Expression and Purification of Hydrophilic Domains of Bovine Anion Exchanger,Member 1 and Electrogenic Sodium Bicarbonate Cotransporter 1

[ Objective] To express and purify the intracellular hydrophilic domains of bovine membrane carrier proteins：anion exchanger, member 1 （AE1） and electregenic sodium bicarbonate cotransporter 1 （NBCel）, which were associated with bicarbonate ion transport. [ Method] The hydrophilic domains of bovine AE1 and NBCel were amplified by PCR and inserted into the prokaryotic expression vector pET-28a, respectively. The recombinant plasmids were transformed into the expression strain E. coli BL21 （DE3） and then induced by IPTG. The expressed proteins were purified by nickel ion affinity chromatography and analyzed by 15% SDS-PAGE. [Result] The hydrophilic domains of bovine AE1 and NBCel were amplified respectively by PCR and expressed by prokaryotic expression system with the induction of IPTG. They were mainly expressed in the cyto- plasm of E. coli and high-purity was achieved by nickel ion affinity chromatography. [Condusion] The expression of the hydrophilic domains of bovine AE1 and NBCel provides a major exit route for preparation of antibodies and the regulatory mechanisms of carrier proteins.

Inhibition of the Na⁺/Ca²⁺Exchanger NCX₁Expressed in <i>Xenopus</i>Oocyte by Glycyrrhizic Acid and Cyclophylin A

The Na+/Ca2+ exchanger plays an important role in regulation of airway smooth muscle contraction by regulating intracellular calcium, and is a potential target for treatment of asthma. To test modulation of exchanger activity, we used Xenopus oocytes as model system. Na+/Ca2+ exchanger was expressed in the cells by microinjection of cRNA of the exchanger isoform NCX1. The activity of NCX1 was determined as Ni2+-sensitive current under voltage clamp in low Cl– medium and in the presence of the Cl–-channel inhibitor niflumic acid. Only this composition of solution allowed determining NCX1-mediated current with sufficient accuracy. Among a few tested Chinese herbal drugs, glycyrrhizic acid turned out to be a potent inhibitor of NCX1 with an apparent IC50 value of 40 μM. Previous work had revealed elevated cyclophylin A concentration in serum of asthmatic rats after receiving acupuncture treatment. Extracellular incubation of the oocytes in cyclophylin A for one day led to significant inhibition with an apparent IC50 value of about 1 μM. We suggest that effects of acupuncture and application of glycyrrhizic acid as an active constituent of Chinese medicine for treatment of asthma symptoms may partially be attributed to inhibition of the reversed mode of NCX1 and that these compounds may stimulate the search for new anti-asthmatic drugs.

Role of Na^+/H^+ exchanger isoform-1 in doxorubicin-induced multidrug-resistance HL-60 cell line

Objective: To explore the roles of intracellular pH value (pHi) and sodium-hydrogen exchanger isoform-1 (NHE-1) in the mechanism of multidrug resistance of leukemia cells. Methods: Multidrug resistant cell line HL-60 induced by doxorubicin(DOX) (called as HL-60/DOX cells) and their parent cell line HL-60 were employed as experiment group and control group. The proliferation and chemosensitivity of the cells were studied by MTT assay, and the expression of multidrug resistance protein (MRP) was detected by immol/Lunocytochemistry. Meanwhile, pHi was measured by spectrofluorometery with a fluorescence dye BCECF-AM. Based on the pHi recovery curve after intracellular acid loading, the activity of NHE-1 was analyzed. The expression of NHE-1 mRNA and MRP mRNA were determined by semi-quantitative RT-PCR. Cell apoptosis was observed with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and apoptotic DNA was extracted and electrophoresed. Results: ① The IC 50 values for DOX, MTZ, VCR and homoharringtonine(HT), in HL-60/DOX cells were significantly higher than those in HL-60 cells (P<0.01). HL-60/DOX cells expressed abundant MRP, but HL-60 cells did not. ② pHi of HL-60/DOX cells were significantly higher than that of HL-60 cells(P<0.001). The expression and activity of NHE-1 in HL-60/DOX cells were significantly stronger than those of HL-60 cells. ③After administration of the specific NHE-1 inhibitor dimethyl amiloride (DMA) at a certain range of concentrations, compared with HL-60 cells, the rate of growth inhibition of HL-60/DOX cells increased significantly (P<0.05), the drug-sensitivity of HL-60/DOX cells was significantly sensitive (P<0.01), the expression of MRP and MRP mRNA decreased significantly (P<0.01), the apoptosis rate increased significantly (P<0.01). Conclusion: NHE-1 is involved in the drug-resistant mechanisms of multidrug-resistant HL-60 cells induced by DOX. The specific NHE-1 inhibitor DMA can partly reverse the multidrug resistance of HL-60 cells induced by DOX.

Resuscitation from Prolonged Ventricular Fibrillation by Epinephrine Combined with Sodium-Hydrogen Exchanger Isoform-1 Inhibitor Cariporide

Objective To test the resuscitative effects from prolonged ventricular fibrillation by epinephrine combined with sodium hydrogen exchanger isoform 1 inhibitor Cariporide. Methods 16 rats were received a 3 mg/kg bolus of Cariporide or the same volume of 0.9%NaCl solution (control) 15 seconds before completion 12 minutes untreated VF. Chest compression (CC) was started for a total of 8 minutes. Adjusted the depth of compressor so that the aortic diastolic pressure to 25～28 mmHg during the 2nd minute of CC. Fix the depth of the piston and this depth was used throughout the remaining 6 minutes of CC. 10 seconds before starting the 3rd minute of chest compression, injected epinephrine (30 μg/kg). Recorded the time at which restoration of spontaneous circulation (ROSC) occurred in Cariporide treated rats. Electrical defibrillation was timed in control group to match the time of spontaneous defibrillation in Cariporide treated rats. To the rats, which cant be defibrillated spontaneously, received chest compression and rescues electrical shocks. Results compared with control group, with the same CC depth, Cariporide treated rats received the higher and longer lasting coronary perfusion pressure (P< 0.05), higher resuscitative rate (P< 0.05), less post resuscitative ventricular ectopic activities (P< 0.001), better hemodynamic effects and longer survival time (P< 0.05). Conclusion Epinephrine combined with sodium hydrogen exchanger isoform 1 inhibitor Cariporide may represent a novel and remarkably effective intervention for resuscitation from prolonged VF.

基于“1+1+X”导师团队指导模式的研究生创新能力培养体系构建与实践

研究生导师对学生的指导模式和指导成效是决定提高研究生创新能力的核心问题。该文以培养研究生创新能力为目标,以导师负责制与科研团队有机融合的“1+1+X”导师团队指导为模式,以高水平学科科研平台为支撑,以研究生定期学术交流制度化常态化为途径,以学术成果评价奖励为激励,构建导师团队、科研平台、学术交流和成果评价“四位一体”的研究生创新能力培养体系,破解研究生创新能力培养制约瓶颈。实践证明,“1+1+X”导师团队指导模式可激发研究生导师和学生的主观能动性,提高指导成效,能够培养出具备创新能力的高质量研究生。

Feedback regulation between phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 and transforming growth factor β1 and prognostic value in gastric cancer

BACKGROUND Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1(PREX1)was reported to be overexpressed in some cancers and involved in cancer development,but its expression and significance in gastric cancer remain unclear.AIM To evaluate the expression of PREX1 in gastric cancer and its significance in the development of gastric cancer,especially to evaluate the potential mechanism of PREX1 in gastric cancer.METHODS Bioinformatic analysis was performed in order to examine the expression of PREX1 in gastric cancer.The relationship between the survival rate of gastric cancer patients and PREX1 expression was assessed by Kaplan Meier portal.The Gene Set Enrichment Analysis and the correlation between PREX1 and transforming growth factor(TGF)β1 pathway-related mediators were evaluated by cBioPortal for Cancer Genomics.Western blotting and reverse transcriptase polymerase chain reaction assay were used to test the role of TGFβ1 on the expression of PREX1.Western blotting and dual-luciferase reporter system was used to evaluate the effect of PREX1 on the activation of TGFβ1 pathway.Wound healing and Transwell assay were used to assess the effect of PREX1 on the metastasis activity of gastric cancer cells.RESULTS PREX1 was overexpressed in the gastric tumors,and the expression levels were positively associated with the development of gastric cancer.Also,the high expression of PREX1 revealed poor prognosis,especially for those advanced and specific intestinal gastric cancer patients.PREX1 was closely involved in the positive regulation of cell adhesion and positively correlated with TGFβ1-related mediators.Furthermore,TGFβ1 could induce the expression of PREX1 at both the protein and mRNA level.Also,PREX1 could activate the TGFβ1 pathway.The induced PREX1 could increase the migration and invasion activity of gastric cancer cells.CONCLUSION PREX1 is overexpressed in gastric cancer,and the high level of PREX1 predicts poor prognosis.PREX1 is closely associated with TGFβsignaling and promotes the metastasis of gastric cancer cells.

未知人Na(+)-K(+)-Exchanging ATPase α1亚基基因第一内含子的获得及鉴定

目的研究人Ｎａ（＋）－Ｋ（＋）－ＥｘｃｈａｎｇｉｎｇＡＴＰａｓｅα１亚单位基因胞外区约８０～１３０位氨基酸编码序列的未知基因组结构。方法采用聚合酶链反应（ＰＣＲ）方法对人基因组ＤＮＡ及ｃＤＮＡ文库进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行分析。结果人基因组ＤＮＡ和ｃＤＮＡ经扩增后分别得到８３３和１９５ｂｐ两种不同大小的片段Ｆｇ，Ｆｃ。分析序列发现Ｆｇ与Ｆｃ相比在１３８～７７５ｂｐ处含有一６３８ｂｐ的插入片段，此片段与ＧｅｎＢａｎｋ中的任何已知序列均无明显同源性。结论本研究发现了一段全新未知的人Ｎａ（＋）－Ｋ（＋）－ＥｘｃｈａｎｇｉｎｇＡＴＰａｓｅα１亚单位基因的内含子全序列，并得到了第一外显子同第二外显子之间的相对位置和序列，其在Ｇｅｎ－Ｂａｎｋ的基因检索号为Ｌ７６９３８。序列分析表明该内含子可能具有潜在的调控基因表达的功能，并含有一个可能的编码序列。

Effect of 1-MCP on Gas Exchange and Carbohydrate Concentrations of the Cotton Flower and Subtending Leaf under Water-Deficit Stress

Ethylene is an endogenous plant hormone that increases under adverse environmental conditions, resulting in leaf and fruit abscission and ultimately yield reduction. In cotton, however, the effects of water-deficit stress on ethylene production have been uncertain. In this study it was hypothesized that application of an ethylene inhibitor 1-Methylcyclo- propene (1-MCP) would prevent ethylene production and result in alleviation of water-deficit stress consequences on the physiology and metabolism of the cotton flower and subtending leaf. To test this hypothesis, growth chamber experiments were conducted in 2009-2010 with treatments consisting of (C) untreated well-watered control, (C + 1MCP) well-watered plus 1-MCP, (WS) untreated water-stressed control, and (WS + 1MCP) water-stressed plus 1-MCP. The plants were subjected to two consecutive drying cycles during flowering, approximately 8 weeks after planting, and 1-MCP was foliar applied at a rate of 10g. ai/ha at the beginning of each drying cycle. The results showed that 1-MCP application had no significant effect on gas exchange functions and did not prevent reductions from water stress in leaf photosynthesis, respiration and stomatal conductance. However, application of 1-MCP resulted in a decrease in sucrose content of water-stressed pistils compared to the control indicating that 1-MCP has the potential to interfere in carbohydrate metabolism of reproductive units.

The rotational anisotropies in the ferromagnetism/antiferromagnetism 1/ antiferromagnetism 2 exchange bias structures

The rotational anisotropies in the exchange bias structures of ferromagnetism/antiferromagnetism 1/antiferro- magnetism 2 are studied in this paper. Based on the model, in which the antiferromagnetism is treated with an Ising mean field theory and the rotational anisotropy is assumed to be related to the field created by the moment induced on the antiferromagnetic layer next to the ferromagnetic layer, we can explain why in experiments for ferromag- netism （FM）/antiferromagntism 1 （AFM1）/antiferromagnetism 2 （AFM2） systems the thickness-dependent rotational anisotropy value is non-monotonic, i.e. it reaches a minimum for this system at a specific thickness of the first anti- ferromagnetic layer and exhibits oscillatory behaviour. In addition, we find that the temperature-dependent rotational anisotropy value is in good agreement with the experimental result.

瑞马唑仑调节EPAC1/RAP1信号通路对急性心肌梗死大鼠心肌损伤的影响

目的探讨瑞马唑仑调节环腺苷酸激活的交换蛋白1(EPAC1)/RAS相关蛋白1(RAP1)信号通路对急性心肌梗死(AMI)大鼠心肌损伤的影响。方法将大鼠按照随机数字表法分为假手术组、模型组、瑞马唑仑组、瑞马唑仑+8-CPT(EPAC1的激动剂)组,每组20只。除假手术组外,其余各组大鼠通过左前降支结扎法构建AMI大鼠模型;小动物超声仪检测心功能指标;HE染色检测心肌组织病理情况;化学比色法检测大鼠心肌组织超氧化物歧化酶(SOD)和丙二醛(MDA)水平;JC-1染色法检测大鼠心肌细胞线粒体膜电位;TUNEL染色检测心肌细胞TUNEL阳性率;Western blot检测心肌组织EPAC1、RAP1、胱天蛋白酶3(Caspase-3)蛋白表达水平。结果与假手术组相比,模型组大鼠心肌组织结构被严重破坏且浸润大量炎性细胞;心功能指标左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD),心肌组织MDA水平,心肌细胞TUNEL阳性率,心肌组织EPAC1、RAP1、Caspase-3蛋白表达水平均明显升高;左心室射血分数(LVEF)、左心室短轴缩短率(LVFS),心肌组织SOD水平,心肌细胞线粒体膜电位明显降低(P<0.05)。与模型组相比,瑞马唑仑组大鼠心肌损伤缓解,炎性细胞浸润减轻,心功能指标LVEDD、LVESD,心肌组织MDA水平,心肌细胞TUNEL阳性率,心肌组织EPAC1、RAP1、Caspase-3蛋白表达水平均明显降低;LVEF、LVFS,心肌组织SOD水平,心肌细胞线粒体膜电位明显升高(P<0.05)。EPAC1的激动剂减弱了瑞马唑仑对AMI大鼠心肌损伤的缓解作用。结论瑞马唑仑可能通过抑制EPAC1/RAP1信号通路抑制心肌细胞凋亡,减轻AMI大鼠心肌损伤。

Magnetic Ordering and Exchange Interaction of Laves Compounds Sm0.9Pr0.1(Fe1-xCox)2

Structure, magnetic properties and magnetostriction of Sm0.9Pr0.1(Fe1-xCox)2 compounds have been investigated by means of X-ray diffraction, a.c. initial susceptibility, extracting sample magnetometer, Mossbauer spec-troscopy and standard strain gauge techniques. The lattice parameter a of the MgCu2-type Laves compounds Sm0.9Pr0.1(Fe1-xCox)2 decreases nonlinearly with increasing Co concentration, deviating from the Vegard's law. Curie temperature Tc increases initially from 668 K for x=0 to 694 K for x=0.2 and then decreases to 200 K for x=1.0. The saturation magnetization Ms at temperatures 1.5 K, 77 K and 300 K have the same variation tendency as the composition dependence of Curie temperature, in consistence with rigid-band model. The easy magnetization direction (EMD) of Sm0.9Pr0.1(Fe1-xCox)2 lies along [111] direction in the range x<0.6, and changes to [110] for x=0.8, while Sm0.9Pr0.1Co2 stays in the paramagnetic state at room temperature. The composition dependence of the average hyperfine field,Hhf , demonstrates a similar variation tendency as that of the saturation magnetization Ms and Curie temperature Tc. The spontaneous magnetostricton Am increases with increasing Co content. The saturation magnetostriction λs decreases monotonically with increasing x, which is caused by the increase of magnetostriction constant λ100 with opposite sign to that of Am. A two-sublattice model has been proposed to understand the intermediate region between the [111] and [110] spin configurations, which can also be used to explain the temperature dependence of magnetization.

Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 is a diagnostic and prognostic biomarker for hepatocellular carcinoma

BACKGROUND Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1(PRex1)was reported to be a risk factor in several cancers,including breast cancer,lung cancer,and melanoma,but its expression and role in hepatocellular carcinoma(HCC)have not yet been fully studied.AIM To explore the expression of P-Rex1 in HCC,and further evaluate its potential application in the diagnosis and prognosis of HCC,especially in hepatitis B virus(HBV)-related patients.METHODS P-Rex1 expression in HCC was evaluated by real-time-quantitative polymerase chain reaction.The expression of P-Rex1 was subjected to correlation analysis with clinical features,such as lymph node invasion,distant metastasis,HBV infection,patient’s age and gender.Receiver operating characteristic analysis was used to examine the potential role of P-Rex1 as a diagnostic biomarker in HCC.Kaplan-Meier analysis was used to determine the association between P-Rex1 expression and overall survival,progression-free survival and relapse-free survival.Bioinformatic analysis was used to validate the clinical findings.RESULTS P-Rex1 expression was significantly increased in HCC tumors than adjacent tissues.The expression of P-Rex1 was higher in HCC patients with lymph node invasion,distant metastasis,HBV infection and positive alpha-fetoprotein,respectively.The receiver operating characteristic analysis showed that P-Rex1 was a diagnostic biomarker with a higher area under the curve value,especially in patients with HBV infection.Survival analysis showed that patients with higher P-Rex1 expression had a favorable survival rate,even in early-stage patients.CONCLUSION P-Rex1 expression was highly increased in HCC,and the expression level of PRex1 was positively correlated with tumor progression.P-Rex1 has a higher efficiency in the diagnosis of HBV-related HCC,and could also be used as a favorable prognostic factor for HCC patients.

USE OF CATION EXCHANGE RESIN IN SYNTHESIS OF N-SUBSTITUTED-1-AMINOALKANEPHOSPHONATE AND-PHOSPHINIC ACIDS

Strongly acidic cation exchange resin(1x1,H form)has been successfully used as a catalyst in synthesis of diphenyl N-benzyloxycarbonyl-1-aminobenzylphosphonate, N-benzyloxycarbonyl-1-aminobenzylphenylphosphinic acid and N-p-tolylsulfonyl-1-aminobenzyl phenylphosphinic acid in high yields.

冠心病病人血清KAL、ENOX1水平与冠状动脉斑块稳定性、预后的关系

目的:分析冠心病病人血清卡利他汀(KAL)、Ecto-NOX二硫化物硫醇交换器1(ENOX1)水平与冠状动脉斑块稳定性、预后的关系。方法:选取2021年1月—2022年2月我院收治的103例冠心病病人(作为冠心病组),根据冠状动脉斑块稳定性分为不稳定组(47例)和稳定组(56例);根据6个月后是否发生主要不良心血管事件分为预后不良组(34例)和预后良好组(69例)。另选取同期50名体检健康者(作为对照组)。采用酶联免疫吸附法检测血清KAL、ENOX1水平。采用Spearman法分析冠心病病人血清KAL与ENOX1水平的相关性;多因素Logistic回归分析血清KAL、ENOX1水平与冠心病病人冠状动脉斑块不稳定、预后不良的关系;受试者工作特征(ROC)曲线分析血清KAL、ENOX1水平对冠心病病人冠状动脉斑块不稳定和预后不良的预测价值。结果:冠心病组血清KAL水平低于对照组,ENOX1水平高于对照组(P<0.001)。不稳定组血清KAL水平低于稳定组,ENOX1水平高于稳定组(P<0.001)。预后不良组血清KAL水平低于预后良好组,ENOX1水平高于预后良好组(P<0.001)。Spearman相关性分析显示,冠心病病人血清KAL与ENOX1水平呈负相关(r=-0.780,P<0.001)。多因素Logistic回归分析显示,KAL升高为冠心病病人冠状动脉斑块不稳定和预后不良的独立保护因素,ENOX1升高为独立危险因素。ROC曲线分析显示,血清KAL、ENOX1水平联合预测冠心病病人冠状动脉斑块不稳定和预后不良的ROC曲线下面积高于各指标单独预测。结论:冠心病病人血清KAL水平降低,ENOX1水平升高与冠状动脉斑块不稳定、预后不良有关,二者联合对冠心病病人冠状动脉斑块稳定性和预后的预测价值较高。

禽白血病病毒J亚群感染鸡组织中细胞受体chNHE 1的表达分析

旨在研究鸡钠氢交换蛋白1(chicken sodium hydrogen exchanger isoform 1,chNHE1)在不同类型鸡组织内的表达情况,J亚群禽白血病病毒(subgroup J avian leukosis virus, ALV-J)的组织嗜性及ALV-J感染后组织chNHE1的mRNA变化情况。本研究利用qPCR技术,检测了商品肉鸡、商品蛋鸡和SPF鸡脏器内的chNHE1转录量,ALV-J感染鸡后各组织的病毒载量及组织chNHE1的转录量的变化。结果显示,chNHE1在3种类型鸡的心、肝、脾、肺、肾、盲肠扁桃体、胸腺和法氏囊中均能检测到,但转录量高低存在较大差异,其中,免疫器官内的chNHE1转录量相对较高;SPF鸡接种ALV-J后,心、脾、肾和盲肠扁桃体中的病毒载量相对较高,而肝、肺、胸腺和法氏囊中的病毒载量相对较低;ALV-J感染后,脾、肾和盲肠扁桃体中的chNHE1转录量明显上调,但心中的转录量与空白对照相比无明显变化。本研究分析了3种类型鸡chNHE1的组织转录特点及ALV-J感染对组织内chNHE1转录的影响,该研究结果为探究受体chNHE1的转录与ALV-J的组织嗜性的关系提供重要的理论依据。

鸟嘌呤核苷酸交换因子1与厚体1在胃癌发生发展及转移过程中的作用研究

目的检测鸟嘌呤核苷酸交换因子1(Vav1)、厚体1(Dkk1)在胃癌及癌旁组织中的表达水平,探讨Vav1和Dkk1在胃癌发生发展及转移过程中的作用及临床价值。方法选取2020年6月至2022年6月于内蒙古科技大学包头医学院第一附属医院外科行胃癌根治术且经过病理确诊的57例胃癌患者作为研究对象,收集患者胃癌组织、癌旁组织(癌组织边缘5 cm)各57份。应用免疫组化法检测Vav1和Dkk1在胃癌及癌旁组织中的表达,分析胃癌中Vav1与Dkk1阳性表达率的相关性,不同临床病理特征与Vav1和Dkk1表达情况的关系,ROC曲线分析Vav1和Dkk1在胃癌中的诊断价值。结果胃癌组织中Vav1和Dkk1的阳性表达均高于癌旁组织,差异有统计学意义(P<0.05)。Vav1和Dkk1在癌旁组织很少表达,Vav1和Dkk1在胃癌组织中表达于细胞质,镜下可见胞质中散在黄色或黄褐色染色的颗粒。Vav1阳性与阴性患者肿瘤分期、淋巴结转移、肿瘤直径比较差异有统计学意义(P<0.05),性别、年龄、肿瘤部位、分化程度比较差异无统计学意义。Dkk1阳性与阴性患者分化程度、肿瘤分期、淋巴结转移和肿瘤直径比较差异有统计学意义(P<0.05),性别、年龄、肿瘤部位比较差异无统计学意义。胃癌中Vav1与Dkk1阳性表达率呈正相关(r>0,P<0.05)。Vav1和Dkk1的AUC分别为0.952和0.857,对胃癌的诊断效能均较理想。结论Vav1、Dkk1在胃癌中的表达呈正相关。Vav1和Dkk1在胃癌发生发展及转移过程中起重要作用,Vav1、Dkk1特异性靶向药物有望为胃癌有效治疗提供新思路。

Exchange Server2010只能安装在Windows Server2008R2上，这是真的吗？Windows Server2008R2支持Exchange Server2007P~？

Windows Server 2008 SP2 64位版本和Windows Server 2008 R2都支持Exchange Server2010。 Windows Server 2008 R2不支持Exchange Server2007和Exchange Server 2007 SP1。Windows Server 2008 R2预计也不会增加对Exchange Server 2007 SP2的支持。

Exchange Server 2007 SP1纵览

随着统一消息功能的改进和开始支持Windows Server 2008,Exchange Server 2007

Exchange Server 2007 SP1备用持续复制功能

使用Exchange Server 2007 SP1实现SCR功能。能够为你的Exchange组织增加站点弹性。了解建立SCR的步骤以及在失败事件中如何进行恢复。

微软新的竞争模型？Exchange Server 2007 SP1和PerformancePoint

每当微软意识到挑战的时候，它那变革的车轮便以惊人的速度转动起来，其效率远比政客的攻关团队在一次丑陋的政治表演后收拾残局的速度高得多。微软当前正在全力应对的一个挑战就是大众对于微软无法与Google以及“软件即服务（SaaS）”展开竞争的普遍认识。