The CDTF-Library is a library in Chinese-German Technical Faculty of Qingdao University of Science and Technology in Qingdao.It is very small in scale.It only occupies an area of 60 square meters with 3 reading seats ...The CDTF-Library is a library in Chinese-German Technical Faculty of Qingdao University of Science and Technology in Qingdao.It is very small in scale.It only occupies an area of 60 square meters with 3 reading seats and 5000 original German books.The resources of German in domestic are relatively few and German resources are too expensive to buy.During the past years,more and more readers made complaints,against the limited resource of audio and video materials,lack of new books,short open time and unsatisfied technical environments.展开更多
Seashore landfill aquifers are environments of special physicochemical conditions (high organic load and high sa- linity), and microbes in leachate-polluted aquifers play a significant role for intrinsic bioremediatio...Seashore landfill aquifers are environments of special physicochemical conditions (high organic load and high sa- linity), and microbes in leachate-polluted aquifers play a significant role for intrinsic bioremediation. In order to characterize microbial diversity and look for clues on the relationship between microbial community structure and hydrochemistry, a cul- ture-independent examination of a typical groundwater sample obtained from a seashore landfill was conducted by sequence analysis of 16S rDNA clone library. Two sets of universal 16S rDNA primers were used to amplify DNA extracted from the groundwater so that problems arising from primer efficiency and specificity could be reduced. Of 74 clones randomly selected from the libraries, 30 contained unique sequences whose analysis showed that the majority of them belonged to bacteria (95.9%), with Proteobacteria (63.5%) being the dominant division. One archaeal sequence and one eukaryotic sequence were found as well. Bacterial sequences belonging to the following phylogenic groups were identified: Bacteroidetes (20.3%), β, γ, δ and ε-subdivisions of Proteobacteria (47.3%, 9.5%, 5.4% and 1.3%, respectively), Firmicutes (1.4%), Actinobacteria (2.7%), Cyanobacteria (2.7%). The percentages of Proteobacteria and Bacteroides in seawater were greater than those in the groundwater from a non-seashore landfill, indicating a possible influence of seawater. Quite a few sequences had close relatives in marine or hypersaline environments. Many sequences showed affiliations with microbes involved in anaerobic fermentation. The remarkable abundance of sequences related to (per)chlorate-reducing bacteria (ClRB) in the groundwater was significant and worthy of further study.展开更多
AIM: To develop and optimize cDNA representational difference analysis (cDNA RDA) method and to identify and clone garlic up-regulated genes in human gastric cancer (HGC) cells. METHODS: We performed cDNA RDA method b...AIM: To develop and optimize cDNA representational difference analysis (cDNA RDA) method and to identify and clone garlic up-regulated genes in human gastric cancer (HGC) cells. METHODS: We performed cDNA RDA method by using abundant double-stranded cDNA messages provided by two self-constructed cDNA libraries (Allitridi-treated and paternal HGC cell line BGC823 cells cDNA libraries respectively). Bam H I and Xho I restriction sites harbored in the library vector were used to select representations. Northern and Slot blots analyses were employed to identify the obtained difference products. RESULTS: Fragments released from the cDNA library vector after restriction endonuclease digestion acted as good marker indicating the appropriate digestion degree for library DNA. Two novel expressed sequence tags (ESTs) and a recombinant gene were obtained. Slot blots result showed a 8-fold increase of glia-derived nexin/protease nexin 1 (GDN/PN1) gene expression level and 4-fold increase of hepatitis B virus x-interacting protein (XIP) mRNA level in BGC823 cells after Allitridi treatment for 72h. CONCLUSION: Elevated levels of GDN/PN1 and XIP mRNAs induced by Allitridi provide valuable molecular evidence for elucidating the garlic's efficacies against neurodegenerative and inflammatory diseases. Isolation of a recombinant gene and two novel ESTs further show cDNA RDA based on cDNA libraries to be a powerful method with high specificity and reproducibility in cloning differentially expressed genes.展开更多
目的:以SCI数据库文献为例,探讨建立B/S模式(Browser/Server,浏览器/服务器模式)医学文献分析系统的可行性。方法:采用B/S模式下的LAMP技术架构设计文献分析系统,将处理结果使用百度ECharts可视化工具输出或以EndNote格式导出。结果:系...目的:以SCI数据库文献为例,探讨建立B/S模式(Browser/Server,浏览器/服务器模式)医学文献分析系统的可行性。方法:采用B/S模式下的LAMP技术架构设计文献分析系统,将处理结果使用百度ECharts可视化工具输出或以EndNote格式导出。结果:系统可以将SCI(Web of Science平台)数据库下载的文献批量处理,输出可视化结果,用于辅助医学科研文献阅读。结论:建立与数字图书馆技术路线相同的文献分析系统,可与医学数字图书馆同时进行软硬件维护,降低了维护成本,并为进一步研究B/S模式知识发现系统做好技术储备。展开更多
文摘The CDTF-Library is a library in Chinese-German Technical Faculty of Qingdao University of Science and Technology in Qingdao.It is very small in scale.It only occupies an area of 60 square meters with 3 reading seats and 5000 original German books.The resources of German in domestic are relatively few and German resources are too expensive to buy.During the past years,more and more readers made complaints,against the limited resource of audio and video materials,lack of new books,short open time and unsatisfied technical environments.
基金Project (No. 20377030) supported by the National Natural ScienceFoundation of China
文摘Seashore landfill aquifers are environments of special physicochemical conditions (high organic load and high sa- linity), and microbes in leachate-polluted aquifers play a significant role for intrinsic bioremediation. In order to characterize microbial diversity and look for clues on the relationship between microbial community structure and hydrochemistry, a cul- ture-independent examination of a typical groundwater sample obtained from a seashore landfill was conducted by sequence analysis of 16S rDNA clone library. Two sets of universal 16S rDNA primers were used to amplify DNA extracted from the groundwater so that problems arising from primer efficiency and specificity could be reduced. Of 74 clones randomly selected from the libraries, 30 contained unique sequences whose analysis showed that the majority of them belonged to bacteria (95.9%), with Proteobacteria (63.5%) being the dominant division. One archaeal sequence and one eukaryotic sequence were found as well. Bacterial sequences belonging to the following phylogenic groups were identified: Bacteroidetes (20.3%), β, γ, δ and ε-subdivisions of Proteobacteria (47.3%, 9.5%, 5.4% and 1.3%, respectively), Firmicutes (1.4%), Actinobacteria (2.7%), Cyanobacteria (2.7%). The percentages of Proteobacteria and Bacteroides in seawater were greater than those in the groundwater from a non-seashore landfill, indicating a possible influence of seawater. Quite a few sequences had close relatives in marine or hypersaline environments. Many sequences showed affiliations with microbes involved in anaerobic fermentation. The remarkable abundance of sequences related to (per)chlorate-reducing bacteria (ClRB) in the groundwater was significant and worthy of further study.
基金the Natural Scientific Foundation of China (NSFC3962526)National High-Technology Project-863 (102-10-01-04)
文摘AIM: To develop and optimize cDNA representational difference analysis (cDNA RDA) method and to identify and clone garlic up-regulated genes in human gastric cancer (HGC) cells. METHODS: We performed cDNA RDA method by using abundant double-stranded cDNA messages provided by two self-constructed cDNA libraries (Allitridi-treated and paternal HGC cell line BGC823 cells cDNA libraries respectively). Bam H I and Xho I restriction sites harbored in the library vector were used to select representations. Northern and Slot blots analyses were employed to identify the obtained difference products. RESULTS: Fragments released from the cDNA library vector after restriction endonuclease digestion acted as good marker indicating the appropriate digestion degree for library DNA. Two novel expressed sequence tags (ESTs) and a recombinant gene were obtained. Slot blots result showed a 8-fold increase of glia-derived nexin/protease nexin 1 (GDN/PN1) gene expression level and 4-fold increase of hepatitis B virus x-interacting protein (XIP) mRNA level in BGC823 cells after Allitridi treatment for 72h. CONCLUSION: Elevated levels of GDN/PN1 and XIP mRNAs induced by Allitridi provide valuable molecular evidence for elucidating the garlic's efficacies against neurodegenerative and inflammatory diseases. Isolation of a recombinant gene and two novel ESTs further show cDNA RDA based on cDNA libraries to be a powerful method with high specificity and reproducibility in cloning differentially expressed genes.
文摘目的:以SCI数据库文献为例,探讨建立B/S模式(Browser/Server,浏览器/服务器模式)医学文献分析系统的可行性。方法:采用B/S模式下的LAMP技术架构设计文献分析系统,将处理结果使用百度ECharts可视化工具输出或以EndNote格式导出。结果:系统可以将SCI(Web of Science平台)数据库下载的文献批量处理,输出可视化结果,用于辅助医学科研文献阅读。结论:建立与数字图书馆技术路线相同的文献分析系统,可与医学数字图书馆同时进行软硬件维护,降低了维护成本,并为进一步研究B/S模式知识发现系统做好技术储备。
