目的:分析新生儿重症监护病房(Neonatal Intensive Care Unit,NICU)早产儿接受发展性照顾对其生长发育与睡眠质量的影响。方法:选取2018年8月至2019年7月福建医科大学附属泉州市第一医院城东院区NICU病房收治的胎龄低于37周、出生体质...目的:分析新生儿重症监护病房(Neonatal Intensive Care Unit,NICU)早产儿接受发展性照顾对其生长发育与睡眠质量的影响。方法:选取2018年8月至2019年7月福建医科大学附属泉州市第一医院城东院区NICU病房收治的胎龄低于37周、出生体质量低于2.5 kg,适于胎龄的早产儿90例作为研究对象,按照随机数字表法分为对照组和观察组,每组45例。对照组给予常规护理,观察组给予发展性照顾,并比较2组早产儿摄入奶量、体质量增长、睡眠时间与住院时间及生长指标。结果:观察组摄入奶量与体质量增长均比对照组高,差异有统计学意义(P<0.05);观察组睡眠时间比对照组长,差异有统计学意义(P<0.05);观察组住院时间比对照组短,差异有统计学意义(P<0.05);观察组出生后30天早产儿身长与体质量比对照组高,差异有统计学意义(P<0.05)。结论:NICU早产儿接受发展性照顾,可延长睡眠时间,缩短住院时间,促进其生长发育。展开更多
Posttranscriptional mechanisms have a critical role in the overall outcome of gene expression. These mechanisms are especially relevant in protozoa from the genus Trypanosoma, which is composed by death threatening pa...Posttranscriptional mechanisms have a critical role in the overall outcome of gene expression. These mechanisms are especially relevant in protozoa from the genus Trypanosoma, which is composed by death threatening parasites affecting people in Sub-saharan Africa or in the Americas. In these parasites the classic view of regulation of transcription initiation to modulate the products of a given gene cannot be applied. This is due to the presence of transcription start sites that give rise to long polycistronic units that need to be processed costranscriptionally by trans-splicing and polyadenylation to give mature monocistronic mRNAs. Posttranscriptional mechanisms such as mRNA degradation and translational repression are responsible for the final synthesis of the required protein products. In this context, RNA-binding proteins(RBPs) in trypanosomes have a relevant role as modulators of mRNA abundance and translational repression by associating to the 3' untranslated regions in mRNA. Many different RBPs have been proposed to modulate cohorts of mRNAs in trypanosomes. However, the current understanding of their functions lacks a dynamic view on the different steps at which these RBPs are regulated. Here, we discuss different evidences to propose regulatory events for different RBPs in these parasites. These events vary from regulated developmental expression, to biogenesis of cytoplasmic ribonucleoprotein complexes in the nucleus, and condensation of RBPs and mRNA into large cytoplasmic granules. Finally, we discuss how newly identified posttranslational modifications of RBPs and mRNA metabolism-related proteins could have an enormous impact on the modulation of m RNA abundance. To understand these modifications is especially relevant in these parasites due to the fact that the enzymes involved could be interesting targets for drug therapy.展开更多
文摘目的:分析新生儿重症监护病房(Neonatal Intensive Care Unit,NICU)早产儿接受发展性照顾对其生长发育与睡眠质量的影响。方法:选取2018年8月至2019年7月福建医科大学附属泉州市第一医院城东院区NICU病房收治的胎龄低于37周、出生体质量低于2.5 kg,适于胎龄的早产儿90例作为研究对象,按照随机数字表法分为对照组和观察组,每组45例。对照组给予常规护理,观察组给予发展性照顾,并比较2组早产儿摄入奶量、体质量增长、睡眠时间与住院时间及生长指标。结果:观察组摄入奶量与体质量增长均比对照组高,差异有统计学意义(P<0.05);观察组睡眠时间比对照组长,差异有统计学意义(P<0.05);观察组住院时间比对照组短,差异有统计学意义(P<0.05);观察组出生后30天早产儿身长与体质量比对照组高,差异有统计学意义(P<0.05)。结论:NICU早产儿接受发展性照顾,可延长睡眠时间,缩短住院时间,促进其生长发育。
基金Supported by The Agencia Nacional de Promoción Científica y Tecnológica(ANPCyT)to Alejandro Cassola
文摘Posttranscriptional mechanisms have a critical role in the overall outcome of gene expression. These mechanisms are especially relevant in protozoa from the genus Trypanosoma, which is composed by death threatening parasites affecting people in Sub-saharan Africa or in the Americas. In these parasites the classic view of regulation of transcription initiation to modulate the products of a given gene cannot be applied. This is due to the presence of transcription start sites that give rise to long polycistronic units that need to be processed costranscriptionally by trans-splicing and polyadenylation to give mature monocistronic mRNAs. Posttranscriptional mechanisms such as mRNA degradation and translational repression are responsible for the final synthesis of the required protein products. In this context, RNA-binding proteins(RBPs) in trypanosomes have a relevant role as modulators of mRNA abundance and translational repression by associating to the 3' untranslated regions in mRNA. Many different RBPs have been proposed to modulate cohorts of mRNAs in trypanosomes. However, the current understanding of their functions lacks a dynamic view on the different steps at which these RBPs are regulated. Here, we discuss different evidences to propose regulatory events for different RBPs in these parasites. These events vary from regulated developmental expression, to biogenesis of cytoplasmic ribonucleoprotein complexes in the nucleus, and condensation of RBPs and mRNA into large cytoplasmic granules. Finally, we discuss how newly identified posttranslational modifications of RBPs and mRNA metabolism-related proteins could have an enormous impact on the modulation of m RNA abundance. To understand these modifications is especially relevant in these parasites due to the fact that the enzymes involved could be interesting targets for drug therapy.
