Objective To determine the in vitro possible clastogenic and cytotoxic activities of Ulva rigida crude extracts （URE）, and identify their antigenotoxic and protective effects on chemotherapeutic agent mitomycine-C ...Objective To determine the in vitro possible clastogenic and cytotoxic activities of Ulva rigida crude extracts （URE）, and identify their antigenotoxic and protective effects on chemotherapeutic agent mitomycine-C （MMC）. Methods Anti-clastogenic and anti-genotoxic activities of Ulva rigida crude extracts （URE） were studied using chromosome aberration （CA）, sister chromatid exchange （SCE）, and micronuclei （MN） tests in human lymphocytes cultured in vitro. Results The chromosome aberration, sister chromatid exchange or micronuclei tests showed that URE at concentrations of 10, 20, and 40 lag/mL had no clastogenic activity in human lymphocyte cell culture. Three doses of URE significantly decreased the number of chromosomal aberrations and the frequencies of SCE and MN when compared with the culture treated with MMC （P〈0.0001）. Conclusion Although URE itself is not a clastogenic or cytotoxic substance, it possesses strong antigenotoxic, anti-clastogenic, and protective effects on MMC in vitro.展开更多
The air-dust samples collected from petro-chemical industrial region in the suburb of Lanzhou and from a certain rural region 64 km away from the city were extracted, with a mixed solvent (benzene: hexane: isopropanol...The air-dust samples collected from petro-chemical industrial region in the suburb of Lanzhou and from a certain rural region 64 km away from the city were extracted, with a mixed solvent (benzene: hexane: isopropanol=7:2:1) for 8 hours. A strong free radical signal at g= 2.00 of air-dust itself and a hyperfine splitting EPR signal of extract from air-dust have been detected. The sister chromatid exchange frequency (SCE) was increased by extracts of both dusts from the industrial region and from the rural region. If a chemical is able to increase SCE up to twice as high as the control, this chemical is considered to be mutagenic and/or carcinogenic. The double SCE frequency concentration is 23 μg/ml for the dust extract obtained from the industrial region and 47μg/ml for that from the rural region. Extracts were able to damage to DNA template. Results indicated that the mutagenicity and/or carcinogenicity of the extracts obtained from the petro-chemical industrial region were stronger than that of the extracts from the rural region.展开更多
目的:Siloranes是一种含硅的新型牙科复合树脂环氧乙烷单体,较传统的含碳的环氧乙烷单体具有更好的生物相容性。本研究的目的为用哺乳动物细胞培养检测姐妹染色体交换的方法评估Siloranes的基因毒性,并与传统的环氧乙烷比较。方法:研究...目的:Siloranes是一种含硅的新型牙科复合树脂环氧乙烷单体,较传统的含碳的环氧乙烷单体具有更好的生物相容性。本研究的目的为用哺乳动物细胞培养检测姐妹染色体交换的方法评估Siloranes的基因毒性,并与传统的环氧乙烷比较。方法:研究所用的传统环氧乙烷为CyracureTM UVR6105。Silorane包括Ph-Sil,TET-Sil和二者50/50(w/w)的混合物(3M-ESPE)。用CHO细胞在有或无S9情况下检测姐妹染色体交换(SCE)。Bonferron post hoc统计分析检测有无显著性差异。结果:与溶剂对照相比,在有或无S9情况下,环氧乙烷UVR6105显著性地增加了培养CHO细胞的SCE数目。3种Silorane单体Ph-Sil,TET-Sil或Sil-Mix均未能增加SCE数目。结论:用哺乳动物细胞培养检测姐妹染色体交换的方法评估显示,3种新型Silorane单体Ph-Sil,TET-Sil或Sil-Mix均未检测出基因毒性效果,提示Silorane单体具有较低基因毒性,是适于研发牙科树脂的新型单体。展开更多
Protein phosphatase 2A(PP2A)plays a critical multi-faceted role in the regulation of the cell cycle.PP2A is involved in such diverse processes by the formation of structurally distinct families of holoenzymes,which ar...Protein phosphatase 2A(PP2A)plays a critical multi-faceted role in the regulation of the cell cycle.PP2A is involved in such diverse processes by the formation of structurally distinct families of holoenzymes,which are regulated spatially and temporally by specific regulators.Mitosis requires the correct arrangement of sister chromatids so that replicated chromosomes can bind correctly to microtubules and segregate towards opposite poles.A large number of studies have shown that PP2A is mainly involved in a series of phosphorylation processes during the G2/M phase transition and the termination of M phase.Moreover,PP2A shows a more substantial contribution to sister chromatid cohesion and microtubule-kinetochore attachment.These processes are all crucial for proper cell survival and proliferation and are often deregulated in cancer and other diseases.展开更多
Background: In oral and maxillofacial surgery, synthetic bone grafts are most widely used as bone substitutes, due to the limited sources of autologous bone. The aim of this study was to examine the influence of three...Background: In oral and maxillofacial surgery, synthetic bone grafts are most widely used as bone substitutes, due to the limited sources of autologous bone. The aim of this study was to examine the influence of three different synthetic bone grafts (Cerasorb, Fortoss and Perioglass) on sisters chromatide exchanges (SCEs) in peripheral lymphocytes. Materials and Methods: Peripheral blood samples taken from 68 patients (45 females and 23 males), who underwent oral surgery procedures, such as apical resection, cyst enucleation or periodontal curretage, were obtained for SCE a day before and two months after the surgeries. A control group included 30 patients, while the study group was made of the patients who underwent bone grafting with Cerasorb? (11 patients), Fortoss? VITAL (10 patients) or Perioglass? (17 patients). Results: Comparing with the results of the study group before and after the treatment, it was concluded that the results were statistically significant (p = 0.001). In the Perioglass? subgroup, a greater statistical significance (p = 0.003) was noted, than that in either the Cerasorb? (p = 0.620) or Fortoss? (p = 0.210) subgroups, in which there was no statistical significance. Conclusions: Although further investigations may be necessary, our results suggest that the synthetic bone grafts might have an influence on SCE in peripheral lymphocytes.展开更多
基金supported by a grant from the Scientific and Technical Research Council of Turkey, Ankara (TüBiTAK, 107T279 (TBAG/HD-304).
文摘Objective To determine the in vitro possible clastogenic and cytotoxic activities of Ulva rigida crude extracts （URE）, and identify their antigenotoxic and protective effects on chemotherapeutic agent mitomycine-C （MMC）. Methods Anti-clastogenic and anti-genotoxic activities of Ulva rigida crude extracts （URE） were studied using chromosome aberration （CA）, sister chromatid exchange （SCE）, and micronuclei （MN） tests in human lymphocytes cultured in vitro. Results The chromosome aberration, sister chromatid exchange or micronuclei tests showed that URE at concentrations of 10, 20, and 40 lag/mL had no clastogenic activity in human lymphocyte cell culture. Three doses of URE significantly decreased the number of chromosomal aberrations and the frequencies of SCE and MN when compared with the culture treated with MMC （P〈0.0001）. Conclusion Although URE itself is not a clastogenic or cytotoxic substance, it possesses strong antigenotoxic, anti-clastogenic, and protective effects on MMC in vitro.
基金This project was supported by National Natural Science Foundation of China.
文摘The air-dust samples collected from petro-chemical industrial region in the suburb of Lanzhou and from a certain rural region 64 km away from the city were extracted, with a mixed solvent (benzene: hexane: isopropanol=7:2:1) for 8 hours. A strong free radical signal at g= 2.00 of air-dust itself and a hyperfine splitting EPR signal of extract from air-dust have been detected. The sister chromatid exchange frequency (SCE) was increased by extracts of both dusts from the industrial region and from the rural region. If a chemical is able to increase SCE up to twice as high as the control, this chemical is considered to be mutagenic and/or carcinogenic. The double SCE frequency concentration is 23 μg/ml for the dust extract obtained from the industrial region and 47μg/ml for that from the rural region. Extracts were able to damage to DNA template. Results indicated that the mutagenicity and/or carcinogenicity of the extracts obtained from the petro-chemical industrial region were stronger than that of the extracts from the rural region.
文摘目的:Siloranes是一种含硅的新型牙科复合树脂环氧乙烷单体,较传统的含碳的环氧乙烷单体具有更好的生物相容性。本研究的目的为用哺乳动物细胞培养检测姐妹染色体交换的方法评估Siloranes的基因毒性,并与传统的环氧乙烷比较。方法:研究所用的传统环氧乙烷为CyracureTM UVR6105。Silorane包括Ph-Sil,TET-Sil和二者50/50(w/w)的混合物(3M-ESPE)。用CHO细胞在有或无S9情况下检测姐妹染色体交换(SCE)。Bonferron post hoc统计分析检测有无显著性差异。结果:与溶剂对照相比,在有或无S9情况下,环氧乙烷UVR6105显著性地增加了培养CHO细胞的SCE数目。3种Silorane单体Ph-Sil,TET-Sil或Sil-Mix均未能增加SCE数目。结论:用哺乳动物细胞培养检测姐妹染色体交换的方法评估显示,3种新型Silorane单体Ph-Sil,TET-Sil或Sil-Mix均未检测出基因毒性效果,提示Silorane单体具有较低基因毒性,是适于研发牙科树脂的新型单体。
文摘Protein phosphatase 2A(PP2A)plays a critical multi-faceted role in the regulation of the cell cycle.PP2A is involved in such diverse processes by the formation of structurally distinct families of holoenzymes,which are regulated spatially and temporally by specific regulators.Mitosis requires the correct arrangement of sister chromatids so that replicated chromosomes can bind correctly to microtubules and segregate towards opposite poles.A large number of studies have shown that PP2A is mainly involved in a series of phosphorylation processes during the G2/M phase transition and the termination of M phase.Moreover,PP2A shows a more substantial contribution to sister chromatid cohesion and microtubule-kinetochore attachment.These processes are all crucial for proper cell survival and proliferation and are often deregulated in cancer and other diseases.
文摘Background: In oral and maxillofacial surgery, synthetic bone grafts are most widely used as bone substitutes, due to the limited sources of autologous bone. The aim of this study was to examine the influence of three different synthetic bone grafts (Cerasorb, Fortoss and Perioglass) on sisters chromatide exchanges (SCEs) in peripheral lymphocytes. Materials and Methods: Peripheral blood samples taken from 68 patients (45 females and 23 males), who underwent oral surgery procedures, such as apical resection, cyst enucleation or periodontal curretage, were obtained for SCE a day before and two months after the surgeries. A control group included 30 patients, while the study group was made of the patients who underwent bone grafting with Cerasorb? (11 patients), Fortoss? VITAL (10 patients) or Perioglass? (17 patients). Results: Comparing with the results of the study group before and after the treatment, it was concluded that the results were statistically significant (p = 0.001). In the Perioglass? subgroup, a greater statistical significance (p = 0.003) was noted, than that in either the Cerasorb? (p = 0.620) or Fortoss? (p = 0.210) subgroups, in which there was no statistical significance. Conclusions: Although further investigations may be necessary, our results suggest that the synthetic bone grafts might have an influence on SCE in peripheral lymphocytes.
