Two phenylethanoid glycosides, acteoside and forsythoside B, were first isolated from the traditional Chinese herb Callicarpa peii H.T. Chang. The interaction between the two phenylethanoid glycosides and bovine serum...Two phenylethanoid glycosides, acteoside and forsythoside B, were first isolated from the traditional Chinese herb Callicarpa peii H.T. Chang. The interaction between the two phenylethanoid glycosides and bovine serum albumin （BSA） was investigated by fluorescence, UV vis absorbance and circular dichroism （CD）. The results showed that the quenching mechanism in the drug-BSA binary systems was a combination of static quenching and non- radiative energy transfer. Displacement experiments confirmed that the drug bound to the site I of BSA. UVvis and CD measurements indicated that the binding of the drug to BSA induced conformational changes in BSA.展开更多
基金the National NaturalScience Foundation of China (Grant nos.81173535 and 81001613)
文摘Two phenylethanoid glycosides, acteoside and forsythoside B, were first isolated from the traditional Chinese herb Callicarpa peii H.T. Chang. The interaction between the two phenylethanoid glycosides and bovine serum albumin （BSA） was investigated by fluorescence, UV vis absorbance and circular dichroism （CD）. The results showed that the quenching mechanism in the drug-BSA binary systems was a combination of static quenching and non- radiative energy transfer. Displacement experiments confirmed that the drug bound to the site I of BSA. UVvis and CD measurements indicated that the binding of the drug to BSA induced conformational changes in BSA.
