Applicability of small resistance fastener on long-span continuous bridges of high-speed railway

Ballastless tracks have been widely applied in high-speed railway (HSR). The adaptability research between continuous welded rails (CWR) and long-span bridges of HSR is of great practical engineering significance. Based on the HSR long-span continuous bridges, the integrative spatial finite element model of track-bridge-pier-foundation system was established with the nonlinear spring element simulating the longitudinal resistance between track and bridge. Comparative study on the various additional longitudinal forces of CWR using the common fasteners and small resistance fasteners was carried out. Analysis results indicate that the additional expansion forces and additional rail-breaking forces in long-span ballastless continuous girders can be reduced evidently by 40% 50% after adopting small resistance fasteners, but lead to greater rail broken gap. The small resistance fasteners have little influence on the additional force only caused by vertical load, but can reduce the additional force caused by vertical load combined with braking load by over 10%. Besides, transient analysis method is proved to be more accurate and safe in calculating additional longitudinal forces when the train running or braking on the bridge, compared with the traditional static method.

Sedimentation motion of sand particles in moving water(Ⅰ):The resistance on a small sphere moving in non-uniform flow

In hydraulics,when we deal with the problem of sand particles moving relative to the surrounding water,Stokes'formula of resistance has usually been used to render the velocity of sedimentation of the particles.But such an approach has not been proved rigorously,and its accuracy must be carefully considered.In this paper,we discuss the problem of a sphere moving in a non-uniform flow field,on the basis of the fundamental theory of hydrodynamics.We introduce two assumptions:i)the diameter of the sphere is much smaller than the linear dimension of the flow field,and ii)the velocity of the sphere relative to the surrounding water is very small.Using these two assumptions,we solve the linearized Navier-Stokes equations and equations of continuity by the method of Laplace transform,and finally we obtain a formula for the resistance acting on a sphere moving in a non-uniform flow field.

Acceleration of DNA Replication of Klenow Fragment by Small Resisting Force

DNA polymerases are an essential class of enzymes or molecular motors that catalyze processive DNA syntheses during DNA replications. A critical issue for DNA polymerases is their molecular mechanism of processive DNA replication. We have proposed a model for chemomechanical coupling of DNA polymerases before, based on which the predicted results have been provided about the dependence of DNA replication velocity upon the external force on Klenow fragment of DNA polymerase I. Here, we performed single molecule measurements of the replication velocity of Klenow fragment under the external force by using magnetic tweezers. The single molecule data verified quantitatively the previous theoretical predictions, which is critical to the chemomechanical coupling mechanism of DNA polymerases. A prominent characteristic for the Klenow fragment is that the replication velocity is independent of the assisting force whereas the velocity increases largely with the increase of the resisting force,attains the maximum velocity at about 3.8 pN and then decreases with the further increase of the resisting force.

The function of small RNAs in plant biotic stress response

Small RNAs（s RNAs） play essential roles in plants upon biotic stress. Plants utilize RNA silencing machinery to facilitate pathogen-associated molecular pattern-triggered immunity and effector-triggered immunity to defend against pathogen attack or to facilitate defense against insect herbivores. Pathogens, on the other hand, are also able to generate effectors and s RNAs to counter the host immune response. The arms race between plants and pathogens/insect herbivores has triggered the evolution of s RNAs,RNA silencing machinery and pathogen effectors. A great number of studies have been performed to investigate the roles of s RNAs in plant defense, bringing in the opportunity to utilize s RNAs in plant protection. Transgenic plants with pathogen-derived resistance ability or transgenerational defense have been generated, which show promising potential as solutions for pathogen/insect herbivore problems in the field. Here we summarize the recent progress on the function of s RNAs in response to biotic stress, mainly in plant-pathogen/insect herbivore interaction,and the application of s RNAs in disease and insect herbivore control.

Bacillus cereus AR156 primes induced systemic resistance by suppressing miR825/825 and activating defense-related genes in Arabidopsis

Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance（ISR） is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that induces ISR in Arabidopsis against bacterial infection. Here,by comparing small RNA profiles of Pseudomonas syringae pv. tomato（Pst） DC3000-infected Arabidopsis with and without AR156 pretreatment, we identified a group of Arabidopsis micro RNAs（mi RNAs） that are differentially regulated by AR156 pretreatment. mi R825 and mi R825 are two mi RNA generated from a single mi RNA gene.Northern blot analysis indicated that they were significantly downregulated in Pst DC3000-infected plants pretreated with AR156, in contrast to the plants without AR156 pretreatment. mi R825 targets two ubiquitin-protein ligases,while mi R825 targets toll-interleukin-like receptor（TIR）-nucleotide binding site（NBS） and leucine-rich repeat（LRR）type resistance（R） genes. The expression of these target genes negatively correlated with the expression of mi R825 and mi R825. Moreover, transgenic plants showing reduced expression of mi R825 and mi R825 displayed enhanced resistance to Pst DC3000 infection, whereas transgenic plants overexpressing mi R825 and mi R825 were more susceptible. Taken together, our data indicates that Bacillus cereus AR156 pretreatment primes ISR to Pst infection by suppressing mi R825 and mi R825 and activating the defense related genes they targeted.