Halobacterium Identification in Saltworks of Gran Canaria (Canary Islands, Spain)

This work analyzes bacterial communities present in evaporation ponds of solar salterns of Gran Canaria and reveals specific organisms through molecular techniques.Solar salterns are protected areas in Canary Islands where salt is produced from sea water by solarand windpowered evaporation.Salt was an important product for ancient islanders who used it for a broad field of purposes,but also has a great importance in recent time for its implications in the island economy.Based on amplifications with specific primers for 16S ribosomal DNA(16S rDNA)and subsequent nested-PCR approaches,different amplicons were obtained,and analyzed in silico.A taxonomic classification was carried out through phylogenetic trees.Results revealed different bacteria according to the evaporation grade of crystallizer ponds in saline works.It is worthstanding the presence of the genus Halobacterium in all crystallizer ponds.This opens an interesting framework for further studies and continuative molecular characterization approaches of bacterial communities in solar salterns of Gran Canaria.

淡化浓海水日晒盐田原核微生物多样性分析

海滨日晒盐田具有稳定且独特的生态系统。淡化浓海水排入盐田制盐,对盐田生态系统的稳定及盐田的可持续发展具有一定的影响。本研究分析天津汉沽淡化浓海水日晒盐田不同季节不同盐度盐池原核微生物多样性现状及群落结构变化,以期为评估淡化浓海水对盐田原核微生物群落影响提供理论依据。采用温度梯度凝胶电泳和序列测定等方法,分析不同月份不同盐度盐池中细菌和古菌的多样性与群落结构变化。分别采用香农-威纳指数、加权丰富度指数和均匀度指数分析盐田微生物群落多样性、丰富度和均匀度。结果表明,淡化浓海水和溴后水中均未检测出细菌和古菌。不同月份不同盐度盐池样本中细菌多样性变化不大,优势菌主要为γ-变形菌门(γ-Proteobacteria)中的Spiribacter salinus和Pseudoalteromonas sp.;古菌仅出现在盐度最高的Ⅶ号盐池中,优势古菌为广古菌门(Euryarchaeota)的Halogeometricum sp.。天津汉沽淡化浓海水日晒盐田中细菌的多样性和丰富度较低,春夏两季细菌群落结构较为稳定,秋冬季节细菌群落结构变化明显。盐度不同的盐池中细菌群落结构变化较大,随着盐度逐渐升高,Spiribacter salinus逐渐取代Pseudoalteromonas sp.成为占主要地位的优势菌种。