Osteoclasts are bone-specific multinucleated cells generated by the differentiation of monocyte/macrophage lineage precursors. Regulation of osteoclast differentiation is considered an effective therapeutic approach t...Osteoclasts are bone-specific multinucleated cells generated by the differentiation of monocyte/macrophage lineage precursors. Regulation of osteoclast differentiation is considered an effective therapeutic approach to the treatment of bone-lytic diseases. Periodontitis is an inflammatory disease characterized by extensive bone resorption. In this study, we investigated the effects of sodium fluoride （NaF） on osteoclastogenesis induced by Porphyromonas gingivalis, an important colonizer of the oral cavity that has been implicated in periodontitis. NaF strongly inhibited the P. gingivalis-induced alveolar bone loss. That effect was accompanied by decreased levels of cathepsin K, interleukin （IL）-1β, matrix metalloproteinase 9 （MMP9）, and tartrate-resistant acid phosphatase, which were up-regulated during P. gingivalis-induced osteoclastogenesis. Consistent with the in vivo anti-osteoclastogenic effect, NaF inhibited osteoclast formation caused by the differentiation factor RANKL （receptor activator of nuclear factor KB ligand） and macrophage colony-stimulating factor （M-CSF）. The RANKL-stimulated induction of the transcription factor nuclear factor of activated T cells （NFAT） cl was also abrogated by NaF. Taken together, our data demonstrate that NaF inhibits RANKL-induced osteoclastogenesis by reducing the induction of NFATcl, ultimately leading to the suppressed expression of cathepsin K and MMP9. The in vivo effect of NaF on the inhibition of P. gingivalis-induced osteoclastogenesis strengthens the potential usefulness of NaF for treating periodontal diseases.展开更多
Objective To investigate the toxic effect of sodium fluoride（NaF） on the nematode Caenorhabditis elegans（C.elegans）.Methods Adult C.elegans were exposed to different concentrations of NaF（0.038 mmol/L,0.38 mmol/L...Objective To investigate the toxic effect of sodium fluoride（NaF） on the nematode Caenorhabditis elegans（C.elegans）.Methods Adult C.elegans were exposed to different concentrations of NaF（0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L） for 24 h.To assess the physiological effects of NaF,the brood size,life span,head thrashes,and body bend frequency were examined.Reactive oxygen species（ROS） and cell apoptosis were detected as parameters of biochemical response.The gene expressions were determined by real‐time polymerase chain reaction（PCR） to assess the molecular‐level response.Results At the physiological level,the brood size of C.elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 6%,26%,and 28% respectively in comparison with the control group.The maximum life spans of C.elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 3 days and 5 days,respectively.Head thrashes and body bend frequency both decreased with increasing concentrations of NaF.At the biochemical level,the production of ROS and the incidence of cell apoptosis increased with increasing concentrations of NaF（P0.05）.At the molecular level,different concentrations of NaF exposure raised the expression of stress‐related genes,such as hsp16.1,sod‐3,ctl‐2,dhs‐28,gst‐1,and cep‐1.Conclusion NaF exposure could induce multiple biological toxicities to C.elegans in a concentration‐dependent manner.These toxicities may be relevant to the oxidative stress induced by increased ROS production and accumulation in C.elegans.展开更多
Sodium fluoride and high specific area silica were synthesized by using sodium hexafluorosilicate(Na2Si F6) and sodium carbonate decahydrate(Na2CO3·10H2O). The influencing factors of react temperature, contact ti...Sodium fluoride and high specific area silica were synthesized by using sodium hexafluorosilicate(Na2Si F6) and sodium carbonate decahydrate(Na2CO3·10H2O). The influencing factors of react temperature, contact time, sodium dodecyl sulfate(SDS) and molar ratio of Na2 Si F6 to Na2CO3·10H2O were investigated. The optimum process involves the reaction of 0.075 mol Na2 Si F6 and 150 m L, 0.225 mol Na2CO3·10H2O(molar ratio of 1:3) at 85 °C for 90 min, and 2.0×10-3 mol sodium dodecyl sulfate(SDS) as additive. The results show that the purities of Si O2 and Na F at extraction yields of 96.5% and 98.0% are 91.0% and 98.6%, respectively. The obtained Si O2 were characterized by X-ray diffraction(XRD), scanning electron microscope(SEM), Fourier transform infrared ray(FTIR), differential scanning calorimetry and thermogravimetric analysis(DSC-TGA), N2 absorption/desorption(BET) and laser particle size analyzer. The result demonstrates that Si O2 particles have a high BET surface area of 103 m2/g, and a mean grain size of 985 nm.展开更多
A method is described for the direct determination of iron in high purity sodium fluoride using graphite furnace atomic absorption spectrometry. Interferences caused by the matrix are investigated. It is shown that th...A method is described for the direct determination of iron in high purity sodium fluoride using graphite furnace atomic absorption spectrometry. Interferences caused by the matrix are investigated. It is shown that the ashing temperature can be increased to 1 400°C and matrix interferences eliminated, the sensitivity of iron increased in 1. 27 fold by the addition of nickel nitrate. The method is applied to the determination of iron in sodium fluoride and satisfactory results are obtained.展开更多
Objective:To investigate the effect of Camellia(C.)sinensis in mitigating oxidative damage and reproductive toxicity in testis induced by sodium fluoride in a rat model.Methods:Twenty-four adult male Wister rats were ...Objective:To investigate the effect of Camellia(C.)sinensis in mitigating oxidative damage and reproductive toxicity in testis induced by sodium fluoride in a rat model.Methods:Twenty-four adult male Wister rats were divided into 4 groups,with 6 rats in each group.Group 1 orally received distilled water(1 mL/100 g body weight)daily and served as the control group,while group 2 received drinking water with 100 ppm sodium fluoride per day for 21 consecutive days,group 3 was administered with only C.sinensis extract by gavage at a dose of 100 mg/kg body weight and group 4 received drinking water with 100 ppm sodium fluoride and 100 mg/kg body weight C.sinensis leaf extract per day for 21 consecutive days.At the end of the treatment,the rats were sacrificed under light ether anesthesia.The gonado-somatic index,sperm count and motility,serum level of luteinizing hormone and testosterone were assayed.Lipid peroxidation[malondialdehyde(MDA)level],nitric oxide(NO)production,and activities of antioxidant enzymes-superoxide dismutase(SOD),catalase,and reduced glutathione level(GSH)were also analysed.Results:Sodium fluoride treatment significantly decreased gonado-somatic index,sperm count and motility as well as the serum level of luteinizing hormone and testosterone(P<0.05).The histological examination of testes revealed atrophy and degenerative changes in several seminiferous tubules,along with enhanced interstitial space and a reduced number of Leydig cells.There was a highly significant increase in NO and MDA production(P<0.05),while SOD,catalase activities and GSH level decreased significantly(P<0.05).However,C.sinensis significantly restored testicular weight,sperm parameter,hormonal level(P<0.05),and also reversed MDA and NO generation and antioxidant enzymes activities in the testicular tissue(P<0.05).Conclusions:C.sinensis may have an ameliorative role against sodium fluoride-induced oxidative damage in the testis probably because of its antioxidant property.展开更多
The liver is a primary site for xenobiotics detoxification, and its metabolism is readily altered by toxicity. The kidney is a common target for toxic xenobiotics due to its capacity to extract and concentrate toxic s...The liver is a primary site for xenobiotics detoxification, and its metabolism is readily altered by toxicity. The kidney is a common target for toxic xenobiotics due to its capacity to extract and concentrate toxic substances by highly specialized cells. So, they are the target organs of sodium fluoride toxicity. The aim of this review is to highlight on hepatorenal oxidative stress and pathophysiological changes induced by treatment of experimental animals with sodium fluoride. Our review shows fluoride toxicosis caused an elevation in the serum activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, acid phosphatase, and the level of total bilirubin, and reduction in the serum levels of total protein, albumin, and globulins, and serious histopathological changes in the hepaic tissues. Also, NaF administration caused increases in serum urea, creatinine, uric acid, sodium ions, and chloride ions levels and serious histopathological changes in the kidney tissues. Treatment of experimental animals with NaF induced oxidative stress in hepatic and renal tissues. It can be concluded that administration of sodium fluoride to experimental animals induced oxidative stress, serious hepatorenal histopathological changes, and disturbance in liver and kidney functions. So, human should be advised to decrease exposure to sodium fluoride to decrease the harmful effects of NaF on liver and kidney.展开更多
AIM: To compare the effect of calcium and cholecalciferol alone and along with additional sodium fluoride or ibandronate on bone mineral density (BMD) and fractures in patients with Crohn's disease (CD). METHODS: ...AIM: To compare the effect of calcium and cholecalciferol alone and along with additional sodium fluoride or ibandronate on bone mineral density (BMD) and fractures in patients with Crohn's disease (CD). METHODS: Patients (n =148) with reduced BMD (T-score < -1) were randomized to receive cholecalciferol (1000 IU) and calcium citrate (800 mg) daily alone(group A, n = 32) or along with additional sodium fluoride (25 mg bid) (group B, n = 62) or additional ibandronate (1 mg iv/3-monthly) (group C, n = 54). Dual energy X-ray absorptiometry of the lumbar spine (L1-L4) and proximal right femur and X-rays of the spine were performed at baseline and after 1.0, 2.25 and 3.5 years. Fracture-assessment included visual reading of X-rays and quantitative morphometry of vertebral bodies (T4-L4).RESULTS: One hundred and twenty three (83.1%) patients completed the first year for intention-to-treat (ITT) analysis. Ninety two (62.2%) patients completed the second year and 71 (47.8%) the third year available for per-protocol (PP) analysis. With a significant increase in T-score of the lumbar spine by +0.28 ± 0.35 [95% conf idence interval (CI): 0.162-0.460, P < 0.01], +0.33 ± 0.49 (95% CI: 0.109-0.558, P < 0.01), +0.43 ± 0.47 (95% CI: 0.147-0.708, P < 0.01) in group A, +0.22 ± 0.33 (95% CI: 0.125-0.321, P < 0.01); +0.47 ± 0.60 (95% CI: 0.262-0.676, P < 0.01), +0.51 ± 0.44 (95% CI: 0.338-0.682, P < 0.01) in group B and +0.22 ± 0.38 (95% CI: 0.111-0.329, P < 0.01), +0.36 ± 0.53 (95% CI: 0.147-0.578, P < 0.01), +0.41 ± 0.48 (95% CI: 0.238-0.576, P < 0.01) in group C, respectively, during the 1.0, 2.25 and 3.5 year periods (PP analysis), no treatment regimen was superior in any in- or between-group analyses. In the ITT analysis, similar results in all in- and between-group analyses with a significant in-group but non-significant between-group increase in T-score of the lumbar spine by 0.38 ± 0.46 (group A, P < 0.01), 0.37 ± 0.50 (group B, P < 0.01) and 0.35 ± 0.49 (group C, P < 0.01) was observed. Follow-up in ITT analysis was still 2.65 years. One vertebral fracture in the sodium fluoride group was detected. Study medication was safe and well tolerated. CONCLUSION: Additional sodium fluoride or ibandronate had no benefit over calcium and cholecalciferol alone in managing reduced BMD in CD.展开更多
This study was carried out to observe the effects of sodium fluoride on acetylcholinesterase (AChE) activities in the cerebral synaptic membranes (SPM) and the peripheral red blood cells (RBC) of rats by in vivo and i...This study was carried out to observe the effects of sodium fluoride on acetylcholinesterase (AChE) activities in the cerebral synaptic membranes (SPM) and the peripheral red blood cells (RBC) of rats by in vivo and in vitro experiments. In the in vivo study,pregnant rats ingested ad libitum fluorinated drinking water (5, 15, 50 ppm F ) during their gestation and lactation. It was shown that the AChE activities of the SPM and peripheral RBCs in maternal rats exposed 5 ~ 50 ppm F for 60 days were elevated significantly by 30. 0 ~ 67. 6 % and 12. 5 ~ 31. 9 % in a dose-dependent manner, respectively. The AChE activities of their offspring 80 days after birth were also increaased (8. 7 ~ 28. 7 % for SPM and20. 6 ~ 32. 4 % for RBC). In contrast, the AChE activities of SPM in vitro were inhibited by 5. 0~ 50 .0 mmol F /L treatment in a time-and dose dependent manner. Analaysis with the Hanes plots suggested that the enzymesubstrate kinetics are consistent with a mixed type ofinhibition.展开更多
Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human...Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human ligament cells展开更多
目的分析;氟-氟化钠(^(18)F-Na F)正电子发射计算机断层显像/电子计算机X射线断层扫描技术(PET/CT)、;氟-脱氧葡萄糖(^(18)F-FDG)PET/CT及磁共振成像(MRI)对鼻咽癌患者颅底骨质受侵及骨转移的评估价值。方法收集48例经病理证实为鼻咽癌...目的分析;氟-氟化钠(^(18)F-Na F)正电子发射计算机断层显像/电子计算机X射线断层扫描技术(PET/CT)、;氟-脱氧葡萄糖(^(18)F-FDG)PET/CT及磁共振成像(MRI)对鼻咽癌患者颅底骨质受侵及骨转移的评估价值。方法收集48例经病理证实为鼻咽癌患者的临床资料,入院后均接受^(18)F-Na F PET/CT、^(18)F-FDG PET/CT、MRI检查,统计不同检查方法检出颅底骨质破坏及骨转移情况,比较三种方式对鼻咽癌诊断效能。结果^(18)F-Na F PET/CT、MRI诊断鼻咽癌颅底骨质受侵、骨转移效能均优于^(18)F-FDG PET/CT(P<0.05),但^(18)F-Na F PET/CT、MRI比较差异无统计学意义(P>0.05);骨质侵犯病灶分布:^(18)F-Na F PET/CT、^(18)F-FDG PET/CT、MRI检出颅底骨质受侵部位以枕骨斜坡受侵为主,检出率分别为84.62%(22/26)、69.23%(;/26)、80.77%(21/26);骨转移病灶:以椎体分布为主,其次为胸肋与骨盆。结论^(18)F-Na F PET/CT、MRI对鼻咽癌颅底骨质受侵、骨转移诊断效能优于^(18)F-FDG PET/CT。展开更多
文摘Osteoclasts are bone-specific multinucleated cells generated by the differentiation of monocyte/macrophage lineage precursors. Regulation of osteoclast differentiation is considered an effective therapeutic approach to the treatment of bone-lytic diseases. Periodontitis is an inflammatory disease characterized by extensive bone resorption. In this study, we investigated the effects of sodium fluoride （NaF） on osteoclastogenesis induced by Porphyromonas gingivalis, an important colonizer of the oral cavity that has been implicated in periodontitis. NaF strongly inhibited the P. gingivalis-induced alveolar bone loss. That effect was accompanied by decreased levels of cathepsin K, interleukin （IL）-1β, matrix metalloproteinase 9 （MMP9）, and tartrate-resistant acid phosphatase, which were up-regulated during P. gingivalis-induced osteoclastogenesis. Consistent with the in vivo anti-osteoclastogenic effect, NaF inhibited osteoclast formation caused by the differentiation factor RANKL （receptor activator of nuclear factor KB ligand） and macrophage colony-stimulating factor （M-CSF）. The RANKL-stimulated induction of the transcription factor nuclear factor of activated T cells （NFAT） cl was also abrogated by NaF. Taken together, our data demonstrate that NaF inhibits RANKL-induced osteoclastogenesis by reducing the induction of NFATcl, ultimately leading to the suppressed expression of cathepsin K and MMP9. The in vivo effect of NaF on the inhibition of P. gingivalis-induced osteoclastogenesis strengthens the potential usefulness of NaF for treating periodontal diseases.
基金supported by Jilin Provincial Science and Technology Development Project in China,20100407
文摘Objective To investigate the toxic effect of sodium fluoride（NaF） on the nematode Caenorhabditis elegans（C.elegans）.Methods Adult C.elegans were exposed to different concentrations of NaF（0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L） for 24 h.To assess the physiological effects of NaF,the brood size,life span,head thrashes,and body bend frequency were examined.Reactive oxygen species（ROS） and cell apoptosis were detected as parameters of biochemical response.The gene expressions were determined by real‐time polymerase chain reaction（PCR） to assess the molecular‐level response.Results At the physiological level,the brood size of C.elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 6%,26%,and 28% respectively in comparison with the control group.The maximum life spans of C.elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 3 days and 5 days,respectively.Head thrashes and body bend frequency both decreased with increasing concentrations of NaF.At the biochemical level,the production of ROS and the incidence of cell apoptosis increased with increasing concentrations of NaF（P0.05）.At the molecular level,different concentrations of NaF exposure raised the expression of stress‐related genes,such as hsp16.1,sod‐3,ctl‐2,dhs‐28,gst‐1,and cep‐1.Conclusion NaF exposure could induce multiple biological toxicities to C.elegans in a concentration‐dependent manner.These toxicities may be relevant to the oxidative stress induced by increased ROS production and accumulation in C.elegans.
文摘Sodium fluoride and high specific area silica were synthesized by using sodium hexafluorosilicate(Na2Si F6) and sodium carbonate decahydrate(Na2CO3·10H2O). The influencing factors of react temperature, contact time, sodium dodecyl sulfate(SDS) and molar ratio of Na2 Si F6 to Na2CO3·10H2O were investigated. The optimum process involves the reaction of 0.075 mol Na2 Si F6 and 150 m L, 0.225 mol Na2CO3·10H2O(molar ratio of 1:3) at 85 °C for 90 min, and 2.0×10-3 mol sodium dodecyl sulfate(SDS) as additive. The results show that the purities of Si O2 and Na F at extraction yields of 96.5% and 98.0% are 91.0% and 98.6%, respectively. The obtained Si O2 were characterized by X-ray diffraction(XRD), scanning electron microscope(SEM), Fourier transform infrared ray(FTIR), differential scanning calorimetry and thermogravimetric analysis(DSC-TGA), N2 absorption/desorption(BET) and laser particle size analyzer. The result demonstrates that Si O2 particles have a high BET surface area of 103 m2/g, and a mean grain size of 985 nm.
文摘A method is described for the direct determination of iron in high purity sodium fluoride using graphite furnace atomic absorption spectrometry. Interferences caused by the matrix are investigated. It is shown that the ashing temperature can be increased to 1 400°C and matrix interferences eliminated, the sensitivity of iron increased in 1. 27 fold by the addition of nickel nitrate. The method is applied to the determination of iron in sodium fluoride and satisfactory results are obtained.
基金This study was partial financially supported by Department of Physiology,Serampore College,West Bengal,India[Grant number SC/Physiol/PG/2018/017].
文摘Objective:To investigate the effect of Camellia(C.)sinensis in mitigating oxidative damage and reproductive toxicity in testis induced by sodium fluoride in a rat model.Methods:Twenty-four adult male Wister rats were divided into 4 groups,with 6 rats in each group.Group 1 orally received distilled water(1 mL/100 g body weight)daily and served as the control group,while group 2 received drinking water with 100 ppm sodium fluoride per day for 21 consecutive days,group 3 was administered with only C.sinensis extract by gavage at a dose of 100 mg/kg body weight and group 4 received drinking water with 100 ppm sodium fluoride and 100 mg/kg body weight C.sinensis leaf extract per day for 21 consecutive days.At the end of the treatment,the rats were sacrificed under light ether anesthesia.The gonado-somatic index,sperm count and motility,serum level of luteinizing hormone and testosterone were assayed.Lipid peroxidation[malondialdehyde(MDA)level],nitric oxide(NO)production,and activities of antioxidant enzymes-superoxide dismutase(SOD),catalase,and reduced glutathione level(GSH)were also analysed.Results:Sodium fluoride treatment significantly decreased gonado-somatic index,sperm count and motility as well as the serum level of luteinizing hormone and testosterone(P<0.05).The histological examination of testes revealed atrophy and degenerative changes in several seminiferous tubules,along with enhanced interstitial space and a reduced number of Leydig cells.There was a highly significant increase in NO and MDA production(P<0.05),while SOD,catalase activities and GSH level decreased significantly(P<0.05).However,C.sinensis significantly restored testicular weight,sperm parameter,hormonal level(P<0.05),and also reversed MDA and NO generation and antioxidant enzymes activities in the testicular tissue(P<0.05).Conclusions:C.sinensis may have an ameliorative role against sodium fluoride-induced oxidative damage in the testis probably because of its antioxidant property.
文摘The liver is a primary site for xenobiotics detoxification, and its metabolism is readily altered by toxicity. The kidney is a common target for toxic xenobiotics due to its capacity to extract and concentrate toxic substances by highly specialized cells. So, they are the target organs of sodium fluoride toxicity. The aim of this review is to highlight on hepatorenal oxidative stress and pathophysiological changes induced by treatment of experimental animals with sodium fluoride. Our review shows fluoride toxicosis caused an elevation in the serum activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, acid phosphatase, and the level of total bilirubin, and reduction in the serum levels of total protein, albumin, and globulins, and serious histopathological changes in the hepaic tissues. Also, NaF administration caused increases in serum urea, creatinine, uric acid, sodium ions, and chloride ions levels and serious histopathological changes in the kidney tissues. Treatment of experimental animals with NaF induced oxidative stress in hepatic and renal tissues. It can be concluded that administration of sodium fluoride to experimental animals induced oxidative stress, serious hepatorenal histopathological changes, and disturbance in liver and kidney functions. So, human should be advised to decrease exposure to sodium fluoride to decrease the harmful effects of NaF on liver and kidney.
文摘AIM: To compare the effect of calcium and cholecalciferol alone and along with additional sodium fluoride or ibandronate on bone mineral density (BMD) and fractures in patients with Crohn's disease (CD). METHODS: Patients (n =148) with reduced BMD (T-score < -1) were randomized to receive cholecalciferol (1000 IU) and calcium citrate (800 mg) daily alone(group A, n = 32) or along with additional sodium fluoride (25 mg bid) (group B, n = 62) or additional ibandronate (1 mg iv/3-monthly) (group C, n = 54). Dual energy X-ray absorptiometry of the lumbar spine (L1-L4) and proximal right femur and X-rays of the spine were performed at baseline and after 1.0, 2.25 and 3.5 years. Fracture-assessment included visual reading of X-rays and quantitative morphometry of vertebral bodies (T4-L4).RESULTS: One hundred and twenty three (83.1%) patients completed the first year for intention-to-treat (ITT) analysis. Ninety two (62.2%) patients completed the second year and 71 (47.8%) the third year available for per-protocol (PP) analysis. With a significant increase in T-score of the lumbar spine by +0.28 ± 0.35 [95% conf idence interval (CI): 0.162-0.460, P < 0.01], +0.33 ± 0.49 (95% CI: 0.109-0.558, P < 0.01), +0.43 ± 0.47 (95% CI: 0.147-0.708, P < 0.01) in group A, +0.22 ± 0.33 (95% CI: 0.125-0.321, P < 0.01); +0.47 ± 0.60 (95% CI: 0.262-0.676, P < 0.01), +0.51 ± 0.44 (95% CI: 0.338-0.682, P < 0.01) in group B and +0.22 ± 0.38 (95% CI: 0.111-0.329, P < 0.01), +0.36 ± 0.53 (95% CI: 0.147-0.578, P < 0.01), +0.41 ± 0.48 (95% CI: 0.238-0.576, P < 0.01) in group C, respectively, during the 1.0, 2.25 and 3.5 year periods (PP analysis), no treatment regimen was superior in any in- or between-group analyses. In the ITT analysis, similar results in all in- and between-group analyses with a significant in-group but non-significant between-group increase in T-score of the lumbar spine by 0.38 ± 0.46 (group A, P < 0.01), 0.37 ± 0.50 (group B, P < 0.01) and 0.35 ± 0.49 (group C, P < 0.01) was observed. Follow-up in ITT analysis was still 2.65 years. One vertebral fracture in the sodium fluoride group was detected. Study medication was safe and well tolerated. CONCLUSION: Additional sodium fluoride or ibandronate had no benefit over calcium and cholecalciferol alone in managing reduced BMD in CD.
文摘This study was carried out to observe the effects of sodium fluoride on acetylcholinesterase (AChE) activities in the cerebral synaptic membranes (SPM) and the peripheral red blood cells (RBC) of rats by in vivo and in vitro experiments. In the in vivo study,pregnant rats ingested ad libitum fluorinated drinking water (5, 15, 50 ppm F ) during their gestation and lactation. It was shown that the AChE activities of the SPM and peripheral RBCs in maternal rats exposed 5 ~ 50 ppm F for 60 days were elevated significantly by 30. 0 ~ 67. 6 % and 12. 5 ~ 31. 9 % in a dose-dependent manner, respectively. The AChE activities of their offspring 80 days after birth were also increaased (8. 7 ~ 28. 7 % for SPM and20. 6 ~ 32. 4 % for RBC). In contrast, the AChE activities of SPM in vitro were inhibited by 5. 0~ 50 .0 mmol F /L treatment in a time-and dose dependent manner. Analaysis with the Hanes plots suggested that the enzymesubstrate kinetics are consistent with a mixed type ofinhibition.
文摘Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human ligament cells
文摘目的分析;氟-氟化钠(^(18)F-Na F)正电子发射计算机断层显像/电子计算机X射线断层扫描技术(PET/CT)、;氟-脱氧葡萄糖(^(18)F-FDG)PET/CT及磁共振成像(MRI)对鼻咽癌患者颅底骨质受侵及骨转移的评估价值。方法收集48例经病理证实为鼻咽癌患者的临床资料,入院后均接受^(18)F-Na F PET/CT、^(18)F-FDG PET/CT、MRI检查,统计不同检查方法检出颅底骨质破坏及骨转移情况,比较三种方式对鼻咽癌诊断效能。结果^(18)F-Na F PET/CT、MRI诊断鼻咽癌颅底骨质受侵、骨转移效能均优于^(18)F-FDG PET/CT(P<0.05),但^(18)F-Na F PET/CT、MRI比较差异无统计学意义(P>0.05);骨质侵犯病灶分布:^(18)F-Na F PET/CT、^(18)F-FDG PET/CT、MRI检出颅底骨质受侵部位以枕骨斜坡受侵为主,检出率分别为84.62%(22/26)、69.23%(;/26)、80.77%(21/26);骨转移病灶:以椎体分布为主,其次为胸肋与骨盆。结论^(18)F-Na F PET/CT、MRI对鼻咽癌颅底骨质受侵、骨转移诊断效能优于^(18)F-FDG PET/CT。
