Objective To assess the effect of benzene on sperm DNA damage ;Methods Twenty-seven benzene-exposed workers were selected as exposed group and 35 normal sperm donors as control group. Air concentration of benzene seri...Objective To assess the effect of benzene on sperm DNA damage ;Methods Twenty-seven benzene-exposed workers were selected as exposed group and 35 normal sperm donors as control group. Air concentration of benzene series in workshop was determined by gas chromatography. As an internal exposure dose of benzene, the concentration of trans, trans-muconic acid （ttMA） was determined by high performance liquid chromatography. DNA was detected by modified single cell gel electrophoresis （SCGE）. Results The air concentrations of benzene, toluene and xylene at the workplace were 86.49±2.83 mg/m^3, 97.20±3.52 mg/m^3 and 97.45± 2.10 mg/m^3, respectively. Urinary ttMA in exposed group （1.040 ± 0.617 mg/L） was significantly higher than that of control group （0.819 ± 0.157 mg/L）. The percentage of head DNA, determined by modified SCGE method, significantly decreased in the exposed group （n=13, 70.18% ± 7.36%） compared with the control （n=16, 90.62% ± 2.94%）（P〈0.001）. Conclusion The modified SCGE method can be used to investigate the damage of sperm DNA. As genotoxin and reprotoxins, benzene had direct effect on the germ cells during the spermatogenesiss.展开更多
Objective:To detect the integrity of sperm DNA in the catfish Pangasianodon hypophthalmus cryopreserved with Hanks balanced salt solution,5%-30%dimethyl acetamide(DMA)using the single-cell gel electrophoresis in order...Objective:To detect the integrity of sperm DNA in the catfish Pangasianodon hypophthalmus cryopreserved with Hanks balanced salt solution,5%-30%dimethyl acetamide(DMA)using the single-cell gel electrophoresis in order to test how sperm cryopreservation affected nuclear DNA stability.Methods:Electrophoresis was conducted for 60 min at 130 mA and 15 V.The comet images were analyzed using software CometScore 1.5,and parameters such as comet length,tail length and percentage DNA in the tail were obtained.Then the comet rate and damage coefficient were calculated.Results:There were no significant differences in motility,comet rate and damage coefficient between fresh sperm and cryopreserved sperm stored in 5%,10%,15%and 20%DMA,while the sperm cryopreserved with 25%and 30%DMA had lower motility,higher comet length and damage coefficients than those of fresh sperm.Conclusions:There was positive correlation between comet rate of cryopreserved sperm and the concentration of DMA.The toxicity of cryoprotectant is the main factor for DNA damage in cryopreserved sperm.展开更多
文摘Objective To assess the effect of benzene on sperm DNA damage ;Methods Twenty-seven benzene-exposed workers were selected as exposed group and 35 normal sperm donors as control group. Air concentration of benzene series in workshop was determined by gas chromatography. As an internal exposure dose of benzene, the concentration of trans, trans-muconic acid （ttMA） was determined by high performance liquid chromatography. DNA was detected by modified single cell gel electrophoresis （SCGE）. Results The air concentrations of benzene, toluene and xylene at the workplace were 86.49±2.83 mg/m^3, 97.20±3.52 mg/m^3 and 97.45± 2.10 mg/m^3, respectively. Urinary ttMA in exposed group （1.040 ± 0.617 mg/L） was significantly higher than that of control group （0.819 ± 0.157 mg/L）. The percentage of head DNA, determined by modified SCGE method, significantly decreased in the exposed group （n=13, 70.18% ± 7.36%） compared with the control （n=16, 90.62% ± 2.94%）（P〈0.001）. Conclusion The modified SCGE method can be used to investigate the damage of sperm DNA. As genotoxin and reprotoxins, benzene had direct effect on the germ cells during the spermatogenesiss.
基金Supported by UGC-SAP-DSA(Grant No.SR/SO/As-03/2003).
文摘Objective:To detect the integrity of sperm DNA in the catfish Pangasianodon hypophthalmus cryopreserved with Hanks balanced salt solution,5%-30%dimethyl acetamide(DMA)using the single-cell gel electrophoresis in order to test how sperm cryopreservation affected nuclear DNA stability.Methods:Electrophoresis was conducted for 60 min at 130 mA and 15 V.The comet images were analyzed using software CometScore 1.5,and parameters such as comet length,tail length and percentage DNA in the tail were obtained.Then the comet rate and damage coefficient were calculated.Results:There were no significant differences in motility,comet rate and damage coefficient between fresh sperm and cryopreserved sperm stored in 5%,10%,15%and 20%DMA,while the sperm cryopreserved with 25%and 30%DMA had lower motility,higher comet length and damage coefficients than those of fresh sperm.Conclusions:There was positive correlation between comet rate of cryopreserved sperm and the concentration of DMA.The toxicity of cryoprotectant is the main factor for DNA damage in cryopreserved sperm.
