A feeding trial was conducted in a recirculating water system to investigatethe effects of dietary protein levels on growth, feed utilization, hepatosomatic index and liverlipid deposition of juvenile red snapper, Lut...A feeding trial was conducted in a recirculating water system to investigatethe effects of dietary protein levels on growth, feed utilization, hepatosomatic index and liverlipid deposition of juvenile red snapper, Lutjanus argentimaculatus (average initial wet weight 8.0± 0.39 g and total length 3.14 ± 0.3 cm). In the experiment, six fishmeal-based diets wereformulated to contain various protein levels (20% to 45% in 5% increments), with dietary energyranging from 2210.7kJ100g to 2250.2 kJ 100g dry matter. The protein to energy ratios of diets rangedfrom 8.58 mg protein kJ^(-1) to 20.03 mg protein kJ^(-1). Diets were fed for 90 d to triplicategroups of fish stocked in 0.128 m^3 seawater tanks, 25 individuals each. The daily ration of 2% wetbody weight was offered to the fish thrice a day. The fish at the end of the study had more thanten-fold (77.0 g) increase in weight compared to the initial (8.0 g). Fish fed diets of 40% and 45%protein produced significantly (P【0.05) higher weight gain of 77.2 g and 76.5 g, and specific growthrate (SGR) of 2.65% and 2.62% than those of 67.0 g and 68.3 g, and 2.49% and 2.51% of the otherdiets. The broken-line regression of SGR against dietary protein level yielded an optimum dietaryprotein requirement of 42.6% (Y = - 1.6295 + 0.1114 X^2, P【0.05). Survival remained 100% amonggroups. Feed conversion ratio decreased from 0.45 for fish fed 20% dietary protein to 0.35 for fishfed 45% dietary protein. Nitrogen intake increased with an increase in dietary protein, which inturn resulted in an increase in nitrogen gain of fish whole body. Fish fed 40% and 45% protein dietsshowed higher (P【0.05) nitrogen gain (0.27g and 0.26g) than those (0.23g and 025g) fed all otherdiets. Gross energy intake (GEI) in fish fed 45% protein was lower (600.67kJ) than that (607.97 kJ)of 40% protein diet, though the differences were not statistically significant (P】0.05); GEI rangingfrom 677.31 kJ to 663.20 kJ at remaining four diets (20% to 35% protein) did not appear to differsignificantly (P】0.05). The highest energy gain of 518.33 kJ was obtained with fish fed 40% protein,resulting in the highest energy retention efficiency of 85.26%. The hepatosomatic index of fish feddiets of 20%, 25%, 30% and 35% protein were significantly (P【0.05) higher (2.09% to 2.57%) thanthose (1.44% and 1.41%) of fish fed diets containing 40% and 45% protein. Liver lipid contentsdecreased from 8.72% to 7.0% in fish fed dietary protein of 20% to 45% in 5% increments. Resultssuggest that the diet containing 40% to 42.6% protein with a P/E ratio of 17.6 mg protein kJ^(-1) isrequired for good growth of L. argentimaculatus weighing between 8.0 g and 85.2 g under the cultureconditions of the present study.展开更多
A feeding trial was conducted for 75 d to evaluate the nutritive value of a mixture of animal by-products （MAB） as a possible protein source in diets for juvenile mangrove red snapper, Lutjanus argentimaculatus （me...A feeding trial was conducted for 75 d to evaluate the nutritive value of a mixture of animal by-products （MAB） as a possible protein source in diets for juvenile mangrove red snapper, Lutjanus argentimaculatus （mean initial body weight, 30 g）. Fish were fed one of five isonitrogenous diets （40% crude protein） replacing 0, 25% （MAB25）, 50% （MAB50）, 75% （MAB75） and 100% （MAB100） of fish meal protein with similar percentages of MAB. The MAB consisted of 25% cow liver meal, 20% leather meal, 20% meat and bone meal, 15% blood meal, 10% APC （poultry feather meal）, 8% poultry manure dried, 1.5% choline and 0.5% chromic oxide. After 75 d of feeding, fish fed with diets MAB50, MAB75 and MABI00 exhibited significantly lower growth performance than that of fish fed with control and MAB25 diets. The optimum level of MAB was estimated to be 23%. Replacement of fish meal by MAB23% showed the following performance： maximum weight gain, 510%; SGR, 2.39% and FCE, 2.83%. The MAB substitution up to 75% of fish meal protein in diets did not show differences in apparent protein digestibility （83.6% for MAB25, 79.2% for MAB50, 78.7% for MAB75） compared with control （83.4%）, whereas in MABI00 group digestibility （65.3%） was significantly lower than in other groups. The apparent phosphorus absorption of test diet groups was significantly higher （37.1% for MAB25, 28.5% for MABS0, 55.6% for MAB75 and 54.5% for MABI00） than that of control （1 1.2%）. The levels of protein and ash in the whole body, carcass and viscera increased as MAB substitution in diets increased, whereas lipids and moisture remained consistent among all treatment groups. These results showed that approximately 23% of fish meal protein could be replaced by a mixture of animal by-products for juvenile snapper growing from 30 g to 167 g in 75 d without compromising growth performance and feed efficiency.展开更多
Roes of yellowfin tuna （Thunnus albacares） and red snapper （Lutjanus champecanus） are considered as abundant and underutilized by-product in Indonesia. The production of roe protein concentrate （RPC） is expected...Roes of yellowfin tuna （Thunnus albacares） and red snapper （Lutjanus champecanus） are considered as abundant and underutilized by-product in Indonesia. The production of roe protein concentrate （RPC） is expected to increase the economic value and potency of their usage. Tuna and red snapper RPC was defatted using etano195% with one, two, three and four times of repetition. Both RPC made with four times repetition of defatting had the highest protein content （79.90% and 80.72%）, showed high emulsion activity （97.46% and 99.62%） and emulsion stability （97.10% and 99.48%）, and decreased the interfacial tension of 51% and 55.9%, respectively. Mayonnaise was made with 0, 25, 50, 75 and 100% substitution level of each RPC with four times repetition ofdefatting. The physical, chemical and organoleptic properties of mayonnaise were studied. The best mayonnaise formulation was obtained from 50% substitution level of tuna and red snapper RPC. Mayonnaise with tuna and red snapper RPC had good viscosity （5,920 cPs and 5,845 cPs）, high emulsion stability （90.5% and 91.73%） and small fat globule size （±2.5 lam and ± 2.25 lam）, respectively. These mayonnaises also showed high score of spreadability and low intense of fishy odor. However they still had quite strong of fishy flavor based on scoring test.展开更多
In this study we aimed to analyze the effects of water temperature and diet on the length-weight rela- tionship and condition of juvenile Malabar blood snapper Lutjanus malabaricus over a 30-d experimental period. The...In this study we aimed to analyze the effects of water temperature and diet on the length-weight rela- tionship and condition of juvenile Malabar blood snapper Lutjanus malabaricus over a 30-d experimental period. The experiment was conducted in the laboratory using a flow-through-sea-water system. The fish were subjected to four different temperatures （22, 26, 30, and 34 ℃） and two diets （commercial pellet and natural shrimp）. Fish were fed twice daily. L. malabancus exhibited negative allometric growth （b〈3） at the beginning of the experiment （Day 0） at all temperatures and both diets except for 22 ℃ fed with shrimp, which showed isometric growth （b=3）. Conversely, at the end of the experiment （Day 30） fish showed isometric growth （b=3） at 30 ℃ fed with the pellet diet, indicating that the shape of the fish did not change with increasing weight and length, and a positive allometric growth （b〉3） at 30 ℃ fed with shrimp diet, which indicated that fish weight increases faster than their length. The rest of the temperatures represented negative allometric growth （b〈3） on both diet, meaning that fish became lighter with increasing size. The condition factors in the initial and final measurements were greater than 1, indicating the state of health of the fish, except for those fed on a pellet diet at 34 ℃. However, the best condition was obtained at 30 ℃ on both diets. Nev- ertheless, diets did not have a significant effect on growth and condition of juvenile L. malabaricus. The data obtained from this study suggested culturing L. malabaricus at 30 ℃ and feeding on the pellet or shrimp diet, which will optimize the overall production and condition of this commercially important fish species.展开更多
The genetic population structure of red snapper Lutjanus malabaricus and orange-spotted grouper Epinepheluscoiodes in Brunei and Sabah was investigated using allozyme electrophoresis. Samples were collected from three...The genetic population structure of red snapper Lutjanus malabaricus and orange-spotted grouper Epinepheluscoiodes in Brunei and Sabah was investigated using allozyme electrophoresis. Samples were collected from threesites in Brunei for E. coiodes and from three sites in Brunei and Sabah for L. malabaricus. A total of 22 loci and 16loci were scored, respectively. The index of fixation (FST) for the E. coiodes population was 0.176 but, in general, itlacked within-population structuring. The FST was particularly high between Brunei Bay/Jerudong and Brunei Bay/Kuala Belait, suggesting genetic subdivision on a small spatial scale. Isolation of Brunei Bay from the South ChinaSea may constrain the movement of adult fishes and larval dispersal, thereby reducing homogeneity amongsubpopulations. All variable loci for E. coiodes were in Hardy–Weinberg equilibrium except for MDH* and GTDHP*(P < 0.01), in which two subpopulations showed an excess of heterozygotes (P < 0.01). The study on the L.malabaricus population showed a heterozygote deficit of approximately 60% in variable loci (FIS, genetic variationwithin population = 0.45;P < 0.05);however, the mean observed heterozygosity for the population far exceeded L.malabaricus populations in Australia and Indonesia. A FST value of 0.076 revealed moderate genetic differentiationamong subpopulations of L. malabaricus. The genotypes were likely to be drawn from the same distribution inJerudong and Kuala Belait. This study infers that sustainable management of snapper and grouper resources inBrunei waters must take into account the presence of a single stock and two stocks, respectively.展开更多
通过显微直接观察法对红鳍笛鲷胚胎发生的形态学进行了研究,以期为红鳍笛鲷人工繁殖、育苗提供参考资料。红鳍笛鲷胚胎的形态发生过程可分为受精卵、卵裂、囊胚、原肠胚、神经胚、器官形成等6个阶段,24个时期。红鳍笛鲷受精卵为浮性卵,...通过显微直接观察法对红鳍笛鲷胚胎发生的形态学进行了研究,以期为红鳍笛鲷人工繁殖、育苗提供参考资料。红鳍笛鲷胚胎的形态发生过程可分为受精卵、卵裂、囊胚、原肠胚、神经胚、器官形成等6个阶段,24个时期。红鳍笛鲷受精卵为浮性卵,透明而呈圆球形,内有一大油球,在水温28℃、海水盐度32.2的条件下,受精后15 min原生质隆起形成胚盘;受精后30 min进行第1次卵裂,为盘状卵裂;受精后3 h,胚胎进入高囊胚期;受精后6 h 27 min,胚胎发育至原肠期;受精后9 h 36 min形成神经板,继而神经板前端分化出前脑泡、中脑泡和后脑泡;脑泡分化后,视囊、听囊、晶状体等相继形成;受精后15 h 32 min尾芽形成;受精后约20 h,仔鱼出膜。其发育过程与黄鲷、金头鲷、点带石斑等其他海水鱼相似。展开更多
文摘A feeding trial was conducted in a recirculating water system to investigatethe effects of dietary protein levels on growth, feed utilization, hepatosomatic index and liverlipid deposition of juvenile red snapper, Lutjanus argentimaculatus (average initial wet weight 8.0± 0.39 g and total length 3.14 ± 0.3 cm). In the experiment, six fishmeal-based diets wereformulated to contain various protein levels (20% to 45% in 5% increments), with dietary energyranging from 2210.7kJ100g to 2250.2 kJ 100g dry matter. The protein to energy ratios of diets rangedfrom 8.58 mg protein kJ^(-1) to 20.03 mg protein kJ^(-1). Diets were fed for 90 d to triplicategroups of fish stocked in 0.128 m^3 seawater tanks, 25 individuals each. The daily ration of 2% wetbody weight was offered to the fish thrice a day. The fish at the end of the study had more thanten-fold (77.0 g) increase in weight compared to the initial (8.0 g). Fish fed diets of 40% and 45%protein produced significantly (P【0.05) higher weight gain of 77.2 g and 76.5 g, and specific growthrate (SGR) of 2.65% and 2.62% than those of 67.0 g and 68.3 g, and 2.49% and 2.51% of the otherdiets. The broken-line regression of SGR against dietary protein level yielded an optimum dietaryprotein requirement of 42.6% (Y = - 1.6295 + 0.1114 X^2, P【0.05). Survival remained 100% amonggroups. Feed conversion ratio decreased from 0.45 for fish fed 20% dietary protein to 0.35 for fishfed 45% dietary protein. Nitrogen intake increased with an increase in dietary protein, which inturn resulted in an increase in nitrogen gain of fish whole body. Fish fed 40% and 45% protein dietsshowed higher (P【0.05) nitrogen gain (0.27g and 0.26g) than those (0.23g and 025g) fed all otherdiets. Gross energy intake (GEI) in fish fed 45% protein was lower (600.67kJ) than that (607.97 kJ)of 40% protein diet, though the differences were not statistically significant (P】0.05); GEI rangingfrom 677.31 kJ to 663.20 kJ at remaining four diets (20% to 35% protein) did not appear to differsignificantly (P】0.05). The highest energy gain of 518.33 kJ was obtained with fish fed 40% protein,resulting in the highest energy retention efficiency of 85.26%. The hepatosomatic index of fish feddiets of 20%, 25%, 30% and 35% protein were significantly (P【0.05) higher (2.09% to 2.57%) thanthose (1.44% and 1.41%) of fish fed diets containing 40% and 45% protein. Liver lipid contentsdecreased from 8.72% to 7.0% in fish fed dietary protein of 20% to 45% in 5% increments. Resultssuggest that the diet containing 40% to 42.6% protein with a P/E ratio of 17.6 mg protein kJ^(-1) isrequired for good growth of L. argentimaculatus weighing between 8.0 g and 85.2 g under the cultureconditions of the present study.
文摘A feeding trial was conducted for 75 d to evaluate the nutritive value of a mixture of animal by-products （MAB） as a possible protein source in diets for juvenile mangrove red snapper, Lutjanus argentimaculatus （mean initial body weight, 30 g）. Fish were fed one of five isonitrogenous diets （40% crude protein） replacing 0, 25% （MAB25）, 50% （MAB50）, 75% （MAB75） and 100% （MAB100） of fish meal protein with similar percentages of MAB. The MAB consisted of 25% cow liver meal, 20% leather meal, 20% meat and bone meal, 15% blood meal, 10% APC （poultry feather meal）, 8% poultry manure dried, 1.5% choline and 0.5% chromic oxide. After 75 d of feeding, fish fed with diets MAB50, MAB75 and MABI00 exhibited significantly lower growth performance than that of fish fed with control and MAB25 diets. The optimum level of MAB was estimated to be 23%. Replacement of fish meal by MAB23% showed the following performance： maximum weight gain, 510%; SGR, 2.39% and FCE, 2.83%. The MAB substitution up to 75% of fish meal protein in diets did not show differences in apparent protein digestibility （83.6% for MAB25, 79.2% for MAB50, 78.7% for MAB75） compared with control （83.4%）, whereas in MABI00 group digestibility （65.3%） was significantly lower than in other groups. The apparent phosphorus absorption of test diet groups was significantly higher （37.1% for MAB25, 28.5% for MABS0, 55.6% for MAB75 and 54.5% for MABI00） than that of control （1 1.2%）. The levels of protein and ash in the whole body, carcass and viscera increased as MAB substitution in diets increased, whereas lipids and moisture remained consistent among all treatment groups. These results showed that approximately 23% of fish meal protein could be replaced by a mixture of animal by-products for juvenile snapper growing from 30 g to 167 g in 75 d without compromising growth performance and feed efficiency.
文摘Roes of yellowfin tuna （Thunnus albacares） and red snapper （Lutjanus champecanus） are considered as abundant and underutilized by-product in Indonesia. The production of roe protein concentrate （RPC） is expected to increase the economic value and potency of their usage. Tuna and red snapper RPC was defatted using etano195% with one, two, three and four times of repetition. Both RPC made with four times repetition of defatting had the highest protein content （79.90% and 80.72%）, showed high emulsion activity （97.46% and 99.62%） and emulsion stability （97.10% and 99.48%）, and decreased the interfacial tension of 51% and 55.9%, respectively. Mayonnaise was made with 0, 25, 50, 75 and 100% substitution level of each RPC with four times repetition ofdefatting. The physical, chemical and organoleptic properties of mayonnaise were studied. The best mayonnaise formulation was obtained from 50% substitution level of tuna and red snapper RPC. Mayonnaise with tuna and red snapper RPC had good viscosity （5,920 cPs and 5,845 cPs）, high emulsion stability （90.5% and 91.73%） and small fat globule size （±2.5 lam and ± 2.25 lam）, respectively. These mayonnaises also showed high score of spreadability and low intense of fishy odor. However they still had quite strong of fishy flavor based on scoring test.
基金supported by the Ministry of Science Technology and Innovation Malaysia(MOSTI)(No.04-01-02-SF1208)
文摘In this study we aimed to analyze the effects of water temperature and diet on the length-weight rela- tionship and condition of juvenile Malabar blood snapper Lutjanus malabaricus over a 30-d experimental period. The experiment was conducted in the laboratory using a flow-through-sea-water system. The fish were subjected to four different temperatures （22, 26, 30, and 34 ℃） and two diets （commercial pellet and natural shrimp）. Fish were fed twice daily. L. malabancus exhibited negative allometric growth （b〈3） at the beginning of the experiment （Day 0） at all temperatures and both diets except for 22 ℃ fed with shrimp, which showed isometric growth （b=3）. Conversely, at the end of the experiment （Day 30） fish showed isometric growth （b=3） at 30 ℃ fed with the pellet diet, indicating that the shape of the fish did not change with increasing weight and length, and a positive allometric growth （b〉3） at 30 ℃ fed with shrimp diet, which indicated that fish weight increases faster than their length. The rest of the temperatures represented negative allometric growth （b〈3） on both diet, meaning that fish became lighter with increasing size. The condition factors in the initial and final measurements were greater than 1, indicating the state of health of the fish, except for those fed on a pellet diet at 34 ℃. However, the best condition was obtained at 30 ℃ on both diets. Nev- ertheless, diets did not have a significant effect on growth and condition of juvenile L. malabaricus. The data obtained from this study suggested culturing L. malabaricus at 30 ℃ and feeding on the pellet or shrimp diet, which will optimize the overall production and condition of this commercially important fish species.
基金the Universiti Brunei Darussalam through research grant no.PNC2/2/RG/1(18).
文摘The genetic population structure of red snapper Lutjanus malabaricus and orange-spotted grouper Epinepheluscoiodes in Brunei and Sabah was investigated using allozyme electrophoresis. Samples were collected from threesites in Brunei for E. coiodes and from three sites in Brunei and Sabah for L. malabaricus. A total of 22 loci and 16loci were scored, respectively. The index of fixation (FST) for the E. coiodes population was 0.176 but, in general, itlacked within-population structuring. The FST was particularly high between Brunei Bay/Jerudong and Brunei Bay/Kuala Belait, suggesting genetic subdivision on a small spatial scale. Isolation of Brunei Bay from the South ChinaSea may constrain the movement of adult fishes and larval dispersal, thereby reducing homogeneity amongsubpopulations. All variable loci for E. coiodes were in Hardy–Weinberg equilibrium except for MDH* and GTDHP*(P < 0.01), in which two subpopulations showed an excess of heterozygotes (P < 0.01). The study on the L.malabaricus population showed a heterozygote deficit of approximately 60% in variable loci (FIS, genetic variationwithin population = 0.45;P < 0.05);however, the mean observed heterozygosity for the population far exceeded L.malabaricus populations in Australia and Indonesia. A FST value of 0.076 revealed moderate genetic differentiationamong subpopulations of L. malabaricus. The genotypes were likely to be drawn from the same distribution inJerudong and Kuala Belait. This study infers that sustainable management of snapper and grouper resources inBrunei waters must take into account the presence of a single stock and two stocks, respectively.
文摘通过显微直接观察法对红鳍笛鲷胚胎发生的形态学进行了研究,以期为红鳍笛鲷人工繁殖、育苗提供参考资料。红鳍笛鲷胚胎的形态发生过程可分为受精卵、卵裂、囊胚、原肠胚、神经胚、器官形成等6个阶段,24个时期。红鳍笛鲷受精卵为浮性卵,透明而呈圆球形,内有一大油球,在水温28℃、海水盐度32.2的条件下,受精后15 min原生质隆起形成胚盘;受精后30 min进行第1次卵裂,为盘状卵裂;受精后3 h,胚胎进入高囊胚期;受精后6 h 27 min,胚胎发育至原肠期;受精后9 h 36 min形成神经板,继而神经板前端分化出前脑泡、中脑泡和后脑泡;脑泡分化后,视囊、听囊、晶状体等相继形成;受精后15 h 32 min尾芽形成;受精后约20 h,仔鱼出膜。其发育过程与黄鲷、金头鲷、点带石斑等其他海水鱼相似。
