Two strategies have been developed to improve DNA recovery in terms of yield,purity and unbiased representation of the microbial diversity.However,amplification of DNA from soil is often inhibited by co-purified conta...Two strategies have been developed to improve DNA recovery in terms of yield,purity and unbiased representation of the microbial diversity.However,amplification of DNA from soil is often inhibited by co-purified contaminants.Furthermore,DNA is also suitable for PCR amplification using various DNA targets.This review presents an overview of the available methods to achieve this challenging objective.DNA was extracted from 100 g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation,polyethylene glycol precipitation,phenol extraction and isopropanol precipitation.展开更多
On the basis of ecological principles including holistic optimization, cycling and regeneration, and regional differentiation, land treatment systems (LTSs) for municipal wastewater were continuously explored and upda...On the basis of ecological principles including holistic optimization, cycling and regeneration, and regional differentiation, land treatment systems (LTSs) for municipal wastewater were continuously explored and updated in the western Shenyang area and the Huolinhe area, China. Intensified pretreatment, addition of a man-made soil filtration layer, and use of an ecologically diversified secondary plant cover were proved to be technically feasible. Hydraulic loading was determined according to the assimilation capacity of soil ecosystems, thus ensuring safe operation of wastewater treatment. This modernized and alternative approach to wastewater treatment had been widely applied in middle-sized and small cities and towns of Northeast China, and these innovative systems in some areas had indicated favorable ecological, social, and economic benefits.展开更多
基金We thank Semnan University Department of Desert Science for supplying soil from Semnan Agriculture Station and Semnan University.
文摘Two strategies have been developed to improve DNA recovery in terms of yield,purity and unbiased representation of the microbial diversity.However,amplification of DNA from soil is often inhibited by co-purified contaminants.Furthermore,DNA is also suitable for PCR amplification using various DNA targets.This review presents an overview of the available methods to achieve this challenging objective.DNA was extracted from 100 g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation,polyethylene glycol precipitation,phenol extraction and isopropanol precipitation.
基金Project supported by the National Natural Science Founds for Distinguished Young Scholars, China (No. 20225722)the Key Programme of the National Natural Science Foundation of China (No. 20337010)
文摘On the basis of ecological principles including holistic optimization, cycling and regeneration, and regional differentiation, land treatment systems (LTSs) for municipal wastewater were continuously explored and updated in the western Shenyang area and the Huolinhe area, China. Intensified pretreatment, addition of a man-made soil filtration layer, and use of an ecologically diversified secondary plant cover were proved to be technically feasible. Hydraulic loading was determined according to the assimilation capacity of soil ecosystems, thus ensuring safe operation of wastewater treatment. This modernized and alternative approach to wastewater treatment had been widely applied in middle-sized and small cities and towns of Northeast China, and these innovative systems in some areas had indicated favorable ecological, social, and economic benefits.
