根据烟草Nt-syr1基因mRNA序列设计特异引物,建立了SYBR Green I实时荧光定量PCR反应体系,对烟株打顶后叶片Nt-syr1基因进行了mRNA转录水平上的定量分析,为从分子生物学水平上研究烤烟钾素营养调控机理提供新的技术手段。该方法简单实用...根据烟草Nt-syr1基因mRNA序列设计特异引物,建立了SYBR Green I实时荧光定量PCR反应体系,对烟株打顶后叶片Nt-syr1基因进行了mRNA转录水平上的定量分析,为从分子生物学水平上研究烤烟钾素营养调控机理提供新的技术手段。该方法简单实用,获得的荧光定量PCR扩增曲线基线平整,指数区扩增明显,斜率大;稳定性和重现性好,变异系数小;循环阈值Ct与PCR起始模板量的对数值之间存在良好的线性关系。对基因表达结果分析表明,烟株打顶后1 h,Nt-syr1基因在叶片中强烈表达,表达量约是同期不打顶处理的480倍,随后逐渐降低。与打顶处理相比,打顶后涂抹生长调节剂可以降低其表达量。展开更多
The effects of human activities on the soil cover transformation in the eastern part of Kazakhstan were investigated during the period of 1956-2008.The results of the research for different soil types in Priaralye ind...The effects of human activities on the soil cover transformation in the eastern part of Kazakhstan were investigated during the period of 1956-2008.The results of the research for different soil types in Priaralye indicated that there was 643.3×103 hm2 solonchaks,accounting for 38.5 % of the total area（1670.5×10^3 hm^2） in 2008.Vast areas are occupied with dried lakeshore soil（311.1× 10^3 hm^2）,sandy soils（147.6×10^3 hm^2） and grey-brown desert soils and solonetzes（146.7×10^3 hm^2）.In 2001 the area of solonchak was 755×103 hm2 and decreased to 643.3×10^3 hm^2 in 2008,which due to the shrinkage of the Aral Sea,the areas of marsh and lakeshore solonchaks decreased with the increase of dried bottom of the Aral Sea.The level of soil cover transformation in the modern delta of the Syr-Darya River can be seen from the comparison of the results obtained from the different years in the study area.The area of solonchaks increased by 10×10^3 hm^2 and the area of alluvial-meadow salinizied soils increased by 17.9×10^3 hm^2 during the period of 1956-1969.It means that many non-salinizied soils were transformed into salinizied ones.Striking changes occurred in the structure of soil cover as a result of aridization.So,the researches in1969 significantly determined the areas of hydromorphic soils subjected to desertification（it was not fixed on the map before 1956）.Later,these soils were transformed into takyr-like soils.The area of takyr-like soils increased almost by 3 times for 34 years（from 1956 to 1990）.The long-term soil researches on soil cover transformation in Priaralye have shown that the tendencies of negative processes（salinization and deflation） are being kept and lead to further soil and eco-environment degradation in the region.展开更多
The shrinkage of the Aral Sea,which is closely related to the Amu Darya River,strongly affects the sustainability of the local natural ecosystem,agricultural production,and human well-being.In this study,we used the B...The shrinkage of the Aral Sea,which is closely related to the Amu Darya River,strongly affects the sustainability of the local natural ecosystem,agricultural production,and human well-being.In this study,we used the Bayesian Estimator of Abrupt change,Seasonal change,and Trend(BEAST)model to detect the historical change points in the variation of the Aral Sea and the Amu Darya River and analyse the causes of the Aral Sea shrinkage during the 1950–2016 period.Further,we applied multifractal detrend cross-correlation analysis(MF-DCCA)and quantitative analysis to investigate the responses of the Aral Sea to the runoff in the Amu Darya River,which is the main source of recharge to the Aral Sea.Our results showed that two significant trend change points in the water volume change of the Aral Sea occurred,in 1961 and 1974.Before 1961,the water volume in the Aral Sea was stable,after which it began to shrink,with a shrinkage rate fluctuating around 15.21 km3/a.After 1974,the water volume of the Aral Sea decreased substantially at a rate of up to 48.97 km3/a,which was the highest value recorded in this study.In addition,although the response of the Aral Sea's water volume to its recharge runoff demonstrated a complex non-linear relationship,the replenishment of the Aral Sea by the runoff in the lower reaches of the Amu Darya River was identified as the dominant factor affecting the Aral Sea shrinkage.Based on the scenario analyses,we concluded that it is possible to slow down the retreat of the Aral Sea and restore its ecosystem by increasing the efficiency of agricultural water use,decreasing agricultural water use in the middle and lower reaches,reducing ineffective evaporation from reservoirs and wetlands,and increasing the water coming from the lower reaches of the Amu Darya River to the 1961–1973 level.These measures would maintain and stabilise the water area and water volume of the Aral Sea in a state of ecological restoration.Therefore,this study focuses on how human consumption of recharge runoff affects the Aral Sea and provides scientific perspective on its ecological conservation and sustainable development.展开更多
Systemin, the first peptide hormone identified in plants, was initially isolated from tomato(Solanum lycopersicum) leaves. Systemin mediates local and systemic wound-induced defense responses in plants, conferring res...Systemin, the first peptide hormone identified in plants, was initially isolated from tomato(Solanum lycopersicum) leaves. Systemin mediates local and systemic wound-induced defense responses in plants, conferring resistance to necrotrophic fungi and herbivorous insects. Systemin is recognized by the leucine-rich-repeat receptor-like kinase(LRRRLK) receptor SYSTEMIN RECEPTOR1(SYR1), but how the systemin recognition signal is transduced to intracellular signaling pathways to trigger defense responses is poorly understood. Here, we demonstrate that SERK family LRR-RLKs function as coreceptors for SYR1 to mediate systemin signal transduction in tomato. By using chemical genetic approaches coupled with engineered receptors, we revealed that the association of the cytoplasmic kinase domains of SYR1 with SERKs leads to their mutual trans-phosphorylation and the activation of SYR1, which in turn induces a wide range of defense responses. Systemin stimulates the association between SYR1 and all tomato SERKs(SlSERK1,SlSERK3A, and SlSERK3B). The resulting SYR1-SlSERK heteromeric complexes trigger the phosphorylation of TOMATO PROTEIN KINASE 1B(TPK1b), a receptor-like cytoplasmic kinase that positively regulates systemin responses. Additionally,upon association with SYR1, SlSERKs are cleaved by the Pseudomonas syringae effector HopB1, further supporting the finding that SlSERKs are activated by systemin-bound SYR1. Finally, genetic analysis using Slserk mutants showed that SlSERKs are essential for systemin-mediated defense responses. Collectively, these findings demonstrate that the systeminmediated association of SYR1 and SlSERKs activates defense responses against herbivorous insects.展开更多
咸海周边环境变化对中亚干旱区生态稳定有重要影响,研究锡尔河-咸海三角洲湿地演变对认识流域生态环境变化、实施生态修复等具有重要意义。基于多源遥感数据采用面向对象分层提取法方法,分析1962—2019年锡尔河下游三角洲湿地的时空变...咸海周边环境变化对中亚干旱区生态稳定有重要影响,研究锡尔河-咸海三角洲湿地演变对认识流域生态环境变化、实施生态修复等具有重要意义。基于多源遥感数据采用面向对象分层提取法方法,分析1962—2019年锡尔河下游三角洲湿地的时空变化特征,并结合咸海变化、耕地变化、河流水量和水利工程建设等探讨近60a湿地变化的驱动因素。研究表明:①近60 a在咸海持续萎缩的情况下,锡尔河下游三角洲湿地呈现先减小后增加的变化趋势,其中1962—1980年、1981—1991年和1992—2019年3个阶段的变化率分别为-38.29 km 2/a、193.35 km 2/a和14.36 km 2/a;②湿地变化有明显的空间差异,锡尔河南、北2个入湖口区的湿地自1980年以来向咸海湖区扩张明显,耕地混合区的湿地自1991年以来向河道两侧扩张;③1962年后在入三角洲径流量总体减少的趋势下,灌渠建设和生态用水配置是三角洲湿地面积增加的主要原因,间接导致咸海入湖水量减少,咸海快速萎缩。锡尔河三角洲湿地恢复与咸海干涸形成了明显的生态反差,不利于咸海周边生态的整体恢复。展开更多
AtERF4 （ethylene response factor） is a negative regulator in jasmonic acid mediated signal transduction pathway and ethylene mediated signal transduction pathway of Arabidopsis. It could respond to abscisic acid （...AtERF4 （ethylene response factor） is a negative regulator in jasmonic acid mediated signal transduction pathway and ethylene mediated signal transduction pathway of Arabidopsis. It could respond to abscisic acid （ABA） and ethylene stimulus ATSYR1 gene encodes a syntaxin localizing at the plasma membrane in Arabidopsis, which can be induced by abiotic stress. To identify mutation lines for gene functional analysis, real-time PCR was employed to detect the expression level of AtERF4 and ATSYR1 in homozygous T-DNA insertion mutant line, respectively. Real-time PCR is a powerful tool which can be used to detect steady-state mRNA levels specifically, sensitively and reproducibly. Comparing to other forms of quantitative RT-PCR, the amount of amplified products can be detected by real-time PCR instantly and thus is a preferable alternative. In this study, RNA with T-DNA inserting into exon could be detected in AtERF4 knock-out mutation line. The results indicated that AtERF4 had been trucked in transcription level. On the other hand, T-DNA inserting into the promoter of gene ATSYR1 had no effect on reducing the expression level ofATSYR1 gene. Further molecular and phenotype studies now are ongoing to clarify the potential consequences of AtERF4 and ATSYR1 deficiency in Arabidopsis展开更多
文摘根据烟草Nt-syr1基因mRNA序列设计特异引物,建立了SYBR Green I实时荧光定量PCR反应体系,对烟株打顶后叶片Nt-syr1基因进行了mRNA转录水平上的定量分析,为从分子生物学水平上研究烤烟钾素营养调控机理提供新的技术手段。该方法简单实用,获得的荧光定量PCR扩增曲线基线平整,指数区扩增明显,斜率大;稳定性和重现性好,变异系数小;循环阈值Ct与PCR起始模板量的对数值之间存在良好的线性关系。对基因表达结果分析表明,烟株打顶后1 h,Nt-syr1基因在叶片中强烈表达,表达量约是同期不打顶处理的480倍,随后逐渐降低。与打顶处理相比,打顶后涂抹生长调节剂可以降低其表达量。
基金supported by the Department of Ecology in Kazakh Research Institute of Soil Science and Agrochemistry named after Uspanov,Almaty,Kazakhstan
文摘The effects of human activities on the soil cover transformation in the eastern part of Kazakhstan were investigated during the period of 1956-2008.The results of the research for different soil types in Priaralye indicated that there was 643.3×103 hm2 solonchaks,accounting for 38.5 % of the total area（1670.5×10^3 hm^2） in 2008.Vast areas are occupied with dried lakeshore soil（311.1× 10^3 hm^2）,sandy soils（147.6×10^3 hm^2） and grey-brown desert soils and solonetzes（146.7×10^3 hm^2）.In 2001 the area of solonchak was 755×103 hm2 and decreased to 643.3×10^3 hm^2 in 2008,which due to the shrinkage of the Aral Sea,the areas of marsh and lakeshore solonchaks decreased with the increase of dried bottom of the Aral Sea.The level of soil cover transformation in the modern delta of the Syr-Darya River can be seen from the comparison of the results obtained from the different years in the study area.The area of solonchaks increased by 10×10^3 hm^2 and the area of alluvial-meadow salinizied soils increased by 17.9×10^3 hm^2 during the period of 1956-1969.It means that many non-salinizied soils were transformed into salinizied ones.Striking changes occurred in the structure of soil cover as a result of aridization.So,the researches in1969 significantly determined the areas of hydromorphic soils subjected to desertification（it was not fixed on the map before 1956）.Later,these soils were transformed into takyr-like soils.The area of takyr-like soils increased almost by 3 times for 34 years（from 1956 to 1990）.The long-term soil researches on soil cover transformation in Priaralye have shown that the tendencies of negative processes（salinization and deflation） are being kept and lead to further soil and eco-environment degradation in the region.
基金supported by the National Natural Science Foundation of China (42230708)the Joint CAS (Chinese Academy of Sciences) & MPG (Max-Planck-Gesellschaft) Research Project (HZXM20225001MI)the Tianshan Talent Project of Xinjiang Uygur Autonomous Region, China (2022TSYCLJ0056)。
文摘The shrinkage of the Aral Sea,which is closely related to the Amu Darya River,strongly affects the sustainability of the local natural ecosystem,agricultural production,and human well-being.In this study,we used the Bayesian Estimator of Abrupt change,Seasonal change,and Trend(BEAST)model to detect the historical change points in the variation of the Aral Sea and the Amu Darya River and analyse the causes of the Aral Sea shrinkage during the 1950–2016 period.Further,we applied multifractal detrend cross-correlation analysis(MF-DCCA)and quantitative analysis to investigate the responses of the Aral Sea to the runoff in the Amu Darya River,which is the main source of recharge to the Aral Sea.Our results showed that two significant trend change points in the water volume change of the Aral Sea occurred,in 1961 and 1974.Before 1961,the water volume in the Aral Sea was stable,after which it began to shrink,with a shrinkage rate fluctuating around 15.21 km3/a.After 1974,the water volume of the Aral Sea decreased substantially at a rate of up to 48.97 km3/a,which was the highest value recorded in this study.In addition,although the response of the Aral Sea's water volume to its recharge runoff demonstrated a complex non-linear relationship,the replenishment of the Aral Sea by the runoff in the lower reaches of the Amu Darya River was identified as the dominant factor affecting the Aral Sea shrinkage.Based on the scenario analyses,we concluded that it is possible to slow down the retreat of the Aral Sea and restore its ecosystem by increasing the efficiency of agricultural water use,decreasing agricultural water use in the middle and lower reaches,reducing ineffective evaporation from reservoirs and wetlands,and increasing the water coming from the lower reaches of the Amu Darya River to the 1961–1973 level.These measures would maintain and stabilise the water area and water volume of the Aral Sea in a state of ecological restoration.Therefore,this study focuses on how human consumption of recharge runoff affects the Aral Sea and provides scientific perspective on its ecological conservation and sustainable development.
基金supported by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2022R1A4A3024451 and NRF2023R1A2C3002386)a grant from Korea University。
文摘Systemin, the first peptide hormone identified in plants, was initially isolated from tomato(Solanum lycopersicum) leaves. Systemin mediates local and systemic wound-induced defense responses in plants, conferring resistance to necrotrophic fungi and herbivorous insects. Systemin is recognized by the leucine-rich-repeat receptor-like kinase(LRRRLK) receptor SYSTEMIN RECEPTOR1(SYR1), but how the systemin recognition signal is transduced to intracellular signaling pathways to trigger defense responses is poorly understood. Here, we demonstrate that SERK family LRR-RLKs function as coreceptors for SYR1 to mediate systemin signal transduction in tomato. By using chemical genetic approaches coupled with engineered receptors, we revealed that the association of the cytoplasmic kinase domains of SYR1 with SERKs leads to their mutual trans-phosphorylation and the activation of SYR1, which in turn induces a wide range of defense responses. Systemin stimulates the association between SYR1 and all tomato SERKs(SlSERK1,SlSERK3A, and SlSERK3B). The resulting SYR1-SlSERK heteromeric complexes trigger the phosphorylation of TOMATO PROTEIN KINASE 1B(TPK1b), a receptor-like cytoplasmic kinase that positively regulates systemin responses. Additionally,upon association with SYR1, SlSERKs are cleaved by the Pseudomonas syringae effector HopB1, further supporting the finding that SlSERKs are activated by systemin-bound SYR1. Finally, genetic analysis using Slserk mutants showed that SlSERKs are essential for systemin-mediated defense responses. Collectively, these findings demonstrate that the systeminmediated association of SYR1 and SlSERKs activates defense responses against herbivorous insects.
文摘咸海周边环境变化对中亚干旱区生态稳定有重要影响,研究锡尔河-咸海三角洲湿地演变对认识流域生态环境变化、实施生态修复等具有重要意义。基于多源遥感数据采用面向对象分层提取法方法,分析1962—2019年锡尔河下游三角洲湿地的时空变化特征,并结合咸海变化、耕地变化、河流水量和水利工程建设等探讨近60a湿地变化的驱动因素。研究表明:①近60 a在咸海持续萎缩的情况下,锡尔河下游三角洲湿地呈现先减小后增加的变化趋势,其中1962—1980年、1981—1991年和1992—2019年3个阶段的变化率分别为-38.29 km 2/a、193.35 km 2/a和14.36 km 2/a;②湿地变化有明显的空间差异,锡尔河南、北2个入湖口区的湿地自1980年以来向咸海湖区扩张明显,耕地混合区的湿地自1991年以来向河道两侧扩张;③1962年后在入三角洲径流量总体减少的趋势下,灌渠建设和生态用水配置是三角洲湿地面积增加的主要原因,间接导致咸海入湖水量减少,咸海快速萎缩。锡尔河三角洲湿地恢复与咸海干涸形成了明显的生态反差,不利于咸海周边生态的整体恢复。
基金Supported by National High Technology Program (2008ZX08004-002, 2009ZX08009-032B)Key Research Plan of Heilongjiang Province (GA06B103)Education Department Plan of Heilongjiang Province(11521021, 1152024)
文摘AtERF4 （ethylene response factor） is a negative regulator in jasmonic acid mediated signal transduction pathway and ethylene mediated signal transduction pathway of Arabidopsis. It could respond to abscisic acid （ABA） and ethylene stimulus ATSYR1 gene encodes a syntaxin localizing at the plasma membrane in Arabidopsis, which can be induced by abiotic stress. To identify mutation lines for gene functional analysis, real-time PCR was employed to detect the expression level of AtERF4 and ATSYR1 in homozygous T-DNA insertion mutant line, respectively. Real-time PCR is a powerful tool which can be used to detect steady-state mRNA levels specifically, sensitively and reproducibly. Comparing to other forms of quantitative RT-PCR, the amount of amplified products can be detected by real-time PCR instantly and thus is a preferable alternative. In this study, RNA with T-DNA inserting into exon could be detected in AtERF4 knock-out mutation line. The results indicated that AtERF4 had been trucked in transcription level. On the other hand, T-DNA inserting into the promoter of gene ATSYR1 had no effect on reducing the expression level ofATSYR1 gene. Further molecular and phenotype studies now are ongoing to clarify the potential consequences of AtERF4 and ATSYR1 deficiency in Arabidopsis
