Physical biology is an interdisciplinary field that bridges biology with physical sciences and engineering.Singlemolecule physical biology focuses on dynamics of individual biomolecules and complexes,aiming to answeri...Physical biology is an interdisciplinary field that bridges biology with physical sciences and engineering.Singlemolecule physical biology focuses on dynamics of individual biomolecules and complexes,aiming to answering basic questions about their functions and mechanisms.It takes advantages of physical methodologies to gain quantitative understanding of biological processes,often engaging precise physical measurements of reconstructed objects to avoid interference from unnecessary complications.In this review,we(i)briefly introduce concepts of single-molecule physical biology,(ii)describe extensively used single-molecule methodologies that have been developed to address key questions in two important objects of single-molecule physical biology,namely,nucleic acid-interacting proteins and membraneinteracting proteins,and(iii)show by a few successful examples how one may use single-molecule methods to deepen our understanding of protein machines.展开更多
In relation to human issues,the relationship between biology and culture has long been an important part of general social science research,cultural studies in particular.In which Chris Barker’s theory of biology and...In relation to human issues,the relationship between biology and culture has long been an important part of general social science research,cultural studies in particular.In which Chris Barker’s theory of biology and culture in relation to related theories can be considered as one of the noteworthy remarks about the method and methodology to pass on the actual field Nam Huynh Dao school,the article focuses on clarifying the basic content of the problem with orientation to contribute to research and development of physical culture in Vietnam at present.This article was selected to read and print in the conference proceedings of scientific conference entitled“Theory of Western Culture and Applied to Vietnamese Culture Research”organized by the University of Social Sciences and Humanities,Vietnam National University-HCM City(USSH,VNU-HCM,Vietnam)(29 November 2018).展开更多
Processes for decoding the genetic information in cells, including transcription, replication, recombi- nation and repair, involve the deformation of DNA from its equilibrium structures such as bending, stretching, tw...Processes for decoding the genetic information in cells, including transcription, replication, recombi- nation and repair, involve the deformation of DNA from its equilibrium structures such as bending, stretching, twisting, and unzipping of the double helix. Single-molecule manipulation techniques have made it possible to control DNA conformation and simultaneously detect the induced changes, revealing a rich variety of mechanically-induced conformational changes and thermodynamic states. These single-molecule techniques helped us to reveal the physics of DNA and the processes involved in the passing on of the genetic code.展开更多
基金supported by the National Key Research and Development Program of China(Grant No.2019YFA0709304)the National Natural Science Foundation of China(Grant Nos.12090051 and 12022409)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB37000000)the Youth Innovation Promotion Association of CAS(Grant Nos.2021009 and Y2021003)
文摘Physical biology is an interdisciplinary field that bridges biology with physical sciences and engineering.Singlemolecule physical biology focuses on dynamics of individual biomolecules and complexes,aiming to answering basic questions about their functions and mechanisms.It takes advantages of physical methodologies to gain quantitative understanding of biological processes,often engaging precise physical measurements of reconstructed objects to avoid interference from unnecessary complications.In this review,we(i)briefly introduce concepts of single-molecule physical biology,(ii)describe extensively used single-molecule methodologies that have been developed to address key questions in two important objects of single-molecule physical biology,namely,nucleic acid-interacting proteins and membraneinteracting proteins,and(iii)show by a few successful examples how one may use single-molecule methods to deepen our understanding of protein machines.
文摘In relation to human issues,the relationship between biology and culture has long been an important part of general social science research,cultural studies in particular.In which Chris Barker’s theory of biology and culture in relation to related theories can be considered as one of the noteworthy remarks about the method and methodology to pass on the actual field Nam Huynh Dao school,the article focuses on clarifying the basic content of the problem with orientation to contribute to research and development of physical culture in Vietnam at present.This article was selected to read and print in the conference proceedings of scientific conference entitled“Theory of Western Culture and Applied to Vietnamese Culture Research”organized by the University of Social Sciences and Humanities,Vietnam National University-HCM City(USSH,VNU-HCM,Vietnam)(29 November 2018).
文摘Processes for decoding the genetic information in cells, including transcription, replication, recombi- nation and repair, involve the deformation of DNA from its equilibrium structures such as bending, stretching, twisting, and unzipping of the double helix. Single-molecule manipulation techniques have made it possible to control DNA conformation and simultaneously detect the induced changes, revealing a rich variety of mechanically-induced conformational changes and thermodynamic states. These single-molecule techniques helped us to reveal the physics of DNA and the processes involved in the passing on of the genetic code.
