Clinical significance of serum oxidative stress and serum uric acid levels before surgery for hepatitis B-related liver cancer

BACKGROUND The incidence and mortality of liver cancer are among the highest of all malignant tumors in China.The high recurrence rate after conventional hepatectomy is worrying.There is a lack of effective prognostic indicators for liver cancer.AIM To explore the clinical significance of preoperative serum oxidative stress and serum uric acid(UA)levels in hepatitis B-related liver cancer.METHODS The medical records of 110 hepatitis B-related liver cancer patients who under-went hepatectomy in Gansu Provincial Hospital were retrospectively analyzed.Recurrence in patients within 3 years after surgery was determined.The logistic regression model and Pearson or Spearman correlation were used to analyze the correlation between oxidative stress level and UA,and the recurrence of hepatitis B-related liver cancer.RESULTS Compared with the non-recurrence group,the levels of superoxide dismutase(SOD)and glutathione(GSH)in the recurrence group were lower and the levels of malondialdehyde(MDA)and UA were higher(all P<0.05).UA,SOD,MDA,and GSH were risk factors for postoperative recurrence in hepatitis B-related liver cancer patients(P<0.05).UA was positively correlated with MDA(r=0.395,P<0.001)and negatively correlated with GSH(r=-0.204,P=0.032).The area under the receiver operating characteristic curve(AUC)of SOD,MDA,GSH,and UA in predicting the prognosis was 0.276,0.910,0.199,and 0.784,respectively(all P<0.001).CONCLUSION The preoperative serum SOD,GSH,MDA,and UA levels had significant predictive effects on postoperative recurrence of hepatitis B-related liver cancer.

The effect of plasma uric acid on oxidative stress in ankylosing spondylitis by Keap1-Nrf2 signaling pathway

Objective:To investigate the mechanism of serum uric acid in Ankylosing spondylitis(AS)through the Kelch-like ECH-Associating protein 1(Keap1)-nuclear factor erythroid 2 related factor 2(Nrf2)signaling pathway.Methods:A total of 60 AS patients in our hospital from March 2018 to October 2019 were recruited and divided into the active group(>4 points,26 cases)and the inactive group(≤4 points,34 cases)according to the Bath Ankylosing Spondylitis Disease Activity Index(BASDAI).Keap1,Nrf2,catalase(CAT),superoxide dismutase(SOD),Malondialdehyde(MDA),reactive nitrogen species(RNS),reactive oxygen species(ROS)were detected by ELISA;furthermore,erythrocyte sedimentation rate(ESR),Uric acid(UA)and C-reactive protein(CRP)were tested.The relationship among UA,BASDAI and oxidative stress indicators were analyzed.Results:The expression levels of ESR,CRP,UA,ROS,RNS,MDA,and Keap1 in the active group were significantly higher than those in the inactive group(all P<0.001);the levels of Nrf2,CAT,and SOD in the active group were higher than those in the inactive group,markedly reduced(all P<0.001)in the inactive group.The Pearson's correlation coefficient showed that ROS,RNS,MDA,and Keap1 were notably positively correlated with blood UA and BASDAI in the active group with AS patients;while Nrf2,CAT,and SOD were negatively correlated with blood UA and BASDAI.Moreover,blood UA and BASDAI were found to be moderately positively correlated.Conclusion:The results in the study demonstrate that the UA level in blood is related to the AS disease activity,blood UA exerts the pro-inflammatory effects,increasing oxidative stress injury and reducing antioxidant capacity,possibly by activating the Keap1-Nrf2 pathway.

Correlation of serum uric acid level with neurotrophy, nerve injury and systemic oxidative stress response in patients with Parkinson's disease

Objective:To investigate the correlation of serum uric acid level with neurotrophy, nerve injury and systemic oxidative stress response in patients with Parkinson's disease.Methods:A total of 60 patients with Parkinson's disease who were diagnosed and treated in our hospital between July 2014 and February 2018 were enrolled in Parkinson's disease group, and 100 healthy elderly people who received physical examination in our hospital during the same period were enrolled in normal control group. The differences in serum uric acid levels as well as the contents of indexes related to neurotrophy, nerve injury and oxidative stress were compared between the two groups, and Pearson test was used to evaluate the correlation of serum uric acid level with indexes related to neurotrophy, nerve injury and oxidative stress in patients with Parkinson's disease.Results:Serum uric acid level of PD group was lower than that of normal control group;serum neurotrophy indexes BDNF, GDNF and NGF contents were lower than those of normal control group;serum nerve injury indexes YKL-40 and S-100B contents were higher than those of normal control group whereas IGF-1 content was lower than that of normal control group;serum oxidative stress indexes GPX and CAT contents were lower than those of normal control group whereas MDA and LHP contents were higher than those of normal control group. Correlation analysis confirmed that the serum uric acid level of patients with Parkinson's disease was directly correlated to the indexes related to neurotrophy, nerve injury and oxidative stress.Conclusion:The abnormal decrease of serum uric acid level in patients with Parkinson's disease is directly correlated with the neurotrophy, nerve injury, oxidative stress response, and so on.

Protective Effects of α-Lipoic Acid on Vascular Oxidative Stress in Rats with Hyperuricemia

The aim of the present study was to observe the protective effects of α-lipoic acid(ALA)on vascular injury in rats with hyperuricemia(HUA).The ALA treatment groups(10,30 and 90 mg/kg,respectively)were administered with ALA via gavage for 2 weeks.Subsequently,the levels of blood urea nitrogen(BUN),creatinine(CREA),uric acid(UA),total cholesterol(TC),high density lipoprotein-C(HDL-C)and low density lipoprotein-C(LDL-C)were measured;the activities of glutathione peroxidase(GSH-Px),catalase(CAT),malonaldehyde(MDA),superoxide dismutase(SOD)and xanthine oxidase(XOD)were also determined.The thoracic aorta of rats in each experimental group was observed under a light microscope;ultrastructural analysis was performed.SOD and CAT protein contents were investigated by Western blotting.The results revealed that:i)Compared with the model group,the levels of UA were decreased in the ALA groups and the levels of BUN,CREA,TC,and LDL-C decreased in the 30 and 90 mg/kg ALA groups(P<0.05);ii)compared with the model group,the activities of GSH-Px,SOD and XOD were increased and the levels of MDA were reduced in the 90 mg/kg ALA group (P<0.05);and iii)in the model and 10 mg/kg ALA groups,edema and shedding were observed in endothelial cells.Compared with the model and 10 mg/kg ALA groups,the 30 and 90 mg/kg ALA groups exhibited fewer swollen endothelial cells.In summary,the results of the present study indicated that HUA resulted in vascular oxidative stress injury and decreased the activity of antioxidative enzymes,which leads to endothelial cell damage and vascular lesions.ALA may serve as a therapeutic agent for the treatment of HUA-induced endothelial dysfunction.

Evaluation of oxidative and nitrosative stress in relapsing remitting multiple sclerosis

Multiple sclerosis (MS) is a chronic autoimmune disorder affecting the central nervous system (CNS) through demyelination and neurodegeneration. Several lines of evidence support a role for oxidative and nitrative stress (OS and NS) in pathogenesis of multiple sclerosis. The mechanism of influence of OS and NS on blood-brainbarrier (BBB) has critical importance for evaluating antioxidant therapies. As far as we know, markers of oxidative and nitrative stress in MS patients have been investigated independently for their relationship with the state of the blood-brain-barrier. Blood plasma samples of 58 patients with relapse-remitting MS (RRMS) with normal (Group A, n = 48, 36.2 ± 10.5 years) and damaged BBB (Group B, n = 10, 38.2 ± 11.2 years) and of 44 healthy controls (39.2 ± 14.9 years) were analyzed. TAS (total antioxidant plasma status), lipoperoxides, protein carbonyls, 3-nitrotyrosine and uric acid were evaluated in each group. Our results confirmed decreased TAS (Group A: 1.35 ± 0.55 mmol/l, P e level of lipoperoxidation positively correlated with the state of BBB (P of protein’s carbonyls (A: 0.48 ± 0.11 nmol/mg protein, P nmol/l, P ed damage to plasma proteins, what was confirmed by their positive mutual correlation (P The level of uric acid was physiological and correlated negatively with protein’s carbonyls (P 0.05) while there was no significant relationship with 3-nitotyrosine. The results suggest the role of this antioxidant in the protection of the proteins against OS what was confirmed by its positive correlation with TAS

龙胆苦苷对果糖诱导小鼠高尿酸血症的作用

目的:研究龙胆苦苷对果糖诱导小鼠高尿酸血症的治疗作用。方法:昆明种小鼠连续饮用10%果糖溶液12周以制备高尿酸症小鼠模型,从第9周开始,别嘌呤醇组,龙胆苦苷高、中、低剂量组(80 mg/kg、40 mg/kg、20 mg/kg-)每天灌胃给药1次,共给药4周。末次给药后取血,检测血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、尿酸(UA)、肌酐(Cr)和血尿素氮(BUN)水平,苏木精-伊红(HE)染色观察肝、肾组织的变化;酶联免疫吸附试验法检测肾组织中超氧化物歧化酶(SOD),丙二醛(MDA),肿瘤坏死因子α(TNF-α)和白细胞介素-6(IL-6)水平,比色法和蛋白质免疫印迹法检测肝组织中黄嘌呤氧化酶(XOD)活性及蛋白表达水平。结果:与模型组比较,龙胆苦苷各给药组小鼠血清GPT、GOT、UA、Cra和BUN水平显著降低(P<0.01),肝、肾病理学变化有所改善;肾脏SOD水平显著升高(P<0.01),MDA、TNF-α和IL-6水平显著降低(P<0.01);同时肝脏XOD的活性及蛋白表达水平显著下调(P<0.01或P<0.05)。结论:龙胆苦苷通过降低XOD活性和表达,降低尿酸水平,进而减轻肾脏氧化应激和炎症水平,达到肾脏保护作用。

非布司他联合吲哚美辛对痛风性关节炎患者血尿酸、疼痛程度及氧化应激水平的影响

目的探究非布司他联合吲哚美辛对痛风性关节炎患者血尿酸、疼痛程度及氧化应激水平的影响。方法选取2022年1月—2023年5月新疆维吾尔自治区人民医院内分泌与代谢病科收治的痛风性关节炎患者86例,按随机数字表法分为对照组(n=43)和观察组(n=43)。对照组患者接受非布司他片口服,观察组患者在此基础上联合吲哚美辛片口服,2组均持续治疗1个月。评价2组患者的临床疗效,通过视觉模拟评分法(VAS)评估患者治疗前、治疗2周及1个月时关节疼痛程度;比较2组患者治疗前后肾功能指标[血清肌酐(SCr)、血尿酸(UA)、尿素氮(BUN)]、血清炎性指标[白介素1β(IL-1β)、核苷酸寡聚化结构域样受体蛋白3(NALP3)、肿瘤坏死因子α(TNF-α)]、氧化应激指标[血清谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)及丙二醛(MDA)]及不良事件发生率。结果观察组治疗总有效率高于对照组(88.37%vs.69.77%,χ^(2)/P=4.497/0.034);治疗2周后,2组患者VAS评分均低于治疗前,且观察组低于对照组(t/P=5.324/<0.001);治疗1个月后2组患者血清UA及SCr水平较治疗前降低,且观察组低于对照组(t/P=3.081/0.003、2.692/0.009);2组血清IL-1β及NALP3水平较治疗前降低,且观察组低于对照组(t/P=5.587/<0.001、2.465/0.016);2组血清GSH-Px及SOD水平较治疗前升高,血清MDA降低,且观察组高于/低于对照组患者(t/P=3.709/<0.001、3.655/<0.001、3.653/<0.001);观察组疼痛消失时间短于对照组(t/P=4.025/<0.001);观察组药物相关不良事件总发生率低于对照组,但差异无统计学意义(11.63%vs.18.60%,χ^(2)/P=0.816/0.366)。结论非布司他联合吲哚美辛对痛风性关节炎患者的肾功能改善具有更显著的效果,同时可有效降低患者炎性反应及氧化应激水平,有利于提高患者临床疗效且具有较好的临床安全性。

薯蓣皂苷通过GSK3β/Nrf2/HO-1通路改善尿酸诱导的HK-2细胞氧化应激损伤的作用及机制研究

目的探讨薯蓣皂苷(dioscin)对尿酸(uric acid,UA)诱导的人肾小管上皮细胞(HK-2)氧化应激损伤的影响及分子机制。方法将HK-2细胞分为正常组、模型组(尿酸刺激造模)、条件对照组(尿酸+DMSO)和薯蓣皂苷组(尿酸+薯蓣皂苷)。通过尿酸诱导HK-2细胞氧化应激损伤模型;采用CCK-8法检测细胞活力,流式细胞技术检测细胞活性氧(ROS)水平,Real-time PCR法检测糖原合成激酶3(GSK3β)、核转录因子红系2相关因子2(Nrf2)和血红素加氧酶1(HO-1)在mRNA水平的表达,Western Blot法检测GSK3β、磷酸化糖原合成激酶3(p-GSK3β)、Nrf2及HO-1在蛋白水平的表达。结果经尿酸刺激后,HK-2细胞的活力明显下降,ROS水平明显升高(均P<0.001)。经薯蓣皂苷治疗后,HK-2细胞的活力增加,ROS水平明显降低(均P<0.001)。在蛋白及mRNA水平上,尿酸刺激后Nrf2和HO-1的表达均下降,薯蓣皂苷干预后Nrf2和HO-1表达均明显增加(均P<0.001)。在蛋白水平上,模型组细胞p-GSK3β/GSK3β比值较正常组明显下降,经薯蓣皂苷干预后p-GSK3β/GSK3β比值升高(均P<0.001)。结论薯蓣皂苷可能是通过促进GSK3β的磷酸化,激活Nrf2/HO-1通路,从而缓解尿酸诱导的HK-2细胞氧化应激损伤。

非酒精性脂肪性肝病患者血尿酸水平与心房颤动的相关性研究

目的探讨非酒精性脂肪性肝病(NAFLD)患者血尿酸水平与心房颤动的相关性。方法回顾性选取2020年1月至2022年12月于山西医科大学第二医院诊断为NAFLD的患者565例,根据是否合并心房颤动将其分为心房颤动组(224例)和非心房颤动组(341例)。分析2组患者的临床资料。多因素Logistic回归分析用于确定NAFLD患者心房颤动发生的相关因素。使用趋势性检验分析血尿酸水平与心房颤动之间的线性趋势。构建列线图模型预测心房颤动的发生风险,绘制校准曲线来评价列线图的准确性。最后绘制受试者工作特征(ROC)曲线,评估血尿酸和列线图模型对NAFLD患者心房颤动发生的预测价值。结果多因素Logistic回归结果显示,年龄(比值比=1.096,95%置信区间:1.071~1.121,P<0.001)、血肌酐(比值比=1.021,95%置信区间:1.003~1.039,P=0.025)及血尿酸(比值比=1.008,95%置信区间:1.005~1.011,P<0.001)是NAFLD患者心房颤动发生的独立危险因素,高密度脂蛋白(P=0.010)是独立保护因素。心房颤动的风险随着血尿酸水平的升高而增加(趋势性检验P<0.001)。列线图模型预测心房颤动的C指数为0.827(95%置信区间:0.793~0.861),Hosmer-Lemeshow检验和校准图均显示该模型的校准度良好。ROC分析显示列线图模型的曲线下面积为0.827,优于血尿酸的0.683。结论血尿酸水平升高是NAFLD患者心房颤动发生的独立危险因素。

血尿酸与慢性阻塞性肺疾病相关性研究进展

慢性阻塞性肺疾病(COPD)是一种常见的慢性呼吸系统疾病。其发生发展与炎症反应、氧化应激密切相关。而血清尿酸通过诱导内源性免疫细胞来增加炎症反应。尿酸在缺氧状态下会进一步升高,促进炎症及氧化应激反应,影响肺组织通气功能。血清尿酸水平可以作为评估患者病情严重程度、治疗效果及预后的重要指标,但其生物学机制仍需进一步研究证实。就此,本文综述血清尿酸与COPD疾病的关联。

Intestinal parameters of oxidative imbalance in celiac adults with extraintestinal manifestations

AIM To evaluate selected intestinal parameters of oxidative stress, and antioxidant capacity in adult celiac disease patients with extraintestinal manifestations.METHODS The study involved 85 adult patients divided into the following subgroups:(1) patients with newly diagnosed celiac disease(CD)(n = 7);(2) celiac patients not adhering to a gluten-free diet(GFD)(n = 22);(3) patients with CD on the GFD(n = 31); and(4) patients with functional disorders of the gastrointestinal tract, serving as controls(n = 25). Celiac patients presented with non-classic symptoms or extraintestinal manifestations. Standard blood tests including serum antioxidant levels(uric acid, bilirubin, and vitamin D), celiac antibody levels, and histopathological status of duodenal biopsy specimens have been determined. The expression of m RNA for tumor necrosis factor α(TNF-α), interleukin 1β(IL-1β), interleukin 10(IL-10), superoxide dismutase(SOD), heat-shock protein 70(HSP-70), hypoxia-inducible factor 1(HIF-1α), and BAX in the duodenal mucosa of patients was analyzed by reverse transcriptase-polymerase chain reaction. RESULTS The mean plasma uric acid level in patients with active CD(newly diagnosed and nonadherent patients) and treated celiac patients was significantly higher than in controls(260.17 ± 53.65 vs 190.8 ± 22.98, P < 0.001, and 261.7 ± 51.79 vs 190.8 ± 22.98, P < 0.001, respectively). The mean bilirubin concentration in active and treated celiac patients was significantly lower than in controls(8.23 ± 5.04 vs 10.48 ± 4.08, P < 0.05 and 8.06 ± 3.31 vs 10.48 ± 4.08, P < 0.05, respectively). The mean plasma vitamin D level was significantly lower in active celiac patients than in treated celiac patients and controls(19.37 ± 9.03 vs 25.15 ± 11.2, P < 0.05 and 19.37 ± 9.03 vs 29.67 ± 5.12, P < 0.001, respectively). The expression of TNF-α, IL-10, and HSP-70 m RNAs was significantly elevated in the celiac groups regardless of the diet when compared with controls. Patients on the GFD presented a significantly lower m RNA expression of TNF-α and IL-10 than in newly diagnosed and nonadherent patients(P < 0.05). The expression of SOD m RNA was significantly elevated in celiac patients compared with controls(P < 0.05), with a significant difference between treated and untreated patients(P < 0.05). The expression of HIF-1α m RNA and BAX m RNA was significantly higher in patients with active CD compared with controls and patients on GFD, while no difference was observed between the latter two groups. CONCLUSION Increased intestinal expression of HSP-70 despite GFD indicates that GFD only partially reduced oxidative stress. CD patients exhibited an oxidative imbalance and inflammatory response despite GFD. Uric acid may act as an important antioxidant in CD.

降尿酸方对尿酸性肾病血管保护及氧化应激的影响

目的观察降尿酸方对尿酸性肾病患者血管保护及氧化应激反应的影响。方法随机选取2021年1月1日-2021年12月31日期间收治的80例尿酸性肾病患者,采用随机数表法分为西医组(40例,单纯西医治疗)与中西医结合组(40例,西医治疗加降尿酸方),对比2组治疗总有效率、血管内皮功能、肾功能、氧化应激功能、不良反应。结果中西医结合组整体治疗效果优于西医组,差异有统计学意义(Z=2.056,P=0.04);治疗结束1周,与西医组相比,中西医结合组患者血清丙二醛(MDA)、晚期蛋白氧化产物(AOPP)、血清内皮素-1(ET-1)、血尿酸(SUA)、血肌酐(SCr)、血尿素氮(BUN)较低,血清超氧化物歧化酶(SOD)、总抗氧化能力(TAOC)、一氧化氮(NO)、内生肌酐清除率(CCr)水平较高(P均<0.001);2组患者不良反应总发生率对比,P>0.05。结论降尿酸方治疗尿酸性肾病患者显著,利于改善血管内皮功能及肾功能,抑制氧化应激反应,且安全性理想。

岩藻多糖对高尿酸诱导HepG2细胞凋亡影响及机制

目的探讨岩藻多糖(Fucoidan)对高浓度尿酸(UA)诱导的人肝癌细胞(HepG2)线粒体凋亡的影响及机制。方法HepG2细胞随机分为对照组(加无药物培养液)、UA模型组(加入0.2 g/L UA溶液)、白黎芦醇组(Res组,用1μmol/L白藜芦醇溶液预处理24 h后加入0.2 g/L UA溶液)、F1组(加入0.2 g/L UA+20 mg/L Fucoidan溶液处理)、F2组(加入0.2 g/L UA+40 mg/L Fucoidan溶液处理)、F1+EX527组(加入0.2 g/L UA+20 mg/L Fucoidan+1μmol/L EX527溶液处理)、F2+EX527组(加入0.2 g/L UA+40 mg/L Fucoidan+1μmol/L EX527溶液处理)。各组加入相应药物处理24 h后,应用CCK8实验检测HepG2细胞活力,ELISA实验检测细胞上清液丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平及细胞内超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)和丙二醛(MDA)水平;荧光显微镜检测活性氧(ROS)和线粒体膜电位,流式细胞仪测定细胞凋亡率,Western blot方法检测Sirt1蛋白表达。结果与对照组相比,UA模型组HepG2细胞活力降低(F=295.200,P<0.01),细胞上清液ALT和AST水平升高(F=204.300、9.511,P<0.01),细胞内SOD和GSH水平降低(F=47.880、8.261,P<0.01),MDA含量增加(F=132.400,P<0.01),ROS生成增加(F=23.720,P<0.05),Sirt1蛋白表达下调(F=64.520,P<0.01),细胞凋亡率明显升高(F=19.200,P<0.01);与UA模型组相比,Res组、F1组和F2组HepG2细胞生存率增加,ALT和AST水平降低,细胞内SOD和GSH水平增加,MDA含量降低,差异均有显著性(P<0.01);与UA模型组相比,F2组细胞内ROS生成减少,线粒体膜电位下降,Sirt1蛋白表达上调,细胞凋亡率下降,差异有显著性(P<0.05)。加入Sirt1抑制剂EX527能显著逆转Fucoidan的抗氧化和抗凋亡作用,差异有显著性(P<0.05)。结论Fucoidan通过上调Sirt1蛋白表达抑制线粒体氧化应激,逆转UA诱导的HepG2细胞线粒体凋亡。

中药排石汤治疗泌尿系结石的研究现状

泌尿系结石作为泌尿外科常见的疾病,目前以手术治疗为主,药物治疗为辅。中医药在治疗泌尿系结石方面有悠久历史,其中排石汤是治疗泌尿系结石常用的中医汤剂,具有利尿通淋、清热除湿等功效;此外,排石汤还能够降尿酸、抗氧化、减轻炎症反应。近年关于排石汤治疗泌尿系结石的临床研究不断增多,但研究结果存在差异,其具体发病机制仍有待进一步研究,深入研究排石汤在泌尿系结石中的作用及机制,可为泌尿系结石治疗药物的选择提供参考。

普罗布考对脑梗死患者氧化应激水平、尿酸及临床预后的影响

目的研究普罗布考对脑梗死患者氧化应激水平、尿酸及临床预后的影响。方法将160例缺血性脑卒中患者随机分为实验组和对照组两组,除基础治疗外,实验组予以阿托伐他汀钙片联合普罗布考治疗,对照组予阿托伐他汀治疗。于入院第1天和第14天分别测定缺血性卒中患者的超氧化物歧化酶(SOD)、总抗氧化能力(TAC)、丙二醛(MDA)及尿酸水平,于发病第90天应用改良Rankin量表评价其神经功能。分析普罗布考与氧化应激水平、尿酸及临床预后的关系。结果两组卒中进展比例比较,差异有统计学意义(P<0.05),实验组低于对照组。两组第90天时的神经功能比较,差异有统计学意义(P<0.05),实验组优于对照组。通过比较两组间氧化应激水平发现,普罗布考可以降低脑梗死患者的氧化应激及尿酸水平,进一步改善患者的神经功能。结论普罗布考可以通过降低脑梗死患者的氧化应激及尿酸水平,改善患者预后。

吸烟对血浆尿酸水平的影响

目的测定吸烟对血浆尿酸的影响及其与烟草代谢物的关系。方法选取健康体检者300例,其中吸烟者162例(男性145例,女性17例),不吸烟者138例(男性62例,女性76例),测定血浆尿酸、肌酐以及尿可替宁水平。结果吸烟者血浆尿酸水平较不吸烟者明显降低,差异有统计学意义。血浆尿酸水平与吸烟量、吸烟时间呈负相关。结论在排除影响血浆尿酸的因素以后,吸烟者尿酸水平较不吸烟者明显降低,且这种降低与尿酸内源性生成减少有关,是吸烟促发人体应激反应作用的结果。由于血浆尿酸测定方法简单,而且能反映人体的抗氧化物水平,因此它有可能作为了解吸烟应激状态的常规测定指标。

营养状况及血清尿酸水平与慢性阻塞性肺疾病的关系

目的测定慢性阻塞性肺疾病(COPD)患者实际体重与标准体重的百分比(IBM%)及血清尿酸 (UA)水平,以探讨其与COPD严重程度的关系。方法测定187例稳定期COPD患者的IBM%、血清UA、肺功能[第1秒用力呼气容积的实测值占预计值的百分比(FEV1%)、第1秒用力呼气容积占用力肺活量的百分比 (FEV1/FVC)、用力呼气中期流速(MMEF)、最大呼气峰流速(PEF)、75%肺活量时的最大呼气流速(FEF25)、 50%肺活量时的最大呼气流速(FEF50)]及心电图,根据气流受限程度分为ⅡA、ⅡB、Ⅲ级COPD,根据心电图检查结果分成伴肺源性心脏病(肺心病)组和不伴肺心病组。结果ⅡA级COPD组的血清IBM%为1．03±0．15, ⅡB级COPD组为0．95±0．15,Ⅲ级COPD组为0．93±0．14,ⅡA级COPD组与ⅡB级COPD组和Ⅲ级COPD 组的差异有显著性(P<0．05和<0．01)。ⅡA级COPD组血清UA水平为(380．54±77．62)μmol／L,ⅡB级 COPD组为(356．32±65．33)μmol/L,Ⅲ级COPD组为(335．06±57．97)μmol／L,各组间比较差异均有显著性 (P值均<0．05)。伴肺心病组的血清IBM%为0．92±0．15,不伴肺心病组为1．01±0．14,两组间差异有显著性 (P<0．05)。伴肺心病组的血清UA为(340．36±65．46)μmol／L,不伴肺心病组为(366．28±68．78)μmol／L,两组间差异有显著性(P<0．01)。在控制年龄、营养等因素后,UA与FEV1%呈显著正相关(标准化回归系数= 0．217,P<0．01)。结论营养不良是COPD独立的预后因子;血清UA降低是COPD的独立危险因子之一。

帕金森病患者血尿酸和25-羟维生素D_3水平的观察研究

目的探讨血尿酸和25-羟维生素D3与帕金森病的关系。方法选取2014年8月~2016年8月在南京医科大学附属淮安第一医院神经内科住院的帕金森病患者106例(帕金森病组),并选择同期体检中心健康体检者104例(对照组)。收集所有参加者年龄、性别、血尿酸、血清25-羟维生素D3等基线资料,并进行组间比较。结果帕金森病组血尿酸和25羟维生素D3水平明显低于对照组[(263.24±105.02)μmol/L vs(304.11±79.31)μmol/L,P=0.002;(27.14±6.45)μg/L vs(36.96±11.62)μg/L,P=0.001]。logistic回归分析显示,年龄(OR=1.024,95%CI:1.001~1.081,P=0.028)、血尿酸(OR=0.996,95%CI:0.992~1.000,P=0.001)、25-羟维生素D3(OR=0.890,95%CI:0.852~0.929,P=0.000)与帕金森病显著相关。结论帕金森病患者血尿酸、血清25-羟维生素D3水平显著降低,高水平的血尿酸、血清25-羟维生素D3很可能是帕金森病保护因素。

基线尿酸水平与缺血性脑卒中患者预后的相关性

目的探讨急性缺血性脑卒中患者基线尿酸水平与临床预后的相关性。方法收集240例急性缺血性脑卒中患者,并记录基线尿酸水平,根据基线尿酸水平将研究对象分为4组,比较发病14 d及发病90 d时的神经功能情况。结果随基线尿酸水平的增高,发病14 d时神经功能缺损情况较发病1 d时好转(P<0.05),4组间比较差异有统计学意义(P<0.05)。发病90 d时,基线尿酸水平越高,患者的转归良好率越增高(P<0.05),当尿酸高于289.55 mol/L,患者转归良好率明显增高,差异有统计学意义(P<0.05)。随尿酸水平的增高,患者发病90 d时的不良事件的发生率无明显变化,4组间比较差异无统计学意义(P>0.05)。结论基线尿酸水平越高,患者神经功能恢复及临床转归越好。且当尿酸高于289.55 mol/L,患者转归良好率明显增高。

血清尿酸水平变化在急性脑梗死患者尿激酶溶栓治疗中的临床意义

目的探讨急性脑梗死患者溶栓治疗前后血清尿酸水平变化及意义。方法选择急性脑梗死患者59例,均进行尿激酶治疗,根据治疗效果分为好转组36例和无好转组23例。检测2组溶栓前、溶栓第2、7天及发病3个月后血清尿酸水平。结果与无好转组比较,好转组溶栓第2天尿酸水平明显下降,差异有统计学意义(P<0.05).2组溶栓治疗前及溶栓第7天以及3个月后尿酸水平比较,差异无统计学意义(P>0.05)。结论尿酸有可能在缺血再灌注过程中发挥一定作用,存在溶栓后尿酸水平衰减。