Background Brown adipose tissue(BAT)is known to be capable of non-shivering thermogenesis under cold stimulation,which is related to the mortality of animals.In the previous study,we observed that goat BAT is mainly l...Background Brown adipose tissue(BAT)is known to be capable of non-shivering thermogenesis under cold stimulation,which is related to the mortality of animals.In the previous study,we observed that goat BAT is mainly located around the kidney at birth,and changes to white adipose tissue(WAT)in the perirenal adipose tissue of goats within one month after birth.However,the regulatory factors underlying this change is remain unclear.In this study,we systematically studied the perirenal adipose tissue of goat kids in histological,cytological,and accompanying molecular level changes from 0 to 28 d after birth.Results Our study found a higher mortality rate in winter-born goat kids,with goat birthing data statistics.Then we used thermal imaging revealing high temperature in goat hips at postnatal 0 d and gradually decrease during 28 d.This is consistent with the region of perirenal BAT deposition and highlights its critical role in energy expenditure and body temperature regulation in goat kids.Additionally,we found a series of changes of BAT during the first 28 d after birth,such as whitening,larger lipid droplets,decreased mitochondrial numbers,and down-regulation of key thermogenesis-related genes(UCP1,DIO2,UCP2,CIDEA,PPARGC1a,C/EBPb,and C/EBPa).Then,we used RNA-seq found specific marker genes for goat adipose tissue and identified 12 new marker genes for BAT and 10 new marker genes for WAT of goats.Furthermore,12 candidate genes were found to potentially regulate goat BAT thermogenesis.The mechanism of the change of this biological phenomenon does not involve a large-scale death of brown adipocytes and subsequent proliferation of white adipocytes.While apoptosis may play a limited role,it is largely not critical in this transition process.Conclusions We concluded that perirenal BAT plays a crucial role in thermoregulation in newborn goat kids,with notable species differences in the expression of adipose tissue marker genes,and we highlighted some potential marker genes for goat BAT and WAT.Additionally,the change from BAT to WAT does not involve a large-scale death of brown adipocytes and subsequent proliferation of white adipocytes.展开更多
In recent years, the demand for goat products has been growing due to the fact that goat milk has a number of advantages over cow milk, for example, it is low in lactose, and is considered less allergenic and easier t...In recent years, the demand for goat products has been growing due to the fact that goat milk has a number of advantages over cow milk, for example, it is low in lactose, and is considered less allergenic and easier to digest. To increase production during both breeding and non-breeding seasons and reduce the price of dairy products, it is necessary to effectively use reproductive management and assisted reproductive technologies. In vitro embryo production makes it possible to obtain a large number of eggs from goats, which for some reason are unable to conceive, but have genetic value. Afterward in vitro produced embryos can be transferred into recipient goats of other less genetically valuable breeds, such as the Ukrainian local breed. Therefore, the aim of the present study was to investigate the effectiveness of transfers of in vitro produced embryos of Saanen goats into surrogate sires of the Ukrainian local breed in different seasons. All manipulations with animals were carried out following ethical standards (Strasbourg, 1986). Six Saanen goats were selected as the oocyte donors. After the hormonal stimulation oocytes were retrieved by laparoscopic ovum pick-up. In vitro produced embryos were transferred laparotomically into 24 recipients of Ukrainian local breed. Fifty days after embryo transfers, pregnancies were determined by ultrasound diagnostics. Although the embryo development rate in the breeding season was 20% higher than in the non-breeding season, there was no difference in pregnancy and kidding rates between seasons. In conclusion, the transfer of in vitro produced Saanen goat embryos to recipients of the Ukrainian local breed gives the opportunity to achieve pregnancy and kidding regardless of the breeding season, which will enable a faster and more efficient increase in the livestock of highly productive goats in Ukraine in the post-war period.展开更多
The caprice for the study came from an indigenous designed instructional board for teaching learners in nursery/primary schools (2 - 10 years) and the task whose overall topic is the reminiscence, retention of visuals...The caprice for the study came from an indigenous designed instructional board for teaching learners in nursery/primary schools (2 - 10 years) and the task whose overall topic is the reminiscence, retention of visuals aid use in the pedagogy. The study is to appraise the opinion of toddlers/teachers about the helpfulness of 3D-visuals (ergonomic board);the clarity of the intended functions of the 3D-visuals in the toddler’s lessons instruction and support from the producers in using them;and why visuals (conventional visual aids) in schoolroom instruction are misjudged/misinterpreted;their view about the functions in specific (conventional visuals) for the instruction pedagogy;and other sources of visuals provided other than the lesson’s instruction. Four nursery/primary schools participated in the study. Seventy-two (72) toddler’s/teachers participated in the study. The study examined using quantitative and qualitative approach for statistical analysis (using pie-chart and histogram). The findings suggested that the aspects of visuals items selected for comment and description are to some extent circumscribed by toddler’s learners’ linguistic resources. Understandably, the point made by researchers referred that visuals often fail to support learning as effectively as they might because they are not regarded as a full-fledged information mode that requires the same careful processing as verbal text, is borne out by the findings. Recommendation, the accountability is above all on producers of kid’s instructional materials: authors, editors, designers and illustrators of the materials. The implication of this is that toddlers’ instructional materials should, as in the case of illustrated books include both an overt explanation of the overall philosophy toward visuals and overview of their use.展开更多
目的:通过研究儿童呼吸和哮喘控制测试(test for respiratory and asthma control in kids,TRACK)和儿童哮喘控制测试(childhood asthma control test,C-ACT)与全球哮喘防治创议(global initiative for asthma,GINA)标准哮喘控制水平评...目的:通过研究儿童呼吸和哮喘控制测试(test for respiratory and asthma control in kids,TRACK)和儿童哮喘控制测试(childhood asthma control test,C-ACT)与全球哮喘防治创议(global initiative for asthma,GINA)标准哮喘控制水平评估的一致性、与哮喘儿童肺功能指标的相关性,探讨2种评分方法在儿童哮喘管理中的价值。方法:选择2020年8月至2022年3月重庆市妇幼保健院儿科门诊就诊的哮喘患儿135例为研究对象,用TRACK评分表和C-ACT评分表对相应年龄的患儿及其家长进行问卷调查,分析2种评分方法与GINA标准哮喘控制水平评估分级的一致性、与肺功能指标的相关性,并比较不同评分结果组肺功能指标差异。结果:TRACK评分、C-ACT评分与GINA哮喘控制水平分级一致性检验Kappa值分别为0.517和0.531,均显示一致性一般;TRACK评分与TPF%TE和VPF%VE存在显著的正相关,但TRACK评分、C-ACT评分与FEV1%均没有显著性相关;不同TRACK评分组哮喘患儿的FEV1%差异无统计学意义(F=2.054,P=0.134),但多重比较发现≥80分组与<60分组FEV1%的差异有统计学意义(P=0.048);不同TRACK评分组哮喘患儿的TPTEF/TE(%)(F=3.171,P=0.044)和VPEF/VE(%)(F=3.919,P=0.022)差异有统计学意义,进一步多重比较发现其差异主要来自于≥80分组与60~80分组,其中VPEF/VE(%)(P=0.017)的组间差异较TPTEF/TE(%)(P=0.030)更为明显;比较不同C-ACT评分组哮喘患儿的FEV1%差异无统计学意义(F=1.756,P=0.182)。结论:TRACK评分能更好反映儿童肺功能的差异,可作为5岁以下儿童哮喘管理的一个有效评估工具。展开更多
自杀是当前全球重大公共卫生问题之一,预防自杀已刻不容缓。经过30年的发展,加拿大自杀预防中心(Kids Help Phone)开展的青少年自杀预防教育在加拿大处于领导力地位。KHP下设咨询服务与项目部、收入发展部、财务与行政部、创新科技部、...自杀是当前全球重大公共卫生问题之一,预防自杀已刻不容缓。经过30年的发展,加拿大自杀预防中心(Kids Help Phone)开展的青少年自杀预防教育在加拿大处于领导力地位。KHP下设咨询服务与项目部、收入发展部、财务与行政部、创新科技部、新闻编辑室五个部门;借助这些部门,KHP聘用专业的雇员和志愿者,开发线上和线下实体项目、参与国家战略、加强与其他相关机构的联系,为青少年,尤其是大中小学生提供专业化的自杀预防服务和教育活动。KHP及其开展的青少年自杀预防教育对完善我国自杀预防机构、推进自杀预防教育专业化等,提供了借鉴意义。展开更多
This study aims to reveal the occurrence and origin of typical groundwater with high arsenic and fluoride concentrations in the loess area of the Guanzhong Basin—a Neogene faulted basin.Key findings are as follows:(1...This study aims to reveal the occurrence and origin of typical groundwater with high arsenic and fluoride concentrations in the loess area of the Guanzhong Basin—a Neogene faulted basin.Key findings are as follows:(1)Groundwater samples with high arsenic and fluoride concentrations collected from the loess area and the terraces of the Weihe River accounted for 26%and 30%,respectively,of the total samples,with primary hydrochemical type identified as HCO_(3)-Na.The karst and sand areas exhibit relatively high groundwater quality,serving as preferred sources for water supply.It is recommended that local governments fully harness groundwater in these areas;(2)groundwater with high arsenic and fluoride concentrations in the loess area and the alluvial plain of rivers in Dali County is primarily distributed within the Guanzhong Basin,which represents the drainage zone of groundwater;(3)arsenic and fluoride in groundwater originate principally from natural and anthropogenic sources;(4)the human health risk assessments reveal that long-term intake of groundwater with high arsenic and fluoride concentrations pose cancer or non-cancer risks,which are more serious to kids compared to adults.This study provides a theoretical basis for the prevention and treatment of groundwater with high arsenic and fluoride concentrations in loess areas.展开更多
目的探讨改良中文版儿童呼吸和哮喘控制测试(Test for Respiratory and Asthma Control in Kids Chinese Version,TRACK-C)在0~3岁哮喘婴幼儿不同患病时期的应用价值。方法选取2017年8月—2023年1月空军军医大学唐都医院小儿哮喘门诊中...目的探讨改良中文版儿童呼吸和哮喘控制测试(Test for Respiratory and Asthma Control in Kids Chinese Version,TRACK-C)在0~3岁哮喘婴幼儿不同患病时期的应用价值。方法选取2017年8月—2023年1月空军军医大学唐都医院小儿哮喘门诊中已建立标准化电子病历的203名0~3岁哮喘患儿作为哮喘组,同期100名健康儿童作为对照组。比较各组的TRACK-C评分、潮气肺功能、呼出气一氧化氮(Fractional Exhaled Nitric Oxide,FeNO)水平并进行相关性分析。结果哮喘组的FeNO、呼吸频率(Respiratory Rate,RR)和血液IgE水平高于对照组,达峰值时间比(Time to Peak Expirtory Flow/Time of Expiratory,TPTEF/TE)、达峰容积比(Volume to Peak Expiratory Flow/Volume of Expiratory,VPEF/VE)、公斤潮气量(Tidal Volume/kg,VT/KG)、TRACK-C评分水平低于对照组,差异有统计学意义(P均<0.05)。哮喘组急性期FeNO水平、RR水平高于缓解期,且TPTEF/TE、VPEF/VE、VT/KG、TRACK-C评分水平低于缓解期,差异有统计学意义(P均<0.05)。采用Pearson分析哮喘不同患病时期与各项观察指标的相关性,结果显示哮喘不同患病时期与TPTEF/TE、VPEF/VE、TRACK-C评分呈负相关(r=-0.468、-0.452、-0.289,P均<0.05)。结论TRACK-C评分可充分应用于0~3岁哮喘幼儿的病情评估,与潮气肺功能结合可以更好地对低龄患儿进行哮喘管理。展开更多
基金This work was financially supported by The National Key Research and Development Program of China(No.2022YFD1300202)The National Natural Science Foundation of China(No.32372834)+2 种基金Chongqing Modern Agricultural Industry Technology System(CQMAITS202313)the Collection,Utilization and Innovation of Germplasm Resources by Research Institutes and Enterprises of Chongqing,China(cqnyncw-kqlhtxm)the Chongqing Postgraduate Research Innovation Project(CYB22141).
文摘Background Brown adipose tissue(BAT)is known to be capable of non-shivering thermogenesis under cold stimulation,which is related to the mortality of animals.In the previous study,we observed that goat BAT is mainly located around the kidney at birth,and changes to white adipose tissue(WAT)in the perirenal adipose tissue of goats within one month after birth.However,the regulatory factors underlying this change is remain unclear.In this study,we systematically studied the perirenal adipose tissue of goat kids in histological,cytological,and accompanying molecular level changes from 0 to 28 d after birth.Results Our study found a higher mortality rate in winter-born goat kids,with goat birthing data statistics.Then we used thermal imaging revealing high temperature in goat hips at postnatal 0 d and gradually decrease during 28 d.This is consistent with the region of perirenal BAT deposition and highlights its critical role in energy expenditure and body temperature regulation in goat kids.Additionally,we found a series of changes of BAT during the first 28 d after birth,such as whitening,larger lipid droplets,decreased mitochondrial numbers,and down-regulation of key thermogenesis-related genes(UCP1,DIO2,UCP2,CIDEA,PPARGC1a,C/EBPb,and C/EBPa).Then,we used RNA-seq found specific marker genes for goat adipose tissue and identified 12 new marker genes for BAT and 10 new marker genes for WAT of goats.Furthermore,12 candidate genes were found to potentially regulate goat BAT thermogenesis.The mechanism of the change of this biological phenomenon does not involve a large-scale death of brown adipocytes and subsequent proliferation of white adipocytes.While apoptosis may play a limited role,it is largely not critical in this transition process.Conclusions We concluded that perirenal BAT plays a crucial role in thermoregulation in newborn goat kids,with notable species differences in the expression of adipose tissue marker genes,and we highlighted some potential marker genes for goat BAT and WAT.Additionally,the change from BAT to WAT does not involve a large-scale death of brown adipocytes and subsequent proliferation of white adipocytes.
文摘In recent years, the demand for goat products has been growing due to the fact that goat milk has a number of advantages over cow milk, for example, it is low in lactose, and is considered less allergenic and easier to digest. To increase production during both breeding and non-breeding seasons and reduce the price of dairy products, it is necessary to effectively use reproductive management and assisted reproductive technologies. In vitro embryo production makes it possible to obtain a large number of eggs from goats, which for some reason are unable to conceive, but have genetic value. Afterward in vitro produced embryos can be transferred into recipient goats of other less genetically valuable breeds, such as the Ukrainian local breed. Therefore, the aim of the present study was to investigate the effectiveness of transfers of in vitro produced embryos of Saanen goats into surrogate sires of the Ukrainian local breed in different seasons. All manipulations with animals were carried out following ethical standards (Strasbourg, 1986). Six Saanen goats were selected as the oocyte donors. After the hormonal stimulation oocytes were retrieved by laparoscopic ovum pick-up. In vitro produced embryos were transferred laparotomically into 24 recipients of Ukrainian local breed. Fifty days after embryo transfers, pregnancies were determined by ultrasound diagnostics. Although the embryo development rate in the breeding season was 20% higher than in the non-breeding season, there was no difference in pregnancy and kidding rates between seasons. In conclusion, the transfer of in vitro produced Saanen goat embryos to recipients of the Ukrainian local breed gives the opportunity to achieve pregnancy and kidding regardless of the breeding season, which will enable a faster and more efficient increase in the livestock of highly productive goats in Ukraine in the post-war period.
文摘The caprice for the study came from an indigenous designed instructional board for teaching learners in nursery/primary schools (2 - 10 years) and the task whose overall topic is the reminiscence, retention of visuals aid use in the pedagogy. The study is to appraise the opinion of toddlers/teachers about the helpfulness of 3D-visuals (ergonomic board);the clarity of the intended functions of the 3D-visuals in the toddler’s lessons instruction and support from the producers in using them;and why visuals (conventional visual aids) in schoolroom instruction are misjudged/misinterpreted;their view about the functions in specific (conventional visuals) for the instruction pedagogy;and other sources of visuals provided other than the lesson’s instruction. Four nursery/primary schools participated in the study. Seventy-two (72) toddler’s/teachers participated in the study. The study examined using quantitative and qualitative approach for statistical analysis (using pie-chart and histogram). The findings suggested that the aspects of visuals items selected for comment and description are to some extent circumscribed by toddler’s learners’ linguistic resources. Understandably, the point made by researchers referred that visuals often fail to support learning as effectively as they might because they are not regarded as a full-fledged information mode that requires the same careful processing as verbal text, is borne out by the findings. Recommendation, the accountability is above all on producers of kid’s instructional materials: authors, editors, designers and illustrators of the materials. The implication of this is that toddlers’ instructional materials should, as in the case of illustrated books include both an overt explanation of the overall philosophy toward visuals and overview of their use.
文摘目的:通过研究儿童呼吸和哮喘控制测试(test for respiratory and asthma control in kids,TRACK)和儿童哮喘控制测试(childhood asthma control test,C-ACT)与全球哮喘防治创议(global initiative for asthma,GINA)标准哮喘控制水平评估的一致性、与哮喘儿童肺功能指标的相关性,探讨2种评分方法在儿童哮喘管理中的价值。方法:选择2020年8月至2022年3月重庆市妇幼保健院儿科门诊就诊的哮喘患儿135例为研究对象,用TRACK评分表和C-ACT评分表对相应年龄的患儿及其家长进行问卷调查,分析2种评分方法与GINA标准哮喘控制水平评估分级的一致性、与肺功能指标的相关性,并比较不同评分结果组肺功能指标差异。结果:TRACK评分、C-ACT评分与GINA哮喘控制水平分级一致性检验Kappa值分别为0.517和0.531,均显示一致性一般;TRACK评分与TPF%TE和VPF%VE存在显著的正相关,但TRACK评分、C-ACT评分与FEV1%均没有显著性相关;不同TRACK评分组哮喘患儿的FEV1%差异无统计学意义(F=2.054,P=0.134),但多重比较发现≥80分组与<60分组FEV1%的差异有统计学意义(P=0.048);不同TRACK评分组哮喘患儿的TPTEF/TE(%)(F=3.171,P=0.044)和VPEF/VE(%)(F=3.919,P=0.022)差异有统计学意义,进一步多重比较发现其差异主要来自于≥80分组与60~80分组,其中VPEF/VE(%)(P=0.017)的组间差异较TPTEF/TE(%)(P=0.030)更为明显;比较不同C-ACT评分组哮喘患儿的FEV1%差异无统计学意义(F=1.756,P=0.182)。结论:TRACK评分能更好反映儿童肺功能的差异,可作为5岁以下儿童哮喘管理的一个有效评估工具。
文摘自杀是当前全球重大公共卫生问题之一,预防自杀已刻不容缓。经过30年的发展,加拿大自杀预防中心(Kids Help Phone)开展的青少年自杀预防教育在加拿大处于领导力地位。KHP下设咨询服务与项目部、收入发展部、财务与行政部、创新科技部、新闻编辑室五个部门;借助这些部门,KHP聘用专业的雇员和志愿者,开发线上和线下实体项目、参与国家战略、加强与其他相关机构的联系,为青少年,尤其是大中小学生提供专业化的自杀预防服务和教育活动。KHP及其开展的青少年自杀预防教育对完善我国自杀预防机构、推进自杀预防教育专业化等,提供了借鉴意义。
基金funded by the ministry-province cooperation-based pilot project entitled A Technological System for Ecological Remediation Evaluation of Open-Pit Mines initiated by the Ministry of Natural Resources in 2023(2023-03)survey projects of the Land and Resources Investigation Program([2023]06-03-04,1212010634713)a key R&D projects of Shaanxi Province in 2023(2023ZDLSF-63)。
文摘This study aims to reveal the occurrence and origin of typical groundwater with high arsenic and fluoride concentrations in the loess area of the Guanzhong Basin—a Neogene faulted basin.Key findings are as follows:(1)Groundwater samples with high arsenic and fluoride concentrations collected from the loess area and the terraces of the Weihe River accounted for 26%and 30%,respectively,of the total samples,with primary hydrochemical type identified as HCO_(3)-Na.The karst and sand areas exhibit relatively high groundwater quality,serving as preferred sources for water supply.It is recommended that local governments fully harness groundwater in these areas;(2)groundwater with high arsenic and fluoride concentrations in the loess area and the alluvial plain of rivers in Dali County is primarily distributed within the Guanzhong Basin,which represents the drainage zone of groundwater;(3)arsenic and fluoride in groundwater originate principally from natural and anthropogenic sources;(4)the human health risk assessments reveal that long-term intake of groundwater with high arsenic and fluoride concentrations pose cancer or non-cancer risks,which are more serious to kids compared to adults.This study provides a theoretical basis for the prevention and treatment of groundwater with high arsenic and fluoride concentrations in loess areas.
文摘目的探讨改良中文版儿童呼吸和哮喘控制测试(Test for Respiratory and Asthma Control in Kids Chinese Version,TRACK-C)在0~3岁哮喘婴幼儿不同患病时期的应用价值。方法选取2017年8月—2023年1月空军军医大学唐都医院小儿哮喘门诊中已建立标准化电子病历的203名0~3岁哮喘患儿作为哮喘组,同期100名健康儿童作为对照组。比较各组的TRACK-C评分、潮气肺功能、呼出气一氧化氮(Fractional Exhaled Nitric Oxide,FeNO)水平并进行相关性分析。结果哮喘组的FeNO、呼吸频率(Respiratory Rate,RR)和血液IgE水平高于对照组,达峰值时间比(Time to Peak Expirtory Flow/Time of Expiratory,TPTEF/TE)、达峰容积比(Volume to Peak Expiratory Flow/Volume of Expiratory,VPEF/VE)、公斤潮气量(Tidal Volume/kg,VT/KG)、TRACK-C评分水平低于对照组,差异有统计学意义(P均<0.05)。哮喘组急性期FeNO水平、RR水平高于缓解期,且TPTEF/TE、VPEF/VE、VT/KG、TRACK-C评分水平低于缓解期,差异有统计学意义(P均<0.05)。采用Pearson分析哮喘不同患病时期与各项观察指标的相关性,结果显示哮喘不同患病时期与TPTEF/TE、VPEF/VE、TRACK-C评分呈负相关(r=-0.468、-0.452、-0.289,P均<0.05)。结论TRACK-C评分可充分应用于0~3岁哮喘幼儿的病情评估,与潮气肺功能结合可以更好地对低龄患儿进行哮喘管理。
