The effects of soil water regime and soil reaction on nitrogen distribution and fractional composition of newly-formed humus (decayed products) and the structural characteristics of the newly-formed humic acids (HAs) ...The effects of soil water regime and soil reaction on nitrogen distribution and fractional composition of newly-formed humus (decayed products) and the structural characteristics of the newly-formed humic acids (HAs) were studied in an incubation experiment, with the following results obtained:1. The humus newly formed under submerged conditions was higher in the relative content of α-amino acid-N and the humic acid/ fulvic acid (HA/ FA) ratio than that under upland conditions. The HAs extracted from the former were also higher in C / O ratio, aromaticity and the contents of methoxyl groups and lignin-like components, but lower in the content of carboxyl groups than the HAs from the latter.2. Under upland conditions, the C / organic N ratio and the relative content of mobile HA of newly-formed humus were lower, but the HA / FA ratio was higher in the CaCO3-amended treatment than in non-amended treatment. The presence of CaCO3 also resulted in a decrease of C / N ratio of HA and a slight increase of its carboxyl group content. On the other hand, there was no significant change in the composition and properties of the newly-formed humus with the addition of CaCO3 under submerged conditions.3. Compared with soil HAs, the newly-formed HAs contained more carbohydrates, polypeptides and lignin-like components, and were in lower degrees of oxidation and humification.展开更多
This paper conducts laboratory tests to investigate detailedly the soil deformation law around the pipeline and its penetration depth under self-gravity. The seabed model is prepared by consolidating saturated soil us...This paper conducts laboratory tests to investigate detailedly the soil deformation law around the pipeline and its penetration depth under self-gravity. The seabed model is prepared by consolidating saturated soil using vacuum pressure technology, and the pipeline models are specifically designed to possess different radii. Based on the experimental results and digital images, the soil deformation process is analyzed and summarized, a kinematic admissible velocity field is given and an upper bound solution of pipeline penetration depth and soil reaction force is derived and proposed in this paper. In order to verify the accuracy of the upper bound solution deduced in this paper,a comparison is made among some published results and the solution suggested in this paper, the comparison results confirm that the upper bound solution and the soil failure mode are reasonable. Finally two empirical formulas are given in this paper to estimate the soil reaction force of seabed and the penetration depth of pipeline. The empirical formulas are in agreement with the upper bound solution derived in this paper, and the conclusion of this paper could provide some theoretical reference for the further study of the interaction between the pipeline and the soil.展开更多
Factors including Mg^2＋, dNTP and primer that affected RAPD were studied using orthogonal experimental design, and at the same time, anneal temperature, extending time and cycles were also studied. Finally, PCR react...Factors including Mg^2＋, dNTP and primer that affected RAPD were studied using orthogonal experimental design, and at the same time, anneal temperature, extending time and cycles were also studied. Finally, PCR reaction system that is feasible for black soil microbial PCR amplification was determined, containing 7 ng DNA template, 20 pM random primers, 1.5 U Taq DNA polymerase, 3.0 mM MgCl2 and 0.2 mmol ·L^-1 dNTP, with procedure： 40 cycles for 3 min at 94 ℃ （temperature）, 40 s at 37 ℃ （annealing）, 1.5 min at 72 ℃ （extension）, a final elongation step at 72 ℃ for 7 min.展开更多
The ideal reducing reagent in testing available nitrogen of soil by Conway Methodis Devard‘s alloy.The optimum ratio of soil:Devard’s alloy:1.0N NaOH solution is 5.0g:0.2g:5ml.When reaction goes on at 30℃ for 4 hou...The ideal reducing reagent in testing available nitrogen of soil by Conway Methodis Devard‘s alloy.The optimum ratio of soil:Devard’s alloy:1.0N NaOH solution is 5.0g:0.2g:5ml.When reaction goes on at 30℃ for 4 hours,the rate of soil NO<sub>3</sub>-N reduction is about 90%,and the recovery rate of soil NH<sub>4</sub>-N is about 95%.Some organic nitrogen can be measured at thesame time.This method is defined as the Modified Conway Method and the soil N-min is r=0.982（n=10）.In predicting soil nitrogen supplying power,the Modified Conway Method takesonly 1/6 of time needed by Conway Method.The Modified Conway Method can also be used forthe measure of soil N-min.展开更多
Fluorine contaminates the environment. The study of fluorine contamination profile can be made easy by the use of nuclear analytic method. Measurement of prompt gamma emitted from bombarding fluorine polluted environm...Fluorine contaminates the environment. The study of fluorine contamination profile can be made easy by the use of nuclear analytic method. Measurement of prompt gamma emitted from bombarding fluorine polluted environmental sample with proton beam from accelerator provides rapid assessment of fluorine contamination. In this paper, 340 keV proton beam induced F 19 (P,αγ) O 16 reaction is performed, measurement of prompt gamma 6130 keV gives fluorine content in the soil and leaves of plants (parasol, cotton and glossy privet), taken from the fluorine polluted area.展开更多
In this paper, the flexural behavior of laterally loaded tapered piles in cohesive soils is investigated. The exact solution for the governing differential equation of the problem is obtained based on the beam-on-elas...In this paper, the flexural behavior of laterally loaded tapered piles in cohesive soils is investigated. The exact solution for the governing differential equation of the problem is obtained based on the beam-on-elastic foundation approach in which the soil reaction on the pile is related directly to the pile lateral deflection. In this investigation, the modulus of subgrade reactions is assumed to be constant along the pile depth. Parametric study through numerical examples is carried out to prove the validity and accuracy of the obtained results. In general, the derived displacement field can be used to study pile response in multilayered soil profiles by subdividing the pile into a number of elements. It is found that tapered piles show stiffer behavior than that for prismatic ones having the same material volume with an optimum stress distribution along the pile depth. Accordingly, tapered piles are more efficient and economic than those having the same material volume. Verification is also carried out for the obtained results through finite element analysis and the selected number of elements gives a very good agreement for lateral deflection and a larger number of elements is required to obtain better results for bending moment because of moment loss resulting from the lack of shear diagram.展开更多
文摘The effects of soil water regime and soil reaction on nitrogen distribution and fractional composition of newly-formed humus (decayed products) and the structural characteristics of the newly-formed humic acids (HAs) were studied in an incubation experiment, with the following results obtained:1. The humus newly formed under submerged conditions was higher in the relative content of α-amino acid-N and the humic acid/ fulvic acid (HA/ FA) ratio than that under upland conditions. The HAs extracted from the former were also higher in C / O ratio, aromaticity and the contents of methoxyl groups and lignin-like components, but lower in the content of carboxyl groups than the HAs from the latter.2. Under upland conditions, the C / organic N ratio and the relative content of mobile HA of newly-formed humus were lower, but the HA / FA ratio was higher in the CaCO3-amended treatment than in non-amended treatment. The presence of CaCO3 also resulted in a decrease of C / N ratio of HA and a slight increase of its carboxyl group content. On the other hand, there was no significant change in the composition and properties of the newly-formed humus with the addition of CaCO3 under submerged conditions.3. Compared with soil HAs, the newly-formed HAs contained more carbohydrates, polypeptides and lignin-like components, and were in lower degrees of oxidation and humification.
基金financially supported by the National Natural Science Foundation of China(Grant No.51679224)
文摘This paper conducts laboratory tests to investigate detailedly the soil deformation law around the pipeline and its penetration depth under self-gravity. The seabed model is prepared by consolidating saturated soil using vacuum pressure technology, and the pipeline models are specifically designed to possess different radii. Based on the experimental results and digital images, the soil deformation process is analyzed and summarized, a kinematic admissible velocity field is given and an upper bound solution of pipeline penetration depth and soil reaction force is derived and proposed in this paper. In order to verify the accuracy of the upper bound solution deduced in this paper,a comparison is made among some published results and the solution suggested in this paper, the comparison results confirm that the upper bound solution and the soil failure mode are reasonable. Finally two empirical formulas are given in this paper to estimate the soil reaction force of seabed and the penetration depth of pipeline. The empirical formulas are in agreement with the upper bound solution derived in this paper, and the conclusion of this paper could provide some theoretical reference for the further study of the interaction between the pipeline and the soil.
基金This Work was Supported by the National Natural Science Foundation of China (30230250, 30370819) Heilongjiang ProvinceScience Foundation (C0215)
文摘Factors including Mg^2＋, dNTP and primer that affected RAPD were studied using orthogonal experimental design, and at the same time, anneal temperature, extending time and cycles were also studied. Finally, PCR reaction system that is feasible for black soil microbial PCR amplification was determined, containing 7 ng DNA template, 20 pM random primers, 1.5 U Taq DNA polymerase, 3.0 mM MgCl2 and 0.2 mmol ·L^-1 dNTP, with procedure： 40 cycles for 3 min at 94 ℃ （temperature）, 40 s at 37 ℃ （annealing）, 1.5 min at 72 ℃ （extension）, a final elongation step at 72 ℃ for 7 min.
文摘The ideal reducing reagent in testing available nitrogen of soil by Conway Methodis Devard‘s alloy.The optimum ratio of soil:Devard’s alloy:1.0N NaOH solution is 5.0g:0.2g:5ml.When reaction goes on at 30℃ for 4 hours,the rate of soil NO<sub>3</sub>-N reduction is about 90%,and the recovery rate of soil NH<sub>4</sub>-N is about 95%.Some organic nitrogen can be measured at thesame time.This method is defined as the Modified Conway Method and the soil N-min is r=0.982（n=10）.In predicting soil nitrogen supplying power,the Modified Conway Method takesonly 1/6 of time needed by Conway Method.The Modified Conway Method can also be used forthe measure of soil N-min.
文摘Fluorine contaminates the environment. The study of fluorine contamination profile can be made easy by the use of nuclear analytic method. Measurement of prompt gamma emitted from bombarding fluorine polluted environmental sample with proton beam from accelerator provides rapid assessment of fluorine contamination. In this paper, 340 keV proton beam induced F 19 (P,αγ) O 16 reaction is performed, measurement of prompt gamma 6130 keV gives fluorine content in the soil and leaves of plants (parasol, cotton and glossy privet), taken from the fluorine polluted area.
文摘In this paper, the flexural behavior of laterally loaded tapered piles in cohesive soils is investigated. The exact solution for the governing differential equation of the problem is obtained based on the beam-on-elastic foundation approach in which the soil reaction on the pile is related directly to the pile lateral deflection. In this investigation, the modulus of subgrade reactions is assumed to be constant along the pile depth. Parametric study through numerical examples is carried out to prove the validity and accuracy of the obtained results. In general, the derived displacement field can be used to study pile response in multilayered soil profiles by subdividing the pile into a number of elements. It is found that tapered piles show stiffer behavior than that for prismatic ones having the same material volume with an optimum stress distribution along the pile depth. Accordingly, tapered piles are more efficient and economic than those having the same material volume. Verification is also carried out for the obtained results through finite element analysis and the selected number of elements gives a very good agreement for lateral deflection and a larger number of elements is required to obtain better results for bending moment because of moment loss resulting from the lack of shear diagram.
