XN901 is a K-type three-line hybrid wheat with a high yield potential, and its leaves and stem remaining green during grain maturation, suggesting much assimilate stay in leaves and stem. The grain water content, grai...XN901 is a K-type three-line hybrid wheat with a high yield potential, and its leaves and stem remaining green during grain maturation, suggesting much assimilate stay in leaves and stem. The grain water content, grain volume, carbohydrate content, and enzyme activity of sucose metabolism in the grain, as well as source-sink relationship were studied in order to investigate the physiological reason of the assimilate remaining in leaves and stem at the late stage. The results showed that the hybrid grains had more water and soluble sugar, higher activities of acid invertase and sucrose synthase at the early stage that led to a faster expansion growth, greater grain volume and faster starch synthesis at the early to mid stage of grain development. Also it had a longer period for actively filling. As a result, the grain weight and yield of the hybrid were increased by 14 and 15% respectively compared to that of Shaan 229. Additionally, the biomass of XN901 was 41.7% more than that of control, but its harvest index was 9% lower than Shaan 229. However, its lower activity of sucrose synthase indicated a lower sink activity at the late stage, resulting in a slow rate of filling and starch synthesis. Also, the hybrid wheat XN901 had a large source-sink ratio. It is the main reason for much assimilate remaining in the straw at the late stage and lower harvest index. Strengthening the sink activity and raising the harvest index should be the key means of improving the yield of hybrid wheat.展开更多
Poor filling and low weight of inferior kernels limit the further improvement of wheat yield. Two cultivars, Shuangda 1 and Xinong 538, with different grain weights, were selected to investigate the physiological chan...Poor filling and low weight of inferior kernels limit the further improvement of wheat yield. Two cultivars, Shuangda 1 and Xinong 538, with different grain weights, were selected to investigate the physiological changes of inferior kernels by removal of superior kernels(RS) at the flowering stage. i TRAQ combined with physiological indexes was used to identify factors limiting the filling of inferior kernels.Removal of superior kernels significantly increased the mean filling rate of inferior kernels and thus increased their weight. A set of 6012 proteins in inferior wheat kernels were differentially expressed between the RS and control. These differentially expressed proteins were involved mainly in carbon metabolism and energy metabolism. The main reason for the promoting effect of RS on the filling of inferior kernels may be that RS downregulated proteins involved in glycolysis and pyruvate metabolism while upregulating proteins involved in carbon fixation and photosynthesis. Consequently, RS greatly increased the ATP content in inferior kernels, supplying energy for them to absorb photosynthetic assimilates. Removal of superior kernels increased the activities of sucrose synthase, soluble starch synthase,adenosine diphosphate glucose pyrophosphorylase, and starch branching enzyme in inferior kernels and promoted starch accumulation in them. Thus, RS promoted the filling of inferior kernels and increased their weight. The promoting effect of RS on starch synthesis in inferior kernels was associated with their endogenous IAA and ABA levels.展开更多
Auxin(indole-3-acetic acid, IAA) has a considerable impact on the regulation of plant carbohydrate levels and growth, but the mechanism by which it regulates sugar levels in plants has received little attention. In th...Auxin(indole-3-acetic acid, IAA) has a considerable impact on the regulation of plant carbohydrate levels and growth, but the mechanism by which it regulates sugar levels in plants has received little attention. In this study, we found that exogenous IAA altered fructose(Fru), glucose(Glc), and sucrose(Suc) concentrations in shoot tips mainly by regulating MdSUSY1, MdFRK2, MdHxK1 and MdSDH2 transcript levels. Additionally, we used 5-year-old ’Royal Gala’ apple trees to further verify that these genes play primary roles in regulating sink strength. The results showed that MdSUSY1, MdFRK2, MdHxK1/3 and MdSDH2 might be major contributors to sink strength regulation. Taken together, these results provide new insight into the regulation of the carbohydrate metabolism mechanism, which will be helpful for regulating sink strength and yield.展开更多
青藏高原是中国陆地生态系统的一个重要的碳汇功能区,但其碳汇强度及潜力的估算存在很大的差异,不利于区域碳中和目标的实现。基于涡度相关技术观测的原生高寒草地生态系统CO_(2)通量的40个站点年数据,结合2000年~2018年的年均气温、年...青藏高原是中国陆地生态系统的一个重要的碳汇功能区,但其碳汇强度及潜力的估算存在很大的差异,不利于区域碳中和目标的实现。基于涡度相关技术观测的原生高寒草地生态系统CO_(2)通量的40个站点年数据,结合2000年~2018年的年均气温、年均降水和年最大归一化植被指数(NDVIm)等因子,构建增强回归树模型以研究青海省陆地生态系统碳汇潜力及空间特征。结果表明增强回归树模型能够较好地模拟原生高寒草地碳汇强度的时空变异(R2=0.61),碳汇强度的观测值与模拟值的均方根误差和平均绝对误差分别为33.78 g C/m^(2)和26.63 g C/m^(2)。年均气温和NDVIm是高寒草地碳汇强度时空变异的主要影响因子,二者的相对贡献分别为48.6%和39.0%。青海省陆地生态系统每年的碳汇潜力平均为44.82±22.57 g C/m^(2)(平均值±标准差),高值区集中在海北州的中部及黄南州、果洛州和玉树州的东南部,低值区分布在海西州、海南州和海东市。青海省陆地生态系统每年的碳汇潜力总和为16.60 Mt C,其中高寒草甸和高寒草原分别为11.48 Mt C和3.13 Mt C,是青海省碳汇功能维持和提升的重点保育对象。研究结果可为青海省陆地生态系统的功能评估及率先实现碳中和目标提供数据支撑。展开更多
The design of an effective and robust data gathering algorithm is crucial to the overall performance of wireless sensor networks (WSN). However, using traditional routing algorithms for data gathering is energy-ineffi...The design of an effective and robust data gathering algorithm is crucial to the overall performance of wireless sensor networks (WSN). However, using traditional routing algorithms for data gathering is energy-inefficient for sensor nodes with limited power resources and multi-hop communication protocols. Data gathering with mobile sinks provided an effective solution to this problem. The major drawback of this approach is the time and path constraints of the mobile sink, which limit the mobile sink to collect data from all sensor nodes and, then, data routing is still required for these unreachable parts by the mobile sink. This paper presents a new data gathering algorithm called Connectivity-Based Data Collection (CBDC). The CBDC algorithm utilizes the connectivity between sensor nodes so as to determine the trajectory of the mobile sink whilst satisfying its path constraint and minimizing the number of multi-hop communications. The presented results show that CBDC, in comparison with the LEACH-C algorithm, prolongs the network life time at different connectivity levels of sensor networks, varying number of sensor nodes and at different path constraints of the mobile sink.展开更多
基金support from the National Natural Science Foundation of China(39670436)Young Scientists’ Fund of National Natu-ral Science Foundation of China (30600075)Young Scientific Cadremen’s Fund of Northwest A&F University, China
文摘XN901 is a K-type three-line hybrid wheat with a high yield potential, and its leaves and stem remaining green during grain maturation, suggesting much assimilate stay in leaves and stem. The grain water content, grain volume, carbohydrate content, and enzyme activity of sucose metabolism in the grain, as well as source-sink relationship were studied in order to investigate the physiological reason of the assimilate remaining in leaves and stem at the late stage. The results showed that the hybrid grains had more water and soluble sugar, higher activities of acid invertase and sucrose synthase at the early stage that led to a faster expansion growth, greater grain volume and faster starch synthesis at the early to mid stage of grain development. Also it had a longer period for actively filling. As a result, the grain weight and yield of the hybrid were increased by 14 and 15% respectively compared to that of Shaan 229. Additionally, the biomass of XN901 was 41.7% more than that of control, but its harvest index was 9% lower than Shaan 229. However, its lower activity of sucrose synthase indicated a lower sink activity at the late stage, resulting in a slow rate of filling and starch synthesis. Also, the hybrid wheat XN901 had a large source-sink ratio. It is the main reason for much assimilate remaining in the straw at the late stage and lower harvest index. Strengthening the sink activity and raising the harvest index should be the key means of improving the yield of hybrid wheat.
基金supported by the National Natural Science Foundation of China (31871567)the National Key Research and Development Program of China (2017YFD0300202-2)Tang Young Scholar (2017)。
文摘Poor filling and low weight of inferior kernels limit the further improvement of wheat yield. Two cultivars, Shuangda 1 and Xinong 538, with different grain weights, were selected to investigate the physiological changes of inferior kernels by removal of superior kernels(RS) at the flowering stage. i TRAQ combined with physiological indexes was used to identify factors limiting the filling of inferior kernels.Removal of superior kernels significantly increased the mean filling rate of inferior kernels and thus increased their weight. A set of 6012 proteins in inferior wheat kernels were differentially expressed between the RS and control. These differentially expressed proteins were involved mainly in carbon metabolism and energy metabolism. The main reason for the promoting effect of RS on the filling of inferior kernels may be that RS downregulated proteins involved in glycolysis and pyruvate metabolism while upregulating proteins involved in carbon fixation and photosynthesis. Consequently, RS greatly increased the ATP content in inferior kernels, supplying energy for them to absorb photosynthetic assimilates. Removal of superior kernels increased the activities of sucrose synthase, soluble starch synthase,adenosine diphosphate glucose pyrophosphorylase, and starch branching enzyme in inferior kernels and promoted starch accumulation in them. Thus, RS promoted the filling of inferior kernels and increased their weight. The promoting effect of RS on starch synthesis in inferior kernels was associated with their endogenous IAA and ABA levels.
基金supported by the National Natural Science Foundation of China(31672128)the Chinese Universities Scientific Fund(2452020007)。
文摘Auxin(indole-3-acetic acid, IAA) has a considerable impact on the regulation of plant carbohydrate levels and growth, but the mechanism by which it regulates sugar levels in plants has received little attention. In this study, we found that exogenous IAA altered fructose(Fru), glucose(Glc), and sucrose(Suc) concentrations in shoot tips mainly by regulating MdSUSY1, MdFRK2, MdHxK1 and MdSDH2 transcript levels. Additionally, we used 5-year-old ’Royal Gala’ apple trees to further verify that these genes play primary roles in regulating sink strength. The results showed that MdSUSY1, MdFRK2, MdHxK1/3 and MdSDH2 might be major contributors to sink strength regulation. Taken together, these results provide new insight into the regulation of the carbohydrate metabolism mechanism, which will be helpful for regulating sink strength and yield.
文摘青藏高原是中国陆地生态系统的一个重要的碳汇功能区,但其碳汇强度及潜力的估算存在很大的差异,不利于区域碳中和目标的实现。基于涡度相关技术观测的原生高寒草地生态系统CO_(2)通量的40个站点年数据,结合2000年~2018年的年均气温、年均降水和年最大归一化植被指数(NDVIm)等因子,构建增强回归树模型以研究青海省陆地生态系统碳汇潜力及空间特征。结果表明增强回归树模型能够较好地模拟原生高寒草地碳汇强度的时空变异(R2=0.61),碳汇强度的观测值与模拟值的均方根误差和平均绝对误差分别为33.78 g C/m^(2)和26.63 g C/m^(2)。年均气温和NDVIm是高寒草地碳汇强度时空变异的主要影响因子,二者的相对贡献分别为48.6%和39.0%。青海省陆地生态系统每年的碳汇潜力平均为44.82±22.57 g C/m^(2)(平均值±标准差),高值区集中在海北州的中部及黄南州、果洛州和玉树州的东南部,低值区分布在海西州、海南州和海东市。青海省陆地生态系统每年的碳汇潜力总和为16.60 Mt C,其中高寒草甸和高寒草原分别为11.48 Mt C和3.13 Mt C,是青海省碳汇功能维持和提升的重点保育对象。研究结果可为青海省陆地生态系统的功能评估及率先实现碳中和目标提供数据支撑。
文摘The design of an effective and robust data gathering algorithm is crucial to the overall performance of wireless sensor networks (WSN). However, using traditional routing algorithms for data gathering is energy-inefficient for sensor nodes with limited power resources and multi-hop communication protocols. Data gathering with mobile sinks provided an effective solution to this problem. The major drawback of this approach is the time and path constraints of the mobile sink, which limit the mobile sink to collect data from all sensor nodes and, then, data routing is still required for these unreachable parts by the mobile sink. This paper presents a new data gathering algorithm called Connectivity-Based Data Collection (CBDC). The CBDC algorithm utilizes the connectivity between sensor nodes so as to determine the trajectory of the mobile sink whilst satisfying its path constraint and minimizing the number of multi-hop communications. The presented results show that CBDC, in comparison with the LEACH-C algorithm, prolongs the network life time at different connectivity levels of sensor networks, varying number of sensor nodes and at different path constraints of the mobile sink.
