Local injection of bortulinum toxin A at multiple points for decreasing the muscular tension of children with spastic cerebral palsy A comparative observation

More than 70% children with cerebral palsy belong to the spastic one, and the increased muscular tension results in motor dysfunction and posture abnormality. OBJECTIVE： To observe the assistant effect of the local injection of BTX-A at multiple points in the rehabilitative treatment of spastic cerebral palsy. DESIGN： A comparative observation on the clinical efficacy. SETTINGS： Rehabilitation Center, Loufeng Hospital, Suzhou Industrial Park; Gansu Rehabilitation Center Hospital. PARTICIPANTS： Sixty-nine children with cerebral palsy were selected from the outpatients and inpatients who accepted rehabilitative treatments in the Cerebral Palsy Treatment Center of Gansu Rehabilitation Center Hospital from April 2003 and October 2004. Inclusive criteria： ①Having high risk factors for brain injury before birth and within 1 month after birth, mainly manifested as central motor disorder and posture abnormality; ② Accompanied by increase of muscular tension in both lower limbs, appearing as tiptoes and scissors gaits while in standing position or walking; ③ Without fixed contracture of lower limb; ④ Had never been operated for lower limbs; ⑤ Had never been injected with BTX-A; ⑥ Clearly diagnosed to have spastic palsy. The enrolled children were not screened by disease conditions, course, age, etc., and informed consents were obtained from the parents of all the children. The children whose parents agreed to accept BTX-A treatment were taken as BTX-A treated group （n =45）, and the others as control group （n = 24）. METHODS： All the children accepted regular rehabilitative training after admission, 6 times a weeks, besides, those in the BTX-A treated group were given local injection of BTX-A at multiple points of target muscles, including bilateral adductor longus muscles, pectineal muscles, gastrocnemius muscles and soleus muscles. BTX-A was diluted with saline （5 U BTX-A in 0.1 mL）, 3 - 4 U/kg for each piece of muscle. The number of injected sites depended on the size of target muscle, and no more than 0.5 mL for each site. The injection should be followed by manual massage for 1 - 2 minutes, and electromuscular stimulation for 20 minutes, which were continued for 3 days. They participated in the comprehensive rehabilitative training and treatment at 24 hours after injection. MAIN OUTCOME MEASURES： The comprehensive abilities of cerebral palsy were evaluated, the adductor angle and forced area on soles were measured before treatment and at 3 and 6 months after treatment. RESULTS： All the 69 children with spastic cerebral palsy were involved in the analysis of results. ① Efficacy in the BTX-A treated group： BTX-A began to take effect at 48 hours after injection, and the maximal effect was observed in all the cases on the 6th day; 2 weeks later, the scissors gaits and hip adduction were obviously improved in 39 and 11 cases, respectively, and tiptoes gaits were improved in 22 cases; 2 months later, tiptoes gaits were improved in 43 cases. ② Efficacy in the control group： Two months later, certain improvements of scissors gaits were observed in 2 children with mild cerebral palsy; the scissors gaits and tiptoes were obviously improved in 6 cases of moderate cerebral palsy and 3 cases of mild one after 3 months, and there were changes of the parameters after 6 months in 1, 10 and 5 cases of severe, moderate and mild cerebral palsy, respectively. ③ Improvement of adductor angle before and after treatment in both groups： The muscular tension was evaluated according to Komam＇s method. There was no obvious difference in the adductor angle between the two groups before treatment （P 〉 0.05）. The adductor angle had very significant difference （P 〈 0.01） and significant difference （P 〈 0.05） between the two groups at 3 and 6 months after treatment. ④ Changes of forced area on soles before and after treatment in bothgroups： The forced area on soles was assessed referred to the method of foot-print analysis. There was no obvious difference in the forced area on soles between the two groups before treatment （P 〉 0.05）. The forced area on soles had very significant difference （P 〈 0.01） and significant difference （P 〈 0.05） between the two groups at 3 and 6 months after treatment. CONCLUSION： BTX-A local injection is an important assistant therapeutic method for treating spastic cerebral palsy. For the patients with moderate and severe cerebral palsy, BTX-A local injection should be combined with rehabilitative training to obviously shorten the course and improve efficacy.

Concerns about Diabetic Foot Wounds

This paper in a medical sense can be described as a case report. The diabetic foot wounds of a relative, over 70 years of age, with Type I diabetes were so grievous and the medical treatment was so protracted that success could not be assured. There was a danger of amputation. The one outstanding feature was that the private application of an antifungal spray, between the toes of both feet without prescribed oral mycologic agents, seemed to make an inexplicable difference to the final outcome. In addition, a viewpoint drawn was that the efficacy of antibiotic therapies may be compromised by the presence of invisible fungal etiology.

Molecular cloning,characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole(Cynoglossussemilaevis)

Two transcription factors, Sox9a and Foxl2 were cloned from half-smooth tongue sole （Cynoglossus semilaevis）. Sox9a is a new duplication of C.semilaevis Sox9 gene. The complete cDNA of Sox9a gene was 1 842 bp long coding for 487 amino acids and Foxl2 gene was 1 817 bp coding for 308 amino acids. Sox9a was expressed higher in male brain, pituitary and gonad and Foxl2 were higher in female brain, pituitary and gonad. The expression of Sox9a gene in gonads of neo-males was higher than that of normal females. Sox9a and Foxl2 were expressed higher in gastrula stage than in other stages. In the period of sex differentiation, the expression of Sox9a was first going up and then going down and Foxl2 was higher expressed at 37 dph. The highest expressions of Sox9a and Foxl2 genes occurred in nine-month and 12-month old gonad tissues, respectively. Sox9a gene was considered to have inevitable links with sex reversal, sex differentiation and cell differentiation of embryos and formation of spermatogenic ceils. Foxl2 was considered to play a role in sex differentiation, cell differentiation of embryos but not to be necessary for sex determination and sex reversal.

Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)

A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole （Cynoglossus sernilaevis）. CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS （fetal bovine serum）, 2.5 ng/cm^3 bFGF （basic fibroblast growth factor）, 1 ng/cm^3 LIF （leukemia inhibitory factor） and 50 mmol/dm^3 2-ME （2-mecaptoethanol）. The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24 ℃. FBS and bFGF concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect （CPE） after infection with lymphosystis disease virus （LCDV） in 2 d. Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus.

Analysis of DNA Methylation Level by Methylation-Sensitive Amplification Polymorphism in Half Smooth Tongue Sole(Cynoglossus semilaevis)Subjected to Salinity Stress

Increasingly arisen environmental constraints may contribute to heritable phenotypic variation including methylation changes,which can help the animals with development,growth and survival.In this study,we assessed the DNA methylation levels in three tissues(gonad,kidney and gill) of half smooth tongue sole under the salinity stress.The methylation-sensitive amplification polymorphism(MSAP) technique was applied to illustrate the regulation of epigenetic mechanism in environmental stimuli.Fish were subjected to 15 salinity treatment for 7 and 60 days,respectively.A total of 11259 fragments were amplified with 8 pairs of selective primers.The levels of methylated DNA in different tissues of females and males without salinity stress were analyzed,which were 32.76% and 47.32% in gonad;38.13% and 37.69% in kidney;37.58% and 34.96% in gill,respectively.In addition,the significant difference was observed in gonad between females and males,indicating that discrepant regulation in gonadal development and differentiation may involve sex-related genes.Further analysis showed that total and hemi-methylation were significantly decreased under 15 salinity for 7 days,probably resulting in up-regulating salt-tolerance genes expression to adjust salt changing.With the adjustment for 60 days,total and hemi-methylation prominently went back to its normal levels to obtain equilibrium.Particularly,full methylation levels were steady along with salinity stress to maintain the stability of gene expression.Additionally,the data showed that gonads in females and gills in males were superior in adaptability.As a result,DNA methylation regulates tissue-specific epiloci,and may respond to salinity stress by regulating gene expression to maintain animal survival and activity.

Nitrogen uptake and transfer in broad bean and garlic strip intercropping systems

Utilization and transfer of nitrogen （N）in a strip intercropping system of garlic （Allium safivum L.） and broad bean （Vicia faba L.） have been investigated rarely. The objectives of this study were to quantify N uptake and utilization by intercropped broad bean and garlic and determine the magnitude of N transfer from broad bean to garlic. Field and pot trials were carried out in the Erhai Lake Basin in China using ^15N tracer applied to the soil or injected into broad bean plants. Strip intercropping of garlic and broad bean increased N absorption （47.2%） compared with sole crop broad bean （31.9%） or sole crop garlic （40.7%） and reduced soil residual N. Nearly 15% of 15N injected into petioles of broad bean intercropped with garlic was recovered in garlic at harvest, suggesting that N could be transferred from broad bean to strip intercropped garlic. The findings provide a basis for evaluating legumes＇ role in optimizing N fertilization when intercropped with non-legumes.

Changes of Soil Microbiological Characteristics After Solidago canadensis L. Invasion

The relationship between Solidago canadensis L. invasion and soil microbial communities was studied across the invasive gradients varying from 0 to 40, 80, and 100% coverage of S. canadensis. The results showed both soil microbial biomass C （Cmic） and N （Nmic） increased as the coverage of S. canadensis increased. Soil microbial quotient Cmic/Corg （microbial biomass C/organic C） tended to increase linearly with the coverage of S. canadensis. Soil basal respiration （BR） also showed a similar trend. The soil respiratory quotient qCO2 decreased with S. canadensis invasion, and remained at quite a constantly low level in the invasive soils. Sole carbon source utilization profiles analyses indicated that S. canadensis invasion tended to result in higher microbial functional diversity in the soil. Average utilization of specific substrate guilds was highest in the soil with S. canadensis monoculture. Principle component analysis of sole carbon source utilization profiles further indicated that microbial functional diversity in the soil with S. canadensis monoculture was distinctly separated from those soils in the native area and the ecotones. In conclusion, S. canadensis invasion improved soil microbial biomass, respiration and utilization of carbon sources, and decreased qCO2, thus created better soil conditions, which in turn were more conducive to the growth of S. canadensis.

Geochronological constraints on the metamorphic sole of the Semail ophiolite in the United Arab Emirates

The Semail ophiolite of Oman and the United Arab Emirates （UAE） provides the best preserved large slice of oceanic lithosphere exposed on the continental crust, and offers unique opportunities to study processes of ocean crust formation, subduction initiation and obduction. Metamorphic rocks exposed in the eastern UAE have traditionally been interpreted as a metamorphic sole to the Semail ophiolite. However, there has been some debate over the possibility that the exposures contain components of older Arabian continental crust. To help answer this question, presented here are new zircon and futile U-Pb geochronological data from various units of the metamorphic rocks. Zircon was absent in most samples. Those that yielded zircon and futile provide dominant single age populations that are 95-93 Ma, partially overlapping with the known age of oceanic crust formation （96.5-94.5 Ma）, and partially overlapping with cooling ages of the metamorphic rocks （95 90 Ma）. The data are interpreted as dating high-grade metamorphism during subduction burial of the sediments into hot mantle lithosphere, and rapid cooling during their subsequent exhumation. A few discordant zircon ages, interpreted as late Neoproterozoic and younger, represent minor detrital input from the continent. No evidence is found in favour of the existence of older Arabian continental crust within the metamorphic rocks of the UAE.

Phenotypic and genetic parameter estimation of juvenile growth and bottom color traits in half-smooth tongue sole,Cynoglossus semilaevis

Half-smooth tongue sole（Cynoglossus semilaevis） is a commercially important fish species. There were no reports about accurate genetic parameters of growth traits in C. semilaevis, despite efforts aimed at the culture of this species. Therefore, the main goal of this study was to estimate the parameters for the early growth traits（total length, body depth and body weight） of C. semilaevis based on the data obtained from 80 families. The trait of fish with pure white bottom or not（named as the trait of bottom color） was also analyzed. All the heritabilities estimated for the traits were medium, which varied from 0.210 to 0.362. Genetic correlations among growth traits were highly positive, varying from 0.913 to 0.959, indicating that selection of one of the three traits would result in correlated increase in the others. Also, genetic correlations between bottom color and growth traits were positive and varying from 0.241 to 0.353, suggesting that selection for the bottom color can be utilized to enhance the selection of growth traits. In addition, fish from the top performing 16 full-sib families were selected as broodstock, based on the above analysis and the comparison of family breeding value. This research provides an important basic material to implement selective breeding in C. semilaevis.

Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole(Cynoglossus semilaevis)

An astroglial cell line was established from the brain of half smooth tongue sole （Cynoglossus semilaevis） and was designated as CSAC. CSAC shows the morphological homogeneity of epithelial cells. The cell identity was tested by the presence of glial fibrillary acidic protein （GFAP）, which was revealed by RT-PCR and immunofluorescence. The cell line was optimally maintained at 24 ℃ in minimum essential medium supplemented with HEPES, antibiotics, 20% fetal bovine serum, 2- Mercaptoethanol （2-Me） and basic fibroblast growth factor. Chromosome analysis revealed that the CSAC cells maintained a normal diploid chromosome number （2n=42）. The fluorescent signals were observed in CSAC after the cells were transfected with green fluorescent protein （GFP） reporter plasmids. The CSAC cell line may serve as a valuable tool for studies on the potential functions of fish astroglial cells.

Thermal evolution of an ancient subduction interface revealed by Lue Hf garnet geochronology, Halilba■? Complex(Anatolia)

The thermal structure of subduction zones exerts a major influence on deep-seated mechanical and chemical processes controlling arc magmatism, seismicity, and global element cycles. Accretionary complexes exposed inland may comprise tectonic blocks with contrasting pressureetemperature(Pe T)histories, making it possible to investigate the dynamics and thermal evolution of former subduction interfaces. With this aim, we present new Lue Hf geochronological results for mafic rocks of the Halilbag?Complex(Anatolia) that evolved along different thermal gradients. Samples include a lawsoniteeepidote blueschist, a lawsoniteeepidote eclogite, and an epidote eclogite(all with counter-clockwise Pe T paths),a prograde lawsonite blueschist with a "hairpin"-type Pe T path, and a garnet amphibolite from the overlying sub-ophiolitic metamorphic sole. Equilibrium phase diagrams suggest that the garnet amphibolite formed at w0.6 -0.7 GPa and 800 -850℃, whereas the prograde lawsonite blueschist records burial from 2.1 GPa and 420℃ to 2.6 GPa and 520℃. Well-defined Lue Hf isochrons were obtained for the epidote eclogite(92.38 ± 0.22 Ma) and the lawsoniteeepidote blueschist(90.19 ± 0.54 Ma),suggesting rapid garnet growth. The lawsoniteeepidote eclogite(87.30 ± 0.39 Ma) and the prograde lawsonite blueschist(ca. 86 Ma) are younger, whereas the garnet amphibolite(104.5 ± 3.5 Ma) is older.Our data reveal a consistent trend of progressively decreasing geothermal gradient from granulite-facies conditions at ~104 Ma to the epidote-eclogite facies around 92 Ma, and the lawsonite blueschist-facies between 90 Ma and 86 Ma. Three Lue Hf garnet dates(between 92 Ma and 87 Ma) weighted toward the growth of post-peak rims(as indicated by Lu distribution in garnet) suggest that the HP/LT rocks were exhumed continuously and not episodically. We infer that HP/LT metamorphic rocks within the Halilbag?Complex were subjected to continuous return flow, with "warm" rocks being exhumed during the tectonic burial of "cold" ones. Our results, combined with regional geological constraints, allow us to speculate that subduction started at a transform fault near a mid-oceanic spreading centre. Following its formation, this ancient subduction interface evolved thermally over more than 15 Myr, most likely as a result of heat dissipation rather than crustal underplating.

Adding body load modifies the vibratory sensation of the foot sole and affects the postural control

Background: Heavy backpacks are often used by soldiers and firefighters. Weight carrying could reduce the speed and efficiency in task completion by altering the foot sole sensitivity and postural control.Methods: In fifteen healthy subjects, we measured the changes in sensitivity to vibrations applied to the foot sole when standing upright or walking after load carrying(30% body weight). The participants were asked to judge different vibration amplitudes applied on the 2 nd or 5 th metatarsal head and the heel at two frequencies(25 and 150 Hz) to determine the vibration threshold and the global perceptual representation(Ψ)of the vibration amplitude(Φ)given by the Stevens power function(Ψ=k×Φ~n). Any increase in negative k value indicated a reduction in sensitivity to the lowest loads. Pedobarographic measurements, with computation of the center of pressure(COP) and its deviations, were performed during weight carrying.Results: The 25-Hz vibration threshold significantly increased after weight carrying when standing upright or walking.After standing with the added loads, the absolute negative k value increased for the 25 Hz frequency. After walking with the added loads, the k coefficient increased for the two vibration frequencies. Weight carrying significantly increased both the CoP surface and CoP lateral deviation.Conclusions: Our data show that weight carrying reduces the sensory pathways from the foot sole and accentuates the center of pressure deviations.

B7-H4 as a protective shield for pancreatic islet beta cells

Auto- and alloreactive T cells are major culprits that damage β-cells in type 1 diabetes(T1D) and islet transplantation. Current immunosuppressive drugs can alleviate immune-mediated attacks on islets. T cell co-stimulation blockade has shown great promise in autoimmunity and transplantation as it solely targets activated T cells, and therefore avoids toxicity of current immunosuppressive drugs. An attractive approach is offered by the newly-identified negative T cell cosignaling molecule B7-H4 which is expressed in normal human islets, and its expression co-localizes with insulin. A concomitant decrease in B7-H4/insulin colocalization is observed in human type 1 diabetic islets. B7-H4 may play protective roles in the pancreatic islets, preserving their function and survival. In this review we outline the protective effect of B7-H4 in the contexts of T1 D, islet cell transplantation, and potentially type 2 diabetes. Current evidence offers encouraging data regarding the role of B7-H4 in reversal of autoimmune diabetes and donor-specific islet allograft tolerance. Additionally, unique expression of B7-H4 may serve as a potential biomarker for the development of T1 D. Futurestudies should continue to focus on the islet-specific effects of B7-H4 with emphasis on mechanistic pathways in order to promote B7-H4 as a potential therapy and cure for T1 D.

Half Smooth Tongue Sole(Cynoglossus semilaevis) Under Low Salinity Stress Can Change Hepatic igf2 Expression Through DNA Methylation

Salinity is a crucial environmental stress that severely affects fish growth and survival.Under environmental stress,DNA methylation plays an important role in gene expression and genome function.To better understand the epigenetic regulation mechanism of igf2 under low salinity stress,we analyzed the DNA methylation at 5’UTR,exon1,intron1,and exon2,and analyzed the relationship of DNA methylation with mRNA abundance as well as the special single CpG sites methylation patterns of igf2 in the liver of half smooth tongue sole under low salinity(15)for 0,7,and 60 d.When exposed to low salinity,DNA methylation at 5’UTR and exon2 remained stable,while it was up-regulated firstly and then down-regulated at exon1 and intron1.Some single CpG sites of igf2 associated with low salinity,and most of these sites with significantly changed methylation levels(P<0.05)are located in intron1 area.The discrepant variation of single CpG sites methylation levels and igf2 expression further revealed that females and males showed different response to low salinity.Remarkably,the 38-CpG site of intron1 servers as a sexual marker.Additionally,our integrative analysis demonstrated that regional DNA of igf2 methylation had highly complex interplay on gene expression.The single CpG sites in intron1 were indispensable epigenetic markers under external environmental stress.Above all,to resist the low salinity stress,half smooth tongue sole liver can regulate the expression of igf2 through methylation of CpG sites in intron1.

Comparative evaluation of modified neem leaf, neem leaf and woodash extracts on soil fertility improvement, growth and yields of maize (<i>Zea mays</i>L.) and watermelon (<i>Citrullus lanatus</i>) (Sole and Intercrop)

Two field experiments were carried out at Akure (7oN, 5o101E) in the rainforest zone of Nigeria in 2006 and 2007 to determine the effectiveness of neem leaf, woodash and modified neem leaf extracts as fertilizer sources in improving soil fertility, growth and yield of maize (Zea mays L) and watermelon (Citrulus lanatus) sole and intercrop. There were six treatments namely, poultry manure, neem leaf extract (sole), woodash extract, modified neem leaf (neem leaf + woodash), NPK 15-15-15 and a control (no fertilizer nor extract), replicated three times and arranged in a randomized complete block design (RCB). The extracts (neem leaf, wood ash and modified neem leaf) were applied at 1200 litres per hectare each, NPK 15-15-15 at 300 kg/ha and poultry was applied at 6t/ha. The results showed that there were significant increases (P 2O), K, Ca, Mg, Na, O.M, P and N compared to NPK 15-15-15 and neem leaf extract. For instance, modified neem leaf extract increased soil pH (H2O), K, Ca, Mg, Na, O.M, P and N by 12.4%, 32.8%, 25%, 23.7%, 19.32%, 17.24% and 20% respectively compared to neem leaf extract under intercrop plot. The high soil K/Ca, K/Mg and P/Mg ratios in the NPK 15-15-15 fertilizer treatment led to an imbalance in the supply of P, K, Ca and Mg nutrients to maize and watermelon crops. The least values for growth, yield and soil parameters were recorded under the control treatment. In these experiments, modified neem leaf extract (woodash + neem leaf extracts) applied at 1200 litres/ha was the most effective in improving soil fertility, growth and yield of maize and watermelon (sole and intercrop) and could substitute for 6 tons per hectare of poultry manure and 300kg/ha of NPK 15-15-15 fertilizer.

Identification and Characterisation of a Bacterial Isolate Capable of Growth on Trichloroethylene as the Sole Carbon Source

The aim of this research work was to isolate microbes from soil, to investigate their potential use as effective bioremediation tools for trichloroethylene—a potent environmental pollutant. The isolate showing good growth in presence of TCE was named PM102. Microbiological characterisation of the PM102 isolate showed that it was a gram negative rod. Detailed structure was revealed by scanning electron microscopy. pH and temperature optima, salt tolerance and optimum TCE concentration for growth of PM102 were determined. The ability of this bacterium to degrade TCE was studied in acidic and neutral pH by biochemical test and chloride release. Five TCE inducible bands were detected in the protein profile of the isolate as studied by SDS PAGE. A major TCE inducible band of 51.61 kDa was excised from the gel and injected into rabbit to raise specific antibody. The bacterium was identified as Stenotrophomonas maltophilia PM102 by 16S rDNA amplification and sequencing. The 16S rRNA gene sequence has been deposited in the NCBI GenBank with accession number JQ797560. This genus has not been described previously as being capable of TCE degradation. We report for the first time a Stenotrophomonas sp. that grows on TCE as the sole carbon source.

Isolation and Identification of Photobacterium damselae subsp.damselae(PDD) from Tongue Sole

In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tissues of sick tongue sole aseptically collected.The isolate was identified as Photobacterium damselae subsp.damsela（PDD） by isolation culture,Gram staining,physiological identification,morohological observation,biochemical identification and 16S rDNA sequence analysis.The results showed that the isolate shared 99.6% homology with the reference strain in GenBank.The animal regression test displayed that the isolate had very strong pathogenicity to tongue sole.The LD（50） was 3.1 × 10~4 CFU/mL,and it showed pathogenicity to mammals.The antimicrobial susceptibility test showed the isolate was highly sensitive to nrofloxacin,Norfloxacin,Ciprofloxacin,Mequindox;moderately sensitive to Cefradine,Doxycycline;and insensitive to Gentamicin,Ceftriaxone,Tilmicosin,etc..

Varietal Performance of Turmeric under Mango Based Agroforestry System

An experiment was carried out at the Agroforestry and Environment Research Farm, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh, during April, 2014 to January 2015. The experiment was laid out in two factors Randomized Complete Block Design (RCBD) with three replications. Among the two factors, one factor like A was two production systems: S1 = Mango + Turmeric and S2 = Turmeric (sole crop);another factor like B was three turmeric variety: V1 = BARI Holud-1, V2 = BARI Holud-2 and V3 = BARI Holud-3. So, the treatment combinations were: S1V1 = Mango + BARI Holud-1, S1V2 = Mango + BARI Holud-2, S1V3 = Mango + BARI Holud-3, S2V1 = sole cropping of BARI Holud-1, S2V2 = sole cropping of BARI Holud-2 and S2V3 = sole cropping of BARI Holud-3. The result of the experiment revealed that interaction effect of production systems and variety was found significant on plant height, number of leaf per plant, length of leaf blade, breadth of leaf blade, number of finger per rhizome, fresh and dry yield per hectare. The higher fresh yield of turmeric (34.75 t/ha) and dry yield (6.10 t/ha) was found from S1V1 treatment (Mango + BARI Holud-1). Whereas the lowest fresh yield (33.41 t/ha) and dry yield (4.93 t/ha) was found from S2V2 treatment (sole cropping of BARI Holud-2). However, the suitability of the cultivation of different turmeric variety under mango based agroforestry systems may be ranked as S1V1 > S2V1 > S1V3 > S2V3 > S1V2 > S2V2. Finally it may be concluded that, BARI Holud-1 would be the best variety to be grown under mango based agroforestry.

Productivity status of traditional agrisilviculture system on northern and southern aspects in mid-hill situation of Garhwal Himalaya,India

The productivity of traditional agrisilviculture system(agricultural crops + trees) was investigated in the northern and southern aspects of mid-hill situation in Garhwal Himalaya, Uttarakhand, India during the 2004?2006.A total of 19 tree species were studied in both northern and southern aspects, out of which 17 tree species were selected in northern aspect and 12 tree species in southern aspect for phytosociological characteristic analysis of trees in agrisilvicultural system.The most dominant tree species are Grewia optiva, Celtis australis and Melia azedarach and successively grown under traditional agrisilviculture system.The results show that the annual productivity of all tree species was 3 775 kg·ha-1·a-1 in northern aspect(site-N) and 3 101 kg·ha-1·a-1 in southern aspect(site-S).G.optiva had the highest productivity in both site-N and site-S among the tree species, followed by M.azedarach, Quercus leucotrichophora and C.australis.The dominant agricultural crops were Eleusine coracana in summer cereals, Phaseolus vulgaris in summer pulses-oilseeds and Triticum aestivum in the winter season in the area.The average biological productivity of agricultural crops in northern aspect was about 16% higher than that in southern aspect under traditional agrisilviculture system.The sole agricultural crop productivity(without trees) in northern aspect was also higher than that in southern aspect.An obvious difference in annual productivity of trees and agriculture crops was observed between northern aspect and southern aspect.The overall productivity in traditional agrisilviculture system(crop + tree) was 24%(in northern aspect) and 21%(in southern aspect) higher than that in sole cropping system.

印度的加瓦尔喜马拉雅山丘中部南北坡的传统农林复合系统中生产力状况

The productivity of traditional agrisilviculture system （agricultural crops ＋ trees） was investigated in the northern and south- ern aspects of mid-hill situation in Garhwal Himalaya, Uttarakhand, India during the 2004-2006. A total of 19 tree species were studied in both northern and southern aspects, out of which 17 tree species were selected in northern aspect and 12 tree species in southern aspect for phytosociological characteristic analysis of trees in agrisilvicultural system. The most dominant tree species are Grewia optiva, Celtis australis and Melia azedarach and successively grown under traditional agrisilviculture system. The results show that the annual produc- tivity of all tree species was 3 775 kg.ha-1.a^-1 in northern aspect （site-N） and 3 10! kg.ha^-1.a^-1 in southern aspect （site-S）. G. optiva had the highest productivity in both site-N and site-S among the tree species, followed by M. azedarach, Quercus leucotrichophora and C. australis. The dominant agricultural crops were Eleusine coracana in summer cereals, Phaseolus vulgaris in summer pulses-oilseeds and Triticum aestivum in the winter season in the area. The average biological productivity of agricultural crops in northern aspect was about 16% higher than that in southern aspect under traditional agrisilviculture system. The sole agricultural crop productivity （without trees） in northern aspect was also higher than that in southern aspect. An obvious difference in annual productivity of trees and agriculture crops was observed between northern aspect and southern aspect. The overall productivity in traditional agrisilviculture system （crop ＋ tree） was 24% （in northern aspect） and 21% （in southern aspect） higher than that in sole cropping system.