Abstract：Aim To investigate the effect of hydrogen-rich saline on arterial thrombosis in sinoaortic denervatted （SAD） rats. Methods Rats were divided randomly into Sham group, SAD group, SAD ＋ hydrogen-rich saline...Abstract：Aim To investigate the effect of hydrogen-rich saline on arterial thrombosis in sinoaortic denervatted （SAD） rats. Methods Rats were divided randomly into Sham group, SAD group, SAD ＋ hydrogen-rich saline （5 mL · kg^-1 · d^-1） group and SAD ＋ hydrogen-rich saline （10 mL · kg^-1 · d^-1） group. Four weeks after Sham or SAD operation, rats were given intraperitoneally normal saline or hydrogen-rich saline for 4 weeks. Carotid artery thrombosis was induced by 30% ferric chloride. Time till occlusion was measured by Laser Doppler Flowme- try. Results When compared with the Sham group, time till occlusion was significantly shorter in SAD group. Moreover, platelet aggregation, platelet adhesion to collagen and the level of ROS and malondialdehyde （MDA） in plasma and Ca2＋ in platelets were significantly raised. The level of NO and SOD in plasma and NO in platelet were significantly reduced. When compared with the SAD group, time till occlusion in hydrogen-rich saline group group was significantly prolonged. Platelet aggregation, adhesion, the level of ROS, MDA and Ca2＋ were significantly re- duced, whereas the level of NO and SOD in plasma and NO in platelets were significantly higher. Conclusion Hydrogen-rich saline might improve arterial thrombosis in SAD rats through inhibition of platelet activation and oxi- dative stress.展开更多
Background and Objective:Increased blood pressure variability(BPV),which has been considered to cause brain damage,can be induced by sinoaortic denervation(SAD)in rats.This study was designed to test the hypothesis th...Background and Objective:Increased blood pressure variability(BPV),which has been considered to cause brain damage,can be induced by sinoaortic denervation(SAD)in rats.This study was designed to test the hypothesis that increased BPV impairs learning and memory in rats with SAD.Methods:SAD was performed in male Sprague-Dawley rats.Passive avoidance trial was used to evaluate learning and memory ability.Results:Compared with shamoperated(Sham)group,there was no significant difference in the latency of passive avoidance in adaption trial.The latency of avoiding darkness in retention trial in SAD group was significantly lower than that in Sham group both 2 and 16 weeks after SAD(P<0.05,P<0.01).Westernblot assay revealed that all the expression of choline acetyltransferase,vesicular acetylcholine transporter andα7 nicotinic acetylcholine receptor decreaed in both cerebral cortex(P<0.05)and hippocampus(P<0.05)16 weeks after SAD(P<0.05),while only level ofα7 nicotinic acetylcholine receptor was reduced in hippocampus 2 weeks after SAD(P<0.05).Conclusion:Increasd BPV reduces memory ability in SAD rats,potentially through cholinergic pathway.展开更多
文摘Abstract：Aim To investigate the effect of hydrogen-rich saline on arterial thrombosis in sinoaortic denervatted （SAD） rats. Methods Rats were divided randomly into Sham group, SAD group, SAD ＋ hydrogen-rich saline （5 mL · kg^-1 · d^-1） group and SAD ＋ hydrogen-rich saline （10 mL · kg^-1 · d^-1） group. Four weeks after Sham or SAD operation, rats were given intraperitoneally normal saline or hydrogen-rich saline for 4 weeks. Carotid artery thrombosis was induced by 30% ferric chloride. Time till occlusion was measured by Laser Doppler Flowme- try. Results When compared with the Sham group, time till occlusion was significantly shorter in SAD group. Moreover, platelet aggregation, platelet adhesion to collagen and the level of ROS and malondialdehyde （MDA） in plasma and Ca2＋ in platelets were significantly raised. The level of NO and SOD in plasma and NO in platelet were significantly reduced. When compared with the SAD group, time till occlusion in hydrogen-rich saline group group was significantly prolonged. Platelet aggregation, adhesion, the level of ROS, MDA and Ca2＋ were significantly re- duced, whereas the level of NO and SOD in plasma and NO in platelets were significantly higher. Conclusion Hydrogen-rich saline might improve arterial thrombosis in SAD rats through inhibition of platelet activation and oxi- dative stress.
基金This work was supported by the Natural Science Foundation of Shandong Province of China(ZR2014HQ007,ZR2017MH048),the Foundation of Overseas Distinguished Taishan Scholars of Shandong Province of China(to H-TZ)and Collaborative Innovation Center for Research and Development of Traditional Chinese Medicine in Mount Tai.
文摘Background and Objective:Increased blood pressure variability(BPV),which has been considered to cause brain damage,can be induced by sinoaortic denervation(SAD)in rats.This study was designed to test the hypothesis that increased BPV impairs learning and memory in rats with SAD.Methods:SAD was performed in male Sprague-Dawley rats.Passive avoidance trial was used to evaluate learning and memory ability.Results:Compared with shamoperated(Sham)group,there was no significant difference in the latency of passive avoidance in adaption trial.The latency of avoiding darkness in retention trial in SAD group was significantly lower than that in Sham group both 2 and 16 weeks after SAD(P<0.05,P<0.01).Westernblot assay revealed that all the expression of choline acetyltransferase,vesicular acetylcholine transporter andα7 nicotinic acetylcholine receptor decreaed in both cerebral cortex(P<0.05)and hippocampus(P<0.05)16 weeks after SAD(P<0.05),while only level ofα7 nicotinic acetylcholine receptor was reduced in hippocampus 2 weeks after SAD(P<0.05).Conclusion:Increasd BPV reduces memory ability in SAD rats,potentially through cholinergic pathway.
