The skin is a formidable physical and biological barrier which communicates continuously with the outside of the body. And the stratum corneum, the outermost layer of human epidermis, plays a central role in the inter...The skin is a formidable physical and biological barrier which communicates continuously with the outside of the body. And the stratum corneum, the outermost layer of human epidermis, plays a central role in the interaction between the cutaneous tissue and the external environment. The horny layer, and more generally the whole skin layers, avoid the penetration of harmful exogenous agents, produce molecules named anti-microbial peptides which impact the composition of the cutaneous microbiota, regulate the internal corporal temperature, avoid the water loss from the inside of the body and constitute an incredible efficient anti-oxidant network. Nevertheless, nowadays, the skin is more and more solicited by the different elements of the cutaneous exposome, including atmospheric pollution and solar radiations, which can cause a dramatic acceleration of the skin ageing process. As a consequence, due to the multifunctional protective role of the skin, during the recent decade the cosmetic industry invested massively in the development of new raw materials and end-products (dermo-cosmetics) able to preserve an optimal state of the skin regarding the external environment. Based on their physical-chemical properties thermal spring waters, which are extremely rich in inorganics ions, are interesting and powerful candidates to be part, as integral component, of new efficient dermo-cosmetic formulations dedicated to protect the skin from the external stimuli. The aim of the present work was to investigate and characterize the activity of Jonzac thermal spring water on the skin. Using different models, we proved for the first time that Jonzac thermal spring water reinforces the barrier function of the skin by modulating the expression of key markers including filaggrin and human beta defensin 2 on ex vivo human skin. The ex vivo and in vivo hydration activity, by Raman spectroscopy and corneometry respectively, has been also demonstrated. We have also shown that Jonzac thermal spring water ameliorates significantly the cutaneous microrelief in vivo. To conclude, we characterize the soothing effect of Jonzac thermal spring water by the analysis of histamine release in Substance P treated skin explants and by measuring the redness of the skin following UV exposure of the skin in vivo. We observed that both parameters decreased following a preventive treatment of the skin with Jonzac thermal spring water. Taken together our results indicate that Jonzac thermal spring water is a promising and powerful dermo-cosmetic which can be used to preserve an optimal state of the cutaneous tissue.展开更多
The physiological skin surface pH is just below 5. With age the skin surface pH increases up to 6. An increased pH correlates with reduced barrier integrity/cohesion. The present pilot study assesses possible normaliz...The physiological skin surface pH is just below 5. With age the skin surface pH increases up to 6. An increased pH correlates with reduced barrier integrity/cohesion. The present pilot study assesses possible normalization of an increased skin surface pH of the elderly and improvement of barrier function via application of ≈pH 4.0 skin care products. Baseline skin surface pH was determined in elderly (80+ years old;n = 15) compared to middle aged adults (31 - 50 years old;n = 15). The effect of o/w emulsions at pH-values of 3.5, 4.0, 4.5 and 5.5 on the skin surface pH was determined in both groups. Further, the effect of a 4-week treatment with a pH 4.0 skin care product on the skin surface pH, skin hydration and barrier integrity was assessed. Thirteen elderly females were involved in this home-in-use test. Increased baseline skin surface pH of the elderly normalizes to the physiological pH of 4.5 - 5.0 over 7 hours after single application of o/w-emulsions with a given pH of 3.5 or 4.0. A 4 week treatment employing the pH 4.0 skin care product improves the epidermal barrier integrity of the elderly significantly (p = 0.005). Reduction of the increased baseline skin surface pH of the elderly is accompanied by improved epidermal barrier integrity. Skin care products for the elderly have to be adjusted in the pH range of 3.5 to 4.0.展开更多
目的研究中药挥发油透皮促渗苷类成分效果与对经皮水分散失量(Transepidermal water loss,TEWL)的相关性。方法采用HPLC法测定3种苷类成分(栀子苷、龙胆苦苷、芍药苷)的含量及油水分配系数,采用改良Franz扩散池法以离体SD大鼠腹部皮肤...目的研究中药挥发油透皮促渗苷类成分效果与对经皮水分散失量(Transepidermal water loss,TEWL)的相关性。方法采用HPLC法测定3种苷类成分(栀子苷、龙胆苦苷、芍药苷)的含量及油水分配系数,采用改良Franz扩散池法以离体SD大鼠腹部皮肤为透皮吸收屏障进行体外透皮实验,考察高良姜、干姜、薄荷、胡椒和吴茱萸5种中药挥发油对3种苷类成分的体外透皮促渗效果,采用经皮水分散失量评价5种中药挥发油对大鼠皮肤屏障功能的影响,通过相关性分析考察中药挥发油透皮促渗苷类成分效果与其对皮肤屏障功能影响之间的相关性。结果高良姜油、干姜油、薄荷油、胡椒油能促进3种苷类成分的透皮吸收,也能显著降低大鼠的皮肤屏障功能,相关性分析结果表明中药挥发油对苷类成分的透皮促渗效果与其对皮肤经皮水分散失量的影响显著相关。结论TEWL为中药挥发油透皮促渗剂的快速筛选提供了一种简便快捷的在体评价方法。展开更多
目的:探讨含龙胆草提取物的液体敷料联合依巴斯汀治疗面部敏感性皮肤的疗效及其对皮肤生理功能的影响。方法:按照随机数字表法将2021年10月-2022年12月笔者医院收治的90例面部敏感性皮肤患者分为对照组与观察组,每组45例。对照组使用依...目的:探讨含龙胆草提取物的液体敷料联合依巴斯汀治疗面部敏感性皮肤的疗效及其对皮肤生理功能的影响。方法:按照随机数字表法将2021年10月-2022年12月笔者医院收治的90例面部敏感性皮肤患者分为对照组与观察组,每组45例。对照组使用依巴斯汀治疗,观察组使用含龙胆草提取物的液体敷料联合依巴斯汀治疗,均连续治疗4周。比较两组的疗效,乳酸刺激试验指标,皮肤生理功能指标[经皮水分丢失量(Transepidermal water loss,TEWL)、皮脂含量、角质层含水量、皮肤pH值]及不良反应。结果:治疗结束后,观察组的总有效率(93.33%vs 71.11%)明显高于对照组(P<0.05);两组乳酸刺激试验指标,皮肤生理功能指标均治疗前明显改善(P<0.05);观察组乳酸刺痛评分明显低于对照组(P<0.05),乳酸刺痛感开始时间明显长于对照组(P<0.05);观察组TEWL、皮肤pH值明显低于对照组(P<0.05),皮脂含量、角质层含水量明显高于对照组(P<0.05)。两组均无严重不良反应发生。结论:含龙胆草提取物的液体敷料联合依巴斯汀能明显提高面部敏感性皮肤的临床疗效,明显改善皮肤生理功能及耐受性,安全性好。展开更多
文摘The skin is a formidable physical and biological barrier which communicates continuously with the outside of the body. And the stratum corneum, the outermost layer of human epidermis, plays a central role in the interaction between the cutaneous tissue and the external environment. The horny layer, and more generally the whole skin layers, avoid the penetration of harmful exogenous agents, produce molecules named anti-microbial peptides which impact the composition of the cutaneous microbiota, regulate the internal corporal temperature, avoid the water loss from the inside of the body and constitute an incredible efficient anti-oxidant network. Nevertheless, nowadays, the skin is more and more solicited by the different elements of the cutaneous exposome, including atmospheric pollution and solar radiations, which can cause a dramatic acceleration of the skin ageing process. As a consequence, due to the multifunctional protective role of the skin, during the recent decade the cosmetic industry invested massively in the development of new raw materials and end-products (dermo-cosmetics) able to preserve an optimal state of the skin regarding the external environment. Based on their physical-chemical properties thermal spring waters, which are extremely rich in inorganics ions, are interesting and powerful candidates to be part, as integral component, of new efficient dermo-cosmetic formulations dedicated to protect the skin from the external stimuli. The aim of the present work was to investigate and characterize the activity of Jonzac thermal spring water on the skin. Using different models, we proved for the first time that Jonzac thermal spring water reinforces the barrier function of the skin by modulating the expression of key markers including filaggrin and human beta defensin 2 on ex vivo human skin. The ex vivo and in vivo hydration activity, by Raman spectroscopy and corneometry respectively, has been also demonstrated. We have also shown that Jonzac thermal spring water ameliorates significantly the cutaneous microrelief in vivo. To conclude, we characterize the soothing effect of Jonzac thermal spring water by the analysis of histamine release in Substance P treated skin explants and by measuring the redness of the skin following UV exposure of the skin in vivo. We observed that both parameters decreased following a preventive treatment of the skin with Jonzac thermal spring water. Taken together our results indicate that Jonzac thermal spring water is a promising and powerful dermo-cosmetic which can be used to preserve an optimal state of the cutaneous tissue.
文摘The physiological skin surface pH is just below 5. With age the skin surface pH increases up to 6. An increased pH correlates with reduced barrier integrity/cohesion. The present pilot study assesses possible normalization of an increased skin surface pH of the elderly and improvement of barrier function via application of ≈pH 4.0 skin care products. Baseline skin surface pH was determined in elderly (80+ years old;n = 15) compared to middle aged adults (31 - 50 years old;n = 15). The effect of o/w emulsions at pH-values of 3.5, 4.0, 4.5 and 5.5 on the skin surface pH was determined in both groups. Further, the effect of a 4-week treatment with a pH 4.0 skin care product on the skin surface pH, skin hydration and barrier integrity was assessed. Thirteen elderly females were involved in this home-in-use test. Increased baseline skin surface pH of the elderly normalizes to the physiological pH of 4.5 - 5.0 over 7 hours after single application of o/w-emulsions with a given pH of 3.5 or 4.0. A 4 week treatment employing the pH 4.0 skin care product improves the epidermal barrier integrity of the elderly significantly (p = 0.005). Reduction of the increased baseline skin surface pH of the elderly is accompanied by improved epidermal barrier integrity. Skin care products for the elderly have to be adjusted in the pH range of 3.5 to 4.0.
文摘目的研究中药挥发油透皮促渗苷类成分效果与对经皮水分散失量(Transepidermal water loss,TEWL)的相关性。方法采用HPLC法测定3种苷类成分(栀子苷、龙胆苦苷、芍药苷)的含量及油水分配系数,采用改良Franz扩散池法以离体SD大鼠腹部皮肤为透皮吸收屏障进行体外透皮实验,考察高良姜、干姜、薄荷、胡椒和吴茱萸5种中药挥发油对3种苷类成分的体外透皮促渗效果,采用经皮水分散失量评价5种中药挥发油对大鼠皮肤屏障功能的影响,通过相关性分析考察中药挥发油透皮促渗苷类成分效果与其对皮肤屏障功能影响之间的相关性。结果高良姜油、干姜油、薄荷油、胡椒油能促进3种苷类成分的透皮吸收,也能显著降低大鼠的皮肤屏障功能,相关性分析结果表明中药挥发油对苷类成分的透皮促渗效果与其对皮肤经皮水分散失量的影响显著相关。结论TEWL为中药挥发油透皮促渗剂的快速筛选提供了一种简便快捷的在体评价方法。
文摘目的:探讨含龙胆草提取物的液体敷料联合依巴斯汀治疗面部敏感性皮肤的疗效及其对皮肤生理功能的影响。方法:按照随机数字表法将2021年10月-2022年12月笔者医院收治的90例面部敏感性皮肤患者分为对照组与观察组,每组45例。对照组使用依巴斯汀治疗,观察组使用含龙胆草提取物的液体敷料联合依巴斯汀治疗,均连续治疗4周。比较两组的疗效,乳酸刺激试验指标,皮肤生理功能指标[经皮水分丢失量(Transepidermal water loss,TEWL)、皮脂含量、角质层含水量、皮肤pH值]及不良反应。结果:治疗结束后,观察组的总有效率(93.33%vs 71.11%)明显高于对照组(P<0.05);两组乳酸刺激试验指标,皮肤生理功能指标均治疗前明显改善(P<0.05);观察组乳酸刺痛评分明显低于对照组(P<0.05),乳酸刺痛感开始时间明显长于对照组(P<0.05);观察组TEWL、皮肤pH值明显低于对照组(P<0.05),皮脂含量、角质层含水量明显高于对照组(P<0.05)。两组均无严重不良反应发生。结论:含龙胆草提取物的液体敷料联合依巴斯汀能明显提高面部敏感性皮肤的临床疗效,明显改善皮肤生理功能及耐受性,安全性好。
