Size-fractionated Chlorophyll α biomass in the northern South China Sea in summer 2014

Spatial distribution of phaeopigment and size-fractionated chlorophyll a(Chl a) concentrations were examined in relation to hydrographic conditions in the northern South China Sea(NSCS) during a survey from 20 August to 12 September, 2014. The total Chl a concentration varied from 0.006 to 1.488 μg/L with a mean value of 0.259±0.247(mean±standard deviation) μg/L. Chl a concentration was generally higher in shallow water(<200 m) than in deep water(>200 m), with mean values of 0.364±0.311 μg/L and 0.206±0.192 μg/L respectively. Vertically, the maximum total Chl a concentration appeared at depths of 30–50 m and gradually decreased below 100 m. The size-fractionated Chl a concentrations of grid stations and time-series stations(SEATS and J4) were determined, with values of pico-(0.7–2 μm), nano-(2–20 μm) and micro- plankton(20–200 μm) ranging from 0.001–0.287(0.093±0.071 μg/L), 0.004–1.149(0.148±0.192 μg/L) and 0.001–0.208(0.023±0.036 μg/L), respectively. Phaeopigment concentrations were determined at specifi c depths at ten stations, except for at station A9, and varied from 0.007 to 0.572(0.127±0.164) μg/L. Nano-and pico-plankton were the major contributors to total phytoplankton biomass, accounting for 50.99%±15.01% and 39.30%±15.41%, respectively, whereas microplankton only accounted for 9.39%±8.66%. The results indicate that the contributions of microplankton to total Chl a biomass were less important than picoplankton or nanoplankton in the surveyed NSCS. Diff erent sized-Chl a had similar spatial patterns, with peak values all observed in subsurface waters(30–50 m). The summer monsoon, Kuroshio waters, Zhujiang(Pearl) River plume, and hydrological conditions are speculated to be the factors controlling the abundance and spatial heterogeneity of Chl a biomass in the NSCS.

Variability of size-fractionated chlorophyll a in the high-latitude Arctic Ocean in summer 2020

The size structure of phytoplankton has considerable effects on the energy flow and nutrient cycling in themarine ecosystem,and thus is important to marine food web and biological pump.However,its dynamics in the high-latitude Arctic Ocean,particularly ice-covered areas,remain poorly understood.We investigated size-fractionated chlorophyll a(Chl a)and related environmental parameters in the highly ice-covered Arctic Ocean during the summer of 2020,and analyzed the relationship between Chl adistribution and water mass through cluster analysis.Results showed that inorganic nutrients were typically depleted in the upper layer of the Canada Basin region,and that phytoplankton biomass was extremely low(mean=0.05±0.18 mg·m^(−3))in the near-surface layer(upper 25 m).More than 80%of Chl a values were<0.1 mg·m^(−3) in the water column(0-200 m),but high values appeared at the ice edge or in corresponding ice areas on the shelf.Additionally,the mean contribution of both nanoplankton(2-20μm)(41%)and picoplankton(<2μm)(40%)was significantly higher than that of microplankton(20-200μm)(19%).Notably,the typical subsurface chlorophyll maximum(0.1 mg·m^(−3))was found north of 80°N,where the concentration of sea ice reached approximately 100%.The Chl aprofile results showed that the deep chlorophyll maximum of total-,micro-,nano-,and picoplankton was located at depth of 40,39,41,and 38 m,respectively,indicating that nutrients are the primary factor limiting phytoplankton growth in the ice-covered Arctic Ocean during summer.These phenomena suggest that,despite the previous literatures pointing to significant light limitation under the Arctic ice,the primary limiting factor for phytoplankton in summer is still nutrient.

Distribution characteristics of size-fractionated chlorophyll a, primary production and new production in the Laizhou Bay, July 1997

The distributions of chlorophyll a concentration, primary production and new productionwere observed in the Laizhou Bay of the Bohai Sea in both spring and neap tides during July 1997. The results showed that there were marked features of spatial zonation in the surveyed area, due to the differences between the geographic environment and the hydrological conditions. Chlorophyll a, primary production and new production were all higher in spring tides than that in neap tides in the Laizhou Bay. The highest values of these parameters were encountered in the central regions of the bay. At most stations, chlorophyll a concentrations at the bottom were higher than that at the surface. The results of size-fractionated chlorophyll a and primary production showed that contributions of nanocombining pi-coplankton ( <20 μm) to total chlorophyll a and primary production were dominant in phytoplankton community biomass and production of the Laizhou Bay. The environmental factors, primary production and new production in the Laizhou Bay are compared with other sea areas.

The size-fractionated chlorophyll a and primary productivity in the Bering Sea during the summer of 2003

Investigations of chlorophyll a and primary productivity were carried out inthe Bering Sea along the BR line and the BS line during the Second Chinese National Arctic ResearchExpedition in the summer of 2003.The results showed that the surface chlorophyll a concentrationswere 0.199~1.170μg/dm^(3),and the average value was 0.723μg/dm^(3) on the BR line.For the BSline,the surface chlorophyll a concentrations were 0.519~4.644μg/dm^(3)(average 1.605μg/dm^(3))and 0.568~14.968μg/dm^(3)(average 5.311μg/dm^(3))during the early and late summer,respectively.The average value in the late summer was much higher than that in the early summer.The high values(more than 4.0μg/dm^(3))occurred at stations of the BS line in the southern Bering Strait.Thechlorophyll a concentrations in the subsurface layer were higher than those in the surface layer.The results of the size-fractionated chlorophyll a showed that the contribution of the picoplanktonto total chlorophyll a was the predominance at the early summer and the contribution of thenetplankton was the predominance at the late summer.The carbon potential primary productivitiesvaried between 0.471 and 1.147 mg/(m^(3)·h)on the BR line,with average rates of 0.728 mg/(m^(3)·h).The primary productivities on the BS line were much higher than those of the BR line,ranging from1.227 mg/(m^(3)·h)at the early summer to 19.046 mg/(m^(3)·h)at the late summer.The results of thesize-fractionated primary productivity showed that the contribution of the nanoplankton to totalproductivity was the predominance at the early summer and the contribution of the netplankton waspredominance at the late summer.The assimilation number of photosynthesis was 0.45~2.80 mg/(mg·h)in the surveyed stations.

The distribution feature of size-fractionated chlorophyll a and primary productivity in Prydz Bay and its north sea area during the austral summer

The investigation of size-fractionated chlorophyll a and primary productivity were carried out in three longitudinal sections (63°-69°12'S, 70°30'E, 73°E and 75(30'E) at December 18-26, 1998 and January 12-18, 1999 in Prydz Bay and its north sea area, Antarctica. The results showed that surface chlorophyll a concentration were 0. 16 -3. 99 μg dm-3. The high values of chlorophyll a concentration (more than 3.5 μg dm -3) were in Prydz Bay and in the west Ladies Bank. The average chlorophyll a concentration at sub-surface layer was higher than that at surface layer; its concentration at the deeper layers of 50 m decreased with increasing depth and that at 200 m depth was only 0. 01 -0. 95μg dm -3. The results of size-fractionated chlorophyll a showed that the contribution of the netplanktion to total chlorophyll a was 56% , those of the nanoplankton and the picoplankton were 24% and 20% respectively in the surveyed area. The potential primary productivity at the euphotic zone in the surveyed area was 0. 11 - 11. 67 mgC m-3h-1 and average value was 2.00 ±2.80 mgC m h . The in-situ productivity in the bay and the continental shelf was higher and that in the deep-sea area was lower. The assimilation number of photosynthesis was 1.53±1. 11 mgC/(mg Chi a · h). The results of size-fractionated primary productivity show that the contribution of the netplanktion to total productivity was 58% , those of the nanoplankton and the picoplankton were 26% and 16% respectively. The cell abundance of phytoplankton was 1. 6 × 103 - 164. 8 × 103 cell dm-3 in the surface water.

Distribution characteristics of size-fractionated chlorophyll a and primary productivity in Beibu Gu

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Ethylene accelerates maize leaf senescence in response to nitrogen deficiency by regulating chlorophyll metabolism and autophagy

Leaf senescence is an orderly and highly coordinated process,and finely regulated by ethylene and nitrogen(N),ultimately affecting grain yield and nitrogen-use efficiency(NUE).However,the underlying regulatory mechanisms on the crosstalk between ethylene-and N-regulated leaf senescence remain a mystery in maize.In this study,ethylene biosynthesis gene ZmACS7 overexpressing(OE-ZmACS7)plants were used to study the role of ethylene regulating leaf senescence in response to N deficiency,and they exhibited the premature leaf senescence accompanied by increased ethylene release,decreased chlorophyll content and F_v/F_m ratio,and accelerated chloroplast degradation.Then,we investigated the dynamics changes of transcriptome reprogramming underlying ethylene-accelerated leaf senescence in response to N deficiency.The differentially expressed genes(DEGs)involved in chlorophyll biosynthesis were significantly down-regulated,while DEGs involved in chlorophyll degradation and autophagy processes were significantly up-regulated,especially in OE-ZmACS7 plants in response to N deficiency.A gene regulatory network(GRN)was predicted during ethylene-accelerated leaf senescence in response to N deficiency.Three transcription factors(TFs)ZmHSF4,Zmb HLH106,and ZmEREB147 were identified as the key regulatory genes,which targeted chlorophyll biosynthesis gene ZmLES22,chlorophyll degradation gene ZmNYC1,and autophagy-related gene ZmATG5,respectively.Furthermore,ethylene signaling key genes might be located upstream of these TFs,generating the signaling cascade networks during ethylene-accelerated leaf senescence in response to N deficiency.Collectively,these findings improve our molecular knowledge of ethylene-accelerated maize leaf senescence in response to N deficiency,which is promising to improve NUE by manipulating the progress of leaf senescence in maize.

Spatial correlations in time and frequency domains between chlorophyll-a concentration and environmental factors in the Bohai Sea

Based on the reconstructed MODIS data and ECMWF reanalysis data from 2003 to 2021,spatial correlations between chlorophyll a(Chl a)and sea surface temperature(SST),photosynthetically available radiation(PAR),aerosol optical thickness(AOT),and wind speed(WS)in the Bohai Sea were analyzed from the perspective of time domain and frequency domain.Results indicate that the frequency domain analysis was more conducive to revealing the correlations between Chl a and environmental factors.The spatial pattern of time-domain correlations was similar to the isobaths of the Bohai Sea,which was positive in shallow waters and negative in deep waters for SST,PAR,and AOT,and was reversed for WS.Frequency-domain correlations were obtained by performing Fourier Transform and were higher than correlations in time domain.The spatial distributions indicated that the effects of SST and PAR on Chl a were greater than AOT and WS in the Bohai Sea.Additionally,cross-spectrum analysis was applied to explore the response relationships.A depth-dependent pattern was shown in correlations and time lags,indicating that the influential mechanism of environmental factors on Chl-a concentration is related to seawater depth.

Degree of shade tolerance shapes seasonality of chlorophyll, nitrogen and phosphorus levels of trees and herbs in a temperate deciduous forest

Forest productivity is closely linked to seasonal variations and vertical differentiation in leaf traits.However,leaf structural and chemical traits variation among co-existing species,and plant functional types within the canopy are poorly quantified.In this study,the seasonality of leaf chlorophyll,nitrogen(N),and phosphorus(P)were quantified vertically along the canopy of four major tree species and two types of herbs in a temperate deciduous forest.The role of shade tolerance in shaping the seasonal variation and vertical differentiation was examined.During the entire season,chlorophyll content showed a distinct asymmetric unimodal pattern for all species,with greater chlorophyll levels in autumn than in spring,and the timing of peak chlorophyll per leaf area gradually decreased as shade tolerance increased.Chlorophyll a:b ratios gradually decreased with increasing shade tolerance.Leaf N and P contents sharply declined during leaf expansion,remained steady in the mature stage and decreased again during leaf senescence.Over the seasons,the lower canopy layer had significantly higher chlorophyll per leaf mass but not chlorophyll per leaf area than the upper canopy layer regardless of degree of shade tolerance.However,N and P per leaf area of intermediate shade-tolerant and fully shade-tolerant tree species were significantly higher in the upper canopy than in the lower.Seasonal variations in N:P ratios suggest changes in N or P limitation.These findings indicate that shade tolerance is a key feature shaping inter-specific differences in leaf chlorophyll,N,and P contents as well as their seasonality in temperate deciduous forests,which have significant implications for modeling leaf photosynthesis and ecosystem production.

Spatio-temporal variability of surface chlorophyll a in the Yellow Sea and the East China Sea based on reconstructions of satellite data of 2001-2020

Chlorophyll-a(Chl-a)concentration is a primary indicator for marine environmental monitoring.The spatio-temporal variations of sea surface Chl-a concentration in the Yellow Sea(YS)and the East China Sea(ECS)in 2001-2020 were investigated by reconstructing the MODIS Level 3 products with the data interpolation empirical orthogonal function(DINEOF)method.The reconstructed results by interpolating the combined MODIS daily+8-day datasets were found better than those merely by interpolating daily or 8-day data.Chl-a concentration in the YS and the ECS reached its maximum in spring,with blooms occurring,decreased in summer and autumn,and increased in late autumn and early winter.By performing empirical orthogonal function(EOF)decomposition of the reconstructed data fields and correlation analysis with several potential environmental factors,we found that the sea surface temperature(SST)plays a significant role in the seasonal variation of Chl a,especially during spring and summer.The increase of SST in spring and the upper-layer nutrients mixed up during the last winter might favor the occurrence of spring blooms.The high sea surface temperature(SST)throughout the summer would strengthen the vertical stratification and prevent nutrients supply from deep water,resulting in low surface Chl-a concentrations.The sea surface Chl-a concentration in the YS was found decreased significantly from 2012 to 2020,which was possibly related to the Pacific Decadal Oscillation(PDO).

Antioxidase System of a Rice Mutant with Low Chlorophyll b

[Objective] The mitigative effect of antioxidase system of a rice mutant with low chlorophyll b on photooxidative damage was studied.[Method] A rice mutant with low chlorophyll b and its wild type were taken as experimental materials to comparatively research their peroxide (H2O2) contents, the activity and isozymes of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in chloroplast.[Result] Compared with the wild type, there were many kinds of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant, and the activity of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant was also correspondingly higher. Under intense light condition, the H2O2 content of chloroplast in mutant was less than that in the wild type. [Conclusion] The higher activity of scavenging active oxygen can relieve the photooxidative damage made by excessive light energy of intense light on photosynthetic membrane, which is an important reason for higher photosystem Ⅱ (PS II) stability of this mutant.

Study on a Mutant with Low Content Chlorophyll b in a High Yielding Rice and Its Photosynthesis Properties

A high yielding rice mutant ( Oryza sativa L. cv. Zhenhui 249) with low chlorophyll b was recently discovered in the field. The mutant was mainly characterized by the decrease of the content of extrinsic antennae complex. This variation was shown in the stage when the leaves were expanding. When the leaves are at the final developmental stage, the content would approach to that of the wild type. It was discovered that only moderate amount of chlorophyll b decreased in this mutant. The photosynthetic apparatus of the mutant was rather stable in the whole life span of the leaf. The extrinsic antennae complex of the mutant might make efficient use of light and meanwhile reduce the production of O -· 2.

The Chlorophyll Biosynthesis in Lotus Embryo Is Light-dependent

Angiosperms need light to synthesize chlorophyll, but lotus (Nelumbo nucifera Gaertn.) embryo was suspected to have the ability to form chlorophyll in the dark because lotus embryo can turn into green under the coverage of four layers of integuments (cotyledon, seed coat, pericarp, lotus pod) which were thought impossible for light to pass through. The authors excluded this possibility based on two experimental results: First, enclosing the young lotus pod with aluminium foil, the growth of louts embryo continued, but the chlorophyll formation was seriously inhibited. A lot of protochlorophyllide, chlorophyll precursor, were accumulated, most of which were combined with LPOR (light dependent protochlorophyllide oxidoreductase). Second, DPOR (dark or light-independent protochlorophyllide oxidoreductase) was the enzyme necessary for chlorophyll synthesis in the dark. The genes encoding DPOR were conservative in many species, but no homologues could be found in lotus genome. Taken together, authers' results clearly demonstrated that lotus embryo synthesizes chlorophyll only through the light-dependent pathway.

Correlation Analysis of SPAD Value with Chlorophyll Content and Economic Yield Traits of Brassica napus L.

[Objective] The aim was to compare differences of SPAD value, chloro- phyll content, agronomic characters, economic characters and yield traits to analyze correlation of SPAD value with other indices and establish regression functions. [Method] Based on 34 Brassica napus L. varieties, SPAD value, chlorophyll content, agronomic characters, economic characters and yield traits were measured and re- gression functions were established according to correlations. [Result] SPAD value, chlorophyll content, agronomic and economic characters and yield traits all achieved significant level in differences among 34 varieties. Specifically, SPAD value was of extremely significant correlation with chlorophyll a and b, total chlorophyll and carotenoid, and the correlation from high to low was chl-b〉chl-z〉chl-a〉chl-x. SPAD value was of significantly positive correlation with total pod number per plant, plant height, seed number per pod, yield per plant and harvest yield, and of insignificant correlation with branch point height, effective branch number, pod density of main stem, and pod length. [Conclusion] It is simple and rapid to predict chlorophyll con- tent, economic characters and yields of Brassica napus L. with SPAD value and re- gression functions.

Grey Relational Analysis between Chlorophyll a and Environmental Factors in ShaHu Lake

[Objective] The aim was to explore effects of environmental factors on the content of Chlorophyll a in ShaHu Lake.[Method] Based on the data in Shahu Lake from November in 2007 to September in 2008,the relationship between chlorophyll a and environmental factors like water temperature,pH,secchi-depth （SD）,total nitrogen,total phosphorus and potassium permanganate index was studied by grey relational analysis method.[Result] The main environmental factors affecting the content of Chlorophyll a in ShaHu Lake were in order of water temperature potassium permanganate index 〉total nitrogen 〉pH〉 total phosphorus 〉SD.[Conclusion] The research provides reference for the control of eutrophication and the reasonable development and utilization of Shahu Lake.

Characteristics of Chlorophyll Fluorescence and Membrane-lipid Peroxidation of Various High-yield Rices Under Photooxidation Conditions

Using various high-yield rices (Oryza sativa L.) such as japonica cultivar 9516, two parental line hybrid rice between subspecies with more japonica element Peiai 64/E32, Liangyoupeijiu (Peiai 64/9311), and indica hybrid rices X07S/Zihui 100, Gangyou 881, Shanyou 63 as the materials, the characteristics of chlorophyll fluorescence and membrane-lipid peroxidation of detached leaves at booting stage under photooxidation conditions were studied. In comparison with indica hybrid rice, after the photooxidation treatment, the primary photochemical efficiency of PS II (F-v/F-m), quantum yield of linear electron transport of PS II (Phi(PSII)) and photochemical quenching coefficient (qP) in japonica cultivar and hybrid rice with japonica decreased less. This indicated that high-yield rice with japonica was able to maintain higher capability of light energy conversion, resulting in the alleviation of photoinhibition. Meanwhile, the higher activities of protective enzymes such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) led to the less accumulation of endogenous active oxygen (O-(2)(radical anion), H2O2) and less content of the malondialdehyde (MDA) and the less decline of chlorophyll and protein contents, indicating a stronger tolerance to photooxidation. The changes in contents of chlorophyll and protein among various nee cultivars during photooxidation treatment were consistent with the decline of chlorophyll content from heading stage to maturation stage under natural conditions. Statistical analysis showed that there was a significant correlation between the indexes of tolerance to photooxidation and the rate of seed setting, implying that the cultivar tolerated to photooxidation had higher resistance to early aging of leaf. These results suggested that from a view of superhigh-yield breeding, considering both the utilization of heterosis and the resistance to early aging of leaf, introduction of japonica element tolerating to photooxidation into the rice sterile line (maternal plant) is a breeding strategy worthy to pay great attention to.

Characteristics of CO_2 Exchange and Chlorophyll Fluorescence of Transgenic Rice with C_4 Genes

The responses of photosynthesis of phosphoenopyruvate carboxylase (PEPC), pyrurate dikinase (PPDK), NADP-malic enzyme (NADP-ME) and PPDK+PEPC transgenic rice (Oryza saltiva L.) plant to light, temperature, CO 2 and the characteristics of chlorophyll fluorescence under photoinhibition conditions were studied. The results were as follows: 1. The light-saturated photosynthetic rates of transgenic rice plants were higher than that of wild type, in which the light-saturated point of PEPC and PPDK+PEPC transgenic rice plants was 200 μmol·m -2·s -1 higher than that of untransformed rice and the light-saturated photosynthetic rates were 51.6% and 58.5% respectively. The carboxylation efficiency of PEPC transgenic rice plant increased by 49.3% and the CO 2 compensation point decreased by 26.2% than that of untransformed rice. Under high temperature (35 ℃), the photosynthetic rate of PEPC transgenic rice plant was higher over 17.5% than that of untransformed rice. 2. On the 8th day after photoinhibition treatment, the PSⅡ photochemical efficiency (F v/F m) and photochemical quenching (qP) of PEPC and PPDK+PEPC transgenic rice plants decreased by about 20%-30% while the non-photochemical quenching (qN) increased by approximately 30%. But F v/F m and qP of untransformed rice decreased by over 50% while qN increased by less than 10%. The result suggested that transgenic rice plants were more tolerant to photoinhibition.

A REMOTE SENSING MODEL FOR DETERMINING CHLOROPHYLL CONTENT AND ITS DISTRIBUTION USING LANDSAT IMAGES

The uncertainty in the estimatation of chlorophyll content with the use of normalized difference vegetation index (NDVI) has been described. To determine the chlorophyll content, model 1 for LANDSAT and model 2 for NOAA AVHRR wavebands were presented and have been verified by field experiments. Model 1 was also validated by the distribution of chlorophyll content using LANDSAT images around the Yucheng remote sensing experimental station. Using these models to estimate the chlorophyll content in the vegetation community is benefitiated by the increased precision and decreased uncertainty.

Three-stage Transformation of Chlorophyll Transient Fluorescence Pattern Under Sustained Dehydration and the Discovery of Critical Water Content in Seaweeds

The chlorophyll fluorescence kinetics of marine red alga Grateloupia turutunt Yamada, green alga Ulva pertusa Kjellm and brown alga Laminaria japonica Aresch during natural sustained dehydration were monitored and investigated. The pulse amplified modulation (PAM) system was used to analyze the distinct fluorescence parameters during thallus dehydration. Results proved that the fluorescence kinetics of different seaweed all showed three patterns of transformation with sustained water loss. These were: 1) peak kinetic pattern (at the early stage of dehydration fluorescence enhanced and quenched subsequently, representing a normal physiological state). 2) plateau kinetic pattern (with sustained water loss fluorescence enhanced continuously but quenching became slower, finally reaching its maximum). 3) Platform kinetic pattern (fluorescence fell and the shape of kinetic curve was similar to plateau kinetic pattern). A critical water content (CWC) could be found and defined as the percentage of water content just prior to the fluorescence drop and to be a significant physiological index for evaluation of plant drought tolerance. Once thallus water content became lower than this value the normal peak pattern can not be recovered even through rehydration, indicating an irreversible damage to the thylakoid membrane. The CWC value corresponding to different marine species were varied and negatively correlated with their desiccation tolerance, for example. Laminaria japonica had the highest CWC value (around 90%) and the lowest dehydration tolerance of the three. In addition, a fluorescence 'burst' was found only in red algae during rehydration. The different fluorescence parameters F-o, F-v and F-v, F-m were measured and compared during water loss. Both F-o and F-v increased in the first stage of dehydration but F-v/F-m. kept almost constant. So the immediate response of in vivo chlorophyll fluorescence to dehydration was an enhancement. Later with sustained dehydration F-o increased continuously while F-v decreased and tended to become smaller and smaller. The major changes in fluorescence (including fluorescence drop during dehydration and the burst during rehydration) were all attributed to the change in F-o instead of F-v This significance of F-o indicates that it is necessary to do more research on F-o as well as on its relationship with the state of thylakoid membrane.

Effects of Salicylic Acid on Photosynthesis and Chlorophyll Fluorescence Characteristics of Fluecured Tobacco Leaf in Subdued Light

[Objective] This study aimed to test whether salicylic acid （SA） can im-prove the physiological functions of flue-cured tobacco under subdued light condition, and to determine the mechanism of its action. [Method] The tobacco plants under subdued light were foliar-sprayed with 100 mg/L of SA. Then, the physiological in-dices such as plant fresh weight and dry weight, chlorophyl content, photosynthetic parameters and chlorophyl fluorescence parameters were measured. SPSS17.0 and Excellwere adopted for variance analysis and significance test. [Result] The leaf photosynthetic rate （Pn）, stomatal conductance （Gs） and transpiration rate （Tr） of tobacco plants in subdued light were al decreased while the intercellular CO2 con-centration （Ci） was increased, suggesting that non-stomatal limitation led to the de-crease of Pn under weak light intensity stress. SA released the inhibition of tobacco plant growth in weak light, as it elevated the leaf photosynthetic rate, the maximum photochemical efficiency of PSⅡ, potential activity of PSⅡ, effective photochemical efficiency of PSⅡ and photochemical quenching coefficient in weak light significant-ly, and reduced the non-photochemical quenching coefficient. [Conclusion] SA has significant effects on the photosynthetic characteristics of flue-cured tobacco in weak light, and it can improve the synthesis or distribution of photosynthesis product, and the efficiency of light energy, conducive to plant growth and development.