system of vehicle ABS/ASR/ACC has been developed using the MC9S12DP256 single chip, which is the new Motorola 16-bit product in HSC12 family. The system including the main control module, the data collection module and the drive and fault diagnosis module is demonstrated and its data collection function is presented in detail. The system designed by the modularization can supervise the data, drive the valves and pump. The program can be debugged on line, which is steady and reliable validated by the large numbers of vehicle road tests.