Correlation of matrix metalloproteinase－2, －9, tissue inhibitor－1 of matrix metalloproteinase and CD44 variant 6 in head and neck cancer metastasis

This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase 2, 9 (MMP 2, MMP 9), tissue inhibitor 1 of matrix metalloproteinase (TIMP 1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP 2, MMP 9, TIMP 1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship ( P <0.05) between the expression of MMP 2 and MMP 9, TIMP 1 and CD44v6, HER2/neu and MMP 9, MMP 2 and p53. Up regulation of MMP 2 was accompanied by advanced T stage ( P <0.01) . There was also a trend of MMP 2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence( P <0.05). The expression of TIMP 1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non keratinizing SCC than that in basaloid SCC( P <0.05). These findings suggested that MMP 2 and MMP 9, HER2/neu and MMP 9, MMP 2 and p53 had a coordinate function in aggression of tumor; that MMP 2 had a more important function than MMP 9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC.

A STUDY OF ADHESIVE MOLECULE CD_(44S)CD_(44V) VARIANT EXPRESSION IN GASTRIC CARCINOMA WITH RT-PCR

Objective To investigate the correlation between transcript CD 44S , CD 44V and gastric carcinoma invasion metastasis, and to know what role the CD 44 gene's abnormal activity plays in the genesis and development of gastric cancer. Methods RT-PCR was used to detect mRNA of CD 44V and CD 44S in 10 gastric normal mucosa,8 gastric precancerous mucosa,10 tissue adjacent to cancer,40 gastric carcinoma (including metastatic focus) with different pathological features.Results All gastric carcinomas and non-cancerous tissues expressed CD 44S ,but CD 44V expression rate were 0%, 12.5%,20% and 72.5% ,respectively, in normal gastric mucosa, precancerous tissue,tissue adjacent to cancer,carcinoma. Cancer invasion limited to submucosal layer showed lower CD 44V expression rate than invasion extended beyond the muscle layer (P<0.05). Samples with lymph node metastasis showed higher CD 44V expression rate than samples without lymph node metastasis,meanwhile, there was significant correlation between metastasis limited to N1 and beyond N1 (P<0.05). There was no relationship between CD 44V expression and Lauren's type, TNM stage as well (P>0.05). Conclusion There is a close relation between CD 44V and genesis and development of gastric cancer. CD 44 gene variant splicing is an early genetic affair in gastric cancer.It could be a diagnostic tool to screen high-risk people for gastric carcinoma. CD 44V can be regard as a parameter in evaluating the potential of cancer cell invasion and metastasis ,as well as prognosis.

Bioinformatics research of CD44 and epithelial cell adhesion molecule related genes and pathways in colorectal cancer

Objective To investigate genes and involved biological processes closely associated with stem cell markers of colorectal cancer-epithelial cell adhesion molecule(EpCAM)+and CD44+.Methods By the bioinformatics method,with microarray data of colorectal cancer from gene expression omnibus(GEO)database and R2 platform,the genes significantly related with CD44 and Ep-

粘附分子CD_(44)在乳腺癌中的表达及其意义

目的 :探讨粘附分子CD4 4 在乳腺癌中的表达及其临床意义。方法 :采用逆转录聚合酶链反应(RT PCR)方法对 30例乳腺癌及 10例乳腺良性肿瘤组织中的CD4 4 基因的表达进行检测。结果 :①CD4 4 V6在乳腺癌中的表达显著性高于乳腺良性肿瘤 ;②乳腺癌组织中CD4 4 V6表达率明显高于CD4 4 S及CD4 4 V3;③Ⅲ、Ⅳ期乳腺癌CD4 4 V6表达显著高于Ⅰ、Ⅱ期乳腺癌 ;④有淋巴结转移的乳腺癌CD4 4 V6的表达明显高于无淋巴结转移者。结论 :CD4 4 V6在乳腺癌中的表达与乳腺癌的发生发展、浸润转移和预后密切相关 。

大肠癌患者外周血细胞CD_(44)s和CD_(44)V_5及CD_(44)V_6表达的研究

目的 :探讨大肠癌患者外周血细胞CD44s、CD44V5和CD44V6的表达与肿瘤转移和预后的关系。方法 :采用流式细胞术对 6 2例大肠癌患者外周血细胞CD44s、CD44V5和CD44V6的表达率进行检测 ,并分析他们之间的相关性。结果 :大肠癌患者外周血细胞的CD44V5+ 细胞和CD44V6+ 细胞检出率均显著高于正常对照组 ,P <0 0 1;且转移者显著高于未转移者 ,P <0 0 1;而CD44s+ 细胞检出率却显著低于正常对照组 ,P <0 0 5 ;且伴转移者显著低于未转移者 ,P <0 0 1;肿瘤患者外周血细胞CD44V5+ 和CD44V6+ 细胞表达率与CD44s+ 细胞检出率呈显著的负相关 ,r1=0 84 ,P1<0 0 5和r2 =- 0 .87,P2 <0 .0 1。结论 :CD44V5及CD44V6的表达与肿瘤转移密切相关 ,检测大肠癌患者外周血中的CD44s、CD44V及CD44V6的表达可为肿瘤的发生、发展及预后判断提供依据。

CD44v6在卵巢上皮性癌中的表达及意义

目的 探讨CD44v6在卵巢癌中的表达情况及其与卵巢癌临床病理特征之间的关系,进一步阐明CD44v6在卵巢癌侵袭和转移过程中的作用.方法 通过免疫组化SP法检测在CD44v6正常卵巢组织、卵巢良性、交界性、恶性肿瘤组织中的表达.结果 CD44v6在卵巢癌中的表达与肿瘤的分化程度、肿瘤的临床分期及有无淋巴结转移有关,差异有统计学意义,而与上皮性肿瘤中的不同组织学类型无关.结论 CD44v6在正常卵巢组织中不表达,在卵巢上皮性癌中呈高表达,并与临床分期、分级、淋巴结转移有关,提示CD44v6的表达可能与卵巢上皮性癌的发生、发展有关。

CD_(44)表达与乳腺癌侵袭转移相关性的研究

目的 :研究乳腺癌组织中粘附分子 CD44 的表达及其对肿瘤发生、发展和转移的影响。方法 :应用免疫组织化学方法 ,检测 30例乳腺癌和 10例乳腺良性肿瘤组织中 CD44 S、CD44 V3 及 CD44 V6 表达情况。结果 :CD44 S、CD44 V3 表达在乳腺癌和良性乳腺肿瘤组织中无显著差异 ,CD44 V6 表达在乳腺癌组织明显高于良性组织 ;CD44 v6 表达与临床分期相关 ,与病理类型无关 ;有淋巴结转移的乳腺癌组织中 ,CD44 V6 表达明显高于无淋巴结转移者。结论 :CD44 (尤其是 CD44 V6 )的异常表达与乳腺癌的发生发展、浸润转移密切相关 ;对评价乳腺癌恶性程度。