Developmental changes in intercellular junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods

Background： The intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different intercellular junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, intercellular junctions and voltage-gated potassium（Kv） channels in the intestine of piglets during the suckling and post-weaning periods.Results： Samples of the small intestine were obtained from 1-, 7-, 14-, and 21-d-old suckling piglets and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age. The results showed that the percentage of proliferating cell nuclear antigen（PCNA）-positive cells and alkaline phosphatase（AKP） activity, as well as the abundances of E-cadherin,occludin, and Kv1.5 m RNA and claudin-1, claudin-3, and occludin protein in the jejunum were increased from d 1to d 21 during the suckling period（P 〈 0.05）. Weaning induced decreases in the percentage of PCNA-positive cells,AKP activity and the abundances of E-cadherin, occludin and zonula occludens（ZO）-1 m RNA or protein in the jejunum on d 1, 3 and 5 post-weaning（P 〈 0.05）. There were lower abundances of E-cadherin, occludin and ZO-1m RNA as well as claudin-1, claudin-3 and ZO-1 protein in the jejunum of weanling piglets than in 21-d-old suckling piglets（P 〈 0.05）. The abundances of E-cadherin, occludin, ZO-1 and integrin m RNA were positively related to the percentage of PCNA-positive cells.Conclusion： Weaning at 14 d of age induced damage to the intestinal morphology and barrier. While there was an adaptive restoration on d 7 post-weaning, the measured values did not return to the pre-weaning levels, which reflected the impairment of intercellular junctions and Kv channels.

An Experimental Study on the Void Fraction for Gas-Liquid Two-Phase Flows in a Horizontal Pipe

The flow patterns and the void fraction related to a gas-liquid two-phase flow in a small channel are experimentally studied.The test channel is a transparent quartz glass circular channel with an inner diameter of 6.68 mm.The working fluids are air and water and their superficial velocities range from 0.014 to 8.127 m/s and from 0.0238 to 0.556 m/s,respectively.The void fraction is determined using the flow pattern images captured by a high-speed camera,while quick closing valves are used for verification.Four flow patterns are analyzed in experiments:slug flow,bubbly flow,annular flow and stratified flow.For intermittent flows(bubbly flow and slug flow),the cross-sectional void fraction is in a borderline condition while its probability distribution function(PDF)image displays a bimodal structure.For continuous flows(annular flow and stratified flow)the cross-sectional void fraction behaves as a fluctuating continuous curve while the(PDF)image displays a single peak structure.The volumetric void fraction data are also compared with available predictive formulas,and the results show that the agreement is very good.An effort is also provided to improve the so-called Gregory and Scott model using the available data.