The breeding and large-scale application of hybrid rice contribute significantly to the food supply worldwide.Currently,hybrid seed production uses cytoplasmic male sterile(CMS)lines or photoperiod/thermo-sensitive ge...The breeding and large-scale application of hybrid rice contribute significantly to the food supply worldwide.Currently,hybrid seed production uses cytoplasmic male sterile(CMS)lines or photoperiod/thermo-sensitive genic male sterile(PTGMS)lines as female parent.Despite huge successes,both systems have intrinsic problems.CMS systems are mainly restricted by the narrow restorer resources that make it difficult to breed superior hybrids,while PTGMS systems are limited by conditional sterility of the male sterile lines that makes the propagation of both PTGMS seeds and hybrid seeds vulnerable to unpredictable climate changes.Recessive nuclear male sterile(NMS)lines insensitive to environmental conditions are widely distributed and are ideal for hybrid rice breeding and production,but the lack of effective ways to propagate the pure NMS lines in a large scale renders it impossible to use them for hybrid rice production.The development of"the third-generation hybrid rice technology"enables efficient propagation of the pure NMS lines in commercial scale.This paper discusses the establishment of"the thirdgeneration hybrid rice technology"and further innovations.This new technology breaks the limitations of CMS and PTGMS systems and will bring a big leap forward in hybrid rice production.展开更多
为及时了解国内外对光动力杀菌的研究现状、热点和发展趋势,为相关研究领域的研究者提供参考,本文采用文献计量学方法对Web of Science核心数据库中收录的2002~2022年光动力杀菌相关研究文献进行统计分析。同时,结合可视化分析软件Cites...为及时了解国内外对光动力杀菌的研究现状、热点和发展趋势,为相关研究领域的研究者提供参考,本文采用文献计量学方法对Web of Science核心数据库中收录的2002~2022年光动力杀菌相关研究文献进行统计分析。同时,结合可视化分析软件Citespace对不同国家和地区、机构、作者等进行分析,并对光动力杀菌研究的关键词进行聚类分析和突现分析。通过分析可知,在食品领域,光动力灭菌技术的研究方向主要包括光敏剂的选择和改造、光源的选择和优化以及延长食品货架期等;此外,光动力联合其他冷灭菌技术增强灭菌效果也将成为未来光动力在食品领域的一个研究趋势。展开更多
Hybrid seed production technology(SPT)using genic recessive male sterility is of great importance in maize breeding.Here,we report a novel SPT based on a maize unilateral cross-incompatibility gene Zm Ga1F with an ext...Hybrid seed production technology(SPT)using genic recessive male sterility is of great importance in maize breeding.Here,we report a novel SPT based on a maize unilateral cross-incompatibility gene Zm Ga1F with an extremely low transgene transmission rate(TTR).Proper pollen-specific Zm Ga1F expression severely inhibits pollen tube growth leading to no fertilization.The maintainer line harbors a transgene cassette in an ipe1 male sterile background containing IPE1 to restore ipe1 male fertility,Zm Ga1F to prevent transgenic pollen escape,the red fluorescence protein encoding gene Ds Red2 for the separation of male sterile and fertile seeds,and the herbicide-resistant gene Bar for transgenic plant selection.When the maintainer line is selfed,gametes of ipe1/transgene and ipe1/-genotypes are produced,and pollen of the ipe1/transgene genotype is not able to fertilize female gametes due to pollen tube growth inhibition by Zm Ga1F.Subsequently,seeds of ipe1/ipe1 and ipe1/transgene genotypes are produced at a 1:1 ratio and could be separated easily by fluorescence-based seed sorting.Not a single seed emitting fluorescence is detected in more than 200,000 seeds examined demonstrating that the pollen-tube-inhibition(PTI)-based TTR is lower than what has been reported for similar technologies to date.This PTI-based SPT shows promising potential for future maize hybrid seed production.展开更多
基金supported by the National Natural Science Foundation of China(U1901203)Natural Science Foundation of Guangdong Province(2018B030308008 and 2019A1515110671)+2 种基金Major Program of Guangdong Basic and Applied Research(2019B030302006)Shenzhen Commission on Innovation and Technology Programs(JCYJ20180507181837997)China Postdoctoral Science Foundation(2019M662957)。
文摘The breeding and large-scale application of hybrid rice contribute significantly to the food supply worldwide.Currently,hybrid seed production uses cytoplasmic male sterile(CMS)lines or photoperiod/thermo-sensitive genic male sterile(PTGMS)lines as female parent.Despite huge successes,both systems have intrinsic problems.CMS systems are mainly restricted by the narrow restorer resources that make it difficult to breed superior hybrids,while PTGMS systems are limited by conditional sterility of the male sterile lines that makes the propagation of both PTGMS seeds and hybrid seeds vulnerable to unpredictable climate changes.Recessive nuclear male sterile(NMS)lines insensitive to environmental conditions are widely distributed and are ideal for hybrid rice breeding and production,but the lack of effective ways to propagate the pure NMS lines in a large scale renders it impossible to use them for hybrid rice production.The development of"the third-generation hybrid rice technology"enables efficient propagation of the pure NMS lines in commercial scale.This paper discusses the establishment of"the thirdgeneration hybrid rice technology"and further innovations.This new technology breaks the limitations of CMS and PTGMS systems and will bring a big leap forward in hybrid rice production.
文摘为及时了解国内外对光动力杀菌的研究现状、热点和发展趋势,为相关研究领域的研究者提供参考,本文采用文献计量学方法对Web of Science核心数据库中收录的2002~2022年光动力杀菌相关研究文献进行统计分析。同时,结合可视化分析软件Citespace对不同国家和地区、机构、作者等进行分析,并对光动力杀菌研究的关键词进行聚类分析和突现分析。通过分析可知,在食品领域,光动力灭菌技术的研究方向主要包括光敏剂的选择和改造、光源的选择和优化以及延长食品货架期等;此外,光动力联合其他冷灭菌技术增强灭菌效果也将成为未来光动力在食品领域的一个研究趋势。
基金supported by the National Natural Science Foundation of China(32101725,32172058)。
文摘Hybrid seed production technology(SPT)using genic recessive male sterility is of great importance in maize breeding.Here,we report a novel SPT based on a maize unilateral cross-incompatibility gene Zm Ga1F with an extremely low transgene transmission rate(TTR).Proper pollen-specific Zm Ga1F expression severely inhibits pollen tube growth leading to no fertilization.The maintainer line harbors a transgene cassette in an ipe1 male sterile background containing IPE1 to restore ipe1 male fertility,Zm Ga1F to prevent transgenic pollen escape,the red fluorescence protein encoding gene Ds Red2 for the separation of male sterile and fertile seeds,and the herbicide-resistant gene Bar for transgenic plant selection.When the maintainer line is selfed,gametes of ipe1/transgene and ipe1/-genotypes are produced,and pollen of the ipe1/transgene genotype is not able to fertilize female gametes due to pollen tube growth inhibition by Zm Ga1F.Subsequently,seeds of ipe1/ipe1 and ipe1/transgene genotypes are produced at a 1:1 ratio and could be separated easily by fluorescence-based seed sorting.Not a single seed emitting fluorescence is detected in more than 200,000 seeds examined demonstrating that the pollen-tube-inhibition(PTI)-based TTR is lower than what has been reported for similar technologies to date.This PTI-based SPT shows promising potential for future maize hybrid seed production.
