Status of research on non-conventional technology assisted single-point diamond turning

With the increasing use of difficult-to-machine materials in aerospace applications,machining requirements are becoming ever more rigorous.However,traditional single-point diamond turning(SPDT)can cause surface damage and tool wear.Thus,it is difficult for SPDT to meet the processing requirements,and it has significant limitations.Research indicates that supplementing SPDT with unconventional techniques can,importantly,solve problems due to the high cutting forces and poor surface quality for difficult-to-machine materials.This paper first introduces SPDT and reviews research into unconventional techniques for use with SPDT.The machining mechanism is discussed,and the main advantages and disadvantages of various methods are investigated.Second,hybrid SPDT is briefly described,which encompasses ultrasonic-vibration magnetic-field SPDT,ultrasonic-vibration laser SPDT,and ultrasonic-vibration cold-plasma SPDT.Compared with the traditional SPDT method,hybrid SPDT produces a better optical surface quality.The current status of research into unconventional techniques to supplement SPDT is then summarized.Finally,future development trends and the application prospects of unconventional assisted SPDT are discussed.

Analysis for the Deployment of Single-Point Mooring Buoy System Based on Multi-Body Dynamics Method

Deployment of buoy systems is one of the most important procedures for the operation of buoy system. In the present study, a single-point mooring buoy system which contains surface buoy, cable segments with components, anchor and so on is modeled by applying multi-body dynamics method. The motion equations are developed in discrete node description and fully Cartesian coordinates. Then numerical method is used to solve the ordinary differential equations and dynamics simulations are achieved while anchor is casting from board. The trajectories and velocities of different nodes without current and with current in buoy system are obtained. The transient tension force of each part of the cable is analyzed in the process of deployment. Numerical results indicate that the transient payload increases to a peak value when the anchor is touching the seabed and the maximum tension force will vary with different floating configuration. This work is helpful for design and deployment planning of buoy system.

The Structure of Background-error Covariance in a Four-dimensional Variational Data Assimilation System:Single-point Experiment

A four dimensional variational data assimilation （4DVar） based on a dimension-reduced projection （DRP-4DVar） has been developed as a hybrid of the 4DVar and Ensemble Kalman filter （EnKF） concepts. Its good flow-dependent features are demonstrated in single-point experiments through comparisons with adjointbased 4DVar and three-dimensional variational data （3DVar） assimilations using the fifth-generation Pennsylvania State University-National Center for Atmospheric Research Mesoscale Model （MM5）. The results reveal that DRP-4DVar can reasonably generate a background error covariance matrix （simply B-matrix） during the assimilation window from an initial estimation using a number of initial condition dependent historical forecast samples. In contrast, flow-dependence in the B-matrix of MM5 4DVar is barely detectable. It is argued that use of diagonal estimation in the B-matrix of the MM5 4DVar method at the initial time leads to this failure. The experiments also show that the increments produced by DRP-4DVar are anisotropic and no longer symmetric with respect to observation location due to the effects of the weather trends captured in its B-matrix. This differs from the MM5 3DVar which does not consider the influence of heterogeneous forcing on the correlation structure of the B-matrix, a condition that is realistic for many situations. Thus, the MM5 3DVar assimilation could only present an isotropic and homogeneous structure in its increments.

Numerical Simulation of Azimuthal Uniformity of Injection Currents in Single-Point-Feed Induction Voltage Adders

In order to investigate the injection current uniformity around the induction cell bores, two fully electromagnetic （EM） models are respectively established for a single-stage induction cell and an induction voltage adder （IVA） with three cells stacked in series, without considering electron emission. By means of these two models, some factors affecting the injection current uni- formity are simulated and analyzed, such as the impedances of adders and loads, cell locations, and feed timing of parallel driving pulses. Simulation results indicate that higher impedances of adder and loads are slightly beneficial to improve injection current uniformity. As the impedances of adder and loads increase from 5 Ω to 30Ω, the asymmetric coefficient of feed currents decreases from 10.3% to 6.6%. The current non-uniformity within the first cell is a little worse than that in other downstream cells. Simulation results also show that the feed timing would greatly affect current waveforms, and consequently cause some distortion in pulse fronts of cell output voltages. For a given driving pulse with duration time of 70-80 ns, the feed timing with a time deviation of less than 20 ns is acceptable for the three-cell IVAs, just causing the rise time of output voltages to increase about 5 ns at most and making the peak voltage decrease by 3.5%.

A Guide to Cheek Augmentation: Single-Point Deep Injection of Hyaluronic Acid Filler at Midface in Close Proximity to Medial Suborbicularis Oculi Fat (SOOF) Area

Loss of volume in midface can result in an aged, wasted appearance. Osseous and fat atrophy with aging may further contribute to the loss of soft tissue support and midface ptosis. In the aging of periorbital area and midface, fat atrophy occurs mostly in the suborbicularis oculi fat (SOOF) area. The authors proposed that injection of hyaluronic acid (HA) filler to support the SOOF area could counteract the aging sign due to fat atrophy, restore volume loss and achieve a more youthful appearance. The authors described the treatment of 10 female patients who received CHAP®-particle hyaluronic acid (CHAP®-HA) injections for cheek augmentation, using single-point deep injection technique at midface in close proximity to SOOF area. Such approach provides satisfactory cheek augmentation results without significant complications. The authors discussed a rationale for their choice of dermal filler and provided an injection technique for restoring volume in the midface region with CHAP®-HA. Such technique is relatively quick to perform, have little down time, and result in a high rate of patient satisfaction.

A Simulated Investigation of Ductile Response of GaAs in Single-Point Diamond Turning and Experimental Validation

In this paper,molecular dynamic(MD)simulation was adopted to study the ductile response of single-crystal GaAs during single-point diamond turning(SPDT).The variations of cutting temperature,coordination number,and cutting forces were revealed through MD simulations.SPDT experiment was also carried out to qualitatively validate MD simulation model from the aspects of normal cutting force.The simulation results show that the fundamental reason for ductile response of GaAs during SPDT is phase transition from a perfect zinc blende structure(GaAs-I)to a rock-salt structure(GaAs-II)under high pressure.Finally,a strong anisotropic machinability of GaAs was also found through MD simulations.

Profile Compensation for Single-Point Diamond Turning of Microlens Array

To improve the efficiency and consistency of machined microlens array using single-point diamond turning technology,a theoretical model of surface form error is proposed in this paper.Then,a compensation method for this model is studied.In the proposed tool equivalent tilt angle model,the microlens array is regarded as a freeform surface.The corresponding curvature radius of the surface at each cutting point along the cutting direction is calculated by establishing a slow slide servo cutting model.In the spatial form error model,the assumption is that surface form error has a linear relationship with z-axis maximum speed vz.An empirical linear equation is obtained and verified,with a maximum deviation of 0.4μm.Then,after machining,the surface form error is measured and processed using on-machine measurement.The theoretical and measured surface form errors are consistent.The surface form error is compensated in the machining program.The peak-to-valley value is reduced from 5.4 to 0.6μm after compensation.Findings show that the single-point diamond turning and compensation method for the microlens array presented in this paper can predict the surface form error and significantly improve machining accuracy and consistency.

Analysis and Prediction of Effect of Turning Marks Diffraction on Image Quality of Optical System

Single-point diamond turning (SPDT) is widely used in the machining of infrared materials and metal-based mirrors. Diamond tips can scratch material, replicate the shape of the tip, and create annular turning marks on optical surfaces, which can have unpredictable adverse effects on imaging. In order to predict the effect of turning marks diffraction on the degradation of imaging quality, a model of the influence of SPDT processing parameters on the reduction of system imaging MTF under the influence of ideal grating turning marks diffraction was established. The results show that the depth of the turning mark will lead to the decline of MTF, especially the low frequency information. Finally, a method is proposed to reduce the effect of turning marks diffraction through changing the processing parameters. .

The group search-based parallel algorithm for the serial Monte Carlo inversion method

With the development of parallel computing technology,non-linear inversion calculation efficiency has been improving.However,for single-point search-based non-linear inversion methods,the implementation of parallel algorithms is a difficult issue.We introduce the idea of group search to the single-point search-based non-linear inversion algorithm, taking the quantum Monte Carlo method as an example for two-dimensional seismic wave velocity inversion and practical impedance inversion and test the calculation efficiency of using different node numbers.The results show the parallel algorithm in theoretical and practical data inversion is feasible and effective.The parallel algorithm has good versatility. The algorithm efficiency increases with increasing node numbers but the algorithm efficiency rate of increase gradually decreases as the node numbers increase.

Structural analysis of tumor-relatedsingle amino acid mutations in human MxA protein

Background:Human myxovirus resistant protein A(MxA),encoded by the myxovirus resistance 1(Mx1) gene,is an interferon(IFN)-triggered dynamin-like multi-domain GTPase involved in innate immune responses against viral infections.Recent studies suggest that MxA is associated with several human cancers and may be a tumor suppressor and a promising biomarker for IFN therapy.Mxl gene mutations in the coding region for MxA have been discovered in many types of cancer,suggesting potential biological associations between mutations in MxA protein and corresponding cancers.In this study,we performed a systematic analysis based on the crystal structures of MxA and elucidated how these mutations specifically affect the structure and therefore the function of MxA protein.Methods:Cancer-associated Mxl mutations were collected and screened from the COSMIC database.Twenty-two unique mutations that cause single amino acid alterations in the MxA protein were chosen for the analysis.Amino acid sequence alignment was performed using Clustal W to check the conservation level of mutation sites in Mx proteins and dynamins.Structural analysis of the mutants was carried out with Coot.Structural models of selected mutants were generated by the SWISS-MODEL server for comparison with the corresponding non-mutated structures.All structural figures were generated using PyMOL.Results:We analyzed the conservation level of the single-point mutation sites and mapped them on different domains of MxA.Through individual structural analysis,we found that some mutations severely affect the stability and function of MxA either by disrupting the intraVinter-molecular interactions supported by the original residues or by incurring unfavorable configuration alterations,whereas other mutations lead to gentle or no interference to the protein stability and function because of positions or polarity features.The potential clinical value of the mutations that lead to drastic influence on MxA protein is also assessed.Conclusions:Among all of the reported tumor-associated single-point mutations,seven of them notably affect the structure and function of MxA and therefore deserve more attention with respect to potential clinical applications.Our research provides an example for systematic analysis and consequence evaluation of single-point mutations on a given cancer-related protein.

Enhancement of surface wettability via micro-and nanostructures by single point diamond turning

Studies on surface wettability have received tremendous interest due to their potential applications in research and industrial processes. One of the strategies to tune surface wettability is modifying surface topography at micro-and nanoscales. In this research, periodic micro-and nanostructures were patterned on several polymer surfaces by ultra-precision single point diamond turning to investigate the relationships between surface topographies at the micro-and nanoscales and their surface wettability. This research revealed that single-point diamond turning could be used to enhance the wettability of a variety of polymers, including polyvinyl chloride(PVC), polyethylene 1000(PE1000), polypropylene copolymer(PP) and polytetrafluoroethylene(PFTE), which cannot be processed by conventional semiconductor-based manufacturing processes. Materials exhibiting common wettability properties(θ≈ 90°) changed to exhibit "superhydrophobic" behavior(θ > 150°). Compared with the size of the structures, the aspect ratio of the void space between micro-and nanostructures has a strong impact on surface wettability.

New real-time-PCR method to identify single point mutations in hepatitis C virus

AIM To develop a fast, low-cost diagnostic strategy to identify single point mutations in highly variable genomes such as hepatitis C virus(HCV).METHODS In patients with HCV infection, resistance-associated amino acid substitutions within the viral quasispecies prior to therapy can confer decreased susceptibility to direct-acting antiviral agents and lead to treatment failure and virological relapse. One such naturally occurring mutation is the Q80 K substitution in the HCV-NS3 protease gene, which confers resistance to PI inhibitors, particularly simeprevir. Low-cost, highly sensitive techniques enabling routine detection of these single point mutations would be useful to identify patients at a risk of treatment failure. Light Cycler methods, based on real-time PCR with sequencespecific probe hybridization, have been implemented in most diagnostic laboratories. However, this technique cannot identify single point mutations in highly variable genetic environments, such as the HCV genome. To circumvent this problem, we developed a new method to homogenize all nucleotides present in a region except the point mutation of interest. RESULTS Using nucleotide-specific probes Q, K, and R substitutions at position 80 were clearly identified at a sensitivity of 10%(mutations present at a frequency of at least 10% were detected). The technique was successfully applied to identify the Q80 K substitution in 240 HCV G1 serum samples, with performance comparable to that of direct Sanger sequencing, the current standard procedure for this purpose. The new method was then validated in a Catalonian population of 202 HCV G1-infected individuals. Q80 K was detected in 14.6% of G1 a patients and 0% of G1 b in our setting. CONCLUSION A fast, low-cost diagnostic strategy based on real-time PCR and fluorescence resonance energy transfer probe melting curve analysis has been successfully developed to identify single point mutations in highly variable genomes such as hepatitis C virus. This technique can be adapted to detect any single point mutation in highly variable genomes.

Analysis on the Seismic Response of Soil Slopes Based on the Multi-point Input Method

In general, the seismic response analysis in earthquake engineering assumes that the vibration parameters of the target and the contact surface of the external media are identical,i. e., single point input. However, earthquake energy has an attenuation phenomenon in wave propagation,so a wide range of soil slopes and the external medium contact surface of different input points on motion are not identical. If we consider single point input only, it may not correspond with reality, so it is necessary to carry out research on multi-point input methods. Based on the 2-D slope model,single-point input and multi-point input are performed respectively to analyze and compare their similarities and differences in the perspectives of the characteristics of seismic response of soil layer and plastic zone distribution to provide a reference for the seismic design of slopes. The results show that in the perspective of soil seismic response analysis,the peak acceleration output and peak velocity output under multi-point input are greater than the peak values under single point input at the same monitoring point,the peak appearing time is also earlier than that of the single point input; in terms of the plastic zone distribution,the multi-point effect is manifested as the presence of more obvious tensile shear failures; in the perspective of safety coefficient,the safety coefficient under each multi-point input is smaller than that of single point input,a difference of about 7 % or so. In summary,multi-point input is more reasonable and practical than single point input,so multi-point input should be considered in seismic design.

A Fast Tool Servo System for Fabrication of Micro-structured Surfaces on A Diamond Turning Machine

A fast tool servo (FTS) system is developed for the fabrication of non-rotationally symmetric micro-structured surfaces using single-point diamond turning machines.The constructed FTS employs a piezoelectric tube actuator (PZT) to actuate the diamond tool and a capacitive probe as the feedback sensor.To compensate the inherent nonlinear hysteresis behavior of the piezoelectric actuator,Proportional Integral (PI) feedback control is implemented.Besides,a feed-forward control based on a simple feed-forward predictor has been added to achieve better tracking performance.Experimental results indicate that error motions in the performance of the system caused by hysteresis can be reduced greatly and the micro-structured surface is successfully fabricated by implementing the FTS.

Zeroth-Order Methods for Online Distributed Optimization with Strongly Pseudoconvex Cost Functions

This paper studies an online distributed optimization problem over multi-agent systems.In this problem,the goal of agents is to cooperatively minimize the sum of locally dynamic cost functions.Different from most existing works on distributed optimization,here we consider the case where the cost function is strongly pseudoconvex and real gradients of objective functions are not available.To handle this problem,an online zeroth-order stochastic optimization algorithm involving the single-point gradient estimator is proposed.Under the algorithm,each agent only has access to the information associated with its own cost function and the estimate of the gradient,and exchange local state information with its immediate neighbors via a time-varying digraph.The performance of the algorithm is measured by the expectation of dynamic regret.Under mild assumptions on graphs,we prove that if the cumulative deviation of minimizer sequence grows within a certain rate,then the expectation of dynamic regret grows sublinearly.Finally,a simulation example is given to illustrate the validity of our results.

Sub-nanometer finishing of polycrystalline tin by inductively coupled plasma-assisted cutting

Polycrystalline tin is an ideal excitation material for extreme ultraviolet light sources.However,the existence of grain boundary(GB)limits the surface roughness of polycrystalline tin after single-point diamond turning(SPDT).In this work,a novel method termed inductively coupled plasma(ICP)-assisted cutting was developed for the sub-nanometer finishing of polycrystalline tin.The relationship between ICP power,processing time,and modification depth was established by thermodynamic simulation,and the fitted heat transfer coefficient of polycrystalline tin was 540 W/(m2·K).The effects of large-thermal-gradient ICP treatment on the microstructure of polycrystalline tin were studied.After 0.9 kW ICP processing for 3.0 s,corresponding to the temperature gradient of 0.30 K/μm,the grain size of polycrystalline tin was expanded from a size of approximately 20-80μm to a millimeter scale.The Taguchi method was used to investigate the effects of rotational speed,depth of cut,and feed rate on SPDT.Experiments conducted based on the ICP system indicated that the plasma-assisted cutting method promoted the reduction of the influence of GB steps on the finishing of polycrystalline tin,thereby achieving a surface finish from 8.53 to 0.80 nm in Sa.The results of residual stress release demonstrated that the residual stress of plasma-assisted turning processing after 504 h stress release was 10.7 MPa,while that of the turning process without the ICP treatment was 41.6 MPa.

Recent advancements in optical microstructure fabrication through glass molding process

Optical microstructures are increasingly applied in several fields, such as optical systems, precision measurement, and microfluid chips. Microstructures include microgrooves, microprisms, and microlenses. This paper presents an overview of optical microstructure fabrication through glass molding and highlights the applications of optical microstructures in mold fabrication and glass molding. The glass-mold interface friction and adhesion are also discussed. Moreover, the latest advance- ments in glass molding technologies are detailed, including new mold materials and their fabrication methods, viscoelastic constitutive modeling of glass, and micro- structure molding process, as well as ultrasonic vibration- assisted molding technology.

An examination of the OMIM database for associating mutation to a consensus reference sequence

Gene mutation(e.g.substitution,insertion and deletion)and related phenotype information are important bio-medical knowledge.Many biomedical databases(e.g.OMIM)incorporate such data.However,few studies have examined the quality of this data.In the current study,we examined the quality of protein single-point mutations in the OMIM and identified whether the cor-responding reference sequences align with the muta-tion positions.Our results show that close to 20%of mutation data cannot be mapped to a single reference sequence.The failed mappings are caused by position conflict,site shifting(peptide,N-terminal methionine)and other types of data error.We propose a preliminary model to resolve such inconsistency in the OMIM database.

Modified catalytic performance of Lactobacillus fermentum l-lactate dehydrogenase by rational design

l-Lactate dehydrogenases can reduce alpha-keto carboxylic acids asymmetrically and generally have a broad substrate spectrum.l-Lactate dehydrogenase gene(LF-l-LDH0845)with reducing activity towards 3,4-dihydroxyphenylpyruvate and phenylpyruvate was obtained from Lactobacillus fermentum JN248.To change the substrate specificity of LDH0845 and improve its catalytic activity towards large substrates,site-directed mutation of Tyr221 was performed by analyzing the amino acids in the active center.Kinetic parameters show that the kcat values of Y221F mutant on 3,4-dihydroxyphenylpyruvate,4-methyl-2-oxopentanoate,and glyoxylate are 1.21 s^(−1),1.35 s^(−1),and 0.72 s^(−1),respectively,which are 420%,150%and 130%of the wild-type LDH0845.This study shows that the mutations of Y221 can significantly change the substrate specificity of LDH0845,making it become a potential tool enzyme for the reduction of alpha-keto carboxylic acids with large functional groups.

Coverage analysis of cache-enabled small cell networks with stochastic geometry methods

Caching popular content in the storage of small cells is deemed as an efficient way to decrease latency, offload backhaul and satisfy user＇s demands. In order to investigate the performance of cache-enabled small cell networks, coverage probability is studied in both single-point transmission and cooperative multipoint（Co MP） transmission scenarios. Meanwhile, the caching distribution modeled as Zipf and uniform distribution are both considered. Assuming that small base stations（SBSs） are distributed as a homogeneous Poisson point process（HPPP）, the closed-form expressions of coverage probability are derived in different transmission cases. Simulation results show that Co MP transmission achieves a higher coverage probability than that of single-point transmission. Furthermore, Zipf distribution-based caching is more preferable than uniform distribution-based caching in terms of coverage probability.