With Fusarium oxysporum f.sp. phaseoli as the target bacterium,the bacterial strain with strong antagonistic activity was screened from crop rhizosphere soil using plate confrontation method and pot efficiency test. ...With Fusarium oxysporum f.sp. phaseoli as the target bacterium,the bacterial strain with strong antagonistic activity was screened from crop rhizosphere soil using plate confrontation method and pot efficiency test. This strain was identified according to morphological observation, physiological and biochemical characteristics and sequence analysis of 16S rRNA gene and gyrB gene. The results showed that LZF-5-3 strain was identified as Bacillus methylotrophicus and the control effect of LZF-5-3 against F.oxysporum in snap bean was 71.42% in pot test.展开更多
In this paper,we reviewed the classification,genetic diversity and planting and application of snap bean(Phaseolus vulgaris)germplasm resources in China at the present stage were,analyzed the bottleneck of snap bean r...In this paper,we reviewed the classification,genetic diversity and planting and application of snap bean(Phaseolus vulgaris)germplasm resources in China at the present stage were,analyzed the bottleneck of snap bean research in China,and prospected the development trend of snap bean germplasm research in the future.In the opportunity of the completion of the whole genome sequencing of kidney bean and the construction of high-density genetic map,it is necessary to strengthen the application of ISSR,SNP and other molecular methods and high-throughput proteomics to the study on the specific breeding of snap bean.展开更多
The effects of arbuscular mycorrhizal fungi（AMF） Glomus mosseae on the responses to elevated O3 in growth and nutrition of snap bean（Phaseolus vulgaris L.cv Guangzhouyuan） were investigated.Exposure was conducted ...The effects of arbuscular mycorrhizal fungi（AMF） Glomus mosseae on the responses to elevated O3 in growth and nutrition of snap bean（Phaseolus vulgaris L.cv Guangzhouyuan） were investigated.Exposure was conducted in growth chambers by using three O3 concentrations（20（CF）,80（CFO1） and 120 nL/L（CFO2）;8 hr/day for 75 days）.Results showed that elevated O3 slightly impacted overall mycorrhizal colonization,but significantly decreased the proportional frequency of hypha and increased the proportional frequency of spores and vesicles,suggesting that O3 had significant effects on mycorrhizal structure.Elevated O3 significantly decreased yield,dry mass and nutrient contents（N,P,K,Ca and Mg） in both non-mycorrhizal and mycorrhizal plants.However,significant interactive effects were found in most variables due to that the reduction by O3 in the mycorrhizal plants was less than that in the non-mycorrhizal plants.Additionally,AMF increased the concentrations of N,P,Ca,and Mg in shoot and root.It can be concluded that AMF alleviated detrimental effects of increasing O3 on host plant through improving plant nutrition and growth.展开更多
文摘With Fusarium oxysporum f.sp. phaseoli as the target bacterium,the bacterial strain with strong antagonistic activity was screened from crop rhizosphere soil using plate confrontation method and pot efficiency test. This strain was identified according to morphological observation, physiological and biochemical characteristics and sequence analysis of 16S rRNA gene and gyrB gene. The results showed that LZF-5-3 strain was identified as Bacillus methylotrophicus and the control effect of LZF-5-3 against F.oxysporum in snap bean was 71.42% in pot test.
基金Supported by Jilin Provincial Science and Technology Development Program:International Science and Technology Cooperation(20200801075GH)Jilin Provincial Agricultural Science and Technology Innovation Project(CXGC2021ZY101)。
文摘In this paper,we reviewed the classification,genetic diversity and planting and application of snap bean(Phaseolus vulgaris)germplasm resources in China at the present stage were,analyzed the bottleneck of snap bean research in China,and prospected the development trend of snap bean germplasm research in the future.In the opportunity of the completion of the whole genome sequencing of kidney bean and the construction of high-density genetic map,it is necessary to strengthen the application of ISSR,SNP and other molecular methods and high-throughput proteomics to the study on the specific breeding of snap bean.
基金supported by the National Natural Science Foundation of China(No.40701180,30670387)the Chinese Universities Scientific Fund(No.ZZ1106, ZD0904)the Project of Beijing Key Discipline and State Key Laboratory of Urban and Regional Ecology
文摘The effects of arbuscular mycorrhizal fungi（AMF） Glomus mosseae on the responses to elevated O3 in growth and nutrition of snap bean（Phaseolus vulgaris L.cv Guangzhouyuan） were investigated.Exposure was conducted in growth chambers by using three O3 concentrations（20（CF）,80（CFO1） and 120 nL/L（CFO2）;8 hr/day for 75 days）.Results showed that elevated O3 slightly impacted overall mycorrhizal colonization,but significantly decreased the proportional frequency of hypha and increased the proportional frequency of spores and vesicles,suggesting that O3 had significant effects on mycorrhizal structure.Elevated O3 significantly decreased yield,dry mass and nutrient contents（N,P,K,Ca and Mg） in both non-mycorrhizal and mycorrhizal plants.However,significant interactive effects were found in most variables due to that the reduction by O3 in the mycorrhizal plants was less than that in the non-mycorrhizal plants.Additionally,AMF increased the concentrations of N,P,Ca,and Mg in shoot and root.It can be concluded that AMF alleviated detrimental effects of increasing O3 on host plant through improving plant nutrition and growth.
