Changes of soluble intercellular adhesion molecule-1 in the serum from patients with acute cerebral infarction

objective: To observe the changes of soluble intercellular adhesion molecule-1(sICAM-1) in the serum of patients with acute cerebral infarctlon (ACI) and their clinical significance. Methods: The concen-tration of sICAM-1 in the serum of 91 patients with ACI was determined with ELISA and then the results were compared wlth those of 43 patients with cerebral hemorrhage and 30 healthy individuals. Results: In the 24th hour after infarction. the concentration of sICAMu-1 in the serum was significantly higher in patients with ACI than in patients with cerebral hemorrhage and normal controls (P< 0. 01). In the patients with ACI, the concentration exhibited an decreasing tendency in the period from the 24th hour to the 14th day andwas correlated with the focal size of cerebral infarction. During the first 14 days after infarction, the concen-tration was significantly higher in the patients with the complication of infection than in those without. Con-clusion: sICAM-1 is closely correlated with clinical manifestation of ACI.

Relationship of plasma homocysteine, soluble intercellular adhesion molecule-1, high mobility group box 1 protein with carotid intima-media thickness in elderly patients with type 2 diabetes mellitus

Objective:To explore the relationship of plasma homocysteine(Hcy),soluble intercellular adhesion molecule-1(sICAM-1)and high mobility group box 1 protein(HMGB1)with carotid intima-media thickness(c-IMT)in elderly patients with type 2 diabetes mellitus.Methods:A total of 100 elderly patients who were diagnosed as type 2 diabetes mellitus in Baogang Hospital of Inner Mongolia from June 2017 to May 2020 were chosen as research objects.According to c-IMT,they were divided into the normal group(n=35),the mild to moderate group(n=41)and the severe group(n=24).The expression levels of plasma Hcy,sICAM-1 and HMGB1 were compared between groups respectively.Pearson’s correlation coefficient was used to analyze the relationship of plasma Hcy,sICAM-1,HMGB1 with c-IMT.Results:The comparison in plasma Hcy,sICAM-1,HMGB1 and c-IMT among the three groups of patients was of statistical significance(p<.05).The results of correlation analysis showed that the expression levels of plasma Hcy,sICAM-1 and HMGB1 were positively correlated with c-IMT in elderly patients with type 2 diabetes mellitus(r=.627,.598,.614;p<.05).Conclusions:The expression levels of plasma Hcy,sICAM-1 and HMGB1 are abnormally increased in elderly patients with type 2 diabetes mellitus,and related to c-IMT,which can provide a strong evidence for clinical diagnosis and treatment by detecting their levels in clinical practice.

Increased Risk of Neonatal Pneumonia in Pregnant Women with Atypical Pre-Labor Rupture of Membrane Assessed at Pregnancy Week 39

Purpose: Neonatal pneumonia is a major newborn disease with a high morbidity rate. We aimed to evaluate whether atypical prelabor rupture of membranes (PROM) is a high-risk factor for causing neonatal pneumonia in a prospective real-world study. Patients and Methods: A total of 250 pregnant women at pregnancy week 39 were non-selectively recruited. All were examined by PROM and neonatal pneumonia related clinical, bedside and lab tests, including body temperature, blood pressure, increased vagina discharge, posterior vault pooling, abdominal tenderness, WBC count, nitrazine test, amniotic fluid index, Leakection (a sICAM-1 based lateral flow immunoassay) and vagina streptococcus examinations. Increased vagina discharge with a Leakection positivity was adopted as a working criterium for identifying atypical PROM. Neonatal pneumonia was diagnosed based on the clinical presentation and lab tests. Results: Twenty cases of neonatal pneumonia (8.0%) were diagnosed after the deliveries of the 250 pregnant women. In these neonatal pneumonia cases, 12 (16.7%) occurred in 72 deliveries with atypical PROM, 2 (16.7%) in 12 deliveries with typical PROM, and 6 (3.6%) in 166 deliveries with non-PROM. Conclusion: In this real-world study, we find that a systematic screening at pregnancy week 39 was very meaningful in revealing atypical PROM. Moreover, atypical PROM is a major risk factor for neonatal pneumonia. Therefore, an early diagnosis and intervention on atypical PROM could potentially reduce the occurrence of neonatal pneumonia.

吸入白细胞介素-5对哮喘患者血清可溶性粘附分子-1水平的影响

目的：观察白细胞介素－５（ＩＬ－５）经雾化吸入后对过敏性哮喘患者和正常人血清中可溶性粘附分子－１（ｓＩＣＡＭ－１）水平变化的影响。方法：于ＩＬ－５吸入前、吸入后２、２４、４８及７２ｈ分别收集８例哮喘患者和６名正常对照者的血清，并以酶联免疫吸附法测定其中ｓＩＣＡＭ－１水平。结果：ＩＬ－５对正常人血清中的ｓＩＣＡＭ－１水平均无影响。哮喘患者吸入ＩＬ－５后２ｈｓＩＣＡＭ－１即明显增高，４８ｈ后达最大值，而这种高水平的ｓＩＣＡＭ－１至少可以维持７２ｈ。结论：ＩＬ－５可以明显提高哮喘患者而不是正常人的ｓＩＣＡＭ－１水平；

冠心病伴牙周炎患者牙周治疗前后血清sICAM-1的变化

目的观察冠心病伴牙周炎患者在牙周治疗前后血清slCAM．1的变化。方法选取冠心病（稳定期）伴牙周炎患者55例，分为牙周治疗^山内科治疗组29例（实验组）和心内科治疗组26例（对照组）。对实验组患者行牙周治疗，采集2组治疗前和治疗后1个月牙周临床参数和血清样本。应用酶联免疫吸附法测定血清slCAM-1浓度。结果实验组和时照组患者}台疗前牙周临床参数和血清slCAM。1浓度无统计学差异（P〉0．05）；实验组患者经过牙周治疗后的牙周临床参数和血清slCAM-1浓度较治疗前均明显降低，差异有统计学意义（P〈O．05）。而对照组患者各项指标无明显变化。结论冠心病伴牙周炎患者通过牙周治疗可以降低血清中slCAM-1浓度，改善血管内皮细胞功能，利于冠心病的预防和治疗，降低其再发风险。

Change of hs-CRP,sVCAM-1,NT-proBNP levels in patients with pregnancy-induced hypertension after therapy with magnesium sulfate and nifudipine

Objective:To investigate the change of the hs-CRP,sVC AM-1,NT-proBNP levels of the patients with pregnancy-induced hypertension(PIH) syndrome.Methods:A total of 200 patients with PIH were divided into mild,moderate and severe group,and 50 healthy pregnancy patients served as the control group.The serum sVCAM-1 levels were detected by enzyme-linked immunosorbent assay,hs-CRP were detected by immunity transmission turbidity,and NT-proBNP levels were determined by the colloidal gold method.Patients were treated with magnesium sulfate and nifudipine and the contrastive analysis was performed before and after treatment.And the pathological changes in placental of PIH patients were delected by hematoxylin-eosin staining at the same time.Results:The hs-CRP,sVCAM-l,NT-proBNP levels of patients in the mild, moderate and severe PHI group were significantly higher than that in the control group(P<0.05). The hs-CKP,sVCAM-l,NT-proBNP levels in the severe group were significantly higher than the mild group and the moderate group,the difference was statistically significant(P<0.05).The hsCRP,sVCAM-l,NT-proBNP of the moderate group were significantly higher than the mild group(P<0.05).There was a positive correlation between hs-CRP,sVCAM-1,NT-proBNP expression levels and the degree of the PIH.The expression of hs-CRP,sVCAM-1,NT-proBNP levels of the moderate and the severe group were significantly decreased(P<0.05).The number of placental villi and interstitial blood vessel in the moderate and severe PIH group were significantly less than the control group(P<0.05).Conclusions:The increased levels of serum hs-CRP,sVCAM-1, NT-proBNP may be involved in the process of vascular endothelial cell injury of the PIH,and the hs-CRP,sVCAM-1,NT-proBNP can be used as the auxiliary index for diagnosis of PIH and determination of PIH severity.

磷酸二酯酶4抑制剂Hemay005对角质形成细胞炎性因子产生的影响

目的为了探讨磷酸二酯酶4抑制剂Hemay005对体外培养的角质形成细胞株HaCaT细胞炎性因子产生的影响。方法分别用重组人肿瘤坏死因子-α(rhTNF-α)、重组人干扰素-γ(rhIFN-γ)和312 nm窄波UVB诱导,采用MTT法检测Hemay005对HaCaT细胞增殖的影响,用ELISA法检测其对HaCaT细胞白细胞介素(IL)-8、sICAM-1和肿瘤坏死因子(TNF)-α产生的影响。结果 Hemay005对HaCaT细胞增殖及25μg/L rhTNF-α诱导的IL-8产生无明显影响,但能剂量依赖性地抑制1 000 U/mL rhIFN-γ诱导的HaCaT细胞产生sICAM-1和124.2 mJ/cm2窄波UVB照射引起的HaCaT细胞产生TNF-α。结论 Hemay005可通过抑制角质形成细胞炎症因子的表达发挥抗炎作用。

阿托伐他汀通过过氧化体增殖物激活型受体γ促进一氧化氮生成抑制炎性因子产生

目的观察阿托伐他汀是否能通过激活人脐静脉内皮细胞的过氧化体增殖物激活型受体γ从而改善血管内皮功能。方法将体外培养的人脐静脉内皮细胞的实验分为两部分,实验一分组:①对照组;②脂多糖组(1.0 mg/L);③阿托伐他汀1.0 mmol/L组;④脂多糖+阿托伐他汀1.0 mmol/L组;⑤脂多糖+阿托伐他汀5.0mmol/L组,经孵育24 h后,收集细胞和培养上清液,用RT-PCR方法测定不同浓度阿托伐他汀对人脐静脉内皮细胞的过氧化体增殖物激活型受体γ表达的影响,并用硝酸还原酶法测定不同浓度阿托伐他汀干预对脂多糖诱导后细胞培养上清液中的一氧化氮生成的影响,ELISA方法测定细胞培养上清液中人可溶性细胞间黏附分子含量的影响。实验二分组:①对照组;②阿托伐他汀5.0 mmol/L组;③0.2 mmol/L GW 9662组;④脂多糖+阿托伐他汀5.0mmol/L组;⑤GW 9662+脂多糖+阿托伐他汀5.0 mmol/L组,观察过氧化体增殖物激活型受体γ特异性阻断剂GW 9662对阿托伐他汀与脂多糖共同作用后人脐静脉内皮细胞的过氧化体增殖物激活型受体γ表达及培养上清液中一氧化氮、人可溶性细胞间黏附分子1含量变化的影响。结果不同浓度阿托伐他汀可上调人脐静脉内皮细胞的过氧化体增殖物激活型受体γ表达,且随着药物浓度的增加其上调受体表达的作用增强。不同浓度阿托伐他汀可干预脂多糖诱导的人脐静脉内皮细胞液中一氧化氮生成减少及人可溶性细胞间黏附分子1含量的增加,且随着药物浓度的增加上述作用增强。过氧化体增殖物激活型受体γ特异性阻断剂GW 9662可部分阻断阿托伐他汀上述作用。结论阿托伐他汀可能部分通过激活人脐静脉内皮细胞的过氧体增殖物激活型受体γ受体,促进一氧化氮生成,抑制炎性因子的产生,改善血管内皮功能。

Early detection of hepatocellular carcinoma co-occurring with hepatitis C virus infection: A mathematical model

AIM: To develop a mathematical model for the early detection of hepatocellular carcinoma(HCC) with a panel of serum proteins in combination with α-fetoprotein(AFP).METHODS: Serum levels of interleukin(IL)-8, soluble intercellular adhesion molecule-1(s ICAM-1), soluble tumor necrosis factor receptor Ⅱ(s TNF-RⅡ), proteasome, and β-catenin were measured in 479 subjects categorized into four groups:(1) HCC concurrent with hepatitis C virus(HCV) infection(n = 192);(2) HCV related liver cirrhosis(LC)(n = 96);(3) Chronic hepatitis C(CHC)(n = 96); and(4) Healthy controls(n = 95). The R package and different modules for binary and multi-class classifiers based on generalized linear models were used to model the data. Predictive power was used to evaluate the performance of the model. Receiver operating characteristic curve analysis over pairs of groups was used to identify the best cutoffs differentiating the different groups. RESULTS: We revealed mathematical models, based on a binary classifier, made up of a unique panel of serum proteins that improved the individual performance of AFP in discriminating HCC patients from patients with chronic liver disease either with or without cirrhosis. We discriminated the HCC group from the cirrhotic liver group using a mathematical model(-11.3 + 7.38 × Prot + 0.00108 × s ICAM + 0.2574 ×β-catenin + 0.01597 × AFP) with a cutoff of 0.6552, which achieved 98.8% specificity and 89.1% sensitivity. For the discrimination of the HCC group from the CHC group, we used a mathematical model [-10.40 + 1.416 × proteasome + 0.002024 × IL + 0.004096 × s ICAM-1 +(4.251 × 10-4) × s TNF + 0.02567 ×β-catenin + 0.02442 × AFP] with a cutoff 0.744 and achieved 96.8% specificity and 89.7% sensitivity. Additionally, we derived an algorithm, based on a binary classifier, for resolving the multi-class classification problem by using three successive mathematical model predictions of liver disease status. CONCLUSION: Our proposed mathematical model may be a useful method for the early detection of different statuses of liver disease co-occurring with HCV infection.

Changes of serum levels of ICAM-1,IGF-1 and IL-8 in normal offspring with a family history of essential hypertension

Background Essential hypertension （EH） has become the most common chronic non-infectious epidemic and is one of the most common risk factors for the damage to heart, brain, kidney and other organs. The serum levels of ICAM-1, IGF-1 and IL-8 play important roles in the pathogenesis of EH. Methods In the medical check-up center of the Affiliated Hospital of Qingdao University Medical College, sixty normal offspring with a family history of EH were randomly recruited into two groups： 30 offspring with a father or mother suffering from EH as single-parent group, and 30 offspring with both parents suffering from EH as double-parent group, and another 30 normal offspring whose parents did not suffer from EH as control group. The serum levels of ICAM-1, IGF-1 and IL-8 were determined by enzyme-linked immunosorbent assay （ELISA）. Result The serum levels of ICAM-1, IGF-1 and IL-8 were significantly higher in both single-parent group and double-parent group than in the control group （P 〈 0.05）, and the serum levels of ICAM-1, IGF-1 and IL-8 were higher in the double-parent group than in the single-parent group （P 〈 0.05）. The serum levels of ICAM-1, IGF-1 and IL-8 were positively correlated with the severity of blood pressure elevation （r = 0.375, r = 0.465, r = 0.326, P 〈 0.05, P 〈 0.01, P 〈 0.05 respectively）. Conclusions Due to the influence of heredity, the serum inflammatory factor contents in normal offspring with EH family history may increase before blood pressure rise. Detection of serum inflammatory factors in healthy offspring with a family history of EH could predict occurrence of hypertension, and provide a more reliable basis for the primary prevention of hypertension.

Change of Inflammatory Factors in Patients with Acute Coronary Syndrome

Background： Acute coronary syndrome （ACS） is closely related to unstable plaques and secondary thrombosis. The inflammatory cells in plaques and their inflammatory products may be the cause for plaque instability and ruptures. The study aimed to disclose the changes of inflammatory factors including serum intracellular adhesion molecule-1（ICAM-1 ）, chitinase-3-like protein I （YKL-40）, and lipoprotein-associated phospholipase A2 （Lp-PLA2） in patients with ACS and its clinical significance. Methods： A total of 120 patients with coronary heart disease （CHD） were categorized into 2 groups： 69 with ACS and 51 with stable angina pectoris （SAP）： 20 patients with chest pain and normal angiography served as a control group. The 120 patients with CHD were categorized into single-vessel disease group, double-vessel disease group, and three-vessel disease group based on the number of coronary artery stenosis. The severity of coronary artery stenosis was quantified based on coronary angiography using Gensini score. They were further divided into mild CHD group with its Gensini score 〈26 （n = 36）, moderate CHD group with its Gensini score being 26-54 （n = 48） and severe CHD group with its Gensini score 〉54 （n = 36）. Serum levels of ICAM-1, YKL-40, and Lp-PLA2 of different groups were determined by enzyme-linked immunosorbent assay. Correlation between ICAM-1, YKL-40, Lp-PLA2, and Gensini score was analyzed. Results： The levels of serum inflammatory factors ICAM-1, YKL-40, and Lp-PLA2 were significantly higher in the ACS group than those in control group and SAP group （all P 〈 0.05）： and compared with control group, no significant difference was observed in terms of the serum ICAM-1, YKL-40, and Lp-PLA2 levels in the SAP group （P 〉 0.05）.The levels of serum ICAM-1, YKL-40, and Lp-PLA2 were not significantly different among control group, single-vessel disease group, double-vessel disease group, and three-vessel disease group （all P 〉 0.05）. The levels of serum ICAM-1, YKL-40, and Lp-PLA2 were not significantly different among control group, mild CHD group （Gensini score 〈26）, moderate CHD group （Gensini score 26-54）, and severe CHD group （Gensini score 〉54） （all P 〉 0.05）. Nonparametric Spearman correlation analysis showed that the levels of serum ICAM-1, YKL-40, and Lp-PLA2 were not correlated with the Gensini score in CHD patients （r=0.093, r=-0.149, and r= -0.085, all P 〉 0.05; respectively）. Conclusions： The serum levels of ICAM-1, YKL-40, and Lp-PLA2 were correlated with different clinical types of CHD, but not well correlated the severity and extent of artery stenosis, suggesting that ICAM-1, YKL-40, and Lp-PLA2 rnight be involved in occurrence of instability of atherosclerotic plaque, and might reflect the severity of CHD mostly through reflecting the plaque stability.

Relationship between Two Blood Stasis Syndromes and Inflammatory Factors in Patients with Acute Coronary Syndrome

Objective： To investigate the relationship between inflammatory factors and two Chinese medicine（CM） syndrome types of qi stagnation and blood stasis（QSBS） and qi deficiency and blood stasis（QDBS） in patients with acute coronary syndrome（ACS）. Methods： Sixty subjects with ACS, whose pathogenesis changes belongs to qi disturbance blood stasis syndrome, were divided into 2 groups： 30 in the QSBS group and 30 in the QDBS group. The comparative analysis on them was carried out through comparing general information, coronary angiography and inflammatory factors including intracellular adhesion molecule-1（ICAM-1）, chitinase-3-like protein 1（YKL-40） and lipoprotein-associated phospholipase A2（Lp-PLA2）. Results： Compared with the QSBS group, Lp-PLA2 and YKL-40 levels in the QDBS group showed no-significant difference（P〉0.05）; ICAM-1 was significantly higher in the QDBS group than in the QSBS group in the pathological processes of qi disturbance and blood stasis syndrome of ACS（P〈0.05）. Conclusion： Inflammatory factor ICAM-1 may be an objective basis for syndrome typing of QSBS and QDBS, which provides a research direction for standardization research of CM syndrome types.