In recent decades,single-cell(SC)technologies and applications have been a very hot topics in the field of biology and medicine.In fact,early studies involving a single cell were on the malignant process of acute nonl...In recent decades,single-cell(SC)technologies and applications have been a very hot topics in the field of biology and medicine.In fact,early studies involving a single cell were on the malignant process of acute nonlymphocytic leukemia and inherited or sporadic genetic disease in the 1980s.And since the RhD gene in a single cell from maternal plasma was detected by fluorescence-based polymerase chain reaction(PCR)in the 1990s,“SC biopsies”[1]were widely used for early diagnosis in clinical practice in the 2000s.Previous studies showed that SC RNA sequencing(scRNA-seq)was used to evaluate patterns of allelic gene expression at the allele-specific mRNA level,and SC genome analysis can be used for genetic diagnosis.展开更多
文摘In recent decades,single-cell(SC)technologies and applications have been a very hot topics in the field of biology and medicine.In fact,early studies involving a single cell were on the malignant process of acute nonlymphocytic leukemia and inherited or sporadic genetic disease in the 1980s.And since the RhD gene in a single cell from maternal plasma was detected by fluorescence-based polymerase chain reaction(PCR)in the 1990s,“SC biopsies”[1]were widely used for early diagnosis in clinical practice in the 2000s.Previous studies showed that SC RNA sequencing(scRNA-seq)was used to evaluate patterns of allelic gene expression at the allele-specific mRNA level,and SC genome analysis can be used for genetic diagnosis.
