目的利用PANDORA-seq鉴定年轻和老年小鼠心房组织中的sncRNA表达差异,观察姜黄素干预对老年小鼠差异sncRNA和靶基因的影响。方法年轻组(5个月龄)和老年组(18个月龄)小鼠心房组织进行PANDORA-seq,SPORTS1.1注释sncRNA的表达谱,R包DESeq2...目的利用PANDORA-seq鉴定年轻和老年小鼠心房组织中的sncRNA表达差异,观察姜黄素干预对老年小鼠差异sncRNA和靶基因的影响。方法年轻组(5个月龄)和老年组(18个月龄)小鼠心房组织进行PANDORA-seq,SPORTS1.1注释sncRNA的表达谱,R包DESeq2展示差异sncRNA,对差异miRNA进行靶基因预测和构建miRNA-mRNA网络,实时定量聚合酶链反应(quantitative real time polymerase chain reaction,qRT-PCR)鉴定靶分子。姜黄素干预后,在体电生理标测和Western-blot观察老年小鼠心房颤动可诱发率,心房衰老程度及衰老和纤维化相关蛋白的表达。结果从年轻组和老年组小鼠心房组织中鉴定出大量sncRNA,包括miRNA、tsRNA、rsRNA和piRNA,老年组sncRNA表达丰度比年轻组明显增多(P=0.048),rsRNA和tsRNA的起源位点也存在差异。两组小鼠间4种sncRNA存在大量序列差异,而其种类差异只存在于miRNA中,且老年组小鼠miR-298(P=0.005)和miR-301b(P=0.004)明显降低。姜黄素干预可使老年小鼠心房组织中miR-298(P=0.000)和miR-301b(P=0.000)表达上调,降低衰老和纤维化蛋白的表达和改善心房颤动易感性。结论sncRNA参与小鼠心房衰老过程,姜黄素可靶向miR-298和miR-301b参与心房衰老相关纤维化过程,为老年心房颤动的防治提供新思路。展开更多
Objective:To give a brief overview of the field of epigenetics and the potential predictive power that small non-coding RNA(sncRNA)may hold in relation to improving the treatment and diagnosis of male infertility.Meth...Objective:To give a brief overview of the field of epigenetics and the potential predictive power that small non-coding RNA(sncRNA)may hold in relation to improving the treatment and diagnosis of male infertility.Methods:PRISMA-ScR was used as the scoping review guideline for this investigation.All article data here have been accessed from MEDLINE–PubMed,Science Direct,EBSCO,Scopus,Sage Journals,and Google Scholar.The terms"small non coding RNA,male,infertility,miRNA,sperm"were used in the search between 2015 and 2023.Results:The study comprised 35 publications in total.Several sncRNAs,miR-155,miR-16,miR-196,miR-525-3p,miR-891 were found to be effective in regulating the mechanism of spermatozoa processing in the infertility of men.sncRNA can be used as a biomarker of male infertility.Conclusions:sncRNAs can act as biomarkers for the diagnosis of reproductive diseases.Actually,by recognizing sncRNAs and their mechanisms,a new way to treat infertile men would be paved.The functional annotation of sncRNAs in spermatogenesis is still in its infancy but has enormous potential.This is despite the fact that many potential sncRNAs have been found to date with the use of cutting-edge technology and publicly accessible sncRNA annotation tools.展开更多
文摘目的利用PANDORA-seq鉴定年轻和老年小鼠心房组织中的sncRNA表达差异,观察姜黄素干预对老年小鼠差异sncRNA和靶基因的影响。方法年轻组(5个月龄)和老年组(18个月龄)小鼠心房组织进行PANDORA-seq,SPORTS1.1注释sncRNA的表达谱,R包DESeq2展示差异sncRNA,对差异miRNA进行靶基因预测和构建miRNA-mRNA网络,实时定量聚合酶链反应(quantitative real time polymerase chain reaction,qRT-PCR)鉴定靶分子。姜黄素干预后,在体电生理标测和Western-blot观察老年小鼠心房颤动可诱发率,心房衰老程度及衰老和纤维化相关蛋白的表达。结果从年轻组和老年组小鼠心房组织中鉴定出大量sncRNA,包括miRNA、tsRNA、rsRNA和piRNA,老年组sncRNA表达丰度比年轻组明显增多(P=0.048),rsRNA和tsRNA的起源位点也存在差异。两组小鼠间4种sncRNA存在大量序列差异,而其种类差异只存在于miRNA中,且老年组小鼠miR-298(P=0.005)和miR-301b(P=0.004)明显降低。姜黄素干预可使老年小鼠心房组织中miR-298(P=0.000)和miR-301b(P=0.000)表达上调,降低衰老和纤维化蛋白的表达和改善心房颤动易感性。结论sncRNA参与小鼠心房衰老过程,姜黄素可靶向miR-298和miR-301b参与心房衰老相关纤维化过程,为老年心房颤动的防治提供新思路。
文摘Objective:To give a brief overview of the field of epigenetics and the potential predictive power that small non-coding RNA(sncRNA)may hold in relation to improving the treatment and diagnosis of male infertility.Methods:PRISMA-ScR was used as the scoping review guideline for this investigation.All article data here have been accessed from MEDLINE–PubMed,Science Direct,EBSCO,Scopus,Sage Journals,and Google Scholar.The terms"small non coding RNA,male,infertility,miRNA,sperm"were used in the search between 2015 and 2023.Results:The study comprised 35 publications in total.Several sncRNAs,miR-155,miR-16,miR-196,miR-525-3p,miR-891 were found to be effective in regulating the mechanism of spermatozoa processing in the infertility of men.sncRNA can be used as a biomarker of male infertility.Conclusions:sncRNAs can act as biomarkers for the diagnosis of reproductive diseases.Actually,by recognizing sncRNAs and their mechanisms,a new way to treat infertile men would be paved.The functional annotation of sncRNAs in spermatogenesis is still in its infancy but has enormous potential.This is despite the fact that many potential sncRNAs have been found to date with the use of cutting-edge technology and publicly accessible sncRNA annotation tools.
文摘哺乳动物胚胎发育受遗传和表观遗传的共同调控。精子作为重要的雄性生殖细胞,通过受精过程,将这些信息传递给卵子,进而影响子代的发育。精子中携带有丰富的表观遗传信息,其中小非编码RNAs(small noncoding RNAs,sncRNAs)在精子发育不同阶段发挥重要的作用,包括调控基因表达、介导蛋白质翻译,以及参与精子的表观遗传信息传递等。环境暴露包括饮食变化、毒性物质暴露和心理压力等。现有的研究表明,环境因素不仅影响机体健康,还可能导致生殖系统配子(精子与/或卵子)表观遗传信息的改变。越来越多的证据表明,亲本在环境暴露后发生的获得性性状变化,可通过配子的表观遗传信息传递给后代,即产生跨代遗传。本综述主要讨论因环境因素引起的获得性性状,可通过精子sncRNAs变化,产生跨代遗传,并影响胚胎发育及子代健康。本综述的讨论主要集中在tRNA来源的小RNAs(transfer RNA-derived small RNAs,tsRNAs)、微RNA(microRNAs,miRNAs)和PIWI相互作用RNAs(PIWI-interacting RNAs,piRNAs),并涉及到最近在精子中发现有大量表达的rRNA来源的小RNAs(risbosome-RNA derived small RNAs,rsRNAs)。此外,本文还进一步探讨了环境因素影响精子sncRNAs表达变化的可能机制。通过对上述内容的综述,将更好地理解精子sncRNAs在跨代遗传中的作用,促进表观遗传学领域的新研究,加深对基本生命过程的理解。
