Eukaryotic expression, purification and activity characterization of human soluble DSG2 extracellular domain protein

Objective:To construct a secretory eukaryotic expression vector of DSG2 fused with the Fc region of the human IgG,to validate its expression in 293T cells,and to purify the secretory protein with biological activity.Methods:The DSG2 extracellular domain fragment gene(DSG2ex),was amplified by PCR,and was inserted into the eukaryotic expression plasmid pCMV3-IgG1 to construct the recombinant eukaryotic expression plasmid-pCMV3-DSG2ex-IgG1.The successfully constructed eukaryotic expression plasmid was transfected into 293T cells to express and secrete DSG2 extracellular domain protein.The targeted protein was purified from the cell culture supernatant by Protein A affinity chromatography and confirmed by Western Blotting and ELISA.Results:The pCMV3-DSG2ex-IgG1 eukaryotic expression plasmid was successfully constructed.The highest protein expression level was obtained with 293T cells after 96 h of transfection.The relative molecular mass of the purified product was between 100 and 130 kDa was estimated by SDS-PAGE,which was consistent with the expectation.The yield of the purified protein reached 0.8 mg/ml with a purity over 90%.The purified DSG2 extracellular domain protein with IgG1 tag was recognized by IgG monoclonal antibodies by Western blotting.Moreover,the ELISA results showed that the prepared DSG2 extracellular domain protein had significant binding activity to human type 55 adenovirus Fiber Knob protein(HAdV-55).Conclusion:A simple and efficient method for eukaryotic expression and purification of human soluble DSG2 extracellular domain protein was successfully established,and biologically active DSG2 extracellular domain protein was purified,which laid the foundation for the later study of its protein function and anti-adenovirus drugs.

血清可溶性CD36、抵抗素、能量平衡相关蛋白联合预测2型糖尿病合并非酒精性脂肪性肝病的价值探讨

目的探讨血清可溶性CD36(sCD36)、抵抗素、能量平衡相关蛋白(Adropin)联合对2型糖尿病(T2DM)合并非酒精性脂肪性肝病(NAFLD)的预测价值。方法前瞻性选取石家庄市第二医院2018年5月至2021年5月收治的90例T2DM合并NAFLD病人(A组)、90例单纯T2DM病人(B组),同期选取90例体检健康者(对照组)作为研究对象。通过酶联免疫吸附测定检测研究对象血清sCD36、抵抗素、Adropin表达水平,对比分析三组sCD36、抵抗素、Adropin表达水平差异;分析sCD36、抵抗素、Adropin表达相关性;logistic回归分析T2DM合并NAFLD的影响因素;受试者操作特征曲线(ROC曲线)分析sCD36、抵抗素、Adropin三者联合对T2DM合并NAFLD的预测价值。结果A组病人血清sCD36(63.7±15.6)ng/L、抵抗素(3.15±0.46)μg/L水平均高于B组[(43.8±14.2)ng/L、(2.72±0.68)μg/L]和对照组[(22.9±5.7)ng/L、(2.58±0.39)μg/L](P<0.05),血清Adropin水平(66.28±27.62)ng/L均低于B组(86.73±25.46)ng/L和对照组(128.59±45.22)ng/L,差异有统计学意义(P<0.05)。sCD36与抵抗素表达水平呈正相关(r=0.29,P<0.05),Adropin与sCD36表达呈负相关(r=−0.57,P<0.05)。logistic回归分析显示,sCD36,抵抗素,总胆固醇(TC)是T2DM合并NAFLD的影响因素(P<0.05)。ROC曲线分析显示,sCD36、抵抗素、Adropin单独及三者联合预测T2DM合并NAFLD的曲线下面积(AUC)分别为0.81、0.74、0.72、0.89,三者联合对T2DM合并NAFLD的预测效能显著高于单指标独立预测(P<0.05)。结论T2DM合并NAFLD病人血清sCD36、抵抗素水平升高,Adropin水平降低,三者联合对预测T2DM合并NAFLD具有较高效能。

外周血sIL-2R、CD4^(+)/CD8^(+)、TNF-α对初治活动性肺结核老年患者化疗疗效的评估价值

目的探讨外周血可溶性白细胞介素2受体(sIL-2R)、CD4^(+)淋巴细胞百分比/CD8^(+)淋巴细胞百分比比值(下称CD4^(+)/CD8^(+))、肿瘤坏死因子-α(TNF-α)对初治活动性肺结核老年患者化疗疗效的评估价值。方法将2019年12月至2022年12月该院收治的102例初治活动性肺结核老年患者纳入研究作为观察组,另选取102例年龄≥60岁且同期于该院体检的健康者作为对照组。比较两组外周血sIL-2R、TNF-α、CD4^(+)/CD8^(+)水平并分析sIL-2R、TNF-α、CD4^(+)/CD8^(+)间的相关性。观察组均采用2HRZE/4HR抗结核治疗方案,比较观察组治疗前、治疗1个月、治疗6个月时不同疗效患者外周血sIL-2R、TNF-α、CD4^(+)/CD8^(+);分析sIL-2R、CD4^(+)/CD8^(+)、TNF-α水平与疗效的相关性;采用受试者工作特征(ROC)曲线分析各指标用于老年患者化疗疗效评估的效能。结果观察组sIL-2R、TNF-α水平高于对照组,而CD4^(+)/CD8^(+)低于对照组,差异均有统计学意义(P<0.05)。观察组sIL-2R、TNF-α与CD4^(+)/CD8^(+)呈负相关(P<0.05),sIL-2R与TNF-α呈正相关(P<0.05)。治疗1个月、治疗6个月时显效患者sIL-2R、TNF-α水平低于有效患者,而后者又低于无效患者,显效患者CD4^(+)/CD8^(+)高于有效患者,而后者又高于无效患者,差异均有统计学意义(P<0.05)。sIL-2R、TNF-α水平与疗效呈负相关(P<0.05),CD4^(+)/CD8^(+)与疗效呈正相关(P<0.05)。ROC曲线分析显示,治疗1个月、6个月时sIL-2R、CD4^(+)/CD8^(+)、TNF-α联合用于评估疗效的曲线下面积(AUC)明显大于各时间点单项指标用于评估的AUC(P<0.05),而且治疗6个月时各指标联合评估的AUC大于治疗1个月(P<0.05)。结论初治活动性肺结核老年患者sIL-2R、CD4^(+)/CD8^(+)、TNF-α水平与患者疗效密切相关,将以上指标联合用于评估患者化疗疗效具有一定参考价值。

适合致密油藏的超临界CO_(2)-气溶性表面活性剂复合吞吐技术

为了进一步提高致密油储层超临界CO_(2)吞吐的开发效果,探索适合致密油藏的超临界CO_(2)-气溶性表面活性剂复合吞吐技术,通过原油黏度实验、油气界面张力实验、最小混相压力实验、原油膨胀系数实验以及超临界CO_(2)-气溶性表面活性剂复合吞吐模拟实验,评价了不同类型气溶性表面活性剂的性能及其对吞吐采收率的影响。结果表明:气溶性表面活性剂GRS⁃1的综合性能更加突出,随着气溶性表面活性剂质量分数的不断增大,原油黏度、油气界面张力和最小混相压力均呈现出逐渐降低的趋势,而原油体积膨胀系数则逐渐增大,并且岩心的吞吐采收率和入口端压力也呈现出逐渐增大的趋势;随着混合流体注入量的增加以及闷井时间的延长,岩心吞吐采收率和入口端压力均逐渐增大,并且岩心的渗透率越大,吞吐采收率就越高;超临界CO_(2)-气溶性表面活性剂复合吞吐的最佳实验参数为气溶性表面活性剂GRS⁃1的质量分数0.6%、混合流体注入量0.5 PV、闷井时间3 h、吞吐轮次3次。该复合吞吐技术能够显著提高致密油藏的采收率,对高效开发致密油藏具有指导意义。

老年舒张性心力衰竭合并肌少症患者可溶性生长刺激表达基因2蛋白、肌红蛋白、白细胞介素-6水平与心功能的相关性

目的 探讨老年舒张性心力衰竭(DHF)合并肌少症患者外周血可溶性生长刺激表达基因2蛋白(sST2)、肌红蛋白(Myo)、白细胞介素-6(IL-6)水平与心功能的相关性。方法 将122例DHF患者根据有无肌少症分为DHF合并肌少症组60例和DHF组62例,另将健康体检者58例、单纯肌少症患者60例分别纳入对照组、单纯肌少症组,检测各组外周血sST2、Myo、IL-6水平和心功能指标[左室射血分数(LVEF)、心排血量(CO)、心率(HR)、每搏输出量(SV)和心脏指数(CI)]。采用Pearson相关分析法分析sST2、Myo、IL-6与各心功能指标的相关性。绘制受试者工作特征(ROC)曲线,分析sST2、Myo、IL-6单独及联合诊断DHF合并肌少症的效能。结果 与对照组、单纯肌少症组相比,DHF组、DHF合并肌少症组sST2、Myo、IL-6水平和HR均升高,LVEF、CO、SV和CI均降低,差异有统计学意义(P<0.05);与DHF组相比,DHF合并肌少症组sST2、Myo、IL-6水平和HR均升高,LVEF、CO、SV和CI均降低,差异有统计学意义(P<0.05)。sST2、Myo、IL-6均分别与LVEF、CO、SV、CI呈负相关(P<0.001),均与HR呈正相关(P<0.001);sST2、Myo、IL-6、LVEF、SV是DHF合并肌少症的独立影响因素(P<0.05);sST2、Myo、IL-6联合诊断DHF合并肌少症的曲线下面积为0.936,诊断效能优于三者单独检测。结论 老年DHF合并肌少症患者外周血sST2、Myo、IL-6水平显著升高,且sST2、Myo、IL-6均与心功能指标显著相关,三者联合检测对DHF合并肌少症的诊断效能较高。

血清IL-2、sST2表达与特发性膜性肾病免疫抑制剂治疗反应性的相关性

目的探讨特发性膜性肾病患者血清白介素-2(IL-2)、可溶性生长刺激表达基因2蛋白(sST2)表达水平与免疫抑制剂治疗反应性的相关性。方法选取2020年1月至2022年10月于医院接受免疫抑制剂治疗的135例特发性膜性肾病患者,于入院时检测患者血清IL-2、sST2,并于治疗完成后测定24 h尿蛋白定量,依据患者治疗反应性分为缓解组与未缓解组。对比两组患者一般资料及入院时血清IL-2、sST2水平,采用点二列相关性分析血清IL-2、sST2水平与特发性膜性肾病免疫抑制剂治疗反应性的关系,并绘制受试者工作特征(ROC)曲线评估血清IL-2、sST2水平预测特发性膜性肾病免疫抑制剂治疗反应性的价值。结果135例特发性膜性肾病患者中共有132例完成规律治疗,经免疫抑制剂治疗6个月后,101例患者疾病缓解,纳入缓解组,其余31例患者纳入未缓解组。未缓解组年龄、入院时肾功能分级、疾病分期、血清IL-2、sST2水平均高于缓解组,差异有统计学意义(P<0.05);点二列相关性分析显示,血清IL-2、sST2水平与特发性膜性肾病免疫抑制剂治疗反应性不良风险呈正相关(r 1=0.428,P 1<0.001;r 2=0.344,P 2<0.001);绘制ROC曲线,结果显示,血清IL-2、sST2预测特发性膜性肾病免疫抑制剂治疗反应性不良的曲线下面积均>0.7,具有一定预测价值,且联合预测价值更高。结论血清IL-2、sST2表达水平与特发性膜性肾病患者免疫抑制剂治疗反应性密切相关,二者表达水平越高,治疗反应性越差,且联合检测可作为预测特发性膜性肾病患者免疫抑制剂治疗反应性的敏感指标。

Changes in soluble interleukin-2 receptor level in serum and Na^+ -K^+ -exchanging ATPase activity in semen of infertile men caused by antisperm antibody

Aim: To explore the possible mechanisms of male infertility caused by antisperm antibody (AsAb). Methods: Thesoluble interleukin-2 receptor (sIL-2R) level in serum was analyzed by ELISA and Na^+ -K^+ -exchanging ATPase activi-ty in semen by phosphorus (Pi) assay. Results: The slL-2R level in serum was significantly higher and the Na^+ -K^+ -exchanging ATPase activity in semen significantly lower in AsAb positive infertile men when compared with thecontrols. Conclusion: The AsAb titer varies with the slL-2R level in serum. A decrease in Na^+ -K^+ -exchangingATPase activity in semen may play a role in male infertility caused by AsAb.

Increased serum levels of soluble CD146 and vascular endothelial growth factor receptor 2 in patients with exudative age-related macular degeneration

AIM: To investigate serum levels of soluble CD146(s CD146) and vascular endothelial growth factor receptor 2(VEGFR2) in patients with age-related macular degeneration(AMD). METHODS: Eighty-eight patients with exudative AMD and 45 sex-and age-matched healthy controls were enrolled in this study conducted in China. Serum samples was obtained from the patients with exudative AMD and from the controls. Serum sCD146 and VEGFR2 protein levels were measured using an enzyme-linked immunosorbent assay.RESULTS: We found that serum sCD146 and VEGFR2 protein levels were significantly higher in the patients with exudative AMD group than in the controls(t=3.859, P<0.001 and t=3.829, P<0.001, respectively). Serum sCD146 levels were significantly higher in patients with classic choroidal neovascularization(CNV) than in those with occult CNV(t=9.899, P<0.001). There was a significant difference in the trend for exudative AMD in the highest versus lowest quartile of circulating sCD146 levels(χ2=10.29, P=0.001). The receiver operating characteristic curve analysis showed that the area under the curve was 0.696 for s CD146(95%CI: 0.601-0.791) with an optimum diagnostic cut-off value of 157.16 ng/mL, a sensitivity of 55.7%, and a specificity of 82.2%.CONCLUSION: The serum sCD146 level increases and may be a biomarker for exudative AMD.

Serum soluble ST2 is a promising prognostic biomarker in HBV-related acute-on-chronic liver failure

BACKGROUND： The IL-33/ST2 axis is involved in the pathogenesis of many diseases such as autoimmune diseases, cancer,and heart failure. However, studies of the IL-33/ST2 pathway in HBV-related acute-on-chronic liver failure（HBV-ACLF） are lacking. The present study aimed to determine the prognostic role of serum IL-33/soluble ST2（s ST2） in HBV-ACLF.METHODS： Serum levels of IL-33 and sS T2 in healthy controls（HC, n=18）, chronic hepatitis B（CHB, n=27） and HBV-ACLF（n=51） patients at the 1st and 4th week after enrollment were detected using ELISA, and clinical data were collected. The follow-up of HBV-ACLF patients lasted for 6 months at least.RESULTS： There was no significant difference of serum IL-33 level among HC, CHB and HBV-ACLF patients at week 1.However, serum s ST2 level differed significantly among the three groups： highest in the HBV-ACLF group, moderate in the CHB group and lowest in the HC group. There was a reverse correlation between serum s ST2 level and the survival of HBV-ACLF patients. The level of serum s ST2 in HBV-ACLF survivors was significantly declined from week 1 to week 4 following the treatment, whereas that in HBV-ACLF nonsurvivors remained at a high level during the same period. Furthermore, serum sS T2 level was significantly correlated with laboratory parameters and the most updated prognostic scores（CLIF-C OF score, CLIF-C ACLF score and ACLF grades）. Thereceiver operating characteristics curves demonstrated that serum sS T2 level was a good diagnostic marker for predicting the 6-month mortality in HBV-ACLF patients, comparable to the most updated prognostic scores. Serum sS T2 cut-off points for predicting prognosis in HBV-ACLF patients were 76 ng/mL at week 1 or 53 ng/mL at week 4, respectively. HBV-ACLF patients with serum sS T2 level above the cut-off point often had a worse prognosis than those below the cut-off point.CONCLUSION： Serum s ST2 may act as a promising biomarker to assess severity and predict prognosis of patients with HBV-ACLF and help for the early identification and optimal treatment of HBV-ACLF patients at high risk of mortality.

Study of the Effect of Leeching on Plasma Endothelin and Soluble Interleukin-2 Receptor in Patients with Systemic Lupus Erythematosus

Objective: to explore the mechanism of leeching in treating systemic lupus erythematosus (SLE). Methods: Forty-four patients with SLE were randomly divided into conventional corticosteroid treated group (control group, n =20) and conventional treatment group with leeching intervention added (leeching group, n =24). Before and after treatment the concentration of plasma endothelin (ET) and soluble interleukin-2 receptor (sIL-2R) were determined. Results: Before treatment the level of plasma ET and sIL-2R in the SLE patients were all higher than those in the normal healthy group, ( P <0.01). But after treatment the level of these in both groups were significantly improved than those of before treatment ( P <0.05), and comparison between these two treated groups showed that the difference between them was significant ( P <0.05). Conclusion: Leeching added to conventional treatment of SLE could be more effective in improving the level of plasma ET and sIL-2R, and ameliorating the impairment of renal tissues.

采空区碳封存条件下CO_(2)-水界面特性及溶解传质规律

采空区CO_(2)封存作为解决煤炭行业碳排放难题的重要负碳技术储备,在采空区废弃资源二次利用、CO_(2)封存等方面具有广泛的应用前景。利用原位界面张力测定仪开展了不同温压、地层水矿化度及阳离子溶液类型对CO_(2)地层水系统的界面张力(IFT)影响规律实验,明晰了CO_(2)注入含水碎胀煤岩体中的气液界面扩散效应,并将基于统计缔合理论结合兰纳−琼斯势能模型的状态方程(SAFT-LJ状态方程)与密度梯度理论(DGT)结合预测了IFT理论值;利用自主研发的地质封存地化反应模拟实验平台对相同条件下的CO_(2)溶解性进行了探究实验,得到了采空区储层环境下CO_(2)溶解度变化特征,采用D-S模型计算了对应CO_(2)溶解度理论值。实验结果表明:环境温度一定时,采空区储层压力与IFT呈线性负相关关系,储层温度升高,IFT相应增加,但变化幅度较小;温压条件一定时,矿化度与IFT存在正相关性,且在本实验范围内,低压、高温、高矿化度会促使IFT升高;CO_(2)−盐溶液之间的IFT呈现出随着阳离子价态升高而增大的现象(K^(+)<Na^(+)<Ca^(2+)<Mg^(2+));采空区储层压力与CO_(2)溶解度呈正相关关系,当温度为25℃、纯水条件下,压力由0.5 MPa增至2.5 MPa,对应CO_(2)溶解度由0.1627 mol/kg升至0.7141 mol/kg;CO_(2)溶解度随着温度与矿化度的升高而降低;相同质量分数下,一价阳离子溶液(NaCl、KCl)比二价阳离子溶液(CaCl_(2)、MgCl_(2))可溶解更多的CO_(2)。注入采空区中的游离相CO_(2)克服界面张力通过扩散溶解传质作用打破了采空区地层的地球化学平衡,通过明确环境温压条件、采空区水环境对IFT及CO_(2)溶解度的影响规律,阐明CO_(2)地层水气液界面效应及溶解传质机理,以期为采空区CO_(2)封存安全性及封存量评估提供理论基础。

Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

BACKGROUND Acute pancreatitis(AP)is an inflammatory disease in which the regulatory pathway is complex and not well understood.Soluble suppression of tumorigenicity 2(sST2)protein receptor functions as a decoy receptor for interleukin(IL)-33 to prevent IL-33/suppression of tumorigenicity 2L(ST2L)-pathwaymediated T helper(Th)2 immune responses.AIM To investigate the role of sST2 in AP.METHODS We assessed the association between sST2 and severity of AP in 123 patients enrolled in this study.The serum levels of sST2,C-reactive protein(CRP)and Th1-and Th2-related cytokines,including interferon(IFN)-γ,tumor necrosis factor(TNF)-α,IL-2,IL-4,IL-5 and IL-13,were measured by highly sensitive ELISA,and the severity of AP in patients was evaluated by the 2012 Atlanta Classification Criteria.RESULTS Serum sST2 levels were significantly increased in AP patients,and further,these levels were significantly elevated in severe AP(SAP)patients compared to moderately severe AP(MSAP)and mild AP(MAP)patients.Logistic regression showed sST2 was a predictor of SAP[odds ratio(OR):1.003(1.001–1.006),P=0.000].sST2 cutoff point was 1190 pg/mL,and sST2 above this cutoff was associated with SAP.sST2 was also a predictor of any organ failure and mortality during AP[OR:1.006(1.003–1.009),P=0.000,OR:1.002(1.001–1.004),P=0.012,respectively].Additionally,the Th1-related cytokines IFN-γand TNF-αin the SAP group were higher and the Th2-related cytokine IL-4 in the SAP group was significantly lower than those in MSAP and MAP groups.CONCLUSION sST2 may be used as a novel inflammatory marker in predicting AP severity and may regulate the function and differentiation of IL-33/ST2-mediated Th1 and Th2 Lymphocytes in AP homeostasis.

Early cardiopulmonary resuscitation on serum levels of myeloperoxidase,soluble ST2,and hypersensitive C-reactive protein in acute myocardial infarction patients

BACKGROUND Prompt and effective cardiopulmonary resuscitation(CPR)can promote the recovery of spontaneous circulation to some extent and can save patients’lives.The minimum target of cardiac resuscitation is the restoration of spontaneous circulation(ROSC).However,owing to prolonged sudden cardiac arrest,there is relatively high mortality within 24 h after cardiac resuscitation.Moreover,severe cerebral anoxia can deteriorate the prognosis of patients.Therefore,it is important to adopt an effective clinical evaluation of acute myocardial infarct(AMI)patients’prognosis after cardiac resuscitation for the purpose of prevention and management.AIM To investigate early CPR effects on human myeloperoxidase(MPO),soluble ST2(sST2),and hypersensitive C-reactive protein(hs-CRP)levels in AMI patients.METHODS In total,54 patients with cardiac arrest caused by AMI in our hospital were selected as the observation group,and 50 other patients with AMI were selected as the control group.The differences in serum levels of MPO,sST2,and hs-CRP between the observation group and the control group were tested,and the differences in the serum levels of MPO,sST2,and hs-CRP in ROSC and non-ROSC patients,and in patients who died and in those who survived,were analyzed.RESULTS Serum levels of MPO,sST2,hs-CRP,lactic acid,creatine kinase isoenzyme(CKMB),and cardiac troponin I(cTnI)were significantly higher in the observation group than in the control group(P<0.05).Serum levels of MPO,sST2,hs-CRP,lactic acid,CK-MB,and cTnI in the observation group were lower after CPR than before CPR(P<0.05).In the observation group,MPO,sST2,hs-CRP,lactic acid,CK-MB,and cTnI serum levels were lower in ROSC patients than in non-ROSC patients(P<0.05).MPO,sST2,hs-CRP,and lactic acid serum levels of patients who died in the observation group were higher than those of patients who survived(P<0.05).The areas under receiver operating characteristic curve predicted by MPO,sST2,hs-CRP,lactic acid,CK-MB,and cTnI were 0.616,0.681,0.705,0.704,0.702,and 0.656,respectively(P<0.05).The areas under receiver operating characteristic curve for MPO,SST2,hs-CRP,and lactic acid to predict death were 0.724,0.800,0.689,and 0.691,respectively(P<0.05).Logistic regression analysis showed that MPO,sST2,and hs-CRP were the influencing factors of ROSC[odds ratios=1.667,1.589,and 1.409,P<0.05],while MPO,sST2,hs-CRP,and lactic acid were the influencing factors of death(odds ratios=1.624,1.525,1.451,and 1.365,P<0.05).CONCLUSION Serum levels of MPO,sST2,hs-CRP,and lactic acid have a certain value in predicting recovery and prognosis of patients with ROSC.

Elevated pancreatic enzymes, IgM, soluble interleukin-2 receptor in anti-GADab(+) type 1 diabetes

Type 1 diabetes can be classified into immune-mediated diabetes (type 1A) and idiopathic diabetes, which lacks immunological evidence for beta cell autoimmunity (type 1B). Type 1A diabetes is characterized by the presence of the anti-glutamic acid decarboxylase antibody (anti-GADab). Fulminant type 1 diabetes is classified as type 1B diabetes, and characterized by the absence of anti-GADab, flu-like symptoms, and elevated serum exocrine pancreatic enzymes. We report a type 1 diabetic patient who showed flu-like symptoms, elevated serum exocrine pancreatic enzymes, and an extremely high-titer of anti-GADab, manifesting the characteristics of both type 1A and fulminant type 1 diabetes.

Effects of Aprotinin on Serum Interleukin-2 and Soluble Interleukin-2 Receptor during Cardiopulmonary Bypass

Interleukin-2 and its receptor are of importance in regulating immunity responses. The changes of interleukin-2 (IL-2) and soluble interleukin-2 receptor (IL-2R) during heart valve (s) replacement operation and effects of aprotinin on them were observed. Twenty patients undergoing heart valve (s) replacement were randomly divided into two groups: control group (n=10) and apro- tinin group (n=10). In aprotinin group, 1 000 000 KIU aprotinin was given by vein injection and then 2 000 000 KIU was given as a bolus in prime. Blood samples were collected before CPB, right after CPB and on the 1st, 3rd and 7th postoperative day (POD) for serum IL-2 and sIL-2R determination. Results showed that after CPB, IL-2 was reduced and slL-2R increased. Meanwhile, serum IL-2R was lower in aprotinin group than that of control. It is concluded that the immunity depression after CPB is associated with low level of IL-2 and high level of sIL-2R and aprotinin can ameliorate the situation.

急性缺血性脑卒中患者血清可溶性生长刺激表达基因2蛋白、脑钠肽与颈动脉粥样硬化程度的相关性

目的分析急性缺血性脑卒中患者血清可溶性生长刺激表达基因2蛋白(sST2)、脑钠肽(BNP)与颈动脉粥样硬化程度的相关性。方法选取205例急性缺血性脑卒中患者为研究组,并根据颈动脉粥样硬化程度将其分为轻度组100例、中度组70例和重度组35例。另选同期接受体检的150例健康人员为对照组。比较各组血清sST2、BNP水平。分析sST2、BNP水平与颈动脉粥样硬化程度间的相关性。分析急性缺血性脑卒中患者颈动脉粥样硬化程度的影响因素。分析sST2、BNP联合评估对急性缺血性脑卒中患者颈动脉粥样硬化程度的预测价值。结果研究组的血清sST2水平低于对照组,BNP水平高于对照组,差异有统计学意义(P<0.05)。中度组、重度组的sST2水平低于轻度组,BNP水平高于轻度组,且重度组BNP水平高于中度组,sST2水平低于中度组,差异有统计学意义(P<0.05)。急性缺血性脑卒中患者颈动脉粥样硬化程度与其年龄、有无高血压史、有无吸烟史、有无糖尿病史及总胆固醇(TC)、C反应蛋白(CRP)、同型半胱氨酸(Hcy)、低密度脂蛋白(LDL)水平有关(P<0.05)。年龄、高血压史、吸烟史、糖尿病史、TC、CRP、Hcy、LDL、BNP、sST2为患者颈动脉粥样硬化程度的影响因素(P<0.05)。血清BNP水平与颈动脉粥样硬化程度呈正相关,sST2与颈动脉粥样硬化程度呈负相关(P<0.001)。sST2、BNP联合预测的曲线下面积(AUC)大于sST2、BNP单独预测,差异有统计学意义(P<0.001)。结论临床观察急性缺血性脑卒中患者的血清sST2、BNP水平变化,可有效评估其疾病发展情况,有利于改善患者颈动脉粥样硬化程度。sST2、BNP联合评估对急性缺血性脑卒中患者颈动脉粥样硬化程度的诊断效能较好。

Water-soluble lipopolymer delivery of N-methyl-D-aspartic acid receptor 2B siRNA relieves chronic neuropathic pain in rats

Spinal dorsal horn N-Methyl-D-aspartic acid receptor 2B （NR2B） overexpression plays an important role in the production and maintenance of neuropathic pain. Because small interfering RNA （siRNA） can inhibit NR2B expression, siRNA may provide a novel approach to treat neuropathic pain and possibly nerve injury. However, an efficient and safe vector for NR2B siRNA has not been discovered. This study shows that a water soluble lipopolymer （WSLP） comprised of low molecular weight polyethyleneimine （PEI） and cholesterol can deliver siRNA targeting NR2B for the treatment of neuropathic pain. Results show that intrathecal injection of WSLP/siRNA complexes for 3 days inhibit NR2B gene expression with reductions in mRNA and protein levels by 59% and 54%, respectively, compared with control rats （P 〈 0.01）. Injection of WSLP complexed with scrambled siRNA, or PEI with siRNA did not show this inhibitory effect. Moreover, injection of WSLP/siRNA complexes significantly relieved neuropathic pain at 3, 7, 12, and 21 days, while injection of WSLP with scrambled siRNA or PEI with siRNA did not. These results demonstrate that WSLP can efficiently deliver siRNA targeting NR2B in vivo and relieve neuropathic pain.

血清sST2、TG水平联合GRACE危险评分对不稳定型心绞痛患者冠脉病变程度的预测价值

目的探讨血清可溶性生长刺激表达基因2蛋白(sST2)、甘油三酯(TG)、全球急性冠脉事件注册研究(GRACE)评分与不稳定型心绞痛(UA)患者冠脉病变程度的相关性,并分析其预测价值。方法回顾性选取我院2020年11月至2022年11月收治的168例UA患者为研究对象,患者入院后均行冠脉造影检查并采用Gensini评分评估冠脉病变严重程度。根据患者病例资料计算GRACE评分并收集相关临床资料,检测血清sST2表达水平。结果根据Gensini评分结果,168例UA患者中轻度病变组49例(29.17%),中度病变组60例(35.71%),高度病变组59例(35.12%)。3组患者间TG、HDL-C、ApoA1、sST2水平和GRACE评分比较差异有统计学意义(P<0.05);经多因素Logistic回归分析表明,TG、sST2水平及GRACE评分是影响冠脉病变严重程度的独立危险因素(P<0.05)。Spearman相关性分析显示,sST2、TG水平及GRACE评分与Gensini评分呈显著正相关(r=0.777、0.842、0.883,均P<0.001)。ROC曲线分析显示sST2、TG、GRACE评分预测UA患者冠脉高度病变的曲线下面积(AUC)分别为0.786、0.719、0.766,三项指标联合预测的AUC为0.897(95%CI 0.849~0.945)。结论sST2、TG水平及GRACE评分是UA患者冠脉病变程度的独立预测因子,sST2、TG、GRACE三项指标联合评估能够显著提高对UA患者冠脉高度病变的预测价值。