Regulation of Hypoxic Response Elements on the Expression of Vascular Endothelial Growth Factor Gene Transfected to Rat Skeletal Myoblasts under Hypoxic Environment

The regulation of hypoxic response elements on the expression of vascular endothelial growth factor （VEGF） gene transfected to primary cultured rat skeletal myoblasts under hypoxic environment was investigated. pEGFP-C3-9HRE-CMV-VEGF vector was constructed with molecular biology technique and transfected to primary cultured rat skeletal myoblasts by lipofectamine in vitro. Gene expression of transfected myoblasts was detected by RT-PCR, Western blot and fluorescence microscope under different oxygen concentrations and different hypoxia time. The results showed that in hypoxia group, the VEGF gene bands were seen and with the decrease of oxygen concentrations and prolongation of hypoxia time, the expression of VEGF mRNA was obviously increased. Under hypoxic environment, the expression of VEGF protein in the transfected myoblasts was significantly increased. EGFP was expressed only under hypoxic environment but not under normoxic environment. It was concluded that hypoxia promoter could be constructed with HRE and regulate the expression of VEGF gene under hypoxic and normoxic environment, which could enhance the re- liability of gene therapy.

失神经骨骼肌再生过程中成肌干细胞的调控

肌卫星细胞是成体骨骼肌中最基本的成肌干细胞，在骨骼肌生长、再生过程中起决定性作用。当外周神经损伤后，静息态的肌卫星细胞激活并增殖，大部分分化为成肌细胞后相互融合形成肌管或与受损肌纤维直接融合，参与骨骼肌的再生；另一部分则恢复静息状态，通过自我更新过程维持成肌干细胞库的稳定。本文将对失神经骨骼肌损伤及再生过程中成肌干细胞的功能改变及相关调控机制的研究文献进行综述。